LOCUS       hSyn_GAPDH        9470 bp DNA     circular SYN 06-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    3066..3095
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     CDS             3103..4110
                     /codon_start=1
                     /label=hGAPDH
                     /translation="MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVY
                     MFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTT
                     MEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKV
                     IHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIP
                     ELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQV
                     VSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE"
     misc_feature    4125..4135
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     misc_feature    4276..4864
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4329..4349)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4747..4758)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5271..5451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5477..5494)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5483..5707
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5753..6181
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5840..5859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6050..6071
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6190..6210)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6195..6525
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6376..6511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6438..6457
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6573..6620
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6639..7013
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7143..7264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7180..7199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7234..7253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7313..7329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7313..7329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7326..7348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7337..7353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7361..7367,7368..7385,7386..7391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7406..7427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7544..7561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7715..8303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7795..7814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8474..9265,9266..9334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9097..9116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9335..9439)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagagatc cgctagcgct
     3061 accggactca gatctcgagc tcaagcttcg aattcgctcg agatggggaa ggtgaaggtc
     3121 ggagtcaacg gatttggtcg tattgggcgc ctggtcacca gggctgcttt taactctggt
     3181 aaagtggata ttgttgccat caatgacccc ttcattgacc tcaactacat ggtttacatg
     3241 ttccaatatg attccaccca tggcaaattc catggcaccg tcaaggctga gaacgggaag
     3301 cttgtcatca atggaaatcc catcaccatc ttccaggagc gagatccctc caaaatcaag
     3361 tggggcgatg ctggcgctga gtacgtcgtg gagtccactg gcgtcttcac caccatggag
     3421 aaggctgggg ctcatttgca ggggggagcc aaaagggtca tcatctctgc cccctctgct
     3481 gatgccccca tgttcgtcat gggtgtgaac catgagaagt atgacaacag cctcaagatc
     3541 atcagcaatg cctcctgcac caccaactgc ttagcacccc tggccaaggt catccatgac
     3601 aactttggta tcgtggaagg actcatgacc acagtccatg ccatcactgc cacccagaag
     3661 actgtggatg gcccctccgg gaaactgtgg cgtgatggcc gcggggctct ccagaacatc
     3721 atccctgcct ctactggcgc tgccaaggct gtgggcaagg tcatccctga gctgaacggg
     3781 aagctcactg gcatggcctt ccgtgtcccc actgccaacg tgtcagtggt ggacctgacc
     3841 tgccgtctag aaaaacctgc caaatatgat gacatcaaga aggtggtgaa gcaggcgtcg
     3901 gagggccccc tcaagggcat cctgggctac actgagcacc aggtggtctc ctctgacttc
     3961 aacagcgaca cccactcctc cacctttgac gctggggctg gcattgccct caacgaccac
     4021 tttgtcaagc tcatttcctg gtatgacaac gaatttggct acagcaacag ggtggtggac
     4081 ctcatggccc acatggcctc caaggagtaa ggatccaccg gatctagata actgatcata
     4141 atcagccata ccacgtttaa acctggaaaa acatggagca atcacaagta gcaacacagc
     4201 agctaccaat gctgcttgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     4261 agtcacacct cagacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4321 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4381 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4441 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4501 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4561 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4621 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     4681 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4741 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4801 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4861 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4921 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac
     4981 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tgacagaact
     5041 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     5101 cagttgagcc agataagata gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     5161 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     5221 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggactgtact gggtctctct
     5281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggccgctt
     5461 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5521 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5581 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5641 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5701 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5761 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5821 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5881 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
     5941 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6001 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6061 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6121 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6181 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc
     6241 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     6481 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6541 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     6601 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     6661 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     6721 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     6781 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     6841 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     6901 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     6961 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     7021 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7081 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7141 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7201 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7261 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7321 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7381 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7441 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7501 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     7921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8341 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8401 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8461 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8521 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8581 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8641 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8701 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8761 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8821 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8881 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8941 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9001 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9061 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9121 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9181 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9241 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9301 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9361 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9421 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
