LOCUS       Exported                5384 bp ds-DNA     circular SYN 24-JAN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 2, 2024 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      26..73
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff"
     primer_bind     complement(109..142)
                     /label=Vector.REV
                     /note="color: black; sequence: 
                     ACGTCATGTTTAACTGCTAACAAAGCCCGAAAGG; added: 2022-01-24"
     primer_bind     118..159
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     CTTTGTTAGCAGTTAAACATGACGTGCATACAGGGTTTTACG; added: 
                     2022-01-24"
     misc_feature    complement(130..309)
                     /label=Q5JDW7
                     /note="color: #333333; direction: LEFT"
     primer_bind     complement(286..322)
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     AGGAGATATACATATGGCAGAAATGCTGGTTAAGAGC; added: 2022-01-24"
     primer_bind     297..355
                     /label=Vector.FOR
                     /note="color: black; sequence: 
                     GCATTTCTGCCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGG
                     ; added: 2022-01-24"
     CDS             complement(307..309)
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /note="color: #993366"
                     /translation="M"
     RBS             318..323
                     /note="ribosome binding site"
                     /note="color: #a6acb3"
     protein_bind    354..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(379..397)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        710..787
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             788..1870
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             2679..2870
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(3300..3888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             4010..4825
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(4918..5373)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagt taaacatgac gtgcatacag ggttttacga ccttctgcct gtgcacgttt
      181 aattgctttt tcaaccaggg ctttaacttc tgcttccagt gcatcataaa attccggact
      241 aacacgcatt tccggatcaa cgcttttcac gaattctttc actttgctct taaccagcat
      301 ttctgccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg
      361 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatcgatc
      421 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      481 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      541 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
      601 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
      661 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga
      721 atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
      781 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
      841 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
      901 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
      961 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
     1021 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
     1081 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
     1141 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
     1201 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
     1261 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
     1321 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
     1381 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
     1441 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
     1501 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
     1561 cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg ggatacgacg ataccgaaga
     1621 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     1681 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     1741 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     1801 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1861 cgggcagtga gcgcaacgca attaatgtaa gttagctcac tcattaggca ccgggatctc
     1921 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     1981 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     2041 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     2101 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     2161 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc ccacgggtgc
     2221 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
     2281 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
     2341 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
     2401 ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
     2461 cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     2521 tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg
     2581 catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta
     2641 cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg
     2701 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
     2761 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc
     2821 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2881 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2941 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     3001 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     3061 tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     3121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3721 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3961 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     4021 tcaacgggaa acgtcttgct ctaggccgcg attaaattcc aacatggatg ctgatttata
     4081 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
     4141 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     4201 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     4261 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa
     4321 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     4381 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     4441 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     4501 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     4561 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     4621 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     4681 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     4741 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     4801 tttgatgctc gatgagtttt tctaagaatt aattcatgag cggatacata tttgaatgta
     4861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
     4921 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4981 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5041 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5101 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5161 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5221 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5281 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5341 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gcca
//
