LOCUS       polyE67R_blue           8352 bp    DNA     circular SYN 23-APR-2025
DEFINITION  polyE67R_blue.
ACCESSION   polyE67R_blue
VERSION     polyE67R_blue
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(7729..8242)
                     /note="pLannotate"
                     /label="M13 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3254..4354
                     /note="pLannotate"
                     /label="MBP"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             1807..2889
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        6735..6826
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      6511..6597
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             6827..7687
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        1729..1806
                     /note="pLannotate"
                     /label="lacIq promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        3132..3160
                     /note="pLannotate"
                     /label="tac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      6689..6716
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             4427..4447
                     /note="pLannotate"
                     /label="TEV site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6095..6115
                     /note="pLannotate"
                     /label="TEV site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1042..1233)
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    3168..3184
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             6145..6299
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     CDS             5783..6067
                     /note="pLannotate"
                     /label="SUMO1 (fragment)"
                     /database="swissprot"
                     /identity="98.9"
                     /match_length="94.1"
                     /fragment="True"
                     /other="CDS"
     CDS             5456..5740
                     /note="pLannotate"
                     /label="SUMO1 (fragment)"
                     /database="swissprot"
                     /identity="98.9"
                     /match_length="94.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(842..940)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="70.2"
                     /fragment="True"
                     /other="misc_feature"
     CDS             4469..4756
                     /note="pLannotate"
                     /label="pmt3 (fragment)"
                     /database="swissprot"
                     /identity="51.0"
                     /match_length="82.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(775..844)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="49.6"
                     /fragment="True"
                     /other="misc_feature"
     ncRNA           6392..6507
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="97.5"
                     /fragment="False"
                     /other="ncRNA"
     CDS             1250..1630
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
     protein_bind    2902..2923
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        2942..2959
                     /note="pLannotate"
                     /label="tet promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="62.1"
                     /fragment="True"
                     /other="promoter"
     CDS             6116..6132
                     /note="pLannotate"
                     /label="capTEV™ (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatgtggt gcactctcag
      841 tacaccgcat gtggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
      901 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
      961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     1081 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc
     1141 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     1201 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     1261 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     1321 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     1381 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     1441 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     1501 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     1561 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     1621 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     1681 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gaaattccga caccatcgaa
     1741 tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg
     1801 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag
     1861 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg
     1921 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc
     1981 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa
     2041 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg
     2101 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc
     2161 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct
     2221 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt
     2281 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt
     2341 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg
     2401 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc
     2461 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt
     2521 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc
     2581 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac
     2641 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa
     2701 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
     2761 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct
     2821 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2881 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac aattctcatg
     2941 tttgacagct tatcatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg
     3001 gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg
     3061 cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct
     3121 gaaatgagct gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata
     3181 acaatttcac acaggaaaca gccagtccgt ttaggtgttt tcacgagcac ttcaccaaca
     3241 aggaccatag attatgaaaa ctgaagaagg taaactggta atctggatta acggcgataa
     3301 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
     3361 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
     3421 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
     3481 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
     3541 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
     3601 gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
     3661 ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta
     3721 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
     3781 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
     3841 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
     3901 tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat
     3961 cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc
     4021 caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct
     4081 ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa
     4141 agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc
     4201 acgtattgcc gccaccatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca
     4261 gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca
     4321 gactgtcgat gaagccctga aagacgcgca gactaattcg agctcgaaca acaacaacaa
     4381 taacaataac aacaacctcg ggatcgaggg aaggatttca gaatttgaaa acctgtattt
     4441 tcagggccat atgggaggcg gctcaggtgg ttccatgagc gatcaggaag cgaaaccgag
     4501 caccgaagat ctgggcgata aaaaagaagg cgaatatatt aaactgaaag tgattggcca
     4561 ggatagcagc gaaattcatt ttaaagtgaa aatgaccacc catctgaaaa aactgaaaga
     4621 aagctattgc cagcgccagg gcgtgccgat gaacagcctg cgctttctgt ttcgcggcca
     4681 gcgcattgcg gataaccata ccccgaaaga actgggcatg gaagaagaag atgtgattga
     4741 agtgtatcag gaacagaccg tggtgggcgg cagcggcggc agcggcggca gcggcggcag
     4801 catgagcgat caggaagcga aaccgagcac cgaagatctg ggcgataaaa aagaaggcga
     4861 atatattaaa ctgaaagtga ttggccagga tagcagcgaa attcatttta aagtgaaaat
     4921 gaccacccat ctgaaaaaac tgaaagaaag ctattgccag cgccagggcg tgccgatgaa
     4981 cagcctgcgc tttctgtttc gcggccagcg cattgcggat aaccataccc cgaaagaact
     5041 gggcatggaa gaagaagatg tgattgaagt gtatcaggaa cagaccgtgg tgggcggcag
     5101 cggcggcagc ggcggcagcg gcggcagcat gagcgatcag gaagcgaaac cgagcaccga
     5161 agatctgggc gataaaaaag aaggcgaata tattaaactg aaagtgattg gccaggatag
     5221 cagcgaaatt cattttaaag tgaaaatgac cacccatctg aaaaaactga aagaaagcta
     5281 ttgccagcgc cagggcgtgc cgatgaacag cctgcgcttt ctgtttcgcg gccagcgcat
     5341 tgcggataac cataccccga aagaactggg catggaagaa gaagatgtga ttgaagtgta
     5401 tcaggaacag accgtggtgg gcggcagcgg cggcagcggc ggcagcggcg gcagcatgag
     5461 cgatcaggaa gcgaaaccga gcaccgaaga tctgggcgat aaaaaagaag gcgaatatat
     5521 taaactgaaa gtgattggcc aggatagcag cgaaattcat tttaaagtga aaatgaccac
     5581 ccatctgaaa aaactgaaag aaagctattg ccagcgccag ggcgtgccga tgaacagcct
     5641 gcgctttctg tttcgcggcc agcgcattgc ggataaccat accccgaaag aactgggcat
     5701 ggaagaagaa gatgtgattg aagtgtatca ggaacagacc gtggtgggcg gcagcggcgg
     5761 cagcggcggc agcggcggca gcatgagcga tcaggaagcg aaaccgagca ccgaagatct
     5821 gggcgataaa aaagaaggcg aatatattaa actgaaagtg attggccagg atagcagcga
     5881 aattcatttt aaagtgaaaa tgaccaccca tctgaaaaaa ctgaaagaaa gctattgcca
     5941 gcgccagggc gtgccgatga acagcctgcg ctttctgttt cgcggccagc gcattgcgga
     6001 taaccatacc ccgaaagaac tgggcatgga agaagaagat gtgattgaag tgtatcagga
     6061 acagaccgtg gtaggcggct cctgtggtgg atccgaaaac ctgtattttc agggccatca
     6121 tcatcatcat cactgaaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6181 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6241 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6301 ggcagcttgg ctgttttggc ggatgagata agattttcag cctgatacag attaaatcag
     6361 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     6421 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     6481 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     6541 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     6601 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     6661 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     6721 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6781 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6841 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6901 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6961 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgtttccc
     7021 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     7081 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7141 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7201 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7261 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7321 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7381 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7441 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7501 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7561 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7621 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7681 ttggtaactg tcagaccaag tttactcata tatactttag attgatttac cccggttgat
     7741 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaaacgtt
     7801 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     7861 gccgaaatcg gcaaaatccc ttataaatca aaagaatagc ccgagatagg gttgagtgtt
     7921 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     7981 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg
     8041 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     8101 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     8161 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     8221 aatgcgccgc tacagggcgc gtaaaaggat ctaggtgaag atcctttttg ataatctcat
     8281 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8341 caaaggatct tc
//
