LOCUS       1203M_maxi_2_04              8174 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             7144..8004
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(6390..6769)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    complement(1062..1286)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
     promoter        complement(6186..6389)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        7039..7143
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     CDS             complement(1315..1333)
                     /note="pLannotate"
                     /label="8xHis"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="79.16666666666666"
                     /fragment="True"
                     /other="CDS"
     misc_feature    6924..6968
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="32.37410071942446"
                     /fragment="True"
                     /other="misc feature"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="98.936"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(1037..1058)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="6.197183098591549"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctcttcccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcatcctag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtatc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
     1081 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     1141 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     1201 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     1261 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg
     1321 atggtgatga tgaccggttt atgcccacgg tgtccacggc gtggtcagct tcttctgccg
     1381 atcttctttc ttgaattccc cgtctttgag ttcttcgtaa ccgctattcc cattaggatc
     1441 gtccttcttt cgcagcatgg ggtagcaaac ccggggtgct tggtcgccct caggaaacca
     1501 aagaagtttc ttaagatttt ctataaaatc gagctcgtcc cggaaccatt ccttcagttt
     1561 agcaaatcct tttcccaacc agttgggaat ttcggaattg ccattccgcc attgcttcca
     1621 atcttttctc aggcgcactg attcgacgtc aatttcaact gaaccaaatc ccatagactt
     1681 tgccattcca agtttatgcg ccagaccctt ttcgagttgc aggctgtgga tgagcaaacc
     1741 cagctcccat tctttcaggt tctcaaaggc aatttcgaag ctaaaggaat tcccgccggc
     1801 gagagcttct acagtgcggt tattagggct ttgttcaata gctttgcctg ttgtgggatg
     1861 gttcccgtcc ttaatggttt tccaggcgtg gtggtggacg taaaactttc tgccaggcac
     1921 tttaaactta ttgtcggatc ccggtatact ccaagtcggg cgaggccgtt ccaacaatga
     1981 gagcatcacc ggcccgccgt gaaacggatc gccgggattc ttgcctggga tcagccactc
     2041 cggatcgttt tcgagtgacg cgaatccaaa cctgactctg cccttgtaag atccggttcc
     2101 aaagagccgg caagcagggc agagtccttt ggggtggcgc aagaggagtc ttttctcggg
     2161 atatgcattc aaggcactga gatcaccatc ttccacccaa tctccatggc acggccgcag
     2221 atcggcgctc atacgtttgc cgatcatcct atcgtctaca gttcgggaaa tccgcactgg
     2281 gacgatgtcc tctacgtact tttcattatg tttgaaatac acaagatctc cggacttcag
     2341 cttttccacg ttttcccggg caactctaga ctgaaatacg ctctccggca ctgcttgagg
     2401 attgttgtta tagaccctga gcagttcttt gtatttgatt cttgctttct cggggatctc
     2461 gtattcctcg ttctccttca gatcgaagaa gaaggtttcg caatatttag ccatggtata
     2521 gtaattgccc ttatcatcct cacaacaaaa cacaggttca ttcttccttt tcctattctt
     2581 aaaatcgttc gtcctaattg ttttccagtc gttgggaact gatgggagtg ttccttgaaa
     2641 gttattgatc acatcccctt tcttgtcact aaactctact ttgctggggc cgacgacatg
     2701 aaggtagccc tctttacact cccatccgtc tgggtcgttc tcgggtatct cttgaaaggc
     2761 cttttggtat ctactatctc ttacccagca atccagtttc tcaccattct gtatgccttc
     2821 gagtttctcc tggtcaaaat tgtcaggttt atgccacaag tggaaagaag tgcagccatt
     2881 attggtgacc acgttaaaat aataagagcc attcttttgc tctataaatg tggcgatgcc
     2941 ttctttgcgt cttttccatt tattgtcttg tttcttctgg gggtgtttag caggatcaac
     3001 gaggctcagt cgtttaataa aacgcttgac ccacatccga acaggtttct caccagcaat
     3061 ttcttgctca tcaagaatgt caaaatgttt ctgcgttttg tcatagaaag gcacccttgc
     3121 agtttcggaa aacttttgta tatgtttccc atcggcggtc actcgacctg ggagaaagtc
     3181 ttgcagcaca ttctggtgat cagcatccat ccgccatgac aaccgtttag tctcatcaaa
     3241 tatcctgaag caagaatttg tcacagtttc gtagacacta ctcaccattc ctctcaattc
     3301 gctcccggga atcatgattt gtttatgaag tctgaagaaa gcatatgatt tatgatattc
     3361 gtccttttcc ttccgagctt ctttatccgc aggtctgaag aaatcgtcat tactagtatc
     3421 tgggacgatg aggggtgtct tggtaatgag cttacaccga attttacctg tcagtcgccc
     3481 ctcgtggaac ttttgatgac cacagggttt ctcctcgcgt tccaccttct tatggggttc
     3541 gacgaaatag tgtgggtaat atactttctc tgcctcgatg cgaacctcgg catcagtttt
     3601 gggcttttct ggaacggcta catctgtctc atcaaaggca ggattcatga ggcgagaaat
     3661 cctcttatca tctcccttta tacccagact cttgacctgt ccgtagccga tggcgctctt
     3721 tccaccgagg ggataaaggc cattattcac atctgcaaga gccttaccga gtgccttaca
     3781 gtactcttcg tcctcgagaa cgtccctgcg gatccagaag gagcctttca acatgagcgg
     3841 tcttgcaggg ctaccgggca gaggggaatc atcgaatttc ttcttatcag ctgcaccgcc
     3901 agtgaatcta tcgatggcca catgatcgaa ggtcacaggt tccgggtcac tttcgaatac
     3961 gagatcttca aaccttattt tgccggcttc atactcactt ccaaagattt ggcacaggag
     4021 acattcacag tcactgtgag tcaactcagt caaagggacg ccatcttcca tatgaatgcg
     4081 tgccacggca cttctgatca cccctctaaa agattcggcc ttgtatgcac atacgggttt
     4141 cgcctcttcc ccttcagctt tatacttaac gaaggtaacc accgctgtac cacgtttgtc
     4201 cacggctgcg cggattgggt cgccattaat aaaagggctt gccatctcaa tggaaacgtt
     4261 aatctcgtgc cattttggat ccctataatt accgggctct ggcagacctg agttcagtcg
     4321 tttcttcagt tcctcgagcc ccttctcgtc acgccatccc caattcttaa gatagtcatt
     4381 cctttgattc tcgtcgctca gatccagcgt ttcatattta gcattttcca tccggaatcg
     4441 gcccttcccg gttgaggcag cgccactcat gaatgcctgc ccctcagccc accatttcaa
     4501 caccgttttc agtgcatcgg gcagcccatc ttcagagccc cggtaccgga gttgaaatgg
     4561 aaagacaatc ccctcggggg ctacttccat attaaacagg gcaccttccg caaccgtgcc
     4621 tgtgaaggga ttaatgcggg tacgatgcct tatttctggc ggggcattgt actcactgcg
     4681 cgcatccatc accgtgatcc ctctcataat cctacacgtc ttacacatgc atggccgtcc
     4741 acccaactca gcattgcatg ggctatcaaa ataggtttga agatctcgac ggagtatacc
     4801 tcgcacggcg cttcttggca gtctatactt gttatcggga gtgaggagta cttgcagtgc
     4861 ggtttgtttt gcatcttcgt ctatcgctcc gaagaagaag ggcgttttag cgaccagttc
     4921 gccgcatacc acagtttctt tcaacacaga tccgattgac acagaccggc tcttttcaag
     4981 ccgatcgggc tttccatgga attcagcatc gcccagtatt ctccgagtaa ttttcaggcc
     5041 gccactcatg tctttactct tgaggtacag cgcttcaccc agcttctcgc agaattctct
     5101 ccattttccg gcattcttga gttccttggt gtctgctgag gtcgtaagga tttgcctaat
     5161 agtgacagag ttttcgtctt tcttcttctt gccgatatcc cagaggtaat ggtcatcctt
     5221 cccgtcatga tcctttggca agccagcgac aagtttacta gagcggcgga gtgaacgaat
     5281 agcatcagca agcaggcggg tatattcggt tttcttatta tcgtccagaa tgctaataat
     5341 ttgctctgcg gtcttttcag cgagattagc attcggttct gcttggacat tttcggtttg
     5401 tttcccacta tcggctgctg gtgtatattc atcgaacctg attacgcaca gagctccaca
     5461 cagccgatcc gtaaacttga gagaatcaca gagcagtttc ctggcttcag cgctgacttt
     5521 attatctatg gtaatctccc cttcgaagcg gggaaaccta gtatggtcga cttcgtaagc
     5581 cttaaagaag tcatgggcct taccggattt gaaatcgacc ctgttaagta cccgttggct
     5641 gcctatagct tttggcccgt cgaaatcggg cttccctgga agggaaagat tgccaaaatg
     5701 aatacgaaag cgccaatcct tcttctcagc cttctttcca tcgttaccgg atctcccgag
     5761 aagttcgcaa aacgggcagt aagtttcagc gttatctgga caggggttct tatcggtcca
     5821 acgaagggtg gaccgctgcc tcaattgcag caacctgtct ttatcttccg tatcaaattt
     5881 cccaggacaa catgtctttt cgctaatttt cccatcactc aatgtcagca ggttttccgc
     5941 gcttctaatt actgcggacc gcagcaatgt cccagttatg taaggccgcc cttttgtgtt
     6001 atccttctta tttctatgcc atcttgcaaa agcttggccg cgaacaaatt ccttgttgtt
     6061 cttgtttcgc ctagtgcttt cctgccattt ggtcatccga aacggctcga ggaattcaat
     6121 tgaaatcttc atagtagtcg tcatgcggcc gcggatctct agcggatctg acggttcact
     6181 aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
     6241 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
     6301 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
     6361 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
     6421 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     6481 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     6541 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     6601 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     6661 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     6721 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat
     6781 ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa
     6841 ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta ctcagcgacc
     6901 tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga gcagattgta
     6961 ctgagagtcg accctagggg atcgggagat cgatctcccg atccgtcgac gtcaggtggc
     7021 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7081 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7141 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7201 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7261 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7321 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7381 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7441 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7501 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7561 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7621 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7681 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7741 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7801 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7861 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7921 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7981 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8041 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8101 atgaccaaaa tcccttaacg tgagttttcg ttccactgag tgtcagaccc cgtagaaaag
     8161 atcaaaggat cttc
//
