LOCUS       pCas_dCas12f1_ABE_LVA   5930 bp ds-DNA     circular     07-MAY-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     origin          512..1351
                     /label="p15A_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: p15A_origin"
     misc_feature    1440..1440
                     /label="point mutation (c->t)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1510..1544
                     /label="PJ23108"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: PJ23108"
     misc_feature    1534..1534
                     /label="observed point mutation"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: observed point mutation"
     misc_feature    1543..1561
                     /label="FlankL"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: FlankL"
     CDS             1562..2827
                     /label="AsCas12f"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: AsCas12f transl_table: 1 translation: MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIMGIALGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESIK"
     CDS             1562..3433
                     /label="Translation 1562-3424"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 label: Translation 1562-3424 transl_table: 1 translation: MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIMGIALGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESIKSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINLVA*"
     CDS             1562..3433
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIMGIALGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESIKSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINLVA*"
     misc_feature    2234..2236
                     /label="D225A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: D225A"
     misc_feature    2828..2923
                     /label="XXTEN"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: XXTEN"
     misc_feature    2924..3421
                     /label="TadA8e (ABE8e)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: TadA8e (ABE8e)"
     misc_feature    2987..2989
                     /label="W22R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: W22R"
     misc_feature    3026..3028
                     /label="H35L"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: H35L"
     misc_feature    3062..3064
                     /label="P47A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: P47A"
     misc_feature    3071..3073
                     /label="R50L"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: R50L"
     misc_feature    3170..3172
                     /label="L83F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: L83F"
     misc_feature    3236..3238
                     /label="A105V"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: A105V"
     misc_feature    3236..3238
                     /label="V105W (in ABE8e V105W)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: V105W (in ABE8e V105W)"
     misc_feature    3242..3244
                     /label="D107N"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: D107N"
     misc_feature    3245..3247
                     /label="A108S"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: A108S"
     misc_feature    3251..3253
                     /label="T110R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: T110R"
     misc_feature    3275..3277
                     /label="D118N"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: D118N"
     misc_feature    3284..3286
                     /label="H121N"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: H121N"
     misc_feature    3287..3289
                     /label="H122Y"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: H122Y"
     misc_feature    3356..3358
                     /label="S145C"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: S145C"
     misc_feature    3365..3367
                     /label="F148Y"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: F148Y"
     misc_feature    3374..3376
                     /label="R151P"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: R151P"
     misc_feature    3383..3385
                     /label="E154V"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: E154V"
     misc_feature    3386..3388
                     /label="I155F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: I155F"
     misc_feature    3389..3391
                     /label="K156N"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: K156N"
     misc_feature    3416..3418
                     /label="T165I"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: T165I"
     misc_feature    3419..3421
                     /label="D166N"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: D166N"
     misc_feature    3422..3430
                     /label="LVA tag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3434..3452
                     /label="FlankR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: FlankR"
     terminator      3522..3679
                     /label="rrnB_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_terminator"
     terminator      3645..3688
                     /label="rrnB_T1_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_T1_terminator"
     terminator      3820..3847
                     /label="rrnB_T2_terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: rrnB_T2_terminator"
     promoter        3888..3916
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: AmpR_promoter"
     gene            4085..4369
                     /label="Ampicillin (576 - 860)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: Ampicillin (576 - 860)"
     origin          4428..4727
                     /label="f1_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: f1_origin"
     gene            complement(5421..150)
                     /label="CAT/CamR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: CAT/CamR"
ORIGIN
        1 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
       61 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
      121 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
      181 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
      241 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
      301 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc
      361 gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag
      421 gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg
      481 tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta
      541 tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca
      601 ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg
      661 ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc
      721 ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt
      781 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc
      841 gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct
      901 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
      961 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     1021 gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1081 ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt
     1141 agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc
     1201 tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc
     1261 gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc
     1321 tcaagaagat catcttatta atcagataaa atatttgctc atgagcccga agtggcgagc
     1381 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     1441 ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatca
     1501 tcgatgcatc tgacagctag ctcagtccta ggtataatgc tagcgagctc AGGAGGACAT
     1561 CATGATCAAA GTTTACCGTT ATGAAATCGT TAAACCGCTG GACCTGGATT GGAAAGAATT
     1621 CGGCACCATC CTGCGTCAGC TGCAGCAGGA AACCCGCTTC GCGCTGAACA AAGCAACCCA
     1681 GCTGGCGTGG GAATGGATGG GTTTCAGCTC TGATTACAAA GACAACCATG GCGAATACCC
     1741 GAAAAGCAAA GACATCCTGG GCTACACCAA CGTTCACGGT TACGCATACC ACACCATCAA
     1801 AACCAAAGCT TATCGTCTGA ACAGCGGCAA CCTGAGCCAG ACCATCAAAC GTGCGACCGA
     1861 TCGCTTCAAA GCGTACCAGA AAGAAATCCT GCGTGGTGAC ATGTCCATCC CGTCTTACAA
     1921 ACGTGATATC CCGCTGGATC TGATCAAAGA AAACATCAGC GTTAACCGTA TGAACCACGG
     1981 TGATTATATC GCTTCCCTGT CCCTGCTGTC TAACCCGGCG AAACAGGAAA TGAACGTTAA
     2041 ACGTAAAATC TCCGTGATTA TCATCGTTCG TGGCGCGGGT AAAACCATCA TGGATCGCAT
     2101 CCTGAGCGGC GAATACCAGG TGAGCGCGAG CCAGATCATC CACGACGACC GTAAAAACAA
     2161 ATGGTACCTG AACATCTCTT ATGATTTCGA ACCGCAGACC CGTGTTCTGG ATCTGAACAA
     2221 AATCATGGGC ATCGcTCTGG GTGTTGCGGT GGCGGTGTAC ATGGCGTTCC AGCACACCCC
     2281 GGCGCGCTAC AAACTGGAAG GCGGTGAAAT CGAAAACTTC CGTCGTCAGG TGGAGTCTCG
     2341 TCGCATCTCC ATGCTGCGCC AGGGTAAATA CGCGGGTGGT GCTCGTGGCG GTCACGGCCG
     2401 TGATAAACGT ATCAAACCGA TCGAACAGCT GCGTGACAAA ATCGCTAACT TCCGCGATAC
     2461 CACTAACCAC CGTTACTCTC GTTACATCGT TGATATGGCT ATCAAAGAAG GTTGCGGTAC
     2521 CATTCAGATG GAAGATCTGA CCAACATCCG TGATATCGGC TCTCGTTTCC TGCAGAACTG
     2581 GACCTACTAC GATCTGCAGC AGAAAATCAT CTACAAAGCG GAAGAAGCGG GCATCAAAGT
     2641 TATCAAAATT GATCCGCAGT ACACCAGCCA GCGTTGCAGC GAATGCGGCA ACATCGATTC
     2701 CGGTAACCGT ATCGGTCAGG CGATCTTCAA ATGCCGTGCT TGCGGCTACG AAGCGAACGC
     2761 GGACTACAAC GCAGCTCGTA ACATCGCGAT CCCGAACATC GATAAAATCA TCGCGGAAAG
     2821 CATCAAAtct ggtggttctt ctggtggttc tagcggcagc gagactcccg ggacctcaga
     2881 gtccgccaca cccgaaagtt ctggtggttc ttctggtggT TCTTCTGAGG TGGAGTTTTC
     2941 CCACGAGTAC TGGATGAGAC ATGCCCTGAC CCTGGCCAAG AGGGCACGGG ATGAGAGGGA
     3001 GGTGCCTGTG GGAGCCGTGC TGGTGCTGAA CAATAGAGTG ATCGGCGAGG GCTGGAACAG
     3061 AGCCATCGGC CTGCACGACC CAACAGCCCA TGCCGAAATT ATGGCCCTGA GACAGGGCGG
     3121 CCTGGTCATG CAGAACTACA GACTGATTGA CGCCACCCTG TACGTGACAT TCGAGCCTTG
     3181 CGTGATGTGC GCCGGCGCCA TGATCCACTC TAGGATCGGC CGCGTGGTGT TTGGCGTGAG
     3241 GAACTCAAAA AGAGGCGCCG CAGGCTCCCT GATGAACGTG CTGAACTACC CCGGCATGAA
     3301 TCACCGCGTC GAAATTACCG AGGGAATCCT GGCAGATGAA TGTGCCGCCC TGCTGTGCGA
     3361 TTTCTATCGG ATGCCTAGAC AGGTGTTCAA TGCTCAGAAG AAGGCCCAGA GCTCCATCAA
     3421 Cttagtagct TAAaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
     3481 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     3541 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     3601 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     3661 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     3721 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     3781 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     3841 gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat
     3901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3961 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4021 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt ggggcaaact
     4081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4381 agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg
     4441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4621 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaacttga acaacactca
     4741 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4861 caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5101 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt caggcatttg
     5221 agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat
     5281 aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt
     5341 tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac
     5401 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     5461 tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc
     5521 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     5581 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     5641 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     5701 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     5761 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     5821 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc
     5881 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
//