LOCUS       Exported                9578 bp ds-DNA     circular SYN 18-JUN-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    BLP8_pLV_H1_shSRGAP2BC#2_CAG_Venus.gb
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2018 from SnapGene 4.0.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          292..1538
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    complement(1..76)
                     /label=SRGAP2BC_shRNA2
     misc_feature    complement(77..291)
                     /label=H1 promoter
                     /note="H1 promoter"
     primer_bind     complement(137..156)
                     /label=H1 primer
     primer_bind     292..312
                     /label=Fragment.FOR
     promoter        297..768
                     /label=hSynapsin I promoter
     CDS             786..1505
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1482..1519)
                     /label=Fragment.REV
     CDS             1506..1515
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VQV"
     polyA_signal    1539..2140
                     /label=WRE
                     /note="Enhances stability of transcripts
                     vntifkey=25"
     primer_bind     complement(1809..1828)
                     /label=pLenti-Rv1
     primer_bind     complement(1930..1949)
                     /label=WRE-Rv
     LTR             2658..2838
                     /label=LTR (1)
                     /label=LTR(1)
                     /note="/vntifkey=19"
     rep_origin      3157..3463
                     /label=F1 ori
     primer_bind     complement(4695..4715)
                     /label=M13-rev(1)
     misc_binding    complement(4721..4743)
                     /label=LacO(1)
     rep_origin      5042..5715
                     /label=Ori
                     /note="/vntifkey=33"
     rep_origin      complement(5083..5765)
                     /direction=LEFT
                     /label=ColE1 origin
     mRNA            5860..6720
                     /label=Ampicillin
                     /note="/vntifkey=54"
     CDS             complement(5863..6522)
                     /label=AmpR
     LTR             7688..7868
                     /label=LTR
                     /note="/vntifkey=19"
     misc_feature    7979..8123
                     /label=HIV-psi pack. signal
                     /label=nonstandard type: virion
                     /note="/vntifkey=97"
     misc_feature    8533..8766
                     /label=REE
                     /label=nonstandard type: provirus
                     /note="HIV Rev responsive element
                     vntifkey=92"
     primer_bind     9254..9273
                     /label=pLenti-Fw1
     primer_bind     9423..9442
                     /label=pLenti-Fw2
     primer_bind     9452..9472
                     /label=seq-primer_for_pSCV2
     misc_binding    9516..9538
                     /label=LacO
     primer_bind     9544..9564
                     /label=M13-rev
ORIGIN
        1 ctagcaaaaa aaaggacagg cattgaatat cttagtgaca ggaagctaag atattcaatg
       61 cctgtccttg ggatccgtgg tctcatacag aacttataag attcccaaat ccaaagacat
      121 ttcacgttta tggtgatttc ccagaacaca tagcgacatg caaatattgc agggcgccac
      181 tcccctgtcc ctcacagcca tcttcctgcc agggcgcacg cgcgctgggt gttcccgcct
      241 agtgacactg ggcccgcgat tccttggagc gggttgatga cgtcagcgtt cgaattctgc
      301 agagggccct gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt
      361 gcctacctga cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt
      421 gcgcatcccc tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg
      481 ccagcttcag caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc
      541 ctcagcactg aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc
      601 caccttggtc gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag
      661 ataggggggc acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag
      721 tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aaaccggtcg
      781 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      841 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      901 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      961 ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac cccgaccaca
     1021 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1081 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1141 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1201 ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga
     1261 agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc
     1321 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1381 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     1441 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1501 agtaagtaca agtaaaattc gatatcaagc ttatcgatat cgataatcaa cctctggatt
     1561 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     1621 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     1681 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     1741 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     1801 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     1861 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     1921 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     1981 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     2041 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     2101 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     2161 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     2221 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     2281 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     2341 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     2401 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     2461 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     2521 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     2581 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     2641 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2701 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2821 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2881 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2941 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3001 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3061 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     3121 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3481 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3541 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3601 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3661 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3721 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3781 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3841 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3901 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     3961 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     4021 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     4081 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     4141 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     4201 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     4261 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     4321 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     4381 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     4441 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4501 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     4561 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4621 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     4681 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6841 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     6901 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     6961 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     7021 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     7081 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     7141 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     7201 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     7261 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     7321 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     7381 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     7441 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     7501 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     7561 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     7621 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     7681 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7741 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7801 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7861 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7921 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7981 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8041 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8101 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8161 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     8221 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8281 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8341 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8401 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8461 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8521 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8581 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8641 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8701 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8761 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     8821 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8881 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     8941 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9001 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9061 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9121 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     9181 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     9241 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     9301 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9361 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9421 acagggacag cagagatcca gtttggttaa tgagttagct cactcattag gcaccccagg
     9481 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9541 acacaggaaa cagctatgac catgattacg ccaagctg
//
