LOCUS       hSyn_AldoC        9560 bp DNA     circular SYN 06-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
FEATURES             Location/Qualifiers
     source          1..9560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3106..4200
                     /codon_start=1
                     /label=hAldoC
                     /translation="MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLS
                     QIGVENTEENRRLYRQVLFSADDRVKKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGIV
                     VGIKVDKGVVPLAGTDGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALA
                     ILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHV
                     YLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEAS
                     FNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAGAATEEFIKRAEVNGLAAQ
                     GKYEGSGEDGGAAAQSLYIANHAY"
     misc_feature    4215..4225
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     misc_feature    4366..4954
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4419..4439)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4837..4848)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5361..5541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5567..5584)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5573..5797
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5843..6271
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5930..5949)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6140..6161
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6280..6300)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6285..6615
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6466..6601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6528..6547
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6663..6710
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6729..7103
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7233..7354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7270..7289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7324..7343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7403..7419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7403..7419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7416..7438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7427..7443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7451..7457,7458..7475,7476..7481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7496..7517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7634..7651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7805..8393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7885..7904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8564..9355,9356..9424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9187..9206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9425..9529)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagagatc cgctagcgct
     3061 accggactca gatctcgagc tcaagcttcg aattctgcag tcgacatgcc tcactcgtac
     3121 ccagcccttt ctgctgagca gaagaaggag ttgtctgaca ttgccctgcg gattgtagcc
     3181 ccgggcaaag gcattctggc tgcggatgag tctgtaggca gcatggccaa gcggctgagc
     3241 caaattgggg tggaaaacac agaggagaac cgccggctgt accgccaggt cctgttcagt
     3301 gctgatgacc gtgtgaaaaa gtgcattgga ggcgtcattt tcttccatga gaccctctac
     3361 cagaaagatg ataatggtgt tcccttcgtc cgaaccatcc aggataaggg catcgtcgtg
     3421 ggcatcaagg ttgacaaggg tgtggtgcct ctagctggga ctgatggaga aaccaccact
     3481 caagggctgg atgggctctc agaacgctgt gcccaataca agaaggatgg tgctgacttt
     3541 gccaagtggc gctgtgtgct gaaaatcagt gagcgtacac cctctgcact tgccattctg
     3601 gagaacgcca acgtgctggc ccgttatgcc agtatctgcc agcagaatgg cattgtgcct
     3661 attgtggaac ctgaaatatt gcctgatgga gaccacgacc tcaaacgttg tcagtatgtt
     3721 acagagaagg tcttggctgc tgtgtacaag gccctgagtg accatcatgt atacctggag
     3781 gggaccctgc tcaagcccaa catggtgacc ccgggccatg cctgtcccat caagtatacc
     3841 ccagaggaga ttgccatggc aactgtcact gccctgcgtc gcactgtgcc cccagctgtc
     3901 ccaggagtga ccttcctgtc tgggggtcag agcgaagaag aggcatcatt caacctcaat
     3961 gccatcaacc gctgccccct tccccgaccc tgggcgctta ccttctccta tgggcgtgcc
     4021 ctgcaagcct ctgcactcaa tgcctggcga gggcaacggg acaatgctgg ggctgccact
     4081 gaggagttca tcaagcgggc tgaggtgaat gggcttgcag cccagggcaa gtatgaaggc
     4141 agtggagaag atggtggagc agcagcacag tcactctaca ttgccaacca tgcctactga
     4201 ggatccaccg gatctagata actgatcata atcagccata ccacgtttaa acctggaaaa
     4261 acatggagca atcacaagta gcaacacagc agctaccaat gctgcttgtg cctggctaga
     4321 agcacaagag gaggaggagg tgggttttcc agtcacacct cagacaatca acctctggat
     4381 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4441 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4501 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4561 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4621 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4681 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4741 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     4801 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4861 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4921 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa
     4981 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5041 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     5101 cacaaggcta cttccctgat tgacagaact acacaccagg gccaggggtc agatatccac
     5161 tgacctttgg atggtgctac aagctagtac cagttgagcc agataagata gaagaggcca
     5221 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     5281 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     5341 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5401 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5461 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5521 cagtgtggaa aatctctagc agggccgctt taaacccgct gatcagcctc gactgtgcct
     5581 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5641 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5701 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5761 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5821 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5881 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5941 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     6001 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6061 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6121 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6181 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     6241 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     6301 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     6361 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6421 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6481 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6541 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6601 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     6661 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     6721 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     6781 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     6841 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     6901 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     6961 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     7021 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     7081 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     7141 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7201 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     7261 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7321 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     7381 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7441 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7501 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7561 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7981 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     8041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8221 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9541 gaaaagtgcc acctgacgtc
//
