LOCUS       pHHM_iS-methadoneSnFR1. 4444 bp ds-DNA     circular     11-MAR-2025
DEFINITION  .
COMMENT     This map was received by Anand from Phil Borden (Looger Lab) in
            April 2018.
FEATURES             Location/Qualifiers
     primer          1195..1239
                     /label="fwd"
                     /note="sequence: ATGTTCTTTTTAGAAGGGCCCNNSGGTTACCCAGGCCTGCAAAAA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     DNA*_RANGE      1239..1238
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1258..1277
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1326..1361)
                     /label="bb-rev_gib"
                     /note="sequence: ACCATGATGATGATGATGATGCATATGTATATCTCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1338..1409
                     /label="Translation 1338-1409"
                     /translation="MHHHHHHGYPYDVPDYAGAQPARS"
     misc_feature    1341..1361
                     /label="His6Gly"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1362..1388
                     /label="HA Tag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     DNA*_cloneIn... 1362..2978
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1410..1425)
                     /label="bb-overlap-gib"
                     /note="sequence: CTACGGTGTCGTTCGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1410..1634
                     /label="X513OpuB 30-106"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1410..2972
                     /label="Translation 1410-2972"
                     /translation="ANDTVVVGSIVFTEGIIVANMVAEMIEAHTDLKVVRKLNLGGENVNFEAIKRGGANNGIDIYVEYTGHGLVDILGFPEPNVYITADKQKNGIKANFKIRHNMEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMSKGEELFTGVVPILVELDGGVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFPPPSSTDPEGAYETVKKEYKRKWNIVWLKPLGFNNTYTLTVKDELAKQYNLKTFSDLAKISDKLILGATMFFLEGPDGYPGLQKLYNFKFKHTKSMDMGIRYTAIDNNEVQVIDAFATDGLLVSHKLKILEDDKAFFPPYYAAPIIRQDVLDKHPELKDVLNKLANQISAEEMQKLNYKVDGEGQDPAKVAKEFLKEKGLI"
     primer          complement(1420..1442)
                     /label="12x-Rev"
                     /note="sequence: CACGATCGAGCCCACAACTACGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1430..1460
                     /label="12x-Fwd"
                     /note="sequence: GGGCTCGATCGTGTTTACAGAAGGGATTATC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1437..1439
                     /label="K39I"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1452..1454
                     /label="Q44G"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1536..1538
                     /label="T72E"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1569..1601)
                     /label="65x-Rev"
                     /note="sequence: CTCCACGTAAATGTCAATACCATTATTCGCACC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1574..1610)
                     /label="68x_rev"
                     /note="sequence: CCCAGTGTACTCCACGTAAATGTCAATACCATTATTC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1587..1613
                     /label="65x-Fwd_2"
                     /note="sequence: GACATTTACGTGGAGTACACTGGGCAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1599..1622)
                     /label="72x_rev"
                     /note="sequence: CACAAGACCGTGCCCAGTGTACTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1599..1623
                     /label="68x_fwd"
                     /note="sequence: GAGTACACTGGGCACGGTCTTGTGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1610..1637
                     /label="72x_fwd"
                     /note="sequence: GCACGGTCTTGTGGATATTCTGGGGTTC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1611..1613
                     /label="T97H"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1647..1916
                     /label="cpsfGFP 149-238"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1917..1934
                     /label="Linker"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1935..2369
                     /label="cpsfGFP 1-146"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2106..2126)
                     /label="SW-rev"
                     /note="sequence: CAGGGTGGTCACGAGGGTGGG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2108..2129)
                     /label="241x-rev"
                     /note="sequence: GGTCAGGGTGGTCACGAGGGTG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2119..2141
                     /label="SW-fwd"
                     /note="sequence: CCACCCTGACCTACGGCGTGCAG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2382..2384
                     /label="T108S"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2382..2972
                     /label="X513OpuB 107-305"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2397..2399
                     /label="K113G"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2430..2432
                     /label="D124R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2449..2477)
                     /label="357-Rev"
                     /note="sequence: CGTATTGTTGAATCCCAGTGGTTTGAGCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2460..2498
                     /label="357-Fwd"
                     /note="sequence: CTGGGATTCAACAATACGTATACGCTTACCGTTAAAGAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2487..2489
                     /label="A143T"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2552..2579)
                     /label="391x-Rev"
                     /note="sequence: CATCGTTGCACCCAGAATCAGCTTATCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2561..2594
                     /label="391x-Fwd"
                     /note="sequence: GATTCTGGGTGCAACGATGTTCTTTTTAGAAGGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2580..2582
                     /label="E174F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2592..2594
                     /label="K178G"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2622..2624
                     /label="V188L"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2679..2714)
                     /label="436-Rev"
                     /note="sequence: GGCATCAATTACCTGAACTTCGTTATTATCAATGGC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          2700..2728
                     /label="436-Fwd_2"
                     /note="sequence: CAGGTAATTGATGCCTTCGCCACTGATGG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2740..2777)
                     /label="457x-Rev"
                     /note="sequence: GAACGCTTTATCATCCTCCAGAATTTTTAATTTGTGGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2762..2786)
                     /label="460x-Rev"
                     /note="sequence: CGGCGGGAAGAACGCTTTATCATCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2763..2794
                     /label="457x-Fwd"
                     /note="sequence: GATGATAAAGCGTTCTTCCCGCCGTATTATGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2772..2774
                     /label="H238A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2777..2800
                     /label="460x-Fwd"
                     /note="sequence: CTTCCCGCCGTATTATGCTGCCCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2973..3015
                     /label="Translation 2973-3015"
                     /translation="LQVDEQKLISEEDL"
     misc_feature    2979..3020
                     /label="Myc Tag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             3015..3020
                     /label="Translation 3015-3020"
                     /translation="N*"
     misc_feature    complement(3084..3101)
                     /label="T7 Rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3241..3696
                     /label="f1 origin"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC
       61 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA
      121 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA
      181 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG
      241 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
      301 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
      361 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
      421 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
      481 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
      541 GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
      601 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
      661 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
      721 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
      781 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
      841 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
      901 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
      961 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     1021 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
     1081 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC
     1141 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA
     1201 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGGA TCTCGATCCC GCGAAATTAA
     1261 TACGACTCAC TATAGGGAGA CCACAACGGT TTCCCTCTAG AAATAATTTT GTTTAACTTT
     1321 AAGAAGGAGA TATACATATG CATCATCATC ATCATCATGG TTATCCCTAT GATGTTCCAG
     1381 ATTATGCTGG GGCCCAGCCG GCCAGATCTG CGAACGACAC CGTAGTTGTG GGCTCGATCG
     1441 TGTTTACAGA AGGGATTATC GTCGCAAACA TGGTGGCAGA GATGATTGAG GCGCATACAG
     1501 ACCTTAAGGT GGTTCGCAAA CTGAACCTTG GCGGGGAGAA CGTTAACTTT GAAGCCATTA
     1561 AACGCGGAGG TGCGAATAAT GGTATTGACA TTTACGTGGA GTACACTGGG CACGGTCTTG
     1621 TGGATATTCT GGGGTTCCCG GAGCCGAACG TCTATATCAC CGCCGACAAG CAGAAGAACG
     1681 GCATCAAGGC GAACTTCAAG ATCCGCCACA ACATGGAGGA CGGCAGCGTG CAGCTCGCCG
     1741 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
     1801 ACCTGAGCAC CCAGTCCGTG CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
     1861 TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGCG
     1921 GTACCGGAGG GAGCATGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG
     1981 TCGAGCTGGA CGGCGGCGTA AACGGCCACA AGTTCAGCGT GCGCGGCGAG GGCGAGGGCG
     2041 ATGCCACCAA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC
     2101 CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG
     2161 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC
     2221 GCACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG
     2281 GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA
     2341 TCCTGGGGCA CAAGCTGGAG TACAACTTTC CGCCGCCCAG CTCTACTGAT CCAGAAGGTG
     2401 CATACGAAAC CGTGAAGAAG GAGTACAAAC GTAAATGGAA TATTGTATGG CTCAAACCAC
     2461 TGGGATTCAA CAATACGTAT ACGCTTACCG TTAAAGACGA ACTGGCGAAA CAGTATAACC
     2521 TTAAAACCTT CAGTGACTTA GCGAAAATCT CGGATAAGCT GATTCTGGGT GCAACGATGT
     2581 TCTTTTTAGA AGGGCCCGAT GGTTACCCAG GCCTGCAAAA ACTGTACAAT TTCAAATTCA
     2641 AGCACACCAA AAGCATGGAC ATGGGTATTC GCTATACCGC CATTGATAAT AACGAAGTTC
     2701 AGGTAATTGA TGCCTTCGCC ACTGATGGCT TGCTGGTGAG CCACAAATTA AAAATTCTGG
     2761 AGGATGATAA AGCGTTCTTC CCGCCGTATT ATGCTGCCCC CATCATCCGT CAGGATGTCT
     2821 TAGATAAGCA TCCTGAACTG AAGGACGTGC TGAACAAACT CGCGAATCAA ATTTCAGCGG
     2881 AAGAAATGCA GAAACTGAAT TACAAGGTGG ACGGTGAGGG TCAGGACCCA GCGAAAGTAG
     2941 CTAAGGAGTT TTTGAAAGAG AAAGGTTTAA TTctgcagGT CGACGAACAA AAACTCATCT
     3001 CAGAAGAGGA TCTGAATTAA GAATTCGAAG CTTGATCCGG CTGCTAACAA AGCCCGAAAG
     3061 GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     3121 AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATC TGGCGTAATA
     3181 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG
     3241 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
     3301 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
     3361 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA
     3421 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC
     3481 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG
     3541 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT
     3601 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA
     3661 ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT
     3721 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
     3781 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA
     3841 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC
     3901 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT
     3961 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC
     4021 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG
     4081 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC
     4141 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT
     4201 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA
     4261 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC
     4321 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC
     4381 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT
     4441 AATA
//