LOCUS       Exported               13913 bp ds-DNA     circular SYN 25-APR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13913)
  AUTHORS   VIB Network
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 2, 2024 from SnapGene Viewer 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13913
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        109..412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
     gap             558..574
                     /estimated_length=17
                     /note="color: #dcdcdc"
     intron          691..1707
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     primer_bind     1631..1653
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     1715..1734
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     1770..1789
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        1770..1788
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
     promoter        1803..1821
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff"
     primer_bind     1803..1820
                     /label=SP6
                     /note="SP6 promoter, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     1837..1875
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     tctttttgcaggatcCTCACTATAGGGAGACCCAAGCTG; added: 2023-01-23"
     promoter        1852..1862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1877..4511
                     /codon_start=1
                     /label=LRRC37B chimpanzee
                     /note="color: #993366"
                     /translation="MSWLRFWGPWPLLTWQLLSLLVKEAQPLVWVKDPLQLTSNPLGPP
                     KPWSSRSSHLPWESPHAPAPPAAPGDFDYLGPSASSQMSALPQEPTENLAPFLMELDSA
                     GELPLGPEPFLAAHQDLNEKRTPEERLPEVVPLLDRDQNQALVQLPRLKWVQTTDPDRA
                     AGHQADEILVPLDSKVSRPTKFVVSPKNLKKDLAERWSLAEIVGIPHQLSKPQRQKQTL
                     PDDYLSMDTLYPGSLPPELRVNADEPPGPPEQVGLSQFHLEPKSQNPETLEDIQSSSLQ
                     EEAPAQLLQLPQEVEPSTQQEAPALPPESSMESLAQTPLNHEVTVQPPGEDQAHYNLPK
                     FTVKPADVEVTMTSEPKNETESTQAQQEAPIQPPEEAEPSSTALRTTAPPPEHPEVTLP
                     PSDKGQAQHSHLTEATVQPLDLELSITTEPTTEVKPSPTTEETSAQPPDLGLAITPEPT
                     TEIGHSTALEKTRAPHPDQVQTLHRSLTEVTGPPTKLESSQDSLVQSETAPEEQKASTS
                     TNICELCTCGDETLSCVGLSPKQRLSQVPVPEPDTYNGIFTILNFQGNYISYIDGNVWK
                     AYSWTEKLILSENYLTELPKDSFEGLLYLQYLDLSCNKIRYIERQTFESLPFLQYINLG
                     CNLITKLSLGTFQAWHGMQFLHNLILNRNPLTTVEDPYLFELPALKYLDMGTTHITLTT
                     LKNILTITVELEKLILPSHMVCCLCQFKNSIEAVSKTVKLHCNTACLTNSIHCPEEASV
                     GNPEGAFMKMLQARKQHMSTELTIESEAPSDSSGINLSGFGSDQLEIQLTEQLRSLIPN
                     EDVRKFMSHVIRTLKMECSETHVQGSCAKLMLQTGLLMKLLSEQQEAKALNVEWDTDQQ
                     KTNYINE"
     CDS             4724..4750
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             4724..4750
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     primer_bind     complement(4758..4790)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     ggagaggggcggatcTCGAGGCTGATCAGCGGG; added: 2023-01-23"
     misc_feature    4782..5368
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     primer_bind     complement(4949..4966)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     5176..5195
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             5369..6088
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5369..6085
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5414..5435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(5675..5694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     6022..6043
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    6112..6700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #a6acb3"
     primer_bind     complement(6165..6185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: #a020f0; direction: LEFT"
     CDS             complement(6583..6594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #993366"
                     /translation="IEGR"
     primer_bind     complement(6703..6719)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6704..6720)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
                     /note="color: #a020f0; direction: LEFT"
     LTR             7225..7405
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(7419..7436)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     ggagaggggcggatcTCGAGGCTGATCAGCGGG; added: 2023-01-23"
     primer_bind     complement(7431..7448)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: #a020f0; direction: LEFT"
     polyA_signal    7437..7661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      7707..8135
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(7794..7813)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     8004..8025
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(8144..8164)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: #a020f0; direction: LEFT"
     promoter        8149..8478
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff"
     rep_origin      8329..8464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     8391..8410
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        8526..8573
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
                     /note="color: #ffffff"
     CDS             8592..8966
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #993366"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9096..9217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(9133..9152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     9187..9206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(9266..9282)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(9266..9282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(9279..9301)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9290..9306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9314..9344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    9359..9380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(9497..9514)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      complement(9668..10256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(9748..9767)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: #a020f0; direction: LEFT"
     CDS             complement(10427..11287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11050..11069
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        complement(11288..11392)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(11467..11486)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: #a020f0; direction: LEFT"
     enhancer        11658..12037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
     promoter        12038..12241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        12039..12237
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff"
     primer_bind     12192..12212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: #a020f0; direction: RIGHT"
     LTR             12255..12435
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    12482..12607
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #a6acb3"
     misc_feature    13100..13333
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #a6acb3"
     CDS             13518..13562
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #993366"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(13860..13913,1..64)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #a6acb3"
ORIGIN
        1 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
       61 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaatccgcg ttacataact
      121 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      181 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      241 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      301 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      361 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
      421 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
      481 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg ggggcgcgcg
      541 ccaggsgggg cggggssnnn nnnnnnnnnn nnnngggsga ggcggagagg tgcggcggca
      601 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
      661 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
      721 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
      901 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
      961 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
     1081 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     1141 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
     1201 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
     1261 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg
     1321 gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     1381 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     1441 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     1501 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     1561 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     1621 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     1681 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1741 catcattttg gcaaagaatt gctcgagcgt aatacgactc actataggga gacccaagct
     1801 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat cctcactata
     1861 gggagaccca agctgcatgt cttggctgcg tttctggggc ccatggcccc tccttacgtg
     1921 gcaactattg tctttactag tcaaggaggc tcagcctctg gtgtgggtca aggacccgct
     1981 ccagctgacc tctaaccccc tggggccacc taagccctgg tcttcccgct cctcccatct
     2041 cccgtgggaa tctccccatg cacctgctcc cccagcagcc ccgggggact ttgattacct
     2101 ggggccctct gcttcttcgc agatgtcagc cctgcctcag gaaccaactg aaaatttggc
     2161 tccattcctg atggaattgg attcagctgg agagctgccc ctggggccag agccgttctt
     2221 ggctgcacat caggatttaa atgaaaagcg gactccagaa gaaaggctcc cagaggtggt
     2281 gccgcttctg gaccgggatc agaaccaggc cctagttcag cttcctcgcc tcaagtgggt
     2341 tcaaactaca gatccagatc gggctgcagg tcatcaggca gatgaaatac ttgttccact
     2401 agacagtaag gtttcaagac caaccaaatt tgttgtttcg cccaagaacc tgaagaaaga
     2461 tctagctgaa cgttggagcc ttgctgagat tgttgggatt ccacaccaat tatccaaacc
     2521 tcagcgtcag aaacagactt tgcctgatga ttatttgagt atggacacac tgtatcccgg
     2581 cagcctgcct ccagaactcc gggtgaacgc agatgagcct ccagggcctc ctgagcaagt
     2641 tggactttct caattccatc tagagcccaa aagtcaaaat ccagagaccc ttgaagacat
     2701 ccagtcttct tcactccagg aagaagcccc agcgcagctt ctacagctcc ctcaggaggt
     2761 agaaccttca acccagcagg aggccccagc tctgcctcca gagtcctcta tggagagtct
     2821 agctcaaact ccactgaatc atgaagtgac agttcaacct ccaggtgagg atcaagctca
     2881 ttataatttg cccaagttta cagtcaaacc tgcagatgtg gaggttacca tgacttcaga
     2941 gcctaaaaat gagacagaat ctacccaagc ccagcaggag gccccaattc agcctccaga
     3001 ggaggcggaa ccttcttcta cagccctgag gactacagct cctcctccag aacaccctga
     3061 ggtgacactt ccaccttcag acaagggtca ggctcagcat tcacacctga ctgaagccac
     3121 agttcaacct ctggacctgg agcttagcat aactacagag cctactacag aggttaaacc
     3181 gtctccaacc acggaggaaa cctcagctca gcctccagac ctggggcttg ccataactcc
     3241 ggaacccact acagagattg gacattccac agccctggag aagactagag ctcctcatcc
     3301 agaccaggtt cagactctgc atcgaagcct gactgaagtc acaggtccac ctacaaagtt
     3361 agaatcttcg caggattcat tggtgcagtc tgaaactgca ccagaggaac agaaggcctc
     3421 cacaagcacc aacatatgtg agctctgcac ctgcggagat gagactctgt catgtgttgg
     3481 tctcagccca aagcagaggc tcagccaagt gcctgtgcca gagcccgaca cctacaatgg
     3541 catcttcacc atcttaaatt tccaaggaaa ctatatttca tacattgatg gaaatgtatg
     3601 gaaagcatac agttggaccg agaaactaat tctcagtgaa aattatttga ctgaattacc
     3661 taaggattca tttgaaggcc tgctatacct ccagtattta gatttatcct gcaataaaat
     3721 acgatatatt gaaagacaaa catttgaatc actaccattt ttgcagtata taaatctggg
     3781 ctgcaattta attacaaaac tgagccttgg aacatttcag gcctggcacg gaatgcagtt
     3841 tttacacaac ttaattctca atcgcaatcc tctgactact gtcgaagatc catatctctt
     3901 tgaactgccg gcattaaaat atctagacat gggaacaaca cacatcacac ttacaacact
     3961 taagaacatt ctcacgataa ctgttgaact ggaaaaactg atcttaccta gccatatggt
     4021 ctgctgcctc tgccaattta aaaatagcat tgaggctgtc tccaagacag tcaagctgca
     4081 ttgcaacact gcatgtctga ctaacagcat acattgtcct gaagaagcat ctgtaggcaa
     4141 tccagaagga gcgttcatga agatgttaca agcccggaag cagcacatga gcactgagct
     4201 gactattgag tcggaggcgc cctcagacag cagtggcatc aacttgtcag gctttgggag
     4261 tgatcagctt gaaattcagc taaccgagca gctacggtcc ctcatcccca acgaggatgt
     4321 gagaaagttc atgtctcatg ttatccggac cttgaaaatg gaatgttcag aaacacatgt
     4381 gcaagggagc tgtgccaagc tcatgttgca aacaggcctc ctgatgaagc ttctcagcga
     4441 gcagcaggaa gcaaaggcat tgaatgtaga atgggatacg gaccaacaaa aaacaaatta
     4501 tattaatgag aacatggaac agaatgaaca gaaagagcag aagtcaagtg agctcatgaa
     4561 agaagttcca ggatatgact ataagaacaa actcatcttc gcaatatctg tgactgtaat
     4621 actaataatt ttgattataa ttttttgtct tatagaggtg aatgcacata aaagggcatc
     4681 agaaaaatac aaagacaacc catcaatatc aggaggcctc gagtacccat acgatgttcc
     4741 agattacgct tgataaaccc gctgatcagc ctcgagatcc gcccctctcc ctcccccccc
     4801 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     4861 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     4921 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4981 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     5041 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     5101 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     5161 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     5221 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt
     5281 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5341 aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg
     5401 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5461 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5521 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     5581 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     5641 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     5701 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5761 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     5821 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5881 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5941 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     6001 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6061 tctcggcatg gacgagctgt acaagtaaaa ttcgatatca agcttatcga taatcaacct
     6121 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6181 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6241 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6301 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6361 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6421 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6481 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6541 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6601 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6661 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     6721 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     6781 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     6841 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6901 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     6961 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     7021 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     7081 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     7141 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     7201 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     7261 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7321 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7381 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     7441 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7501 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7561 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7621 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7681 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7741 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7801 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7861 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7921 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7981 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8041 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8101 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     8161 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8221 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8281 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8341 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8401 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8461 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     8521 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     8581 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     8641 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     8701 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     8761 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     8821 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     8881 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     8941 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     9001 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     9061 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     9121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     9241 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9421 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9481 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9601 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9661 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9721 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9781 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9841 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9901 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9961 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10021 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10081 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10141 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10201 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10261 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10321 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10381 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10441 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10501 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10561 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10621 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10681 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10741 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10801 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10861 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10921 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10981 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11041 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11101 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11161 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11221 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11281 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11341 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11401 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
    11461 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    11521 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    11581 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    11641 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
    11701 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
    11761 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
    11821 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
    11881 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
    11941 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
    12001 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
    12061 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
    12121 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
    12181 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
    12241 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    12301 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    12361 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    12421 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
    12481 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
    12541 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
    12601 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
    12661 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
    12721 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
    12781 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
    12841 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
    12901 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
    12961 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
    13021 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
    13081 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
    13141 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
    13201 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
    13261 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    13321 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    13381 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    13441 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    13501 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    13561 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    13621 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    13681 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
    13741 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
    13801 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
    13861 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt aga
//
