LOCUS       Exported                6334 bp DNA     circular UNA 06-DEC-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 3, 2024 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     source          1333..2142
                     /mol_type="other DNA"
                     /label=source
                     /note="color: #ffffff"
                     /organism="pDisplay-BirA-ER"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     promoter        156..597
                     /label=synapsin
                     /note="color: #ffffff"
     primer_bind     444..456
                     /label=Primer 2
                     /note="color: black; sequence: 
                     cgctggctgTGCAACcccAGGccggccgggccgc; added: 2022-11-17"
     misc_feature    446..469
                     /label=ColonyF_hSyn
                     /note="color: #a6acb3"
     sig_peptide     598..660
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /note="color: #993366
                     Cleavage site after base 657"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     regulatory      598..601
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     protein_bind    661..685
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     663..687
                     /label=overlap-pdisplayJM IgK
                     /note="color: black; sequence: AAGTTTGTACAAAAAAGTTGGCACC"
     primer_bind     complement(665..687)
                     /label=R-pdisplayJM
                     /note="color: black; sequence: GGTGCCAACTTTTTTGTACAAAC"
     gene            688..2436
                     /label=ChichenP2Y6
                     /note="color: #ff99cc; direction: RIGHT"
     primer_bind     1295..1333
                     /label=Primer 1
                     /note="color: black; sequence: 
                     tggcctgctactgcagcatgGCCcgctggctgTGCAACc; added: 2022-11-17"
     misc_feature    1315..1317
                     /label=5.62
                     /note="color: #a6acb3"
     primer_bind     1318..1351
                     /label=Primer 2
                     /note="color: black; sequence: 
                     cgctggctgTGCAACcccAGGccggccgggccgc; added: 2022-11-17"
     misc_feature    1318..1320
                     /label=5.63
                     /note="color: #a6acb3"
     misc_feature    1321..1323
                     /label=5.64
                     /note="color: #ff0000"
     misc_feature    1324..1326
                     /label=5.65
                     /note="color: #a6acb3"
     misc_feature    1327..1329
                     /label=5.66
                     /note="color: #a6acb3"
     misc_feature    1330..1332
                     /label=5.67
                     /note="color: #ff0000"
     misc_feature    1333..2142
                     /label=NE loop
                     /note="color: #00ffff"
     misc_feature    1333..1335
                     /label=5.68
                     /note="color: #a6acb3"
     misc_feature    1336..1338
                     /label=A5.69K
                     /note="color: #a6acb3"
     misc_feature    1339..1341
                     /label=51
                     /note="color: #a6acb3"
     misc_feature    1342..1344
                     /label=52
                     /note="color: #a6acb3"
     misc_feature    1345..1347
                     /label=53
                     /note="color: #a6acb3"
     misc_feature    1348..1350
                     /label=54
                     /note="color: #a6acb3"
     misc_feature    1351..1353
                     /label=55
                     /note="color: #a6acb3"
     misc_feature    1354..1356
                     /label=56
                     /note="color: #a6acb3"
     misc_feature    1357..1359
                     /label=57
                     /note="color: #a6acb3"
     misc_feature    1360..1362
                     /label=58
                     /note="color: #a6acb3"
     misc_feature    1363..1365
                     /label=59
                     /note="color: #a6acb3"
     misc_feature    1366..1368
                     /label=60
                     /note="color: #a6acb3"
     misc_feature    1381..2124
                     /label=GG-cpEGFP-TGAAA
                     /note="color: #339966"
     primer_bind     1393..1444
                     /label=Ado1.1-cpEGFP-G12X-F
                     /note="Forward primer region"
                     /note="color: black; sequence: 
                     TATATCAAGGCCGACAAGCAGAAGAACGGCATCAAGGCGAACTTCCACATCC"
     primer_bind     complement(1400..1419)
                     /label=Ado1.1-cpEGFP-G12X-R
                     /note="Reverse primer region"
                     /note="color: black; sequence: GTTCTTCTGCTTGTCGGCCT"
     primer_bind     complement(1413..1458)
                     /label=N16X
                     /note="sequence:CTCGATGTTGTGGCGGATGTGGAAGTTCGCCTTGATGCCGTTC
                     TTC"
                     /note="color: black; sequence: 
                     ctcgatgttgtggcggatgtggaagttcgccttgatgccgttcttc"
     primer_bind     complement(1416..1469)
                     /label=H18X
                     /note="color: black; sequence: 
                     ACGCCGCCGTCCTCGATGTTGTGGCGGATGTGGAAGTTCGCCTTGATGCCGTTC"
     variation       1420..1422
                     /label=G12
                     /label=nonstandard type: mutation
                     /note="color: #a6acb3"
     variation       1432..1434
                     /label=N16
                     /label=nonstandard type: mutation
                     /note="color: #a6acb3"
     primer_bind     1434..1486
                     /label=Ado1.1-cpEGFP-D25X-F
                     /note="Forward primer region"
                     /note="color: black; sequence: 
                     CTTCCACATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCTACCACT"
     variation       1438..1440
                     /label=H18
                     /label=nonstandard type: mutation
                     /note="color: #a6acb3"
     primer_bind     complement(1439..1458)
                     /label=Ado1.1-cpEGFP-D25X-R
                     /note="Reverse primer region"
                     /note="color: black; sequence: CTCGATGTTGTGGCGGATGT"
     primer_bind     complement(1458..1510)
                     /label=Y32X
                     /note="color: black; sequence: 
                     CGCCGATGGGGGTGTTCTGCTGGTAGTGGTAGGCGAGCTGCACGCCGCCGTCC"
     variation       1459..1461
                     /label=D25
                     /label=nonstandard type: mutation
                     /note="color: #a6acb3"
     variation       1480..1482
                     /label=Y32
                     /label=nonstandard type: mutation
                     /note="color: #a6acb3"
     primer_bind     1593..1614
                     /label=pEGFP-C-5
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     complement(1638..1657)
                     /label=R-CAAX-gb-mcherry
                     /note="color: black; sequence: 
                     CACACTTTGTCTTTGACTTCTTTTTCTTCTTTTTACCATCCTTGTACAGCTCGTCCATG
                     "
     primer_bind     1674..1695
                     /label=F-IRES-NcoI-gb-mcherry
                     /note="color: black; sequence: 
                     AAAACACGATGATAATATGGCCACAACCATGGTGAGCAAGGGCGAGGAG"
     primer_bind     complement(1720..1741)
                     /label=GFP-R
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     1740..1791
                     /label=S127X
                     /note="color: black; sequence: 
                     CGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGTGAGGGCGATGCCACC"
     variation       1765..1767
                     /label=S127
                     /label=nonstandard type: mutation
                     /note="color: #a6acb3"
     misc_feature    1792..1794
                     /label=Y136
                     /note="color: #a6acb3"
     misc_feature    2083..2085
                     /label=5.67
                     /note="color: #a6acb3"
     misc_feature    2098..2100
                     /label=5.72
                     /note="color: #a6acb3"
     misc_feature    2128..2130
                     /label=6.28
                     /note="color: #a6acb3"
     misc_feature    2131..2133
                     /label=6.29
                     /note="color: #a6acb3"
     misc_feature    2134..2136
                     /label=G6.30H
                     /note="color: #a6acb3"
     misc_feature    2137..2139
                     /label=R6.31V
                     /note="color: #ff0000"
     misc_feature    2140..2142
                     /label=6.32
                     /note="color: #a6acb3"
     misc_feature    2143..2145
                     /label=6.33
                     /note="color: #999999"
     misc_feature    2146..2148
                     /label=6.34
                     /note="color: #a6acb3"
     misc_feature    2149..2151
                     /label=6.35
                     /note="color: #a6acb3"
     misc_feature    2152..2154
                     /label=6.36
                     /note="color: #a6acb3"
     primer_bind     complement(2417..2467)
                     /label=cP2Y6-WPRE-R
                     /note="color: black; sequence: 
                     GTTGATTATCGATAAGCTTGATATCGAATTCctaggagccgtagctgatgc; added:
                     2022-12-01"
     unsure          2451..3059
                     /label=WPRE
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    complement(2536..2553)
                     /label=pWPTRev
                     /note="color: #a6acb3; direction: LEFT"
     polyA_signal    3079..3557
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
                     /note="color: #a6acb3"
     unsure          3597..3737
                     /label=R-ITR
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     rep_origin      3829..4135
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
     unsure          4654..5511
                     /label=AmpR
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     rep_origin      5662..6329
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccagc atggccaact tcacgggggg gaggaactcg
      721 tgcaccttcc atgaggaatt caagcaggtc ctgctgcccc tggtctactc agtggtgttc
      781 ctactggggc tgccactcaa tgccgttgtc attgggcaga tctggctggc ccgcaaggcg
      841 ttgacccgca ccaccatcta catgctgaac ctggccatgg ccgacctgct ttatgtctgc
      901 tccctccctc tcctcatcta caactacacc cagaaggatt actggccctt tggggacttc
      961 acctgcaaat tcgtccgctt ccagttctac accaacctgc acggcagcat cctcttcctc
     1021 acctgcatca gcgtccagcg ctacatgggg atctgccacc ccttggcctc gtggcacaaa
     1081 aagaagggaa agaagctgac gtggctggtg tgtgctgccg tgtggttcat cgtcatcgcc
     1141 cagtgcctgc ccacctttgt cttcgcctcc accggcacgc agaggaatcg cactgtctgc
     1201 tatgacctga gccccccgga ccgctccaca tcctacttcc cctatggcat cacgttgacc
     1261 atcaccggct tcctgctgcc cttcgcagcc atcctggcct gctactgcag catggcccgc
     1321 tggctgtgca accccaagcc ggccgggccg cgcgacaccg acgcgctgga cctggaggag
     1381 ggaggaaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttccac
     1441 atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc
     1501 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt gcagtccaaa
     1561 ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1621 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg
     1681 agaaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1741 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag
     1801 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1861 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1921 gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag
     1981 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2041 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2101 gagtacaaca ccggagcagc agcacgctgg cgccacgtgc aggccgtgcg catgatcatc
     2161 atcgttgtca tcgtcttctc catcagcttc ttccccttcc acctcaccaa gaccatctac
     2221 ctgatcgtcc gctcctcagc cagcttgccc tgccctaccc tgcaggcttt tgccattgcc
     2281 tacaagtgca cgcggccctt tgccagcatg aacagcgtcc tcgaccccat cctcttctac
     2341 ttcacccagc gcaagtttcg tgagagcacc cgctatctcc tggacaagat gagctccaag
     2401 tggcggcaag accactgcat cagctacggc tcctaggaat tcgatatcaa gcttatcgat
     2461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2821 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2881 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3001 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     3061 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     3121 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     3181 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     3241 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     3301 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     3361 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     3421 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     3481 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     3541 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     3601 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     3661 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3721 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3781 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     3841 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3901 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3961 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4021 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4081 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4141 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4201 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4261 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4321 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4381 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4441 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4501 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4561 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4621 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4681 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4741 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4801 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4861 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4921 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4981 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5041 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5101 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5161 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5221 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5281 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5341 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5401 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5461 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5521 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5581 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5641 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5701 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5761 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5821 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5881 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5941 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6001 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6061 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6121 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6181 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6241 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6301 ttcctggcct tttgctggcc ttttgctcac atgt
//
