LOCUS       pAAV-CAG-fDIO-hSERT-3xHA-3xmiR122-WPRE-final        7475 bp    DNA     circular UNA 07-MAY-2024
DEFINITION  FLP dependent SERT for Addgene submission.
ACCESSION   urn.local...m-howd2uy
VERSION     urn.local...m-howd2uy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /created_by="tjkro"
                     /label="AAV2 ITR"
     promoter        210..513
                     /created_by="tjkro"
                     /label="CMV_Enhancer"
     promoter        515..834
                     /created_by="tjkro"
                     /label="Chicken_ACTB_Promoter"
     promoter        835..885
                     /created_by="tjkro"
                     /label="Chicken_ACTB_Exon1"
     promoter        886..993
                     /created_by="tjkro"
                     /label="Chicken_ACTB_Intron_Pt1"
     promoter        994..1149
                     /created_by="tjkro"
                     /modified_by="tjkro"
                     /label="Chicken_ACTB_Intron_Pt2"
     promoter        1157..1250
                     /created_by="tjkro"
                     /modified_by="tjkro"
                     /label="Rab_Bglobin_Exon3_SpliceAcceptor"
     source          <1268..>1395
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     protein_bind    1273..1306
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
                     /modified_by="tjkro"
                     /label="FRT Recombination Site"
     protein_bind    1357..1390
                     /created_by="tjkro"
                     /modified_by="tjkro"
                     /label="F5 Recombination Site"
     CDS             complement(1396..1398)
                     /created_by="tjkro"
                     /modified_by="tjkro"
                     /label="StopCodon"
     CDS             complement(1399..1425)
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /modified_by="tjkro"
                     /label="HA Tag"
     CDS             complement(1426..1452)
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /modified_by="tjkro"
                     /label="HA Tag"
     CDS             complement(1453..1479)
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /modified_by="tjkro"
                     /label="HA Tag"
     CDS             complement(1480..3369)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /modified_by="tjkro"
                     /Color="#ffff00"
                     /modified_by="tjkro"
                     /label="hSERT CDS - Removed Stop Codon"
     misc_feature    complement(3370..3375)
                     /created_by="tjkro"
                     /label="Kozack"
     source          <3376..>3498
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     unsure          <3376..>3498
                     /created_by="tjkro"
                     /label="From pAAV-Ef1a-fDIO-mCherry"
     protein_bind    complement(3381..3414)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
                     /modified_by="tjkro"
                     /label="FRT Recombination Site"
     protein_bind    complement(3465..3498)
                     /created_by="tjkro"
                     /modified_by="tjkro"
                     /label="F5 Recombination Site "
     misc_feature    3498^3499
                     /Original_Bases="GAATTCGATATCAAGCTTATCGATGAATTCA"
                     /label="GAATTCGATATCAAGCTTATCGATGAATTCA"
                     /note="Geneious type: Editing History Deletion"
     unsure          <3499..7475
                     /created_by="tjkro"
                     /label="From pAAV-CAG-NLS-GFP-miR122-3x-WPRE"
     misc_feature    3504..3525
                     /label="mmu-miR-122-5p Binding site"
     misc_feature    3526..3531
                     /label="spacer 1"
     misc_feature    3532..3553
                     /label="mmu-miR-122-5p Binding site"
     misc_feature    3554..3559
                     /label="spacer 2"
     misc_feature    3560..3581
                     /label="mmu-miR-122-5p Binding site"
     regulatory      3592..4200
                     /modified_by="tjkro"
                     /label="WPRE"
     polyA_signal    4220..4698
                     /modified_by="tjkro"
                     /label="hGH polyA"
     repeat_region   4738..4867
                     /created_by="tjkro"
                     /label="AAV2 ITR"
     rep_origin      4970..5276
                     /direction="RIGHT"
                     /label="f1 Origin"
     promoter        5690..5794
                     /created_by="tjkro"
                     /label="AmpR Promoter"
     misc_feature    5795..6652
                     /label="AmpR"
     rep_origin      6803..7470
                     /direction="RIGHT"
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      841 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      901 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      961 tggctgcgtg aaagccttga ggggctccgg gagcgccggc aggaaggaaa tgggcgggga
     1021 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1081 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1141 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1201 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgcggcccaa
     1261 cggtaccccg gagaagttcc tattctctag aaagtatagg aacttcgcag aatggtagct
     1321 ggattgtagc tgctattagc aatatgaaac ctcttagaag ttcctattct tcaaaaggta
     1381 taggaacttc ggcgcttaag cgtaatctgg aacatcgtat gggtaagcgt aatctggaac
     1441 atcgtatggg taagcgtaat ctggaacatc gtatgggtac acagcattca agcggatgtc
     1501 cccacaagga atttctgttg gtgtttctgg ggtaatactt ttaataatac gctctttaaa
     1561 tgtccctgga gtgatgatca accgataagc tatatatgtg gggatgcaaa tgaaagatga
     1621 ggttcctatg cagtaaccca agatgatact ccagtaagga taattatatt ggaaaagtcg
     1681 tagttgtggc gggctcatca gaaaactgca aatgatgaac aggagaaaca gagggctgat
     1741 ggccacccag cagatcctcc agaaccaccc cgggctgaag ccgagcattt ccttcacgtc
     1801 cctgcagaac tgagtgatgc catagaacca agacacagcg actgcttcga tcagcgcgac
     1861 agtgagcact gcgggccccg tggcatactc ctccagcagc ttcaccacgt aggcccctcc
     1921 aaaagtcagg gtgaccaggg atccaaagaa gcaggtgatg accacggcga gcacgaaccg
     1981 ctcccggcgc ttggcccaga cgtgtgggaa ctcatccagc acagccgtga tcaccccctc
     2041 caagcctgca aacgtgctgt ccaagcccag cgtgattaac atcagaaaga agatgatggc
     2101 aaagaaagtg gacgctggca tgttggctat cgcttctgca tacgtgatga agaggaggct
     2161 gggacctgcg tctttggcca cctcagacac atcttcattc ctcatctcag ccatgtaacc
     2221 gagcactgtg aagatgacaa atcccgaaac gaagctcgtc atgcagttca ccacgctggt
     2281 caccagggca tcttggtagc agttgttgtt gaacttgttg tagctagcaa aagccagcag
     2341 gaccccaaag cccggaccaa gagagaagaa gatctgagcg gctgcatcta tccacacccc
     2401 tgtctccagg agtttctgcc aattgggttt caagtagaag agaacacccc tccaggctcc
     2461 agggagggtg gcacccctca ccagcaggac agaaaggatg atataaggga aggtggctgt
     2521 cacccacacc accttgccag aggtcttgac gcctttccag atgctgaagt agataacagt
     2581 gaagatcagc atgatgcaga gggccagctg ccagctgatg ccccccaggt cctggagccc
     2641 cttagaccgg tggatctgca ggacgtggcg cgtgtaaaat tcttcagcag gggacgtgga
     2701 atggagggtc caggtgatgt tgtcctcgga gaagtaattg gtgcagttgc cagtgttcca
     2761 ggagttcttg cagctggtcc agggcagctg gtccgtgaag gaggagatga ggtagtatag
     2821 cgcccaggcc atgatggtgt tgtagtagga agcaatgtaa aaggcaatga tgcagatggc
     2881 ataaccaatc cctttgaaaa tcgggcagat tttcctccat attgaaatgc atccatttcg
     2941 gtggtactgt cccagtgcga gctccatgta aaagagcggg attcccccaa aaatggccat
     3001 gatggtgtag gggaggagga atgccccccc tccattctgg taacatatgt aggggaagcg
     3061 ccagacattg cccaggtcca cagcatagcc aatcactgag agaaggaaat ccaccttctt
     3121 gccccaggtc tcccgttccc cttgatgaag ctcagccact agggtggtgg tggtcgctgg
     3181 gatagagtgc cgtgtgtcat ctcccgcacc aggacttgga actgctgagt acccattgga
     3241 tatttgcccg gactccactt tgtcccctgg ggtgggaaca accttctgta gaactccgtt
     3301 ttcctgacaa tcttctccat cttcacacgc tgatagctgc ttctgagaat tcaagggcgt
     3361 cgtctccatg gtggcctagc gaagttccta tactttctag agaataggaa cttcttgcct
     3421 taacccagaa attatcactg ttattcttta gaatggtgca aagagaagtt cctatacctt
     3481 ttgaagaata ggaacttccc ggtcaaacac cattgtcaca ctccatacta tcaaacacca
     3541 ttgtcacact ccagatcaac aaacaccatt gtcacactcc acgccggcga agcttatcga
     3601 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3661 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3721 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3781 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3841 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3901 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3961 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4021 cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4081 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4141 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     4201 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     4261 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     4321 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     4381 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     4441 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     4501 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     4561 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     4621 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     4681 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     4741 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4801 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4861 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4921 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4981 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     5041 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5101 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5161 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5221 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5281 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     5341 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     5401 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5461 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5521 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5581 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5641 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5701 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5761 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5821 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5881 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5941 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6001 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6061 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6121 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6181 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6241 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6301 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6361 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6421 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6481 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6541 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6601 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6661 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6721 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6781 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6841 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6901 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6961 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7021 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7081 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7141 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7201 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7261 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7321 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7381 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7441 gttcctggcc ttttgctggc cttttgctca catgt
//
