LOCUS       hSyn_PGK1        9725 bp DNA     circular SYN 06-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
FEATURES             Location/Qualifiers
     source          1..9725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    3062..3112
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     CDS             3112..4362
                     /codon_start=1
                     /label=hPGK1
                     /translation="MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVP
                     SIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEV
                     EKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVN
                     DAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQ
                     LINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKIT
                     LPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPV
                     GVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGAS
                     LELLEGKVLPGVDALSNI"
     misc_feature    4380..4390
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     misc_feature    4531..5119
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4584..4604)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5002..5013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5526..5706
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5732..5749)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5738..5962
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6008..6436
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6095..6114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6305..6326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6445..6465)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6450..6780
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6631..6766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6693..6712
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6828..6875
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6894..7268
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7398..7519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7435..7454)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7489..7508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7568..7584)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7568..7584)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7581..7603)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7592..7608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7616..7622,7623..7640,7641..7646))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7661..7682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7799..7816)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7970..8558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8050..8069)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8729..9520,9521..9589))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9352..9371
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9590..9694)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagagatc cgctagcgct
     3061 accggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac catgtcgctt
     3121 tctaacaagc tgacgctgga caagctggac gttaaaggga agcgggtcgt tatgagagtc
     3181 gacttcaatg ttcctatgaa gaacaaccag ataacaaaca accagaggat taaggctgct
     3241 gtcccaagca tcaaattctg cttggacaat ggagccaagt cggtagtcct tatgagccac
     3301 ctaggccggc ctgatggtgt gcccatgcct gacaagtact ccttagagcc agttgctgta
     3361 gaactcaaat ctctgctggg caaggatgtt ctgttcttga aggactgtgt aggcccagaa
     3421 gtggagaaag cctgtgccaa cccagctgct gggtctgtca tcctgctgga gaacctccgc
     3481 tttcatgtgg aggaagaagg gaagggaaaa gatgcttctg ggaacaaggt taaagccgag
     3541 ccagccaaaa tagaagcttt ccgagcttca ctttccaagc taggggatgt ctatgtcaat
     3601 gatgcttttg gcactgctca cagagcccac agctccatgg taggagtcaa tctgccacag
     3661 aaggctggtg ggtttttgat gaagaaggag ctgaactact ttgcaaaggc cttggagagc
     3721 ccagagcgac ccttcctggc catcctgggc ggagctaaag ttgcagacaa gatccagctc
     3781 atcaataata tgctggacaa agtcaatgag atgattattg gtggtggaat ggcttttacc
     3841 ttccttaagg tgctcaacaa catggagatt ggcacttctc tgtttgatga agagggagcc
     3901 aagattgtca aagacctaat gtccaaagct gagaagaatg gtgtgaagat taccttgcct
     3961 gttgactttg tcactgctga caagtttgat gagaatgcca agactggcca agccactgtg
     4021 gcttctggca tacctgctgg ctggatgggc ttggactgtg gtcctgaaag cagcaagaag
     4081 tatgctgagg ctgtcactcg ggctaagcag attgtgtgga atggtcctgt gggggtattt
     4141 gaatgggaag cttttgcccg gggaaccaaa gctctcatgg atgaggtggt gaaagccact
     4201 tctaggggct gcatcaccat cataggtggt ggagacactg ccacttgctg tgccaaatgg
     4261 aacacggagg ataaagtcag ccatgtgagc actgggggtg gtgccagttt ggagctcctg
     4321 gaaggtaaag tccttcctgg ggtggatgct ctcagcaata tttagggatc caccggatct
     4381 agataactga tcataatcag ccataccacg tttaaacctg gaaaaacatg gagcaatcac
     4441 aagtagcaac acagcagcta ccaatgctgc ttgtgcctgg ctagaagcac aagaggagga
     4501 ggaggtgggt tttccagtca cacctcagac aatcaacctc tggattacaa aatttgtgaa
     4561 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4621 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4681 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4741 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4801 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4861 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4921 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4981 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5041 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5101 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca
     5161 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5221 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5281 ctgattgaca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     5341 gctacaagct agtaccagtt gagccagata agatagaaga ggccaataaa ggagagaaca
     5401 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     5461 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggact
     5521 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5581 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5641 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5701 ctagcagggc cgctttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5761 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5821 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5881 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5941 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     6001 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6061 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6121 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc
     6181 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6241 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6301 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6361 gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6421 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6481 gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6601 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6661 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     6721 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6781 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     6841 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     6901 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     6961 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     7021 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     7081 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     7141 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     7201 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     7261 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7321 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7381 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7441 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7501 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7561 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7621 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7681 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7741 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7801 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7861 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7921 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7981 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8041 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8101 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8161 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8221 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8281 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8341 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8401 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8461 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8521 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8581 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8641 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8701 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8761 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8821 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8881 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8941 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9001 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9061 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9121 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9181 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9241 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9301 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9361 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9421 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9481 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9541 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9601 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9661 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9721 acgtc
//
