LOCUS       Exported                3740 bp ds-DNA     circular SYN 11-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 2, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..17
                     /label=NSP5 SA11
     misc_feature    68..156
                     /label=Ribozima
     misc_feature    68..156
                     /label=Ribozima cc
     misc_feature    158..165
                     /label=8 A
     misc_feature    172..310
                     /label=T7 terminator
     primer_bind     complement(216..234)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      230..277
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(345..363)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(346..363)
                     /label=SP6
     primer_bind     complement(346..363)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(653..672)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(882..901)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1061..1079)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1147..1251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1252..2112
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1470..1489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2261..2889
                     /direction=RIGHT
                     /label=ColE1 origin
     rep_origin      2283..2871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2772..2791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3025..3042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3126..3144
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3126..3142
                     /label=T7 promoter senza GG
     misc_feature    3143..3163
                     /label=5 UTR 2g
     misc_feature    3167..3181
                     /label=NSP5 SA11
ORIGIN
        1 aattgattga agatttgtaa gtctgacctg ggaacacact agggagctcc ccactcccgt
       61 tttgtgaccg ggtcggcatg gcatctccac ctcctcgcgg tccgacctgg gcatccgaag
      121 gaggacgtcg tccactcgga tggctaaggg agagctcaaa aaaaaggatc cggctgctaa
      181 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      241 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      301 atcgagatcc tctagagtcg acctgcaggc atgcaagctt gtattctata gtgtcaccta
      361 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
      421 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
      481 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
      541 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
      601 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      661 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      721 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcac
      781 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcttaatgc
      841 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
      901 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     1081 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1141 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1261 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1321 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1381 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1441 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1501 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1561 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1621 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1681 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1741 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1801 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1861 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1921 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1981 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2041 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2101 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2161 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2221 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2281 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2341 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2401 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2461 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2521 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2581 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2641 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2701 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2761 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2821 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2881 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2941 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3001 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3061 atacgcaaac cgcctctccc cgcgcattgg ccgattcatt aatgcagggg gatatcgatc
     3121 ccggttaata cgactcacta taggctttta aagcgctaca gtgatgtctc tcagtattga
     3181 cgtgacgagt cttctcaaag taacttaaac tatatataag aattaatcgt cttcctcaaa
     3241 ctttaacaat tctggaaaaa atattggtaa gagtgaacag aacatttcac cagaaccaaa
     3301 agcattcaat aaatacctcc tgtcccaaaa aaaagagaaa ctagcccaat taaaaactgc
     3361 ttcaaaaaat acactcatcg aattaacgat taggtcgaat tcagttaaga aaaatgcccc
     3421 cagacccgaa aatacggaaa gaacagctca aaaaagacct taaattattg tctaataact
     3481 tcaagtaaaa ttctccttaa ataaaggctt aaaagtaaaa gctaatttgg actcatcaat
     3541 atcaatatct acggatacta aaaaggagaa atcaaaacaa aaccataaaa gtaggaagca
     3601 ctacccaaga attgaagcag agtctgattc agatgattat gtactggatg attcagatag
     3661 tgatgatggt aaatgtaaga actgtaaata taagaagaaa tacttcgcat taagaatgag
     3721 aatgaaacaa gtcgcaatgc
//
