LOCUS       Exported                9263 bp ds-DNA     circular SYN 13-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-FRT-TO-FLAG-TONSL (siR) features
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2017 from SnapGene Viewer 3.0.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    820..838
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    841..859
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     CDS             993..1016
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an
                     enterokinase cleavage site"
                     /note="FLAG"
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     polyA_signal    5221..5445
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    5729..5776
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="color: #31849b; direction: RIGHT"
     CDS             5784..6806
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYH
                     WQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSE
                     AMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQS
                     LVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPST
                     RPRAKE"
     polyA_signal    6936..7057
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7106..7122)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    7130..7146
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7154..7184)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                     1: 7154 .. 7160 / #ffffff / -10 2: 7161 .. 7178 / #ffffff
                     3: 7179 .. 7184 / #ffffff / -35"
     protein_bind    7199..7220
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the
                     presence of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7508..8096)
                     /dnas_title="ori"
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8267..9127)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="This feature has 2 segments: 1: 8267 .. 9058 /
                     #ccffcc 2: 9059 .. 9127 / #ccffcc / signal sequence
                     Cleavage site after base 9058"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(9128..9232)
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1041..5174
                     /note="TONSL"
     CDS             4437..4455
                     /note="siRNA #1 target"
                     /translation="ELDLSM"
                     /note="capitals = mutations introduced for siRNA
                     resistance"
     source          1..9263
                     /dnas_title="pcDNA-FRT-TO-FLAG-TONSL (siR) features2"
     CDS             990..5174
                     /note="FLAG-TONSL siR"
                     /translation="MDYKDDDDKAASLNGAPMSLERELRQLSKAKAKAQRAGQRREEA
                     ALCHQLGELLAGHGRYAEALEQHWQELQLRERADDPLGCAVAHRKIGERLAEMEDYPA
                     ALQHQHQYLELAHSLRNHTELQRAWATIGRTHLDIYDHCQSRDALLQAQAAFEKSLAI
                     VDEELEGTLAQGELNEMRTRLYLNLGLTFESLQQTALCNDYFRKSIFLAEQNHLYEDL
                     FRARYNLGTIHWRAGQHSQAMRCLEGARECAHTMRKRFMESECCVVIAQVLQDLGDFL
                     AAKRALKKAYRLGSQKPVQRAAICQNLQHVLAVVRLQQQLEEAEGRDPQGAMVICEQL
                     GDLFSKAGDFPRAAEAYQKQLRFAELLDRPGAERAIIHVSLATTLGDMKDHHGAVRHY
                     EEELRLRSGNVLEEAKTWLNIALSREEAGDAYELLAPCFQKALSCAQQAQRPQLQRQV
                     LQHLHTVQLRLQPQEAPETETRLRELSVAEDEDEEEEAEEAAATAESEALEAGEVELS
                     EGEDDTDGLTPQLEEDEELQGHLGRRKGSKWNRRNDMGETLLHRACIEGQLRRVQDLV
                     RQGHPLNPRDYCGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCEGITPLHDALN
                     CGHFEVAELLLERGASVTLRTRKGLSPLETLQQWVKLYRRDLDLETRQKARAMEMLLQ
                     AAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCPEPPSNSTRLPEASQVHVRVSPGQA
                     APAMARPRRSRHGPASSSSSSEGEDSAGPARPSQKRPRCSATAQRVAAWTPGPASNRE
                     AATASTSRAAYQAAIRGVGSAQSRLGPGPPRGHSKALAPQAALIPEEECLAGDWLELD
                     MPLTRSRRPRPRGTGDNRRPSSTSGSDSEESRPRARAKQVRLTCMQSCSAPVNAGPSS
                     LASEPPGSPSTPRVSEPSGDSSAAGQPLGPAPPPPIRVRVQVQDHLFLIPVPHSSDTH
                     SVAWLAEQAAQRYYQTCGLLPRLTLRKEGALLAPQDLIPDVLQSNDEVLAEVTSWDLP
                     PLTDRYRRACQSLGQGEHQQVLQAVELQGLGLSFSACSLALDQAQLTPLLRALKLHTA
                     LRELRLAGNRLGDKCVAELVAALGTMPSLALLDLSSNHLGPEGLRQLAMGLPGQATLQ
                     SLEELDLSMNPLGDGCGQSLASLLHACPLLSTLRLQACGFGPSFFLSHQTALGSAFQD
                     AEHLKTLSLSYNALGAPALARTLQSLPAGTLLHLELSSVAAGKGDSDLMEPVFRYLAK
                     EGCALAHLTLSANHLGDKAVRDLCRCLSLCPSLISLDLSANPEISCASLEELLSTLQK
                     RPQGLSFLGLSGCAVQGPLGLGLWDKIAAQLRELQLCSRRLCAEDRDALRQLQPSRPG
                     PGECTLDHGSKLFFRRL"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggactacaa agacgatgac gataaagccg
     1021 ccagtttaaa cggcgcgcca atgagcctgg agcgcgagct tcgccagctg agcaaggcga
     1081 aagccaaggc gcagagggcc gggcagcggc gcgaagaggc cgcgctgtgc caccagctgg
     1141 gggagctcct ggccggccat ggccgctacg ccgaggctct ggagcagcac tggcaggagc
     1201 tgcagcttcg ggagcgcgct gacgaccctc tgggctgtgc cgtggcccac cgcaagatcg
     1261 gagagcgcct ggccgagatg gaggactacc cggctgcctt gcagcaccag caccagtacc
     1321 tggagctggc acattccctg cgcaaccaca cggagctgca gagggcctgg gccaccatcg
     1381 gccgcaccca cctggacatc tatgaccact gccagtcgag ggatgctttg ctgcaggcac
     1441 aggctgcctt tgagaagagc ttggctattg tggatgagga gctggagggg acactggccc
     1501 agggagagct gaatgagatg aggacccgcc tctatctcaa cctgggcctc acctttgaga
     1561 gcctgcagca gacagccctg tgcaacgatt acttcaggaa gagcatcttc cttgcggagc
     1621 agaaccacct ttacgaggac ctattccgcg cccgctacaa cctgggcacc atccactggc
     1681 gcgcgggcca gcactcccag gctatgcgct gcttggaggg tgcccgggag tgtgcgcaca
     1741 ccatgaggaa gcggttcatg gagagcgagt gctgcgtggt tattgcacag gtcctccaag
     1801 acctgggaga ctttttggct gccaagcgag ccctgaagaa ggcctacagg ctgggctccc
     1861 agaagcctgt gcagagggca gccatctgtc agaacctcca gcatgtgctg gcagtggtcc
     1921 ggctgcagca acagctggaa gaggctgagg gcagagaccc tcagggtgcc atggtcatct
     1981 gtgagcagct aggggacctc ttctccaagg caggagactt tcccagggca gctgaggctt
     2041 accagaagca gctgcgtttt gctgagctgc tggacagacc gggtgctgag cgggccatca
     2101 tccacgtgtc cctggccacc acactgggag acatgaagga ccaccatggg gccgtgcgcc
     2161 actatgagga ggaactgagg ctgcgcagcg gcaacgtgct ggaggaggcc aagacctggc
     2221 tgaacattgc actgtcccgc gaggaggccg gcgatgccta cgagctgctg gccccgtgct
     2281 tccagaaagc gctcagctgt gcccagcagg cccagcgtcc ccagctgcag aggcaggtct
     2341 tgcagcatct ccataccgtg cagctgaggc tgcagcccca ggaggcccct gagaccgaaa
     2401 ccagactgcg ggagctcagt gtagctgaag atgaagatga ggaggaggag gcggaggagg
     2461 cggcagccac agcggagagc gaagccctgg aggccggcga ggtggagctc tcagagggcg
     2521 aggacgacac cgatggcctg accccgcagc tggaggagga cgaggagctt cagggccacc
     2581 tgggccggcg gaaggggagc aagtggaacc ggcgaaacga catgggggag accctgctgc
     2641 accgagcctg catcgagggc cagctgcgcc gcgtccagga ccttgtgagg cagggccacc
     2701 cccttaaccc tcgggactac tgtggctgga cacctctgca cgaggcctgc aactacgggc
     2761 atctagaaat tgtccgcttc ctgctggacc acggggccgc agtggacgac ccaggtggcc
     2821 agggctgcga aggcatcacc cccctccacg atgccctcaa ctgtggccac ttcgaggtgg
     2881 ctgagctgct gcttgaacgg ggggcgtccg tcaccctccg cactcgaaag ggcctcagcc
     2941 cgctggagac gctgcagcag tgggtgaagc tgtaccgcag ggacctggac ctggagacgc
     3001 ggcagaaggc cagggccatg gagatgctgc tccaggcggc tgcctcgggc caagatcccc
     3061 acagctccca ggccttccac accccaagca gccttctgtt tgaccccgag acctctcctc
     3121 ctttgagccc ctgcccagaa cccccctcta atagcactag actcccagag gcctctcagg
     3181 tccatgtcag ggtctcccca gggcaggcgg caccagccat ggccaggcct cggaggagca
     3241 ggcatgggcc agccagcagc agcagcagct cagaaggcga ggacagcgca ggccccgcac
     3301 ggccgtccca gaagaggcct cggtgctcgg ccacagcaca acgggtggca gcctggacgc
     3361 ctggccccgc cagcaacagg gaagcagcca cagccagcac cagccgggca gcctaccagg
     3421 cagccatccg gggtgtgggc agtgctcaga gccggctggg gcctggccca ccgcggggcc
     3481 acagcaaagc ccttgccccc caggcagcgc tcatcccgga ggaggagtgc ctggctgggg
     3541 actggctgga gctggacatg cccctgaccc gcagccgccg gccccgcccc cggggcactg
     3601 gagacaaccg caggcccagt agtacctctg ggtcggacag tgaggagagc aggccccgtg
     3661 cccgagccaa gcaggtccgc ctgacctgca tgcagagttg cagtgcgcca gttaacgcag
     3721 ggcccagcag cctggcttca gaacctccag ggagccccag cacccccagg gtctcagagc
     3781 ccagtgggga cagctctgcg gcaggccagc ccttgggtcc ggccccgccc cctcccatcc
     3841 gggttcgagt tcaagttcag gatcatctct tcctcatccc tgtcccacac agcagtgaca
     3901 cccactctgt ggcctggctg gccgagcagg cggcccagcg ctactaccag acctgcgggc
     3961 tgctgcccag gctcacccta cggaaagagg gggccctgct ggccccacag gacctcatcc
     4021 ctgatgtgct gcagagcaat gacgaggtgt tggctgaggt gacttcgtgg gacctgcccc
     4081 cgttgactga ccgctaccgc agggcctgcc agagcctggg gcaaggggag caccaacagg
     4141 tgctgcaggc cgtggagctc cagggcttgg gcctctcgtt cagcgcctgc tccctggccc
     4201 tggaccaggc ccagcttaca cccctgctgc gggccctcaa gctgcacaca gcactccggg
     4261 agctgcgcct ggcagggaac cggctggggg acaagtgtgt ggctgagctg gtggctgccc
     4321 tgggcaccat gcccagcctg gccctccttg acctctcctc caatcacctg ggtcccgaag
     4381 gcctgcgcca gcttgccatg gggctcccag gccaagccac cttgcagagt ttggaggaAT
     4441 tAgaTCtaTC Gatgaacccc ctgggggacg gctgtggcca gtccctggcc tccctcctgc
     4501 acgcctgccc cttactcagc accctgcgcc tgcaggcgtg tggcttcggc cccagcttct
     4561 ttctgagcca ccagacagca ctgggtagtg ctttccaaga tgctgagcac ctgaagaccc
     4621 tgtccctgtc ctacaacgcc ctgggagccc ctgccctggc caggaccctg cagagcctgc
     4681 ccgccggcac cctcctgcac ttagagctca gctccgtggc agccggcaag ggtgattcgg
     4741 acctcatgga gcctgtattc cgatacctgg ccaaggaagg ctgtgctcta gcccacctga
     4801 ccctgtctgc aaaccacctg ggggacaagg ctgttagaga cctgtgcaga tgtctctctc
     4861 tgtgcccctc actcatctca ctggatctgt ctgccaaccc tgagatcagc tgtgccagct
     4921 tggaagagct cctgtccacc ctccaaaagc ggccccaagg ccttagcttc cttggcctgt
     4981 caggctgcgc cgtccagggt cccctgggcc tgggcctgtg ggacaagata gccgcgcagc
     5041 tccgggaact gcagctgtgc agcagacgcc tctgcgctga ggacagggac gccctgcgcc
     5101 agctgcagcc cagtcggccg ggccccggcg agtgcacgct ggaccacggc tccaagctct
     5161 tctttcggcg cctctagctc gagtctagag ggcccgttta aacccgctga tcagcctcga
     5221 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5281 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5341 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5401 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5461 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5521 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5581 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5641 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5701 ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc tattctctag
     5761 aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5821 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5881 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5941 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     6001 ttccggaagt gcttgacatt ggggatttca gcgagagcct gacctattgc atctcccgcc
     6061 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     6121 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     6181 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     6241 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     6301 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     6361 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     6421 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     6481 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6541 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6601 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6661 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6721 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6781 gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc accgccgcct
     6841 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6901 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     6961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7081 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7201 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7261 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7321 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7381 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7441 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7501 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7561 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7621 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7681 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7741 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7801 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7861 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7921 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7981 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8041 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8101 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8161 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8221 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8281 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8341 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8401 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8461 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8521 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8581 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8641 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8701 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8761 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8821 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8881 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8941 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9001 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9061 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9181 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9241 cccgaaaagt gccacctgac gtc
//

