LOCUS       Exported                9574 bp ds-DNA     circular SYN 18-JUN-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    BLP6_pLV_H1_shSRGAP2A_CAG_Venus.gb
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2018 from SnapGene 4.0.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          288..1534
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    complement(1..72)
                     /label=SRGAP2A_shRNA
     misc_feature    complement(73..287)
                     /label=H1 promoter
                     /note="H1 promoter"
     primer_bind     complement(133..152)
                     /label=H1 primer
     primer_bind     288..308
                     /label=Fragment.FOR
     promoter        293..764
                     /label=hSynapsin I promoter
     CDS             782..1501
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1478..1515)
                     /label=Fragment.REV
     CDS             1502..1511
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VQV"
     polyA_signal    1535..2136
                     /label=WRE
                     /note="Enhances stability of transcripts
                     vntifkey=25"
     primer_bind     complement(1805..1824)
                     /label=pLenti-Rv1
     primer_bind     complement(1926..1945)
                     /label=WRE-Rv
     LTR             2654..2834
                     /label=LTR (1)
                     /label=LTR(1)
                     /note="/vntifkey=19"
     rep_origin      3153..3459
                     /label=F1 ori
     primer_bind     complement(4691..4711)
                     /label=M13-rev(1)
     misc_binding    complement(4717..4739)
                     /label=LacO(1)
     rep_origin      5038..5711
                     /label=Ori
                     /note="/vntifkey=33"
     rep_origin      complement(5079..5761)
                     /direction=LEFT
                     /label=ColE1 origin
     mRNA            5856..6716
                     /label=Ampicillin
                     /note="/vntifkey=54"
     CDS             complement(5859..6518)
                     /label=AmpR
     LTR             7684..7864
                     /label=LTR
                     /note="/vntifkey=19"
     misc_feature    7975..8119
                     /label=HIV-psi pack. signal
                     /label=nonstandard type: virion
                     /note="/vntifkey=97"
     misc_feature    8529..8762
                     /label=REE
                     /label=nonstandard type: provirus
                     /note="HIV Rev responsive element
                     vntifkey=92"
     primer_bind     9250..9269
                     /label=pLenti-Fw1
     primer_bind     9419..9438
                     /label=pLenti-Fw2
     primer_bind     9448..9468
                     /label=seq-primer_for_pSCV2
     misc_binding    9512..9534
                     /label=LacO
     primer_bind     9540..9560
                     /label=M13-rev
ORIGIN
        1 ctagcaaaaa agccactcat ccctgaagaa tcgtgacagg aagcgattct tcagggatga
       61 gtggcgggat ccgtggtctc atacagaact tataagattc ccaaatccaa agacatttca
      121 cgtttatggt gatttcccag aacacatagc gacatgcaaa tattgcaggg cgccactccc
      181 ctgtccctca cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg
      241 acactgggcc cgcgattcct tggagcgggt tgatgacgtc agcgttcgaa ttctgcagag
      301 ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt gggggtgcct
      361 acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc
      421 atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag
      481 cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca
      541 gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt cccggccacc
      601 ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag
      661 gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg
      721 cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgagaaac cggtcgccac
      781 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      841 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      901 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      961 cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     1021 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1081 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1141 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1201 caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa
     1261 cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc
     1321 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1381 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     1441 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     1501 agtacaagta aaattcgata tcaagcttat cgatatcgat aatcaacctc tggattacaa
     1561 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     1621 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     1681 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     1741 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     1801 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     1861 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     1921 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     1981 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2041 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2101 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     2161 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     2221 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     2281 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     2341 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     2401 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     2461 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     2521 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     2581 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     2641 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2701 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2761 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2821 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     2881 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2941 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3001 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3061 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     3121 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3181 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3241 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3301 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3361 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3421 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3481 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3541 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     3601 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3661 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3721 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3781 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3841 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3901 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     3961 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     4021 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     4081 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     4141 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     4201 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     4261 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     4321 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     4381 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4441 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4501 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     4561 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4621 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     4681 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4741 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4801 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6721 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6781 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6841 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     6901 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     6961 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     7021 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     7081 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     7141 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     7201 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     7261 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     7321 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     7381 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     7441 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     7501 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     7561 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     7621 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     7681 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7741 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7801 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7861 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     7921 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     7981 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     8041 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     8101 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     8161 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     8221 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     8281 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8341 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     8401 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     8461 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     8521 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     8581 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     8641 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     8701 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     8761 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     8821 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     8881 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     8941 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     9001 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     9061 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     9121 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     9181 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     9241 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     9301 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     9361 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     9421 ggacagcaga gatccagttt ggttaatgag ttagctcact cattaggcac cccaggcttt
     9481 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9541 aggaaacagc tatgaccatg attacgccaa gctg
//
