LOCUS       pTwist-CMV-iSmethadoneS 6135 bp ds-DNA     circular     30-JUN-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..63
                     /label="igkappa leader"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1..69
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="METDTLLLWVLLLWVPGSTGDRS"
     misc_feature    70..312
                     /label="GAT tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             70..333
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="RLREPLLSGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGRDLSRLPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGAKDPPVAT"
     misc_feature    313..333
                     /label="linker"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    334..558
                     /label="X513OpuB 30-106"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             334..1896
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="ANDTVVVGSIVFTEGIIVANMVAEMIEAHTDLKVVRKLNLGGENVNFEAIKRGGANNGIDIYVEYTGHGLVDILGFPEPNVYITADKQKNGIKANFKIRHNMEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMSKGEELFTGVVPILVELDGGVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFPPPSSTDPEGAYETVKKEYKRKWNIVWLKPLGFNNTYTLTVKDELAKQYNLKTFSDLAKISDKLILGATMFFLEGPDGYPGLQKLYNFKFKHTKSMDMGIRYTAIDNNEVQVIDAFATDGLLVSHKLKILEDDKAFFPPYYAAPIIRQDVLDKHPELKDVLNKLANQISAEEMQKLNYKVDGEGQDPAKVAKEFLKEKGLI"
     misc_feature    559..570
                     /label="N-term linker"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    571..840
                     /label="cpGFP 149-238"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    841..858
                     /label="linker in GFP"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    859..1293
                     /label="cpGFP 1-146"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1294..1305
                     /label="C-term linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1306..1896
                     /label="X513OpuB 107-305"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             1897..1944
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="LQVDEQKLISEEDLN*"
     misc_feature    1909..1938
                     /label="Myc tag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1994..2115
                     /label="SV40"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2784..3372
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4118..4978
                     /label="AmpR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    5853..6055
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 ATGGAGACAG ACACACTCCT GCTATGGGTA CTGCTGCTCT GGGTTCCAGG TTCCACTGGT
       61 GACAGATCTA GGCTTCGGGA GCCGCTCCTG AGCGGCAGCG CCGCGATGCC AGGCGCGTCC
      121 CTACAGCGGG CCTGCCGCCT GCTCGTGGCC GTCTGCGCTC TGCACCTTGG CGTCACCCTC
      181 GTTTACTACC TGGCTGGCCG CGACCTGAGC CGCCTGCCCC AACTGGTCGG AGTCTCCACA
      241 CCGCTGCAGG GCGGCTCGAA CAGTGCCGCC GCCATCGGGC AGTCCTCCGG GGAGCTCCGG
      301 ACCGGAGGGG CCAAGGATCC ACCGGTCGCC ACCGCGAACG ACACCGTAGT TGTGGGCTCG
      361 ATCGTGTTTA CAGAAGGGAT TATCGTCGCA AACATGGTGG CAGAGATGAT TGAGGCGCAT
      421 ACAGACCTTA AGGTGGTTCG CAAACTGAAC CTTGGCGGGG AGAACGTTAA CTTTGAAGCC
      481 ATTAAACGCG GAGGTGCGAA TAATGGTATT GACATTTACG TGGAGTACAC TGGGCACGGT
      541 CTTGTGGATA TTCTGGGGTT CCCGGAGCCG AACGTCTATA TCACCGCCGA CAAGCAGAAG
      601 AACGGCATCA AGGCGAACTT CAAGATCCGC CACAACATGG AGGACGGCAG CGTGCAGCTC
      661 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC
      721 CACTACCTGA GCACCCAGTC CGTGCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG
      781 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG
      841 GGCGGTACCG GAGGGAGCAT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
      901 CTGGTCGAGC TGGACGGCGG CGTAAACGGC CACAAGTTCA GCGTGCGCGG CGAGGGCGAG
      961 GGCGATGCCA CCAACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
     1021 GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC
     1081 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
     1141 GAGCGCACCA TCAGCTTCAA GGACGACGGC ACCTACAAGA CCCGCGCCGA GGTGAAGTTC
     1201 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
     1261 AACATCCTGG GGCACAAGCT GGAGTACAAC TTTCCGCCGC CCAGCTCTAC TGATCCAGAA
     1321 GGTGCATACG AAACCGTGAA GAAGGAGTAC AAACGTAAAT GGAATATTGT ATGGCTCAAA
     1381 CCACTGGGAT TCAACAATAC GTATACGCTT ACCGTTAAAG ACGAACTGGC GAAACAGTAT
     1441 AACCTTAAAA CCTTCAGTGA CTTAGCGAAA ATCTCGGATA AGCTGATTCT GGGTGCAACG
     1501 ATGTTCTTTT TAGAAGGGCC CGATGGTTAC CCAGGCCTGC AAAAACTGTA CAATTTCAAA
     1561 TTCAAGCACA CCAAAAGCAT GGACATGGGT ATTCGCTATA CCGCCATTGA TAATAACGAA
     1621 GTTCAGGTAA TTGATGCCTT CGCCACTGAT GGCTTGCTGG TGAGCCACAA ATTAAAAATT
     1681 CTGGAGGATG ATAAAGCGTT CTTCCCGCCG TATTATGCTG CCCCCATCAT CCGTCAGGAT
     1741 GTCTTAGATA AGCATCCTGA ACTGAAGGAC GTGCTGAACA AACTCGCGAA TCAAATTTCA
     1801 GCGGAAGAAA TGCAGAAACT GAATTACAAG GTGGACGGTG AGGGTCAGGA CCCAGCGAAA
     1861 GTAGCTAAGG AGTTTTTGAA AGAGAAAGGT TTAATTCTGC AGGTCGACGA ACAAAAACTC
     1921 ATCTCAGAAG AGGATCTGAA TTAGGCTAGC TTGACTGACT GAGATACAGC GTACCTTCAG
     1981 CTCACAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA
     2041 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
     2101 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
     2161 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATTGG CCCATCTCTA
     2221 TCGGTATCGT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGTGCC
     2281 CCTCGGGCCG GATTGCTATC TACCGGCATT GGCGCAGAAA AAAATGCCTG ATGCGACGCT
     2341 GCGCGTCTTA TACTCCCACA TATGCCAGAT TCAGCAACGG ATACGGCTTC CCCAACTTGC
     2401 CCACTTCCAT ACGTGTCCTC CTTACCAGAA ATTTATCCTT AAGGTCGTCA GCTATCCTGC
     2461 AGGCGATCTC TCGATTTCGA TCAAGACATT CCTTTAATGG TCTTTTCTGG ACACCACTAG
     2521 GGGTCAGAAG TAGTTCATCA AACTTTCTTC CCTCCCTAAT CTCATTGGTT ACCTTGGGCT
     2581 ATCGAAACTT AATTAACCAG TCAAGTCAGC TACTTGGCGA GATCGACTTG TCTGGGTTTC
     2641 GACTACGCTC AGAATTGCGT CAGTCAAGTT CGATCTGGTC CTTGCTATTG CACCCGTTCT
     2701 CCGATTACGA GTTTCATTTA AATCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
     2761 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
     2821 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
     2881 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
     2941 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
     3001 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
     3061 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
     3121 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
     3181 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
     3241 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
     3301 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
     3361 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     3421 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     3481 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     3541 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     3601 ATAGTTGCAT TTAAATTTCC GAACTCTCCA AGGCCCTCGT CGGAAAATCT TCAAACCTTT
     3661 CGTCCGATCC ATCTTGCAGG CTACCTCTCG AACGAACTAT CGCAAGTCTC TTGGCCGGCC
     3721 TTGCGCCTTG GCTATTGCTT GGCAGCGCCT ATCGCCAGGT ATTACTCCAA TCCCGAATAT
     3781 CCGAGATCGG GATCACCCGA GAGAAGTTCA ACCTACATCC TCAATCCCGA TCTATCCGAG
     3841 ATCCGAGGAA TATCGAAATC GGGGCGCGCC TGGTGTACCG AGAACGATCC TCTCAGTGCG
     3901 AGTCTCGACG ATCCATATCG TTGCTTGGCA GTCAGCCAGT CGGAATCCAG CTTGGGACCC
     3961 AGGAAGTCCA ATCGTCAGAT ATTGTACTCA AGCCTGGTCA CGGCAGCGTA CCGATCTGTT
     4021 TAAACCTAGA TATTGATAGT CTGATCGGTC AACGTATAAT CGAGTCCTAG CTTTTGCAAA
     4081 CATCTATCAA GAGACAGGAT CAGCAGGAGG CTTTCGCATG AGTATTCAAC ATTTCCGTGT
     4141 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
     4201 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCGCGA GTGGGTTACA TCGAACTGGA
     4261 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGCTTTC CAATGATGAG
     4321 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
     4381 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTATTCAC CAGTCACAGA
     4441 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
     4501 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATTGGA GGACCGAAGG AGCTAACCGC
     4561 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
     4621 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACCTT
     4681 GCGTAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAGT TGATAGACTG
     4741 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
     4801 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG
     4861 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT
     4921 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACC
     4981 GATTCTAGGT GCATTGGCGC AGAAAAAAAT GCCTGATGCG ACGCTGCGCG TCTTATACTC
     5041 CCACATATGC CAGATTCAGC AACGGATACG GCTTCCCCAA CTTGCCCACT TCCATACGTG
     5101 TCCTCCTTAC CAGAAATTTA TCCTTAAGAT CCCGAATCGT TTAAACTCGA CTCTGGCTCT
     5161 ATCGAATCTC CGTCGTTTCG AGCTTACGCG AACAGCCGTG GCGCTCATTT GCTCGTCGGG
     5221 CATCGAATCT CGTCAGCTAT CGTCAGCTTA CCTTTTTGGC AGCGATCGCG GCTCCCGACA
     5281 TCTTGGACCA TTAGCTCCAC AGGTATCTTC TTCCCTCTAG TGGTCATAAC AGCAGCTTCA
     5341 GCTACCTCTC AATTCAAAAA ACCCCTCAAG ACCCGTTTAG AGGCCCCAAG GGGTTATGCT
     5401 ATCAATCGTT GCGTTACACA CACAAAAAAC CAACACACAT CCATCTTCGA TGGATAGCGA
     5461 TTTTATTATC TAACTGCTGA TCGAGTGTAG CCAGATCTAG TAATCAATTA CGGGGTCATT
     5521 AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG
     5581 CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC
     5641 GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT
     5701 GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA
     5761 ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA
     5821 CATCTACGTA TTAGTCATCG CTATTACCAT GCTGATGCGG TTTTGGCAGT ACATCAATGG
     5881 GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG
     5941 GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC
     6001 ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT
     6061 AGTGAACCGT CAGATCAGAT CTTTGTCGAT CCTACCATCC ACTCGACACA CCCGCCAGCG
     6121 GCCGCTGCCA AGCTT
//