LOCUS AiP1983 5255 bp DNA circular UNA 09-APR-2024 DEFINITION pAAV-AiE0486m_3xC2-minBG-iCre-WPRE-BGHpA. ACCESSION Circular KEYWORDS . SOURCE (AiP1983 - pAAV-eHGT_486m_3xCore-minBG-iCre-WPRE-BGHpA) ORGANISM . FEATURES Location/Qualifiers LTR complement(1..141) /created_by="yoav.ben-simon" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ITR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ITR" /label="ITR" Bashed_enhancer 156..887 /X-Transferred_From="AiE0486m_3xC2" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AiE0486m_3xC 2" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AiE0486m_3xC2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AiE0486m_3xC 2" /label="AiE0486m_3xC2" promoter 894..946 /created_by="yoav.ben-simon" /modified_by="yoav.ben-simon" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Beta Globin minimal promoter" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG promoter (Beta Globin minimal promoter)" /label="minBG promoter" regulatory 953..962 /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="Kozak sequence" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kozak sequence" /modified_by="yoav.ben-simon" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Kozak sequence" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kozak sequence" /label="Kozak sequence" CDS 956..2014 /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="iCre" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; iCre" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="iCre" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; iCre" /label="iCre" regulatory complement(957..966) /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="Kozak sequence" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kozak sequence" /modified_by="yoav.ben-simon" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Kozak sequence" /Transferred_Similarity="90.00%" /Primary_Match="90.00%; Adjusted-Interval-0:0/0; Kozak sequence" /label="Kozak sequence" 3'UTR 2027..2272 /created_by="yoav.ben-simon" /modified_by="yoav.ben-simon" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="WPRE3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; WPRE3" /label="WPRE3" polyA_signal 2286..2489 /created_by="yoav.ben-simon" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="BGH-pA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BGH-pA" /label="BGH-pA" LTR 2518..2658 /created_by="yoav.ben-simon" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ITR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ITR" /label="ITR" rep_origin 2750..3056 /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="f1 Origin" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 Origin" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="f1 Origin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 Origin" /label="f1 Origin" promoter 3470..3574 /gene="bla" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR promoter" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR promoter" /label="AmpR promoter" misc_feature 3505..3512 /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; FRT3 spacer (FASEBJ 2011, 25, 1)" /label="FRT3 spacer (FASEBJ 2011, 25, 1)" CDS 3575..4432 /created_by="yoav.ben-simon" /modified_by="yoav.ben-simon" /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" rep_origin 4583..5250 /vntifkey=33 /note="Derived using Geneious Prime 2024.0.4 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pUC ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori (pUC ori)" /label="pUC\ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtaaaat caaatcagtt gcaaaatcca 181 tacagtcttc cagtttctca gctgtggaaa ctttgtgtct cttcttttcc accaaaacag 241 ctatctcgtc tttcaagtcc ttgctgaaaa aaaaaagtgt taaatttttc tcaatttaga 301 cttccagcaa aacagattct gcttgttgaa aggcattggt gttcatgagt aagtgagttt 361 ccattttatt tcttcacaaa taagttacat gaagtactta aaatcaaatc agttgcaaaa 421 tccatacagt cttccagttt ctcagctgtg gaaactttgt gtctcttctt ttccaccaaa 481 acagctatct cgtctttcaa gtccttgctg aaaaaaaaaa gtgttaaatt tttctcaatt 541 tagacttcca gcaaaacaga ttctgcttgt tgaaaggcat tggtgttcat gagtaagtga 601 gtttccattt tatttcttca caaataagtt acatgaagta cttaaaatca aatcagttgc 661 aaaatccata cagtcttcca gtttctcagc tgtggaaact ttgtgtctct tcttttccac 721 caaaacagct atctcgtctt tcaagtcctt gctgaaaaaa aaaagtgtta aatttttctc 781 aatttagact tccagcaaaa cagattctgc ttgttgaaag gcattggtgt tcatgagtaa 841 gtgagtttcc attttatttc ttcacaaata agttacatga agtacttgag ctcgggctgg 901 gcataaaagt cagggcagag ccatctattg cttacatttg cttctgggat ccgccaccat 961 ggtgcccaag aagaagagga aagtctccaa cctgctgact gtgcaccaaa acctgcctgc 1021 cctccctgtg gatgccacct ctgatgaagt caggaagaac ctgatggaca tgttcaggga 1081 caggcaggcc ttctctgaac acacctggaa gatgctcctg tctgtgtgca gatcctgggc 1141 tgcctggtgc aagctgaaca acaggaaatg gttccctgct gaacctgagg atgtgaggga 1201 ctacctcctg tacctgcaag ccagaggcct ggctgtgaag accatccaac agcacctggg 1261 ccagctcaac atgctgcaca ggagatctgg cctgcctcgc ccttctgact ccaatgctgt 1321 gtccctggtg atgaggagaa tcagaaagga gaatgtggat gctggggaga gagccaagca 1381 ggccctggcc tttgaacgca ctgactttga ccaagtcaga tccctgatgg agaactctga 1441 cagatgccag gacatcagga acctggcctt cctgggcatt gcctacaaca ccctgctgcg 1501 cattgccgaa attgccagaa tcagagtgaa ggacatctcc cgcaccgatg gtgggagaat 1561 gctgatccac attggcagga ccaagaccct ggtgtccaca gctggtgtgg agaaggccct 1621 gtccctgggg gttaccaagc tggtggagag atggatctct gtgtctggtg tggctgatga 1681 ccccaacaac tacctgttct gccgggtcag aaagaatggt gtggctgccc cttctgccac 1741 ctcccaactg tccacccggg ccctggaagg gatctttgag gccacccacc gcctgatcta 1801 tggtgccaag gatgactctg ggcagagata cctggcctgg tctggccact ctgccagagt 1861 gggtgctgcc agggacatgg ccagggctgg tgtgtccatc cctgaaatca tgcaggctgg 1921 tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga aacctggact ctgagactgg 1981 ggccatggtg aggctgctcg aggatgggga ctaagaattc gatatcataa tcaacctctg 2041 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 2101 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 2161 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc 2221 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggctcgagag 2281 atcttcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 2341 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 2401 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 2461 ggaggattgg gaagacaata gcaggcatga gatctcacgt gcggaccgag cggccgcagg 2521 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 2581 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 2641 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 2701 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc 2761 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 2821 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 2881 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 2941 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 3001 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 3061 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg 3121 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 3181 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc 3241 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 3301 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 3361 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 3421 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 3481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 3541 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 3601 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 3661 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 3721 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 3781 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 3841 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 3901 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 3961 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 4021 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 4081 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 4141 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 4201 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 4261 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 4321 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 4381 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 4441 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 4501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 4561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4741 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 5041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 5101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 5161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 5221 gttcctggcc ttttgctggc cttttgctca catgt //