LOCUS       AiP2035                 5215 bp    DNA     circular UNA 09-APR-2024
DEFINITION  pAAV-AiE2182m-minBG-iCre(R297T)-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE       (AiP2035 - pAAV-MGT_E182-minBGpromoter-iCre(R297T)-WPRE-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        166..771
                     /X-Transferred_From="AiE2182m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:12z3-hkqy5ws"">AiE2182m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE2182m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:12z3-hkqy5ws"">AiE2182m</a
                     >"
                     /label="AiE2182m"
     promoter        830..882
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      905..914
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     regulatory      complement(909..918)
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="90.00%"
                     /Primary_Match="90.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             911..1966
                     /vntifkey=21
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="iCre(297T)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vxt-gmqtbht"">iCre(297T)</
                     a>"
                     /label="iCre(297T)"
     3'UTR           1987..2232
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    2246..2449
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2478..2618
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2710..3016
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3430..3534
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3465..3472
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3535..4392
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4543..5210
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgcctga gcagaacatt tgattctcaa atcaaaacac
      181 taggaaagaa aatgccaatt atttcataaa gaaataatca ctgaagtgat ctttcataaa
      241 agcagagcac ctagcatgtc agagggcaag tgacatttat tgacagcact gtgaactaca
      301 tatagttctg taagccgcct gctgccacac taactgggtt tctgagtggg aggccagagc
      361 tgtatggaag gaaacggcga gagaaataat ggaggccaac atatgtttct gttcaagatc
      421 cccaaagttt actaagagtc tgtgcttata aagggggagg cccatccccc gccaatccat
      481 tcttggtgcc tgttgccagc ctgtaggcat atctgctgga tgctgtctca ggaatatctc
      541 aagggcctct cagcaggttg cagtgtcttg gagaagagca gcagtagttg acataacaat
      601 agagccatct aaattggaag actacacccc tgataatctc atattcagtg gcagcaaagt
      661 ctgaatcagc ctgcttcaag gctgagggag gctacatagt tccataatgt tccaatgagt
      721 tatgcataga ctcattcaac attttccatc tccccttttg taattaaaat ggcccgtgaa
      781 agcccctttg gtagcacata ctgaaagcta tttcactgag tttgagctcg ggctgggcat
      841 aaaagtcagg gcagagccat ctattgctta catttgcttc tgggatccag atctttccaa
      901 gctagccacc atggtgccca agaagaagag gaaagtctcc aacctgctga ctgtgcacca
      961 aaacctgcct gccctccctg tggatgccac ctctgatgaa gtcaggaaga acctgatgga
     1021 catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc tgtctgtgtg
     1081 cagatcctgg gctgcctggt gcaagctgaa caacaggaaa tggttccctg ctgaacctga
     1141 ggatgtgagg gactacctcc tgtacctgca agccagaggc ctggctgtga agaccatcca
     1201 acagcacctg ggccagctca acatgctgca caggagatct ggcctgcctc gcccttctga
     1261 ctccaatgct gtgtccctgg tgatgaggag aatcagaaag gagaatgtgg atgctgggga
     1321 gagagccaag caggccctgg cctttgaacg cactgacttt gaccaagtca gatccctgat
     1381 ggagaactct gacagatgcc aggacatcag gaacctggcc ttcctgggca ttgcctacaa
     1441 caccctgctg cgcattgccg aaattgccag aatcagagtg aaggacatct cccgcaccga
     1501 tggtgggaga atgctgatcc acattggcag gaccaagacc ctggtgtcca cagctggtgt
     1561 ggagaaggcc ctgtccctgg gggttaccaa gctggtggag agatggatct ctgtgtctgg
     1621 tgtggctgat gaccccaaca actacctgtt ctgccgggtc agaaagaatg gtgtggctgc
     1681 cccttctgcc acctcccaac tgtccacccg ggccctggaa gggatctttg aggccaccca
     1741 ccgcctgatc tatggtgcca aggatgactc tgggcagaga tacctggcct ggtctggcca
     1801 ctctgccaga gtgggtgctg ccaccgacat ggccagggct ggtgtgtcca tccctgaaat
     1861 catgcaggct ggtggctgga ccaatgtgaa cattgtgatg aactacatca gaaacctgga
     1921 ctctgagact ggggccatgg tgaggctgct cgaagatggg gactgaggcg cgccgaattc
     1981 gatatcataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2041 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2101 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg
     2161 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2221 acaattccgt ggctcgagag atcttcgact gtgccttcta gttgccagcc atctgttgtt
     2281 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2341 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2401 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatga gatctcacgt
     2461 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     2521 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     2581 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     2641 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     2701 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2761 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2821 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2881 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     2941 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3001 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     3061 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3121 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     3181 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3241 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3301 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     3361 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3421 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3481 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3541 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3601 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3661 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3721 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3781 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3841 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3901 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3961 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4021 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4081 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4141 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4201 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4261 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4321 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4381 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4441 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4501 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4561 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4621 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4681 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4741 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4801 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4861 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4921 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4981 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5041 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5101 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5161 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
