LOCUS       Exported                8610 bp ds-DNA     circular SYN 12-NOV-2019
DEFINITION  MMS22L (Myc-DDK-tagged)-Human MMS22-like, DNA repair protein 
            (MMS22L).
ACCESSION   NM_198468
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 12, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    201..924
                     /label=CMV promoter
     misc_feature    839..856
                     /label=Restriction Enzyme
     misc_feature    953..971
                     /label=T7 promoter
     misc_feature    1020..4749
                     /label=MMS22L
     misc_feature    1020..1028
                     /label=Restriction Enzyme
     misc_feature    4749..4755
                     /label=Restriction Enzyme
     misc_feature    4767..4858
                     /label=Myc-DDK
     misc_feature    4888..5473
                     /label=PolyA signal
     misc_feature    4945..4964
                     /label=Restriction Enzyme
     misc_feature    5749..6421
                     /label=ColE1
     misc_feature    6851..7645
                     /label="Kan/Neo"
     misc_feature    7680..7982
                     /label=SV40 ori
     misc_feature    8068..8545
                     /label=f1 ori
     CDS             1029..4757
                     /note="MMS22L human"
                     /translation="MENCSAASTFLTDSLELELGTEWCKPPYFSCAVDNRGGGKHFSG
                     ESYLCSGALKRLILNLDPLPTNFEEDTLEIFGIQWVTETALVNSSRELFHLFRQQLYN
                     LETLLQSSCDFGKVSTLHCKADNIRQQCVLFLHYVKVFIFRYLKVQNAESHVPVHPYE
                     ALEAQLPSVLIDELHGLLLYIGHLSELPSVNIGAFVNQNQIKLFPPSWHLLHLHLDIH
                     WLVLEILYMLGEKLKQVVYGHQFMNLASDNLTNISLFEEHCETLLCDLISLSLNRYDK
                     VRSSESLMSDQCPCLCIKELWVLLIHLLDHRSKWFVSESFWNWLNKLLKTLLEKSSDR
                     RRSSMPVIQSRDPLGFSWWIITHVASFYKFDRHGVPDEMRKVESNWNFVEELLKKSIS
                     VQGVILEEQLRMYLHCCLTLCDFWEPNIAIVTILWEYYSKNLNSSFSISWLPFKGLAN
                     TMKSPLSMLEMVKTCCCDKQDQELYKSSSSYTIFLCILAKVVKKAMKSNGPHPWKQVK
                     GRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEVEDVASHVLDLLNFLKPAFVTSQ
                     RALIWKGHMAFLLMYAQKNLDIGVLAEKFSCAFREKAKEFLVSKNEEMVQRQTIWTLL
                     SIYIDGVQEVFETSYCLYPSHEKLLNDGFSMLLRACRESELRTVLSFLQAVLARIRSM
                     HQQLCQELQRDNVDLFVQSSLSAKERHLAAVASALWRHFFSFLKSQRMSQVVPFSQLA
                     DAAADFTLLAMDMPSTAPSDFQPQPVISIIQLFGWDDIICPQVVARYLSHVLQNSTLC
                     EALSHSGYVSFQALTVRSWIRCVLQMYIKNLSGPDDLLIDKNLEEAVEKEYMKQLVKL
                     TRLLFNLSEVKSIFSKAQVEYLSISEDPKKALVRFFEAVGVTYGNVQTLSDKSAMVTK
                     SLEYLGEVLKYIKPYLGKKVFSAGLQLTYGMMGILVKSWAQIFATSKAQKLLFRIIDC
                     LLLPHAVLQQEKELPAPMLSAIQKSLPLYLQGMCIVCCQSQNPNAYLNQLLGNVIEQY
                     IGRFLPASPYVSDLGQHPVLLALRNTATIPPISSLKKCIVQVIRKSYLEYKGSSPPPR
                     LASILAFILQLFKETNTDIYEVELLLPGILKCLVLVSEPQVKRLATENLQYMVKACQV
                     GSEEEPSSQLTSVFRQFIQDYGMRYYYQVYSILETVATLDQQVVIHLISTLTQSLKDS
                     EQKWGLGRNIAQREAYSKLLSHLGQMGQDEMQRLENDNT"
     CDS             4776..4805
                     /note="myc tag"
                     /translation="EQKLISEEDL"
     CDS             4824..4847
                     /note="FLAG tag"
                     /translation="DYKDDDDK"
     CDS             1029..4850
                     /note="MMS22L-mycFLAG"
                     /translation="MENCSAASTFLTDSLELELGTEWCKPPYFSCAVDNRGGGKHFSG
                     ESYLCSGALKRLILNLDPLPTNFEEDTLEIFGIQWVTETALVNSSRELFHLFRQQLYN
                     LETLLQSSCDFGKVSTLHCKADNIRQQCVLFLHYVKVFIFRYLKVQNAESHVPVHPYE
                     ALEAQLPSVLIDELHGLLLYIGHLSELPSVNIGAFVNQNQIKLFPPSWHLLHLHLDIH
                     WLVLEILYMLGEKLKQVVYGHQFMNLASDNLTNISLFEEHCETLLCDLISLSLNRYDK
                     VRSSESLMSDQCPCLCIKELWVLLIHLLDHRSKWFVSESFWNWLNKLLKTLLEKSSDR
                     RRSSMPVIQSRDPLGFSWWIITHVASFYKFDRHGVPDEMRKVESNWNFVEELLKKSIS
                     VQGVILEEQLRMYLHCCLTLCDFWEPNIAIVTILWEYYSKNLNSSFSISWLPFKGLAN
                     TMKSPLSMLEMVKTCCCDKQDQELYKSSSSYTIFLCILAKVVKKAMKSNGPHPWKQVK
                     GRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEVEDVASHVLDLLNFLKPAFVTSQ
                     RALIWKGHMAFLLMYAQKNLDIGVLAEKFSCAFREKAKEFLVSKNEEMVQRQTIWTLL
                     SIYIDGVQEVFETSYCLYPSHEKLLNDGFSMLLRACRESELRTVLSFLQAVLARIRSM
                     HQQLCQELQRDNVDLFVQSSLSAKERHLAAVASALWRHFFSFLKSQRMSQVVPFSQLA
                     DAAADFTLLAMDMPSTAPSDFQPQPVISIIQLFGWDDIICPQVVARYLSHVLQNSTLC
                     EALSHSGYVSFQALTVRSWIRCVLQMYIKNLSGPDDLLIDKNLEEAVEKEYMKQLVKL
                     TRLLFNLSEVKSIFSKAQVEYLSISEDPKKALVRFFEAVGVTYGNVQTLSDKSAMVTK
                     SLEYLGEVLKYIKPYLGKKVFSAGLQLTYGMMGILVKSWAQIFATSKAQKLLFRIIDC
                     LLLPHAVLQQEKELPAPMLSAIQKSLPLYLQGMCIVCCQSQNPNAYLNQLLGNVIEQY
                     IGRFLPASPYVSDLGQHPVLLALRNTATIPPISSLKKCIVQVIRKSYLEYKGSSPPPR
                     LASILAFILQLFKETNTDIYEVELLLPGILKCLVLVSEPQVKRLATENLQYMVKACQV
                     GSEEEPSSQLTSVFRQFIQDYGMRYYYQVYSILETVATLDQQVVIHLISTLTQSLKDS
                     EQKWGLGRNIAQREAYSKLLSHLGQMGQDEMQRLENDNTTRTRPLEQKLISEEDLAAN
                     DILDYKDDDDKV"
     CDS             2415..2432
                     /note="siRNA resistance (to siRNA #17)"
                     /translation="KTCCCD"
     source          1..8610
                     /dnas_title="pMMS22L-siR (myc-FLAG)"
ORIGIN      
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagaactgt tctgctgcat cgacgttcct gactgacagc ttagagctgg
     1081 agctggggac ggaatggtgc aaacctcctt acttttcttg tgctgttgac aacagaggag
     1141 gaggaaaaca tttttctgga gaatcctacc tctgcagcgg agcccttaag cgattgattt
     1201 tgaatcttga ccctttacca actaattttg aagaagatac cttggaaata tttggcattc
     1261 agtgggttac tgaaacagca ttagtgaatt catctagaga actctttcat ttattcaggc
     1321 aacaactgta caacttggaa accttgttac agtccagttg tgattttggg aaggtatcaa
     1381 ctctacactg caaagcagac aatattaggc agcagtgtgt actatttctc cattatgtta
     1441 aagttttcat cttcaggtat ctgaaagtac agaatgctga gagtcatgtt cctgtccatc
     1501 cttatgaggc tttggaggct cagcttccct cagtgttgat tgatgagctt catggattac
     1561 tcttgtatat tggacaccta tctgaacttc ccagtgttaa tataggagca tttgtaaatc
     1621 aaaaccagat taagcttttt ccaccgtcat ggcatttatt acatctccac ttggatatac
     1681 attggctggt gctagaaatt ctttacatgc tgggtgaaaa attgaaacaa gttgtatatg
     1741 gtcatcagtt tatgaatctg gcaagtgaca atttaaccaa catcagccta tttgaagaac
     1801 attgtgaaac tctcctttgt gatttaataa gcctgtcact caacaggtac gacaaggtta
     1861 ggtcttctga atcattaatg agtgaccagt gtccatgttt atgcattaaa gaattatggg
     1921 ttctacttat tcatcttcta gaccacagaa gtaaatggtt tgtctcggaa tcattttgga
     1981 actggttgaa taaactactt aaaacactgc ttgaaaaatc aagtgaccga agaagatcct
     2041 ctatgcctgt aatccagtcc agggatccat taggttttag ttggtggatt attactcatg
     2101 tagcatcatt ttacaagttt gatcgccatg gagtaccaga tgaaatgaga aaagtggaat
     2161 caaattggaa ctttgtagaa gaactgctga aaaagtccat cagtgttcag ggtgtcattc
     2221 tagaagaaca attacgaatg tatcttcact gttgtttgac actttgtgat ttctgggagc
     2281 caaacattgc aattgttacc attttatggg aatattatag taagaacctg aatagttcct
     2341 tcagtatttc ttggcttcct tttaaaggcc ttgctaatac catgaagtca cccttgtcta
     2401 tgcttgaaat ggtgaaAacG tgTtgCtgTg aCaaacaaga tcaggaacta tataaatcca
     2461 gcagtagtta tactattttt ctttgtattc tggcaaaagt tgttaaaaaa gcaatgaaga
     2521 gcaatggccc tcatccttgg aaacaagtca aaggaagaat atattcaaaa ttccatcaaa
     2581 aaagaatgga agaactaact gaagttggtc tacagaactt ttttagcctt tttctactgt
     2641 tagcagctgt tgcagaggta gaagatgttg caagtcatgt tttagacctc ctgaatttcc
     2701 tcaagcctgc ttttgtaacg tctcagagag ccctcatttg gaagggtcac atggccttcc
     2761 tcttgatgta tgcccagaaa aatctggaca ttggtgtttt ggctgagaaa ttttcatgtg
     2821 ctttccggga gaaagcaaag gaattcttgg tgtctaagaa tgaggaaatg gtacagagac
     2881 agactatctg gacccttctt tccatataca ttgatggtgt tcaagaagtg tttgagacca
     2941 gctattgctt gtatccttcc catgaaaaac tgcttaatga tggatttagt atgcttctgc
     3001 gagcatgtcg agaatctgaa cttaggacag tattgagctt cctacaagct gttctggcca
     3061 gaatcaggag tatgcatcaa caattgtgtc aggaacttca aagggacaat gtggacctat
     3121 ttgtacagtc ttcattatcg gctaaagagc gccaccttgc tgcagttgcc agtgcactgt
     3181 ggagacattt cttttcattt ttgaagagtc agagaatgtc acaggtagtg cctttctcac
     3241 aacttgcgga tgcagctgca gactttactt tgctagcaat ggacatgcca agcacagctc
     3301 catcagattt tcagcctcag ccagttatat caattattca actttttggt tgggatgata
     3361 tcatctgccc tcaagttgta gcaagatatt taagtcatgt cctacaaaat agcacattat
     3421 gtgaagcact ttctcattca ggctatgtat cttttcaagc cttaaccgta agatcatgga
     3481 ttcgttgtgt tttgcaaatg tatattaaaa acctctctgg gcctgatgat ttgctcatag
     3541 ataaaaatct ggaagaggca gttgaaaaag agtacatgaa acagttggtc aaactgacaa
     3601 gattactatt taatctctca gaagtaaaga gtattttctc aaaggcccaa gttgaatatt
     3661 tatccatctc agaagaccct aaaaaagcac ttgttcgatt ctttgaggct gttggtgtaa
     3721 cttacgggaa cgtccagaca ctttctgata aatctgccat ggtcacaaag tccttggaat
     3781 accttggtga agtattaaaa tatattaagc cttatttggg aaaaaaagtt ttcagtgcag
     3841 ggctgcagct gacttatgga atgatgggaa ttcttgtgaa atcatgggca caaatctttg
     3901 ccacttctaa agcccaaaaa ttactattcc ggatcataga ttgtttactg ctgccacatg
     3961 cagtattaca gcaagagaag gaactgcctg cacctatgtt gtcagcaatt cagaaaagtc
     4021 ttcctttgta tctccagggc atgtgtatcg tgtgttgtca atctcaaaat ccgaatgcct
     4081 atttgaatca attgctaggg aatgttattg agcagtatat tgggcgattt cttccagctt
     4141 caccatatgt ttcagatctt ggacaacatc ctgttttgct ggcattgaga aacacagcca
     4201 ctattccacc aatatcatct ctaaagaaat gcattgtgca agtcataagg aaatcctacc
     4261 ttgagtataa ggggtcctca cctcctcctc gcttagcatc cattctggcc ttcatcctcc
     4321 aactcttcaa ggaaactaac acagacattt atgaagttga actactcctc cctggcattt
     4381 taaaatgctt ggtgttagtc agtgaaccac aagttaaaag gctggccaca gagaacctgc
     4441 aatacatggt aaaagcctgc caagtggggt cagaagaaga accttcctcc cagctgactt
     4501 ctgtgtttag gcagtttatc caggattatg gtatgaggta ctattaccag gtttacagca
     4561 ttttagaaac agtagcaaca ttggaccagc aggttgtcat ccacttgatt tctaccctta
     4621 ctcagtctct gaaggattca gagcagaaat ggggccttgg caggaatata gcacaaaggg
     4681 aagcctatag caaacttttg tctcaccttg gacagatggg acaagatgag atgcagagac
     4741 tggaaaatga taatactacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     4801 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     4861 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     4921 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     4981 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     5041 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     5101 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     5161 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     5221 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     5281 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     5341 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     5401 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     5461 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     5521 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5581 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5641 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5701 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5761 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5821 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5881 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5941 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6001 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6061 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6121 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6181 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6241 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6301 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     6421 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     6481 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     6541 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     6601 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     6661 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     6721 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     6781 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     6841 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     6901 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     6961 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     7021 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     7081 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     7141 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     7201 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     7261 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     7321 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     7381 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     7441 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     7501 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     7561 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     7621 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     7681 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     7741 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     7801 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     7861 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     7921 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     7981 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     8041 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8161 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8581 ctgatttaac aaaaatttaa cgcgaatttt
//

