LOCUS       pCas_dCas12f1_LVA       5336 bp ds-DNA     circular     07-MAY-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     gene            complement(554..1213)
                     /label="CAT/CamR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: CAT/CamR"
     origin          1575..2414
                     /label="p15A_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: p15A_origin"
     primer          2384..2408
                     /label="BG20582"
                     /note="sequence: tcaagaagatcatcttattaatcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2573..2607
                     /label="PJ23108"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: PJ23108"
     misc_feature    2606..2624
                     /label="FlankL"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: FlankL"
     CDS             2625..3890
                     /label="AsCas12f"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: AsCas12f transl_table: 1"
     CDS             2625..3902
                     /label="CDS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 transl_table: 1 translation: MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIMGIALGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESIKLVA*"
     CDS             2625..3902
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIMGIALGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESIKLVA*"
     primer          3098..3119
                     /label="BG27089"
                     /note="sequence: CGTTAAACGTAAAATCTCCGTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3297..3299
                     /label="D225A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: D225A"
     primer          complement(3870..3899)
                     /label="BG30110"
                     /note="sequence: agctactaaTTTGATGCTTTCCGCGATGAT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3891..3899
                     /label="LVA tag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3900..3920
                     /label="BG30109"
                     /note="sequence: TAAaagcttggctgttttggc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3903..3921
                     /label="FlankR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: FlankR"
     terminator      3991..4148
                     /label="rrnB_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_terminator"
     terminator      4114..4157
                     /label="rrnB_T1_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_T1_terminator"
     terminator      4289..4316
                     /label="rrnB_T2_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_T2_terminator"
     promoter        4357..4385
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: AmpR_promoter"
     primer          complement(4373..4397)
                     /label="BG23146"
                     /note="sequence: tttatcagggttattgtctcatgag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     gene            4554..4838
                     /label="Ampicillin (576 - 860)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: Ampicillin (576 - 860)"
     origin          4897..5196
                     /label="f1_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: f1_origin"
ORIGIN
        1 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
       61 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      241 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtcaggcat ttgagaagca
      361 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
      421 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
      481 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac
      541 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      601 gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca
      661 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
      721 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
      781 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
      841 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
      901 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
      961 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
     1021 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1081 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1141 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1201 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1261 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     1321 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     1381 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg
     1441 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     1501 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     1561 accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg
     1621 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
     1681 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     1741 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     1801 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     1861 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     1921 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     1981 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     2041 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     2101 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     2161 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     2221 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     2281 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     2341 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     2401 ttaatcagat aaaatatttg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     2461 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     2521 tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tcatcgatgc atctgacagc
     2581 tagctcagtc ctaggtataa tgctagcgag ctcAGGAGGA CATCATGATC AAAGTTTACC
     2641 GTTATGAAAT CGTTAAACCG CTGGACCTGG ATTGGAAAGA ATTCGGCACC ATCCTGCGTC
     2701 AGCTGCAGCA GGAAACCCGC TTCGCGCTGA ACAAAGCAAC CCAGCTGGCG TGGGAATGGA
     2761 TGGGTTTCAG CTCTGATTAC AAAGACAACC ATGGCGAATA CCCGAAAAGC AAAGACATCC
     2821 TGGGCTACAC CAACGTTCAC GGTTACGCAT ACCACACCAT CAAAACCAAA GCTTATCGTC
     2881 TGAACAGCGG CAACCTGAGC CAGACCATCA AACGTGCGAC CGATCGCTTC AAAGCGTACC
     2941 AGAAAGAAAT CCTGCGTGGT GACATGTCCA TCCCGTCTTA CAAACGTGAT ATCCCGCTGG
     3001 ATCTGATCAA AGAAAACATC AGCGTTAACC GTATGAACCA CGGTGATTAT ATCGCTTCCC
     3061 TGTCCCTGCT GTCTAACCCG GCGAAACAGG AAATGAACGT TAAACGTAAA ATCTCCGTGA
     3121 TTATCATCGT TCGTGGCGCG GGTAAAACCA TCATGGATCG CATCCTGAGC GGCGAATACC
     3181 AGGTGAGCGC GAGCCAGATC ATCCACGACG ACCGTAAAAA CAAATGGTAC CTGAACATCT
     3241 CTTATGATTT CGAACCGCAG ACCCGTGTTC TGGATCTGAA CAAAATCATG GGCATCGcTC
     3301 TGGGTGTTGC GGTGGCGGTG TACATGGCGT TCCAGCACAC CCCGGCGCGC TACAAACTGG
     3361 AAGGCGGTGA AATCGAAAAC TTCCGTCGTC AGGTGGAGTC TCGTCGCATC TCCATGCTGC
     3421 GCCAGGGTAA ATACGCGGGT GGTGCTCGTG GCGGTCACGG CCGTGATAAA CGTATCAAAC
     3481 CGATCGAACA GCTGCGTGAC AAAATCGCTA ACTTCCGCGA TACCACTAAC CACCGTTACT
     3541 CTCGTTACAT CGTTGATATG GCTATCAAAG AAGGTTGCGG TACCATTCAG ATGGAAGATC
     3601 TGACCAACAT CCGTGATATC GGCTCTCGTT TCCTGCAGAA CTGGACCTAC TACGATCTGC
     3661 AGCAGAAAAT CATCTACAAA GCGGAAGAAG CGGGCATCAA AGTTATCAAA ATTGATCCGC
     3721 AGTACACCAG CCAGCGTTGC AGCGAATGCG GCAACATCGA TTCCGGTAAC CGTATCGGTC
     3781 AGGCGATCTT CAAATGCCGT GCTTGCGGCT ACGAAGCGAA CGCGGACTAC AACGCAGCTC
     3841 GTAACATCGC GATCCCGAAC ATCGATAAAA TCATCGCGGA AAGCATCAAA ttagtagctT
     3901 AAaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
     3961 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     4021 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     4081 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     4141 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     4201 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     4261 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     4321 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4381 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4441 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4501 agaaacgctg gtgaaagtaa aagatgctga agatcagttg gggcaaacta ttaactggcg
     4561 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4621 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4681 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4741 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4801 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4861 atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4921 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4981 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5041 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     5101 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5161 tccacgttct ttaatagtgg actcttgttc caaacttgaa caacactcaa ccctatctcg
     5221 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5281 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattta
//