LOCUS       hSyn_HK1       11121 bp DNA     circular SYN 06-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
FEATURES             Location/Qualifiers
     source          1..11121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    3046..3051
                     /label=Kozak
     CDS             3052..5802
                     /codon_start=1
                     /label=hHK1
                     /translation="MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRK
                     EMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQN
                     VHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKI
                     DEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQ
                     HCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREI
                     DRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVS
                     AIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRD
                     NKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTA
                     VAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSFVR
                     RTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEIMQGTGEELFD
                     HIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVT
                     LLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVE
                     MVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEI
                     VRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNST
                     CDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHF
                     SRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTEASS"
     primer_bind     complement(5547..5558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    5927..6515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5980..6000)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6398..6409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6922..7102
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7128..7145)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7134..7358
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7404..7832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7491..7510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7701..7722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7841..7861)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7846..8176
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8027..8162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8089..8108
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8224..8271
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8290..8664
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8794..8915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8831..8850)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8885..8904
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8964..8980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8964..8980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8977..8999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8988..9004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9012..9018,9019..9036,9037..9042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9057..9078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9195..9212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9366..9954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9446..9465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10125..10916,10917..10985))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10748..10767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10986..11090)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgaggccac catgatcgcc
     3061 gcgcagctcc tggcctatta cttcacggag ctgaaggatg accaggtcaa aaagattgac
     3121 aagtatctgt atgccatgcg gctctccgat gaaactctca tagatatcat gactcgcttc
     3181 aggaaggaga tgaagaatgg cctctcccgg gattttaatc caacagccac agtcaagatg
     3241 ttgccaacat tcgtaaggtc cattcctgat ggctctgaaa agggagattt cattgccctg
     3301 gatcttggtg ggtcttcctt tcgaattctg cgggtgcaag tgaatcatga gaaaaaccag
     3361 aatgttcaca tggagtccga ggtttatgac accccagaga acatcgtgca cggcagtgga
     3421 agccagcttt ttgatcatgt tgctgagtgc ctgggagatt tcatggagaa aaggaagatc
     3481 aaggacaaga agttacctgt gggattcacg ttttcttttc cttgccaaca atccaaaata
     3541 gatgaggcca tcctgatcac ctggacaaag cgatttaaag cgagcggagt ggaaggagca
     3601 gatgtggtca aactgcttaa caaagccatc aaaaagcgag gggactatga tgccaacatc
     3661 gtagctgtgg tgaatgacac agtgggcacc atgatgacct gtggctatga cgaccagcac
     3721 tgtgaagtcg gcctgatcat cggcactggc accaatgctt gctacatgga ggaactgagg
     3781 cacattgatc tggtggaagg agacgagggg aggatgtgta tcaatacaga atggggagcc
     3841 tttggagacg atggatcatt agaagacatc cggacagagt ttgacaggga gatagaccgg
     3901 ggatccctca accctggaaa acagctgttt gagaagatgg tcagtggcat gtacttggga
     3961 gagctggttc gactgatcct agtcaagatg gccaaggagg gcctcttatt tgaagggcgg
     4021 atcaccccgg agctgctcac ccgagggaag tttaacacca gtgatgtgtc agccatcgaa
     4081 aagaataagg aaggcctcca caatgccaaa gaaatcctga cccgcctggg agtggagccg
     4141 tccgatgatg actgtgtctc agtccagcac gtttgcacca ttgtctcatt tcgctcagcc
     4201 aacttggtgg ctgccacact gggcgccatc ttgaaccgcc tgcgtgataa caagggcaca
     4261 cccaggctgc ggaccacggt tggtgtcgac ggatctcttt acaagacgca cccacagtat
     4321 tcccggcgtt tccacaagac tctaaggcgc ttggtgccag actccgatgt gcgcttcctc
     4381 ctctcggaga gtggcagcgg caagggggct gccatggtga cggcggtggc ctaccgcttg
     4441 gccgagcagc accggcagat agaggagacc ctggctcatt tccacctcac caaagacatg
     4501 ctgctggagg tgaagaagag gatgcgggcc gagatggagc tggggctgag gaagcagacg
     4561 cacaacaatg ccgtggttaa gatgctgccc tccttcgtcc ggagaactcc cgacgggacc
     4621 gagaatggtg acttcttggc cctggatctt ggaggaacca atttccgtgt gctgctggtg
     4681 aaaatccgta gtgggaaaaa gagaacggtg gaaatgcaca acaagatcta cgccattcct
     4741 attgaaatca tgcagggcac tggggaagag ctgtttgatc acattgtctc ctgcatctct
     4801 gacttcttgg actacatggg gatcaaaggc cccaggatgc ctctgggctt cacgttctca
     4861 tttccctgcc agcagacgag tctggacgcg ggaatcttga tcacgtggac aaagggtttt
     4921 aaggcaacag actgcgtggg ccacgatgta gtcaccttac taagggatgc gataaaaagg
     4981 agagaggaat ttgacctgga cgtggtggct gtggtcaacg acacagtggg caccatgatg
     5041 acctgtgctt atgaggagcc cacctgtgag gttggactca ttgttgggac cggcagcaat
     5101 gcctgctaca tggaggagat gaagaacgtg gagatggtgg agggggacca ggggcagatg
     5161 tgcatcaaca tggagtgggg ggcctttggg gacaacgggt gtctggatga tatcaggaca
     5221 cactacgaca gactggtgga cgaatattcc ctaaatgctg ggaaacaaag gtatgagaag
     5281 atgatcagtg gtatgtacct gggtgaaatc gtccgcaaca tcttaatcga cttcaccaag
     5341 aagggattcc tcttccgagg gcagatctct gagacgctga agacccgggg catctttgag
     5401 accaagtttc tctctcagat cgagagtgac cgattagcac tgctccaggt ccgggctatc
     5461 ctccagcagc taggtctgaa tagcacctgc gatgacagta tcctcgtcaa gacagtgtgc
     5521 ggggtggtgt ccaggagggc cgcacagctg tgtggcgcag gcatggctgc ggttgtggat
     5581 aagatccgcg agaacagagg actggaccgt ctgaatgtga ctgtgggagt ggacgggaca
     5641 ctctacaagc ttcatccaca cttctccaga atcatgcacc agacggtgaa ggaactgtca
     5701 ccaaaatgta acgtgtcctt cctcctgtct gaggatggca gcggcaaggg ggccgccctc
     5761 atcacggccg tgggcgtgcg gttacgcaca gaggcaagca gctaagttta aacctggaaa
     5821 aacatggagc aatcacaagt agcaacacag cagctaccaa tgctgcttgt gcctggctag
     5881 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcagacaatc aacctctgga
     5941 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6001 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6061 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6121 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6181 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6241 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6301 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6361 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6421 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6481 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta
     6541 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6601 ctggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     6661 acacaaggct acttccctga ttgacagaac tacacaccag ggccaggggt cagatatcca
     6721 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataagat agaagaggcc
     6781 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     6841 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     6901 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6961 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7021 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7081 tcagtgtgga aaatctctag cagggccgct ttaaacccgc tgatcagcct cgactgtgcc
     7141 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7201 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7261 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7321 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7381 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7441 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7501 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7561 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7621 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7681 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7741 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
     7801 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7861 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta
     7921 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7981 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8041 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8101 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8161 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     8221 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     8281 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     8341 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     8401 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     8461 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     8521 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     8581 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     8641 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     8701 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8761 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8821 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8881 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     8941 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9001 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9061 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9121 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9181 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9241 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9301 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9541 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9781 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9901 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9961 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10021 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10081 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10141 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10201 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10261 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10321 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10381 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10441 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10501 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10561 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10621 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10681 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10741 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10801 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10861 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10921 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10981 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11041 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11101 cgaaaagtgc cacctgacgt c
//
