LOCUS       hSyn_AldoA        9527 bp DNA     circular SYN 06-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    3067..3072
                     /label=Kozak
     CDS             3073..4167
                     /codon_start=1
                     /label=hAldoA
                     /translation="MPYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQ
                     SIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGV
                     VGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALA
                     IMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHI
                     YLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEAS
                     INLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQ
                     GKYTPSGQAGAAASESLFVSNHAY"
     misc_feature    4182..4192
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     misc_feature    4333..4921
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4386..4406)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4804..4815)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5328..5508
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5534..5551)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5540..5764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5810..6238
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5897..5916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6107..6128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6247..6267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6252..6582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6433..6568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6495..6514
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6630..6677
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6696..7070
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7200..7321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7237..7256)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7291..7310
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7370..7386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7370..7386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7383..7405)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7394..7410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7418..7424,7425..7442,7443..7448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7463..7484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7601..7618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7772..8360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7852..7871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8531..9322,9323..9391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9154..9173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9392..9496)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagagatc cgctagcgct
     3061 accggtgcca ccatgcccta ccaatatcca gcactgaccc cggagcagaa gaaggagctg
     3121 tctgacatcg ctcaccgcat cgtggcacct ggcaagggca tcctggctgc agatgagtcc
     3181 actgggagca ttgccaagcg gctgcagtcc attggcaccg agaacaccga ggagaaccgg
     3241 cgcttctacc gccagctgct gctgacagct gacgaccgcg tgaacccctg cattgggggt
     3301 gtcatcctct tccatgagac actctaccag aaggcggatg atgggcgtcc cttcccccaa
     3361 gttatcaaat ccaagggcgg tgttgtgggc atcaaggtag acaagggcgt ggtccccctg
     3421 gcagggacaa atggcgagac taccacccaa gggttggatg ggctgtctga gcgctgtgcc
     3481 cagtacaaga aggacggagc tgacttcgcc aagtggcgtt gtgtgctgaa gattggggaa
     3541 cacaccccct cagccctcgc catcatggaa aatgccaatg ttctggcccg ttatgccagt
     3601 atctgccagc agaatggcat tgtgcccatc gtggagcctg agatcctccc tgatggggac
     3661 catgacttga agcgctgcca gtatgtgacc gagaaggtgc tggctgctgt ctacaaggct
     3721 ctgagtgacc accacatcta cctggaaggc accttgctga agcccaacat ggtcacccca
     3781 ggccatgctt gcactcagaa gttttctcat gaggagattg ccatggcgac cgtcacagcg
     3841 ctgcgccgca cagtgccccc cgctgtcact gggatcacct tcctgtctgg aggccagagt
     3901 gaggaggagg cgtccatcaa cctcaatgcc attaacaagt gccccctgct gaagccctgg
     3961 gccctgacct tctcctacgg ccgagccctg caggcctctg ccctgaaggc ctggggcggg
     4021 aagaaggaga acctgaaggc tgcgcaggag gagtatgtca agcgagccct ggccaacagc
     4081 cttgcctgtc aaggaaagta cactccgagc ggtcaggctg gggctgctgc cagcgagtcc
     4141 ctcttcgtct ctaaccacgc ctattaagga tccaccggat ctagataact gatcataatc
     4201 agccatacca cgtttaaacc tggaaaaaca tggagcaatc acaagtagca acacagcagc
     4261 taccaatgct gcttgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     4321 cacacctcag acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4381 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4441 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4501 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4561 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4621 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4681 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     4741 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4801 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4861 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4921 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     4981 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa agaagacaag
     5041 atatccttga tctgtggatc taccacacac aaggctactt ccctgattga cagaactaca
     5101 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     5161 ttgagccaga taagatagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     5221 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     5281 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     5341 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5401 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5461 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gccgctttaa
     5521 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5581 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5641 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5701 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5761 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5821 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5881 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5941 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg
     6001 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6061 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6121 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6181 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     6241 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga
     6301 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6361 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6421 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6481 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     6541 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     6601 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     6661 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     6721 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     6781 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     6841 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     6901 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     6961 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     7021 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     7081 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7141 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7201 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7261 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7321 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7381 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7441 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7501 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7561 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7621 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     7981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8401 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8581 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8821 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8941 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9121 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9481 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
