LOCUS       pENN239_cod.opt._cag-H 11061 bp ds-DNA     circular     13-OCT-2023
DEFINITION  .
KEYWORDS    "creator:Edwin Neumann" "marker:AmpR, AmpR, B11-151217-R,
            A1-151002-R, B10-151217-R, 152028_CG_hISL1_5R"
FEATURES             Location/Qualifiers
     enhancer        1..380
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: CMV enhancer"
     primer_bind     complement(215..233)
                     /label="B10-151217-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: B10-151217-R"
     promoter        383..658
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: chicken β-actin promoter"
     primer_bind     403..422
                     /label="B16-160126-F"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="label: B16-160126-F"
     primer_bind     627..646
                     /label="B03-151217-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: B03-151217-F"
     intron          659..1667
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: chimeric intron"
     misc_feature    1223..1231
                     /label="Kozac"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="label: Kozac"
     primer_bind     1377..1397
                     /label="B04-151217-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: B04-151217-F"
     primer_bind     1624..1644
                     /label="A41-151006"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: A41-151006"
     misc_feature    1624..1644
                     /label="GCPo62"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: GCPo62"
     intron          1626..1719
                     /label="Mini-Intron"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: Mini-Intron"
     primer_bind     1629..1649
                     /label="AA27-151016-1F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: AA27-151016-1F"
     primer_bind     1683..1701
                     /label="A2-151002-F"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: A2-151002-F"
     misc_feature    1693..1726
                     /label="xENN162"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1741..1753
                     /label="JKN1093"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: JKN1093"
     CDS             1766..2617
                     /label="Translation 1766-2617"
                     /translation="MARTKQTARKSTGGKAPRKQLATKAARKSAPGGASSGAGSSSGGSAAGSGSGGASSGAASSSAGSAAGSGSSPVEIYKTVSAWKRQPIRVLSLFGNIDKELKSLGFLESGSHSEGGTLKYVEDVTNVVRRDVEKWGPFDLLYGSTQPLGGSCDRCPGWYMFQFHRILQYARPRQESQPFFWIFMDNLLLTEDDQMTTVRFLQTEAVTLRDVRSRVLQNAVRVWSNIPGLKSKHEALSPKEEESLQGHVRTRSKLAAPKVDPLVKNCLLPLREYFKYFSQSSLPL"
     misc_feature    1769..1858
                     /label="H3 tail ext"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1859..1978
                     /label="maxiflex40 (pENN175 codons)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1979..2617
                     /label="AsDnmt3L"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 transl_table: 1 translation: MGPMEIYKTVSAWKRQPVRVLSLFRNIDKVLKSLGFLESGSGSGGGTLKYVEDVTNVVRRDVEKWGPFDLVYGSTQPLGSSCDRCPGWYMFQFHRILQYALPRQESQRPFFWIFMDNLLLTEDDQETTTRFLQTEAVTLQDVRGRDYQNAMRVWSNIPGLKSKHAPLTPKEEEYLQAQVRSRSKLDAPKVDLLVKNCLLPLREYFKYFSQNSLPL"
     CDS             2618..2857
                     /label="XTEN80"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="codon_start: 1 transl_table: 1 translation: GGPSSGAPPPSGGSPAGSPTSTEEGTSESATPESGPGTSTEPSEGSAPGSPAGSPTSTEEGTSTEPSEGSAPGTSTEPSE"
     CDS             2618..7237
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GGPSSGAPPPSGGSPAGSPTSTEEGTSESATPESGPGTSTEPSEGSAPGSPAGSPTSTEEGTSTEPSEGSAPGTSTEPSEPKKKRKVMDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDPKKKRKVSGSETPGTSESATPESRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEP"
     CDS             2858..2878
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 locus_tag: note: product: nuclear localization signal of SV40 (simian virus 40) large T antigen protein_id: transl_table: 1 translation: PKKKRKV"
     CDS             2879..6982
                     /label="Cod.opt.dCas9"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 note: From Luke Koblan transl_table: 1 translation: MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     gene            2906..2908
                     /label="D10A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: D10A note: /created_by=lkoblan"
     gene            5396..5398
                     /label="H840A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: H840A note: /created_by=lkoblan note: /modified_by=lkoblan"
     CDS             6983..7003
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 locus_tag: note: product: nuclear localization signal of SV40 (simian virus 40) large T antigen protein_id: transl_table: 1 translation: PKKKRKV"
     CDS             7004..7051
                     /label="XTEN16"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="codon_start: 1 transl_table: 1 translation: SGSETPGTSESATPES"
     CDS             7052..7237
                     /label="KRAB"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: product: Krüppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994) protein_id: transl_table: 1 translation: RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEP"
     misc_feature    7238..7257
                     /label="anneal F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             7238..7294
                     /label="P2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. product: 2A peptide from porcine teschovirus-1 polyprotein transl_table: 1 translation: ATNFSLLKQAGDVEENPGP"
     CDS             7295..7993
                     /label="TagBFP"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
                     /note="codon_start: 1 transl_table: 1 translation: SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN*"
     misc_feature    complement(7971..7996)
                     /label="anneal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(8001..8020)
                     /label="homology"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     8016..8026
                     /label="152028_CG_hISL1_5R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 152028_CG_hISL1_5R"
     primer_bind     complement(8115..8134)
                     /label="A1-151002-R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: A1-151002-R"
     misc_feature    8124..8149
                     /label="5UTR F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     polyA_site      8147..8202
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: β-globin poly(A) signal"
     primer_bind     complement(8162..8184)
                     /label="B11-151217-R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="label: B11-151217-R"
     misc_feature    complement(8549..8574)
                     /label="5UTR R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(8561..8577)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="label: M13 rev"
     protein_bind    8585..8601
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="label: lac operator"
     primer_bind     8589..8608
                     /label="B19-160210"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: B19-160210"
     primer_bind     complement(8589..8608)
                     /label="A18-MM-SV40PA_1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: A18-MM-SV40PA_1"
     primer_bind     complement(8589..8608)
                     /label="pLac(F)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: pLac(F)"
     promoter        complement(8609..8615)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: lac promoter"
     promoter        complement(8616..8633)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: lac promoter"
     promoter        complement(8634..8639)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: lac promoter"
     protein_bind    8654..8675
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="label: CAP binding site"
     promoter        8733..8929
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: SV40 promoter"
     rep_origin      8780..8915
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: SV40 ori"
     polyA_signal    8935..9065
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: SV40 pA"
     polyA_signal    8935..9069
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: SV40 poly(A) signal"
     primer_bind     complement(9044..9063)
                     /label="C31"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="label: C31"
     rep_origin      complement(9308..9896)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT label: ori"
     primer_bind     complement(9369..9389)
                     /label="E61"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="label: E61"
     primer_bind     complement(9933..9952)
                     /label="E60"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="label: E60"
     CDS             complement(10067..10858)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR"
     CDS             complement(10070..10927)
                     /label="Amp"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: Amp"
     primer_bind     complement(10456..10476)
                     /label="E59"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: E59"
     primer_bind     10772..10791
                     /label="F4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: F4"
     misc_feature    10786..10809
                     /label="GCPo87"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: GCPo87"
     misc_feature    complement(10786..10809)
                     /label="GCPo94"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: GCPo94"
     CDS             complement(10859..10927)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR"
     primer_bind     complement(10917..10944)
                     /label="HH28"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="label: HH28"
     promoter        complement(10928..11032)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: AmpR promoter"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      421 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      481 atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg
      721 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      781 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct
      841 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt
      901 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg
      961 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt
     1021 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1081 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct
     1141 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg
     1201 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1261 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc
     1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1381 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag
     1441 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     1501 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg
     1561 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1621 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1681 tgctggttat tgTGCTGTCT CATCATTTTG GCAAAGAATT CTGCAGTCGA CGGTACCGCG
     1741 GGCCCGGGAT CCACCGGTCG CCACCATGGC CAGGACCAAA CAAACTGCCA GAAAGAGCAC
     1801 CGGGGGGAAA GCGCCCCGAA AACAGCTGGC GACCAAAGCA GCCCGCAAAA GCGCACCGGG
     1861 AGGTGCAAGC AGTGGGGCAG GAAGTTCCTC AGGAGGAAGT GCGGCGGGAT CCGGAAGCGG
     1921 TGGCGCATCA AGTGGCGCCG CAAGTAGCAG TGCCGGGTCC GCAGCTGGCT CCGGGTCCTC
     1981 CCCTGTTGAG ATCTACAAGA CCGTGTCCGC CTGGAAGCGG CAGCCAATCA GAGTGCTGAG
     2041 CCTGTTCGGC AATATTGACA AGGAACTGAA GTCTCTGGGC TTCCTGGAAA GCGGCAGCCA
     2101 CAGCGAGGGC GGCACACTGA AGTACGTGGA AGATGTGACC AACGTGGTGC GGAGAGATGT
     2161 GGAAAAGTGG GGACCTTTCG ACCTGCTGTA CGGCAGCACC CAGCCTCTGG GCGGAAGCTG
     2221 CGACAGGTGT CCTGGCTGGT ACATGTTCCA ATTTCATAGA ATCCTGCAGT ACGCCAGACC
     2281 TAGACAGGAG TCTCAGCCCT TCTTCTGGAT CTTCATGGAT AACCTGCTGC TCACAGAGGA
     2341 CGACCAGATG ACCACCGTGC GGTTCCTGCA AACAGAGGCC GTCACCCTGC GCGACGTGAG
     2401 ATCTAGAGTG CTGCAGAACG CCGTCCGGGT GTGGAGCAAC ATCCCCGGTC TTAAAAGCAA
     2461 GCACGAGGCT CTGTCCCCAA AAGAGGAAGA GAGCCTGCAG GGCCACGTGC GGACCAGATC
     2521 TAAGCTGGCC GCTCCTAAAG TGGACCCCCT GGTGAAGAAC TGCCTGCTGC CTCTGAGAGA
     2581 GTACTTTAAG TATTTCAGCC AGAGCAGCCT GCCCCTGGGC GGCCCTTCTA GCGGTGCTCC
     2641 ACCTCCAAGC GGCGGCTCGC CAGCCGGCAG CCCTACCAGC ACCGAGGAGG GCACAAGCGA
     2701 GAGCGCAACA CCTGAGAGCG GCCCCGGCAC AAGCACCGAG CCTTCTGAAG GAAGCGCCCC
     2761 TGGGAGCCCT GCCGGCAGCC CCACCAGCAC AGAAGAGGGC ACCTCTACCG AGCCCAGCGA
     2821 GGGTAGCGCC CCTGGCACAA GCACAGAACC CTCTGAGCCT AAGAAAAAGC GGAAGGTGAT
     2881 Ggacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat
     2941 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
     3001 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     3061 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     3121 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     3181 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     3241 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     3301 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     3361 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     3421 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     3481 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     3541 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     3601 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     3661 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     3721 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     3781 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     3841 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     3901 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     3961 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     4021 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     4081 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     4141 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     4201 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     4261 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     4321 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     4381 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     4441 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     4501 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     4561 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     4621 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     4681 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     4741 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     4801 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     4861 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     4921 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     4981 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     5041 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     5101 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     5161 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     5221 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     5281 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     5341 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat
     5401 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     5461 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     5521 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     5581 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     5641 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     5701 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     5761 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     5821 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     5881 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     5941 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     6001 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     6061 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     6121 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     6181 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     6241 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     6301 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     6361 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     6421 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     6481 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     6541 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     6601 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     6661 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     6721 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     6781 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     6841 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     6901 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     6961 cctgtctcag ctgggaggtg acCCCAAGAA GAAGCGGAAG GTGTCCGGCT CTGAAACCCC
     7021 TGGAACCTCC GAGAGCGCCA CACCTGAAAG CAGAACCCTG GTGACCTTCA AGGACGTGTT
     7081 CGTGGACTTC ACCCGCGAGG AATGGAAGCT CCTGGACACC GCCCAGCAAA TCGTGTACCG
     7141 GAACGTGATG CTGGAGAATT ACAAGAACCT GGTGTCTCTG GGCTACCAGC TGACGAAGCC
     7201 TGACGTCATC CTGAGACTGG AAAAGGGAGA GGAGCCTGCC ACAAATTTCA GCCTGCTGAA
     7261 GCAGGCCGGC GACGTGGAAG AGAATCCTGG CCCCAGCGAG CTGATCAAAG AGAACATGCA
     7321 CATGAAGCTG TACATGGAAG GCACCGTGGA CAACCATCAC TTCAAGTGCA CCAGCGAGGG
     7381 CGAGGGCAAG CCCTACGAGG GCACACAGAC CATGAGAATC AAGGTGGTGG AAGGAGGCCC
     7441 ACTGCCCTTC GCCTTCGACA TCCTGGCCAC CAGCTTTCTG TACGGCTCAA AGACCTTCAT
     7501 CAACCACACC CAGGGCATCC CTGATTTCTT CAAGCAGAGC TTCCCTGAGG GATTTACCTG
     7561 GGAGAGAGTG ACAACTTATG AAGATGGCGG AGTGCTGACC GCCACACAAG ATACCAGCCT
     7621 GCAGGACGGC TGTCTGATCT ACAACGTGAA GATCAGAGGC GTGAACTTCA CCAGCAACGG
     7681 CCCTGTGATG CAGAAAAAAA CCCTGGGCTG GGAGGCCTTT ACAGAGACAC TGTACCCCGC
     7741 TGATGGAGGC CTGGAAGGTA GAAACGACAT GGCCCTGAAA CTGGTCGGCG GCAGCCACCT
     7801 GATTGCCAAT ATCAAGACCA CATACCGGAG CAAGAAACCA GCTAAAAACC TGAAGATGCC
     7861 TGGCGTTTAC TACGTGGATT ACAGACTGGA GCGGATCAAG GAAGCCAACA ACGAGACATA
     7921 TGTGGAACAG CACGAGGTGG CCGTAGCTAG ATACTGCGAC CTGCCTTCTA AGCTTGGCCA
     7981 CAAGCTGAAC TGATAAgcgg ccgcactcct caggtgcagg ctgcctatca gaaggtggtg
     8041 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     8101 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     8161 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     8221 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     8281 atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc
     8341 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     8401 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     8461 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     8521 aagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8701 tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt
     8761 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8821 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8881 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     8941 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9001 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9061 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9481 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     9541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9721 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10681 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11041 cccgaaaagt gccacctggt c
//