LOCUS       pCas_dCas12f1_CBE1_LVA  6353 bp ds-DNA     circular     07-MAY-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     origin          228..527
                     /label="f1_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: f1_origin"
     gene            complement(1221..1880)
                     /label="CAT/CamR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: CAT/CamR"
     primer          2163..2184
                     /label="BG26483"
                     /note="sequence: cttctgtttctatcagctgtcc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     origin          2242..3081
                     /label="p15A_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: p15A_origin"
     primer          3051..3075
                     /label="BG20582"
                     /note="sequence: tcaagaagatcatcttattaatcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3129..3147
                     /label="BG20191"
                     /note="sequence: gatgtcggcgatataggcg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3240..3274
                     /label="PJ23108"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: PJ23108"
     misc_feature    3273..3291
                     /label="Flank L"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: Flank L"
     CDS             3292..4557
                     /label="AsCas12f"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: AsCas12f transl_table: 1"
     CDS             3292..5586
                     /label="CDS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 transl_table: 1 translation: MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIMGIALGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESIKSGSETPGTSESATPESMSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHILWATGLKPKKKRKVSGGSTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLLVA*"
     CDS             3292..5586
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIMGIALGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESIKSGSETPGTSESATPESMSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHILWATGLKPKKKRKVSGGSTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLLVA*"
     primer          3765..3786
                     /label="BG27089"
                     /note="sequence: CGTTAAACGTAAAATCTCCGTG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3964..3966
                     /label="D225A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: D225A"
     misc_feature    4548..4551
                     /label="overlap region for BbsI sites"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4558..4605
                     /label="XTEN"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4606..4623
                     /label="BG26102"
                     /note="sequence: atgagctcagagactggc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             4606..5292
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHILWATGLK"
     misc_feature    4606..5292
                     /label="APOBEC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: APOBEC"
     CDS             5326..5574
                     /label="UGI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: UGI transl_table: 1"
     misc_feature    5575..5583
                     /label="LVA tag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5587..5605
                     /label="FlankR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: FlankR"
     primer          complement(5632..5655)
                     /label="BG20724"
                     /note="sequence: gcttctgcgttctgatttaatctg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     terminator      5675..5832
                     /label="rrnB_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_terminator"
     primer          complement(5720..5739)
                     /label="BG26248"
                     /note="sequence: acggcgtttcacttctgagt"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     terminator      5798..5841
                     /label="rrnB_T1_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_T1_terminator"
     terminator      5973..6000
                     /label="rrnB_T2_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_T2_terminator"
     promoter        6041..6069
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: AmpR_promoter"
ORIGIN
        1 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
       61 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      121 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
      181 agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg
      241 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      301 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      361 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      421 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      481 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaacttga acaacactca
      541 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      601 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
      661 caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      721 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      781 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      841 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      901 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
      961 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt caggcatttg
     1021 agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat
     1081 aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt
     1141 tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac
     1201 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     1261 tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc
     1321 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     1381 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     1441 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     1501 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     1561 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     1621 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc
     1681 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     1741 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     1801 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     1861 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     1921 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     1981 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     2041 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg
     2101 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     2161 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
     2221 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact
     2281 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc
     2341 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
     2401 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
     2461 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
     2521 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
     2581 aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc
     2641 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
     2701 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
     2761 ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac
     2821 atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag
     2881 tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc
     2941 cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag
     3001 gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat
     3061 catcttatta atcagataaa atatttgctc atgagcccga agtggcgagc ccgatcttcc
     3121 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     3181 gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatca tcgatgcatc
     3241 tgacagctag ctcagtccta ggtataatgc tagcgagctc AGGAGGACAT Catgatcaaa
     3301 gtttaccgtt atgaaatcgt taaaccgctg gacctggatt ggaaagaatt cggcaccatc
     3361 ctgcgtcagc tgcagcagga aacccgcttc gcgctgaaca aagcaaccca gctggcgtgg
     3421 gaatggatgg gtttcagctc tgattacaaa gacaaccatg gcgaataccc gaaaagcaaa
     3481 gacatcctgg gctacaccaa cgttcacggt tacgcatacc acaccatcaa aaccaaagct
     3541 tatcgtctga acagcggcaa cctgagccag accatcaaac gtgcgaccga tcgcttcaaa
     3601 gcgtaccaga aagaaatcct gcgtggtgac atgtccatcc cgtcttacaa acgtgatatc
     3661 ccgctggatc tgatcaaaga aaacatcagc gttaaccgta tgaaccacgg tgattatatc
     3721 gcttccctgt ccctgctgtc taacccggcg aaacaggaaa tgaacgttaa acgtaaaatc
     3781 tccgtgatta tcatcgttcg tggcgcgggt aaaaccatca tggatcgcat cctgagcggc
     3841 gaataccagg tgagcgcgag ccagatcatc cacgacgacc gtaaaaacaa atggtacctg
     3901 aacatctctt atgatttcga accgcagacc cgtgttctgg atctgaacaa aatcatgggc
     3961 atcgctctgg gtgttgcggt ggcggtgtac atggcgttcc agcacacccc ggcgcgctac
     4021 aaactggaag gcggtgaaat cgaaaacttc cgtcgtcagg tggagtctcg tcgcatctcc
     4081 atgctgcgcc agggtaaata cgcgggtggt gctcgtggcg gtcacggccg tgataaacgt
     4141 atcaaaccga tcgaacagct gcgtgacaaa atcgctaact tccgcgatac cactaaccac
     4201 cgttactctc gttacatcgt tgatatggct atcaaagaag gttgcggtac cattcagatg
     4261 gaagatctga ccaacatccg tgatatcggc tctcgtttcc tgcagaactg gacctactac
     4321 gatctgcagc agaaaatcat ctacaaagcg gaagaagcgg gcatcaaagt tatcaaaatt
     4381 gatccgcagt acaccagcca gcgttgcagc gaatgcggca acatcgattc cggtaaccgt
     4441 atcggtcagg cgatcttcaa atgccgtgct tgcggctacg aagcgaacgc ggactacaac
     4501 gcagctcgta acatcgcgat cccgaacatc gataaaatca tcgcggaaag catcaaaagc
     4561 ggcagcgaga ctcccgggac ctcagagtcc gccacacccg aaagtatgag ctcagagact
     4621 ggcccagtgg ctgtggaccc cacattgaga cggcggatcg agccccatga gtttgaggta
     4681 ttcttcgatc cgagagagct ccgcaaggag acctgcctgc tttacgaaat taattggggg
     4741 ggccggcact ccatttggcg acatacatca cagaacacta acaagcacgt cgaagtcaac
     4801 ttcatcgaga agttcacgac agaaagatat ttctgtccga acacaaggtg cagcattacc
     4861 tggtttctca gctggagccc atgcggcgaa tgtagtaggg ccatcactga attcctgtca
     4921 aggtatcccc acgtcactct gtttatttac atcgcaaggc tgtaccacca cgctgacccc
     4981 cgcaatcgac aaggcctgcg ggatttgatc tcttcaggtg tgactatcca aattatgact
     5041 gagcaggagt caggatactg ctggagaaac tttgtgaatt atagcccgag taatgaagcc
     5101 cactggccta ggtatcccca tctgtgggta cgactgtacg ttcttgaact gtactgcatc
     5161 atactgggcc tgcctccttg tctcaacatt ctgagaagga agcagccaca gctgacattc
     5221 tttaccatcg ctcttcagtc ttgtcattac cagcgactgc ccccacacat tctctgggcc
     5281 accgggttga aacccaagaa gaaacggaaa gtgtctggtg gttctACTAA TCTGTCAGAT
     5341 ATTATTGAAA AGGAGACCGG TAAGCAACTG GTTATCCAGG AATCCATCCT CATGCTCCCA
     5401 GAGGAGGTGG AAGAAGTCAT TGGGAACAAG CCGGAAAGCG ATATACTCGT GCACACCGCC
     5461 TACGACGAGA GCACCGACGA GAATGTCATG CTTCTGACTA GCGACGCCCC TGAATACAAG
     5521 CCTTGGGCTC TGGTCATACA GGATAGCAAC GGTGAGAACA AGATTAAGAT GCTCttagta
     5581 gcttaaaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     5641 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     5701 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     5761 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     5821 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     5881 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     5941 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     6001 tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6061 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6121 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6181 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttggggcaa actattaact
     6241 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6301 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tga
//