LOCUS       hSyn_PKM1       10061 bp DNA     circular SYN 06-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 6, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
FEATURES             Location/Qualifiers
     source          1..10061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3106..4701
                     /codon_start=1
                     /label=hPKM1
                     /translation="MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNT
                     GIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPIL
                     YRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKN
                     ICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVS
                     EKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFD
                     EILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRP
                     TRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELV
                     RASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQ
                     TARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRP
                     GSGFTNTMRVVPVP"
     misc_feature    4716..4726
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     misc_feature    4867..5455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4920..4940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5338..5349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5862..6042
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6068..6085)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6074..6298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6344..6772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6431..6450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6641..6662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6781..6801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6786..7116
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6967..7102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7029..7048
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7164..7211
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7230..7604
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7734..7855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7771..7790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7825..7844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7904..7920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7904..7920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7917..7939)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7928..7944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7952..7958,7959..7976,7977..7982))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7997..8018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8135..8152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8306..8894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8386..8405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9065..9856,9857..9925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9688..9707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9926..10030)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagagatc cgctagcgct
     3061 accggactca gatctcgagc tcaagcttcg aattctgcag tcgacatgtc gaagccccat
     3121 agtgaagccg ggactgcctt cattcagacc cagcagctgc acgcagccat ggctgacaca
     3181 ttcctggagc acatgtgccg cctggacatt gattcaccac ccatcacagc ccggaacact
     3241 ggcatcatct gtaccattgg cccagcttcc cgatcagtgg agacgttgaa ggagatgatt
     3301 aagtctggaa tgaatgtggc tcgtctgaac ttctctcatg gaactcatga gtaccatgcg
     3361 gagaccatca agaatgtgcg cacagccacg gaaagctttg cttctgaccc catcctctac
     3421 cggcccgttg ctgtggctct agacactaaa ggacctgaga tccgaactgg gctcatcaag
     3481 ggcagcggca ctgcagaggt ggagctgaag aagggagcca ctctcaaaat cacgctggat
     3541 aacgcctaca tggaaaagtg tgacgagaac atcctgtggc tggactacaa gaacatctgc
     3601 aaggtggtgg aagtgggcag caagatctac gtggatgatg ggcttatttc tctccaggtg
     3661 aagcagaaag gtgccgactt cctggtgacg gaggtggaaa atggtggctc cttgggcagc
     3721 aagaagggtg tgaaccttcc tggggctgct gtggacttgc ctgctgtgtc ggagaaggac
     3781 atccaggatc tgaagtttgg ggtcgagcag gatgttgata tggtgtttgc gtcattcatc
     3841 cgcaaggcat ctgatgtcca tgaagttagg aaggtcctgg gagagaaggg aaagaacatc
     3901 aagattatca gcaaaatcga gaatcatgag ggggttcgga ggtttgatga aatcctggag
     3961 gccagtgatg ggatcatggt ggctcgtggt gatctaggca ttgagattcc tgcagagaag
     4021 gtcttccttg ctcagaagat gatgattgga cggtgcaacc gagctgggaa gcctgtcatc
     4081 tgtgctactc agatgctgga gagcatgatc aagaagcccc gccccactcg ggctgaaggc
     4141 agtgatgtgg ccaatgcagt cctggatgga gccgactgca tcatgctgtc tggagaaaca
     4201 gccaaagggg actatcctct ggaggctgtg cgcatgcagc acctgatagc tcgtgaggct
     4261 gaggcagcca tgttccaccg caagctgttt gaagaacttg tgcgagcctc aagtcactcc
     4321 acagacctca tggaagccat ggccatgggc agcgtggagg cttcttataa gtgtttagca
     4381 gcagctttga tagttctgac ggagtctggc aggtctgctc accaggtggc cagataccgc
     4441 ccacgtgccc ccatcattgc tgtgacccgg aatccccaga cagctcgtca ggcccacctg
     4501 taccgtggca tcttccctgt gctgtgcaag gacccagtcc aggaggcctg ggctgaggac
     4561 gtggacctcc gggtgaactt tgccatgaat gttggcaagg cccgaggctt cttcaagaag
     4621 ggagatgtgg tcattgtgct gaccggatgg cgccctggct ccggcttcac caacaccatg
     4681 cgtgttgttc ctgtgccgtg aggatccacc ggatctagat aactgatcat aatcagccat
     4741 accacgttta aacctggaaa aacatggagc aatcacaagt agcaacacag cagctaccaa
     4801 tgctgcttgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4861 tcagacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4921 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4981 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5041 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5101 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5161 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5221 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     5281 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5341 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5401 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     5461 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     5521 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaaagaaga caagatatcc
     5581 ttgatctgtg gatctaccac acacaaggct acttccctga ttgacagaac tacacaccag
     5641 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     5701 cagataagat agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     5761 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     5821 catttcatca cgtggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     5881 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5941 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6001 gatccctcag acccttttag tcagtgtgga aaatctctag cagggccgct ttaaacccgc
     6061 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6121 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6181 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6241 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6301 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     6361 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6421 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6481 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc
     6541 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6601 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6661 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt
     6721 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     6781 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg
     6841 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6901 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6961 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7021 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     7081 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     7141 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     7201 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     7261 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     7321 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     7381 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     7441 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     7501 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     7561 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     7621 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7681 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     7741 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7801 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7861 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7921 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7981 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8041 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8101 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8161 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8221 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     8521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8821 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8881 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8941 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9001 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9061 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9121 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9181 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9241 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9301 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9361 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9421 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9481 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9541 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9601 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9661 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9721 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9781 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9841 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9901 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9961 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10021 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
