LOCUS       Exported               11935 bp ds-DNA     circular UNK 09-MAY-2024
DEFINITION  natural circular DNA
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 9, 2024 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     rep_origin      665..785
                     /label=RSF ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=95.041"
                     /note="/match_length=16.133333333333333"
                     /note="/other=rep_origin"
     CDS             786..840
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.078485687903971"
                     /note="/other=CDS"
     promoter        complement(856..953)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=96.07843137254902"
                     /note="/other=promoter"
     promoter        1066..1096
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(1100..1122)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
     protein_bind    1104..1120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1150..1453
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        1454..1657
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     1633..1657
                     /label=p121_F_5UTR_SEMPERmARG_HA_pCMV
     misc_feature    1671..1744
                     /label=5CMV UTR 74bp
     primer_bind     1671..1689
                     /label=p107_F_5CMV_74bp_HA_T7
     primer_bind     1723..1762
                     /label=p128_F_ACC-GvpA_HA_CMV173
     primer_bind     1723..1757
                     /label=p105_F_ACC_gvpA_HA_5cmv173
     primer_bind     complement(1725..1744)
                     /label=p161_R_insC_TIS1
     misc_feature    1742..1752
                     /label=TIS
     primer_bind     1744..1762
                     /label=p160_F_insC_TIS1
     misc_feature    1745..1751
                     /label=ACC TIS
     CDS             1748..1963
                     /codon_start=1
                     /label=GvpA
                     /translation="MAVEKTNSSSSLAEVIDRILDKGIVIDAWVRVSLVGIELLAIEAR
                     IVIASVETYLKYAEAVGLTQSAAVPA"
     primer_bind     1946..1983
                     /label=p136_F_GvpN_HA_GvpA3pfix_SmaI
     primer_bind     complement(1946..1963)
                     /label=p129_R_GvpA_HA_AfIII
     primer_bind     complement(1954..1974)
                     /label=p139_R_GvpA-ttaccatggtg
     primer_bind     1964..1983
                     /label=p132_F_GvpNWEm-HA-SmaI-GvpA-TC
     primer_bind     1964..1983
                     /label=p_F_GvpNWEm-HA-SmaI-GvpA3fix
     misc_feature    1966..1972
                     /label=ACC TIS
     CDS             1969..2976
                     /codon_start=1
                     /gene="gvpN"
                     /product="Important for elongation"
                     /label=gvpN
                     /note="putative gas vesicle protein N"
                     /protein_id="AAA58711.1"
                     /translation="MVTTTKVNHKRAVLRLRPGQFVVTPAIERVAIRALRYLKSGFPVH
                     LRGPAGTGKTTLAMHLANCLDRPVMLLFGDDQFKSSDLIGSESGYTHKKVLDNYIHSVV
                     KLEDEFKQNWVDSRLTLACREGFTLVYDEFNRSRPEVNNVLLSALEEKILSLPPSSNQP
                     EYLSVNPQFRVIFTSNPEEYAGVHSTQDALMDRLVTISMPEPDEITQTEILIQKTNIDR
                     ESANFIVRLVKSFRLATGAEKTSGLRSCLMIAKVCADNNIPVTTESLDFPDIAIDILFN
                     RSHLSMSESTNIFLELLDKFSAEELEILNNRVTGDNDFLIDNSQFVSQQLAGQPN"
     primer_bind     complement(2976..3032)
                     /label=R_linker_p2a_long
     CDS             2977..2994
                     /codon_start=1
                     /label=linker_1
                     /translation="GAPGSG"
     CDS             2995..3051
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3028..3051
                     /label=T2A
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=95.833"
                     /note="/match_length=44.44444444444444"
                     /note="/other=CDS"
     primer_bind     complement(3034..3057)
                     /label=p137_R_P2A_1_HA_GvpG
     CDS             3052..3795
                     /codon_start=1
                     /label=GvpJ
                     /translation="VLPTRPQTNSSRTINTSTQGSTLADILERVLDKGIVIAGDISISI
                     ASTELVHIRIRLLISSVDKAKEMGINWWESDPYLSTKAQRLVEENQQLQHRLESLEAKL
                     NSLTSSSVKEEIPLAADVKDDLYQTSAKIPSPVDTPIEVLDFQAQSSGGTPPYVNTSME
                     ILDFQAQTSAESSSPVGSTVEILDFQAQTSEESSSPVVSTVEILDFQAQTSEESSSPVG
                     STVEILDFQAQTSEEIPSSVDPAIDV"
     primer_bind     3052..3069
                     /label=p140_F_GvpJ_HA_GvpA-ACCatggtg
     CDS             3796..3813
                     /codon_start=1
                     /label=Linker_2
                     /translation="GAPGSG"
     CDS             3814..3870
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_2
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3833..3842
                     /label=p139_R_GvpA-ttaccatggtg
     CDS             3835..3857
                     /label=P2A
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=95.652"
                     /note="/match_length=40.35087719298245"
                     /note="/other=CDS"
     primer_bind     complement(3853..3873)
                     /label=R_P2A_1V_HA_mCherry
     primer_bind     complement(3853..3873)
                     /label=p158_R_GvpW_p2a_HA_NLS
     CDS             3871..4335
                     /codon_start=1
                     /label=GvpK
                     /translation="VVCTPAENFNNSLTIASKPKNEAGLAPLLLTVLELVRQLMEAQVI
                     RRMEEDLLSEPDLERAADSLQKLEEQILHLCEMFEVDPADLNINLGEIGTLLPSSGSYY
                     PGQPSSRPSVLELLDRLLNTGIVVDGEIDLGIAQIDLIHAKLRLVLTSKPI"
     CDS             4336..4353
                     /codon_start=1
                     /label=Linker_3
                     /translation="GAPGSG"
     CDS             4354..4410
                     /label=P2A
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.491"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     primer_bind     complement(4387..4413)
                     /label=p141_R_P2AafterF_HA_EBFP2
     CDS             4411..5145
                     /codon_start=1
                     /label=GvpF
                     /translation="VSIPLYLYGIFPNTIPETLELEGLDKQPVHSQVVDEFCFLYSEAR
                     QEKYLASRRNLLTHEKVLEQTMHAGFRVLLPLRFGLVVKDWETIMSQLINPHKDQLNQL
                     FQKLAGKREVSIKIFWDAKAELQTMMESHQDLKQQRDNMEGKKLSMEEVIQIGQLIEIN
                     LLARKQAVIEVFSQELNPFAQEIVVSDPMTEEMIYNAAFLIPWESESEFSERVEVIDQK
                     FGDRLRIRYNNFTAPYTFAQLDS"
     CDS             5146..5163
                     /codon_start=1
                     /label=Linker_4
                     /translation="GAPGSG"
     CDS             5164..5220
                     /label=P2A
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.491"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     primer_bind     complement(5197..5223)
                     /label=p141_R_P2AafterF_HA_EBFP2
     CDS             5221..5577
                     /codon_start=1
                     /label=GvpG
                     /translation="VLTKLLLLPIMGPLNGVVWIAEQIQERTNTEFDAQENLHKQLLSL
                     QLSFDIGEIGEEEFEIQEEEILLKIQALEEEARLELEAEQEEARLELEAEQEDFEYPPQ
                     FTAEVNKDQHLVLLP"
     primer_bind     5221..5241
                     /label=p138_F_GvpG
     CDS             5578..5595
                     /codon_start=1
                     /label=Linker_2
                     /translation="GAPGSG"
     CDS             5596..5652
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5629..5652
                     /label=T2A
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=95.833"
                     /note="/match_length=44.44444444444444"
                     /note="/other=CDS"
     primer_bind     complement(5635..5655)
                     /label=p137_R_P2A_1_HA_GvpG
     CDS             5653..6330
                     /codon_start=1
                     /label=GvpW
                     /translation="VELENLYTYAFLEIPSSPLILPQGAANQVVLINGTELAAIVEPGI
                     FLESFQNNDEKIIQMALSHDRVICELFQQITVLPLRFGTYFTSTNNLLNHLKSHEKEYQ
                     NKLEKINGKNEFTLKLIPRMIEEIVPSEGGGKDYFLAKKQRYQNQNNFSIAQAAEKQNL
                     IDLITKVNQLPVVVQEQEEQIQIYLLVSCQDKTLLLEQFLTWQKACPKWDLLLGDCLPP
                     YHFI"
     primer_bind     5700..5717
                     /label=p157_F_internal_GvpW
     CDS             5925..5943
                     /label=AsCpf1
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=0.4845702626880897"
                     /note="/other=CDS"
     primer_bind     6309..6330
                     /label=p_F_linker_P2A_HA_GvpW
     primer_bind     complement(6330..6389)
                     /label=R_linker_p2a_long
     CDS             6331..6348
                     /codon_start=1
                     /label=linker_1
                     /translation="GAPGSG"
     CDS             6349..6405
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A_2
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     6368..6377
                     /label=p139_R_GvpA-ttaccatggtg
     CDS             6370..6392
                     /label=P2A
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=95.652"
                     /note="/match_length=40.35087719298245"
                     /note="/other=CDS"
     primer_bind     complement(6388..6408)
                     /label=R_P2A_1V_HA_mCherry
     primer_bind     complement(6388..6408)
                     /label=p158_R_GvpW_p2a_HA_NLS
     primer_bind     6393..6427
                     /label=p120_F_ACC_EmGFP_HA_5CMV173_v2
     CDS             6406..7125
                     /codon_start=1
                     /product="Emerald is a basic (constitutively fluorescent) 
                     green fluorescent protein published in 1998, derived from 
                     Aequorea victoria. It is reported to be a very 
                     rapidly-maturing weak dimer."
                     /label=EmGFP
                     /translation="VVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     6407..6427
                     /label=p111_F_ACC_EmGFP_HA_5CMV173
     primer_bind     complement(6408..6426)
                     /label=p122_R_P2A_linker_HA_mCherryrecode
     CDS             6409..7121
                     /label=Emerald
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.581589958159"
                     /note="/other=CDS"
     primer_bind     complement(7107..7132)
                     /label=p133_R_EmGFP-XhoI-BB
     misc_feature    7130..7150
                     /label=random
     primer_bind     complement(7131..7149)
                     /label=p108_R_EmGFPrand_HA_XhoI
     primer_bind     complement(7131..7149)
                     /label=p106_R_EmGFP_HA_randWPRE
     misc_feature    7151..7722
                     /label=IRES2
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.825"
                     /note="/match_length=97.7815699658703"
                     /note="/other=misc feature"
     misc_feature    7160..7711
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     7571..7591
                     /label=H2seqF
     primer_bind     complement(7672..7692)
                     /label=H1seqR
     CDS             7711..7722
                     /codon_start=1
                     /label=IRES
                     /translation="MATT"
     CDS             7723..8434
                     /label=mCherry
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.719"
                     /note="/match_length=100.14064697609003"
                     /note="/other=CDS"
     primer_bind     complement(7878..7891)
                     /label=p122_R_P2A_linker_HA_mCherryrecode
     primer_bind     complement(8416..8463)
                     /label=p133_R_EmGFP-XhoI-BB
     promoter        complement(8489..8507)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          8521..8635
                     /label=large T intron
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=33.23699421965318"
                     /note="/other=intron"
     intron          complement(8705..8734)
                     /label=small t intron
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=96.667"
                     /note="/match_length=45.45454545454545"
                     /note="/other=intron"
     CDS             8734..8871
                     /label=large T antigen
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.497175141242938"
                     /note="/other=CDS"
     primer_bind     8893..8914
                     /label=p125_F_WPRE_HA_MS28x_MCS
     misc_feature    8894..8921
                     /label=F4
     primer_bind     8894..8914
                     /label=p109_F_randWPRE
     misc_feature    8894..8901
                     /label=random
     misc_feature    complement(8902..9490)
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.831"
                     /note="/match_length=100.0"
                     /note="/other=misc feature"
     primer_bind     complement(8924..8956)
                     /label=WPREseqR
     CDS             complement(9373..9384)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9487..9510)
                     /label=p126_R_randWPRE_HA_cargo
     primer_bind     complement(9487..9509)
                     /label=p110_R_randWPRE_HA_XHOI
     misc_feature    9491..9509
                     /label=random
     polyA_signal    9510..9986
                     /label=hGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA signal"
     misc_feature    9987..9996
                     /label=random
     CDS             complement(10001..10050)
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.62962962962963"
                     /note="/other=CDS"
     CDS             10050..10120
                     /label=lacZ
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=2.333990795529257"
                     /note="/other=CDS"
     rep_origin      10122..10576
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.781"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        10602..10658
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=55.88235294117647"
                     /note="/other=promoter"
     promoter        10811..10862
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=72.22222222222221"
                     /note="/other=promoter"
     CDS             10863..11723
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.884"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     protein_bind    complement(11825..11858)
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=97.143"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttattcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      841 agcttgcaat tcgcgctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      901 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      961 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1021 aataggcgta tcacgaggcc ctttcactca ttaggcaccc caggctttac actttatgct
     1081 tccggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcat
     1141 cgtgcaggtc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     1201 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     1261 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     1321 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     1381 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     1441 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     1501 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1561 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1621 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     1681 ctggagacgc catccacgct gttttgacct ccatagaaga ccgagctcgg atccagtacc
     1741 cttcaccatg gccgtggaaa agaccaacag cagcagctcc ctggccgaag tgatcgacag
     1801 aatcctggac aagggcatcg tgatcgacgc ctgggtgcgc gtgtccctcg tgggaattga
     1861 gctgctggcc atcgaggccc ggatcgtgat tgccagcgtg gaaacctacc tgaagtacgc
     1921 cgaggccgtg ggcctgacac agagtgctgc tgtgcctgct tgattaccat ggtgaccacc
     1981 accaaagtga accacaagcg ggccgtgctg agactgaggc ctggccagtt tgtcgtgacc
     2041 cccgccattg agcgggtggc cattagagcc ctgagatacc tgaagtccgg cttccccgtg
     2101 cacctgagag gacctgccgg aaccggcaag accacactgg ccatgcacct ggccaactgc
     2161 ctggacagac ccgtgatgct gctgttcggc gacgaccagt tcaagagcag cgacctgatc
     2221 ggcagcgaga gcggctacac ccacaagaag gtgctggaca actacatcca cagcgtcgtg
     2281 aagctggaag atgagttcaa gcagaactgg gtggacagca gactgaccct ggcctgccgg
     2341 gaaggcttca ccctggtgta cgacgagttc aaccggtcca gacccgaagt gaacaacgtg
     2401 ctgctgagcg ccctggaaga gaagatcctg agcctgcccc ccagcagcaa ccagccagag
     2461 tacctgagcg tgaaccccca gttcagagtg atcttcacca gcaaccccga ggaatatgcc
     2521 ggcgtgcaca gcacccagga cgccctgatg gaccggctcg tgaccatctc catgcccgag
     2581 cccgatgaga tcacccagac cgagatcctg atccagaaaa caaacatcga ccgcgagagc
     2641 gccaacttca tcgtgcggct cgtgaagtcc ttcagactgg ccacaggcgc cgagaaaacc
     2701 agcggcctga gaagctgcct gatgatcgcc aaagtgtgcg ccgacaacaa catccccgtg
     2761 accaccgaga gcctggactt ccccgatatc gccatcgaca tcctgttcaa ccgcagccac
     2821 ctgagcatga gcgagtccac caacatcttt ctggaactgc tggataagtt cagcgccgag
     2881 gaactggaaa tcctgaacaa cagagtgacc ggcgacaacg acttcctgat cgacaacagc
     2941 cagttcgtgt cccagcagct ggccggacag cccaacggag cgccaggttc cggggctact
     3001 aacttcagcc tccttaaaca ggctggcgat gtggaagaaa atcccggacc agtgctgccc
     3061 accagacccc agaccaacag cagccggacc atcaacacca gcacccaggg cagcaccctg
     3121 gccgacatcc tggaaagagt gctggacaag ggcatcgtga tcgccgggga catcagcatc
     3181 tctatcgcca gcaccgagct ggtgcacatc cggatcagac tgctgatctc cagcgtggac
     3241 aaggccaaag agatgggcat caactggtgg gagagcgacc cctacctgag caccaaggcc
     3301 cagagactgg tggaagagaa ccagcagctc cagcacagac tggaaagcct ggaagccaag
     3361 ctgaacagcc tgaccagcag cagcgtgaaa gaggaaatcc ccctggccgc cgacgtgaag
     3421 gacgacctgt atcagaccag cgccaagatc cccagccccg tggatacccc tatcgaggtg
     3481 ctggacttcc aggcccagtc tagcggcggc acaccccctt acgtgaacac ctctatggaa
     3541 atactggatt ttcaggctca gacctccgcc gagagcagca gccctgtggg ctctaccgtg
     3601 gaaatactgg acttccaggc acagacaagc gaggaatcca gctcccccgt ggtgtccaca
     3661 gtggaaatac tggatttcca ggcccagact tctgaagagt ccagctctcc agtgggaagc
     3721 actgtggaaa tactggactt tcaggcccag accagtgaag agatccccag ctctgtggac
     3781 cccgccatcg atgtgggtgc cccgggatct ggcgcaacaa attttagtct cctcaagcag
     3841 gcaggagatg tcgaggaaaa ccctggaccc gtggtgtgca cccctgccga gaacttcaac
     3901 aacagcctga caatcgccag caagcccaag aacgaggccg gactggctcc tctgctgctg
     3961 accgtgctgg aactcgtgcg gcagctgatg gaagcccaag tgatccggcg gatggaagag
     4021 gacctgctga gcgagcccga cctggaaaga gccgccgata gcctccagaa gctggaagaa
     4081 cagatcctgc acctgtgcga gatgttcgag gtggaccccg ccgacctgaa catcaacctg
     4141 ggcgagatcg gcaccctgct gcctagcagc ggcagctact atcctggcca gccaagcagc
     4201 agaccctctg tgctggaact gctggaccgg ctgctgaaca ccggcatcgt ggtggacggc
     4261 gagattgacc tgggaatcgc ccagatcgac ctgatccacg ccaagctgag actggtgctg
     4321 acctccaagc ccatcggggc acctggctca ggagcgacca acttctcatt gctcaaacaa
     4381 gccggagatg ttgaggagaa tccaggccct gtgagcatcc ccctgtacct gtacggcatc
     4441 ttccccaaca ccatccccga gacactggaa ctggaaggcc tggacaagca gcccgtgcac
     4501 agccaggtgg tggacgagtt ctgcttcctg tacagcgagg cccggcagga aaagtacctg
     4561 gccagcagac ggaacctgct gacccacgag aaggtgctgg aacagaccat gcacgccggc
     4621 ttcagagtgc tgctgcccct gagatttggc ctggtcgtga aggactggga gacaatcatg
     4681 agccagctga tcaaccccca caaggaccag ctgaaccagc tgttccagaa gctggccggc
     4741 aagcgggaag tgtccatcaa gattttctgg gacgccaagg ccgagcttca gaccatgatg
     4801 gaaagccacc aggacctgaa gcagcagcgg gacaacatgg aaggcaagaa actgagcatg
     4861 gaagaagtga tccagatcgg gcagctgatc gagatcaacc tgctggcccg gaagcaggcc
     4921 gtgatcgagg tgttcagcca ggaactgaac cccttcgccc aggaaatcgt ggtgtccgac
     4981 cccatgaccg aggaaatgat ctacaacgcc gccttcctga tcccctggga gagcgagagc
     5041 gagttcagcg agcgggtgga agtgatcgac cagaagttcg gcgaccggct gcggatcaga
     5101 tacaacaact tcaccgcccc ctacaccttt gcccagctgg actctggcgc tcccgggtca
     5161 ggtgccacta atttttcatt gttgaagcaa gctggggatg ttgaagagaa cccagggcct
     5221 gtgctgacca aactcctgct gctgcccatc atgggacccc tgaatggcgt cgtgtggatc
     5281 gccgagcaga tccaggaacg gaccaacacc gagttcgacg cccaggaaaa cctgcacaaa
     5341 cagctgctga gcctccagct gagcttcgac atcggcgaga tcggagagga agagttcgag
     5401 attcaggaag aggaaatcct gctgaagatc caggccctgg aagaagaggc ccggctggaa
     5461 ctggaagccg agcaggaaga agccagactg gaactggaag ctgaacagga agatttcgag
     5521 tacccaccac agttcaccgc cgaagtgaac aaggatcagc atctggtgct gctgcccggt
     5581 gccccgggat ctggcgctac taacttcagc ctccttaaac aggctggcga tgtggaagaa
     5641 aatcccggac cagtggaact ggaaaacctg tacacctacg cctttctgga aatccccagc
     5701 agccccctga tcctgcctca gggcgctgct aatcaggtgg tgctgatcaa cggcaccgag
     5761 ctggccgcca tcgtggaacc tggcatcttc ctggaatcct tccagaacaa cgacgagaag
     5821 atcatccaga tggccctgag ccacgacaga gtgatctgcg agctgttcca gcagatcacc
     5881 gtgctgcccc tgagattcgg cacctacttc accagcacca acaacctgct gaaccacctg
     5941 aagtcccacg agaaagagta ccagaacaag ctggaaaaga tcaacgggaa gaacgagttc
     6001 accctgaagc tgatcccccg gatgatcgag gaaatcgtgc cctctgaggg cggaggcaag
     6061 gactacttcc tggccaagaa gcagcgctac cagaatcaga acaacttctc tatcgcccag
     6121 gccgccgaga agcagaacct gatcgacctg atcaccaaag tgaaccagct gcccgtggtg
     6181 gtgcaggaac aggaagaaca gatccaaatc tacctgctgg tgtcctgcca ggataagacc
     6241 ctgctgctgg aacagttcct gacctggcag aaagcctgcc ccaagtggga tctgctgctg
     6301 ggcgactgcc tgccccccta ccactttatc ggagcgccag gttccggggc aacaaatttt
     6361 agtctcctca agcaggcagg agatgtcgag gaaaaccctg gacccgtggt gtccaagggc
     6421 gaggaactgt tcaccggcgt ggtgcccatc ctggtggaac tggatggcga cgtgaacggc
     6481 cacaagttca gcgtgtccgg cgagggcgaa ggcgacgcca catacggaaa gctgaccctg
     6541 aagttcatct gcaccaccgg caagctgccc gtgccttggc ctaccctcgt gaccacactg
     6601 acctacggcg tgcagtgctt cgccagatac cccgaccaca tgaagcagca cgatttcttc
     6661 aagagcgcca tgcccgaggg ctacgtgcag gaacggacca tcttcttcaa ggacgacggc
     6721 aactacaaga caagagccga agtgaagttc gagggcgaca ccctcgtgaa ccggatcgag
     6781 ctgaagggca tcgacttcaa agaggatggc aacatcctgg gccacaagct ggagtacaac
     6841 tacaacagcc acaaggtgta catcaccgcc gacaagcaga aaaacggcat caaagtgaac
     6901 ttcaagaccc ggcacaacat cgaggacggc agcgtgcagc tggccgacca ctaccagcag
     6961 aacaccccca tcggagatgg ccccgtgctg ctgcccgaca accactacct gagcacacaa
     7021 agcgccctga gcaaggaccc caacgagaag cgggaccaca tggtgctgct ggaatttgtg
     7081 accgccgctg gcatcaccct gggcatggac gagctgtaca agtgataata ctaaatcgca
     7141 ctgtcggcgt ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     7201 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     7261 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     7321 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     7381 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     7441 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     7501 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     7561 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     7621 tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg
     7681 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag
     7741 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     7801 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     7861 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgccct gggacatcct
     7921 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     7981 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     8041 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     8101 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     8161 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     8221 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     8281 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     8341 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     8401 ctccaccggc ggcatggatg agctgtacaa gtgaaggatc cctcgagggg cccaagctta
     8461 cgcgtgcatg cgacgtcata gctctctccc tatagtgagt cgtattataa gctagcttgg
     8521 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     8581 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactagctgc
     8641 atatgcttgc tgcttgagag ttttgcttac tgagtatgat ttatgaaaat attatacaca
     8701 ggagctagtg attataattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag
     8761 gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt
     8821 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     8881 aattgttgtt gttcgtggct gaatcaacct ctggattaca aaatttgtga aagattgact
     8941 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     9001 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     9061 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9121 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9181 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9241 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9301 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9361 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9421 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9481 gcctccccgc cagagacaat taacttcgcg ggtggcatcc ctgtgacccc tccccagtgc
     9541 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
     9601 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
     9661 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
     9721 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
     9781 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
     9841 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
     9901 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
     9961 gaaccactgc tcccttccct gtccttgaag ttctcagatc ctgcattaat gaatcggcca
    10021 acgcgcgggg agaggcggtt tgcgtattgg ctggcgtaat agcgaagagg cccgcaccga
    10081 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
    10141 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    10201 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    10261 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    10321 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10381 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
    10441 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10501 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10561 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10621 tatttttcta aatacattca aatatgtatc cgctcatgcc aggtcttgga ctggtgagaa
    10681 cggcttgctc ggcagcttcg atgtgtgctg gagggagaat aaaggtctaa gatgtgcgat
    10741 agagggaagt cgcattgaat tatgtgctgt gtagggatcg ctggtatcaa atatgtgtgc
    10801 ccacccctgg catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10861 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10921 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10981 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    11041 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    11101 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    11161 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    11221 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    11281 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    11341 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    11401 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    11461 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    11521 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    11581 ctcgcggtat cattgcagta ctggggccag atggtaagcc ctcccgtatc gtagttatct
    11641 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11701 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    11761 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    11821 tgccataact tcgtataatg tatgctatac gaagttatgg catgaccaaa atcccttaac
    11881 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
