LOCUS       pCB-CBEv2       16524 bp DNA     circular SYN 13-MAY-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6..33
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        52..143
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(144..212,213..1004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  144 ..  212 / #ccffcc / signal sequence
                        2:  213 .. 1004 / #ccffcc
                     Cleavage site after base 212"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1843..2814
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /note="color: #993366"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             3325..4164
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /note="color: #993366"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             4151..5002
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /note="color: #993366"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(5270..10744)
                     /label=PpAPOBEC1H122A-Cas9n-UGI-UGI (BE4-PpAPOBEC1H122A)
                     /note="color: #993366; direction: LEFT"
     CDS             complement(5279..5527)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /note="color: #993366"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(5558..5806)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /note="color: #993366"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(join(5837..9910,9911..9913,9914..9937))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /note="This feature has 3 segments:
                        1: 5837 .. 9910 / #993366
                        2: 9911 .. 9913 / #cc99b2
                        3: 9914 .. 9937 / #993366"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(10745..10810)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 10744"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    10842..10858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(10866..10872,10873..10888,10889..10894))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
                     /note="This reverse directional feature has 3 segments:
                        1: 10866 .. 10872 / #ffffff / -10
                        2: 10873 .. 10888 / #ffffff
                        3: 10889 .. 10894 / #ffffff / -35"
     promoter        11124..11201
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
                     /note="color: #ffffff; direction: RIGHT"
     CDS             11202..12284
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        12614..12808
                     /label=cbu1169 promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             12815..13474
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             join(13495..13581,13582..14916)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /note="This feature has 2 segments:
                        1: 13495 .. 13581 / #993366 / signal peptide
                        2: 13582 .. 14916 / #993366
                     Cleavage site after base 13581"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     rep_origin      15303..15697
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
                     /note="color: #ffff00"
     oriT            16039..16126
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
                     /note="color: #a6acb3"
ORIGIN
        1 tattgagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
       61 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      121 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      181 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      241 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      301 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      361 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
      421 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      481 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      541 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      601 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      661 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
      721 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
      781 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
      841 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
      901 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
      961 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaatacgct ggccatccat
     1021 gccggtggcg gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc
     1081 atcgagcggc ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc
     1141 ggggcacaga agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca
     1201 tgggccgacc atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac
     1261 agaacacttg aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg
     1321 gtggagatgg aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac
     1381 accgccaacg cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc
     1441 aatcgacaga gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct
     1501 ggcccagagc atggcgacac tggccgacga agcccggcag gtcatgagcc agacccagca
     1561 ggccagcgag gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg
     1621 ggtggagctg gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag
     1681 cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc
     1741 catgatgcct acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact
     1801 gacaaccgag gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact
     1861 ttgcaggcca taggccgaca gctcaaggcc atgggctgtg agcgcttcga tattggcgtc
     1921 agggacgcca ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag
     1981 aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc
     2041 gagcaggagc ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac
     2101 atgaaagccg agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag
     2161 gcatgggtca aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg
     2221 ctggccagcg agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg
     2281 gcgggcttca ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg
     2341 ctgctgcgtg aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct
     2401 ggccagcaga tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc
     2461 gaactgcccg agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc
     2521 gagatggccg ggctggtcaa gcgcttcggt gatgacctca gcaagtgcga ctttatcgcc
     2581 gcgcagaagc tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag
     2641 gccagcccag cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc
     2701 gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca
     2761 ccggcacccc gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg
     2821 cgttggtact cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa
     2881 tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg
     2941 cccggtggct ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat
     3001 atggccagcc ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc
     3061 ggccccggca agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga
     3121 cctgctggcc agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa
     3181 agggatgcgt cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg
     3241 cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc
     3301 aactgcctgc aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg
     3361 ttcgcagcag cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc
     3421 ggggcgctgg tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca
     3481 cagattgcag gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc
     3541 tacctgcccg ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg
     3601 cacctcagcg ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc
     3661 ggcagcctgc ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag
     3721 ggccgccgcc tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc
     3781 agcggcccca tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc
     3841 tctatcgtgt tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag
     3901 cagcaggcca gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg
     3961 tcgagcatga ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc
     4021 ttcgtccgct tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc
     4081 aggcggcatg acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag
     4141 ggggtgcccc gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc
     4201 ggcgcactgt ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag
     4261 caagctggac gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga
     4321 gccgctgggc gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa
     4381 tggcctagtg cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct
     4441 ggaacccaag tgggaggccg tcaccgctga tgccatggtg gtcaaaggta gctatcgggc
     4501 gctggcaaag gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga
     4561 ctgcatcgag cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg
     4621 gtttcggctg ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct
     4681 gaaccccttg atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga
     4741 cgaggtgcgg gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg
     4801 gatcgacccc ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc
     4861 gtcagaggcc agtggttcga ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc
     4921 ggagctggtc gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac
     4981 ccggcccaag gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt
     5041 ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
     5101 aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
     5161 ccgctttcct ggctttgctt ccagatgtat gctcttctgc tcggtctaga aagtatagga
     5221 acttcagagc gcttttgaag ctaattcgat catgcatgag ctcactagtt taaccagaga
     5281 gcattttgat tttgttttcc ccattggagt cctgaatgac aagtgcccat ggcttatact
     5341 cgggagcgtc gctggtgagg agcatgacat tctcatcggt agactcgtcg taggctgtat
     5401 ggaccagaat atcgctctca ggcttgtttc caatgacttc ctcgacttct tccgggagca
     5461 tgagaataga ctcttgaatc acgagctgtt tcccggtttc tttctcaata atatcggaca
     5521 ggtttgtaga tccgccagag ccaccagagc cgccgctcag catcttgatc ttgttctcgc
     5581 cgttgctgtc ctggatcacg agagcccaag gcttgtactc aggggcgtca gaggtcagca
     5641 gcatcacgtt ctcgtcggtg ctctcatcgt aggcggtgtg caccaggatg tcggactcgg
     5701 gcttgttgcc gatcacttct tccacctctt caggcagcat caggatggac tcttggatga
     5761 ccagctgctt gcctgtctct ttctcgatga tgtcgctcag attggtgctg ccgccagatc
     5821 ctccgcttcc accagagtca cctcccagct gagacaggtc gatccgtgtc tcgtacaggc
     5881 cggtgatgct ctggtggatc agggtggcgt ccagcacctc tttggtgctg gtgtacctct
     5941 tccggtcgat ggtggtgtca aagtacttga aggcggcagg ggctcccaga ttggtcaggg
     6001 taaacaggtg gatgatattc tcggcctgct ctctgatggg cttatcccgg tgcttgttgt
     6061 aggcggacag cactttgtcc agattagcgt cggccaggat cactctcttg gagaactcgc
     6121 tgatctgctc gatgatctcg tccaggtagt gcttgtgctg ttccacaaac agctgtttct
     6181 gctcattatc ctcgggggag cccttcagct tctcatagtg gctggccagg tacaggaagt
     6241 tcacatattt ggagggcagg gccagttcgt ttcccttctg cagttcgccg gcagaggcca
     6301 gcattctctt ccggccgttt tccagctcga acagggagta cttaggcagc ttgatgatca
     6361 ggtccttttt cacttctttg tagcccttgg cttccagaaa gtcgatggga ttcttctcga
     6421 agctgcttct ttccatgatg gtgatcccca gcagctcttt cacactcttc agtttcttgg
     6481 acttgccctt ttccactttg gccaccacca gcacagaata ggccacggtg gggctgtcga
     6541 agccgccgta cttcttaggg tcccagtcct tctttctggc gatcagctta tcgctgttcc
     6601 tcttgggcag gatagactct ttgctgaagc cgcctgtctg cacctcggtc tttttcacga
     6661 tattcacttg gggcatgctc agcactttcc gcacggtggc aaaatcccgg cccttatccc
     6721 acacgatctc cccggtttcg ccgtttgtct cgatcagagg ccgcttccgg atctcgccgt
     6781 tggccagggt aatctcggtc ttgaaaaagt tcatgatgtt gctgtagaag aagtacttgg
     6841 cggtagcctt gccgatttcc tgctcgctct tggcgatcat cttccgcacg tcgtacacct
     6901 tgtagtcgcc gtacacgaac tcgctttcca gcttagggta ctttttgatc agggcggttc
     6961 ccacgacggc gttcaggtag gcgtcgtggg cgtggtggta gttgttgatc tcgcgcactt
     7021 tgtaaaactg gaaatccttc cggaaatcgg acaccagctt ggacttcagg gtgatcactt
     7081 tcacttcccg gatcagcttg tcattctcgt cgtacttagt gttcatccgg gagtccagga
     7141 tctgtgccac gtgctttgtg atctgccggg tttccaccag ctgtctcttg atgaagccgg
     7201 ccttatccag ttcgctcagg ccgcctctct cggccttggt cagattgtcg aactttctct
     7261 gggtaatcag cttggcgttc agcagctgcc gccagtagtt cttcatcttc ttcacgacct
     7321 cttcggaggg cacgttgtcg ctcttgcccc ggttcttgtc gcttctggtc agcaccttgt
     7381 tgtcgatgga gtcgtccttc agaaagctct gaggcacgat atggtccaca tcgtagtcgg
     7441 acagccggtt gatgtccagt tcctggtcca cgtacatatc ccgcccattc tgcaggtagt
     7501 acaggtacag cttctcgttc tgcagctggg tgttttccac ggggtgttct ttcaggatct
     7561 ggctgcccag ctctttgatg ccctcttcga tccgcttcat tctctcgcgg ctgttcttct
     7621 gtcccttctg ggtggtctgg ttctctctgg ccatttcgat cacgatgttc tcgggcttgt
     7681 gccggcccat cactttcacg agctcgtcca ccaccttcac tgtctgcagg atgcccttct
     7741 taatggcggg gctgccggcc agattggcaa tgtgctcgtg caggctatcg ccctggccgg
     7801 acacctgggc tttctggatg tcctctttaa aggtcaggct gtcgtcgtgg atcagctgca
     7861 tgaagtttct gttggcgaag ccgtcggact tcaggaaatc caggattgtc ttgccggact
     7921 gcttgtcccg gatgccgttg atcagcttcc ggctcagcct gccccagccg gtgtatctcc
     7981 gccgcttcag ctgcttcatc actttgtcgt cgaacaggtg ggcataggtt ttcagccgtt
     8041 cctcgatcat ctctctgtcc tcaaacagtg tcagggtcag cacgatatct tccagaatgt
     8101 cctcgttttc ctcattgtcc aggaagtcct tgtccttgat aattttcagc agatcgtggt
     8161 atgtgcccag ggaggcgttg aaccgatctt ccacgccgga gatttccacg gagtcgaagc
     8221 actcgatttt cttgaagtag tcctctttca gctgcttcac ggtcactttc cggttggtct
     8281 tgaacagcag gtccacgatg gcctttttct gctcgccgct caggaaggcg ggctttctca
     8341 ttccctcggt cacgtatttc actttggtca gctcgttata cacggtgaag tactcgtaca
     8401 gcaggctgtg cttgggcagc accttctcgt tgggcaggtt cttatcgaag ttggtcatcc
     8461 gctcgatgaa gctctgggcg gaagcgccct tgtccaccac ttcctcgaag ttccaggggg
     8521 tgatggtttc ctcgctcttt ctggtcatcc aggcgaatct gctgtttccc ctggccagag
     8581 ggcccacgta gtaggggatg cggaaggtca ggatcttctc gatcttttcc cggttgtcct
     8641 tcaggaatgg gtaaaaatct tcctgccgcc gcagaatggc gtgcagctct cccaggtgga
     8701 tctggtgggg gatgctgccg ttgtcgaagg tccgctgctt ccgcagcagg tcctctctgt
     8761 tcagcttcac gagcagttcc tcggtgccgt ccatcttttc caggatgggc ttgatgaact
     8821 tgtagaactc ttcctggctg gctccgccgt caatgtagcc ggcgtagccg ttcttgctct
     8881 ggtcgaagaa aatctctttg tacttctcag gcagctgctg ccgcacgaga gctttcagca
     8941 gggtcaggtc ctggtggtgc tcgtcgtatc tcttgatcat agaggcgctc aggggggcct
     9001 tggtgatctc ggtgttcact ctcaggatgt cgctcagcag gatggcgtcg gacaggttct
     9061 tggcggccag aaacaggtcg gcgtactggt cgccgatctg ggccagcagg ttgtccaggt
     9121 cgtcgtcgta ggtgtccttg ctcagctgca gtttggcatc ctcggccagg tcgaagttgc
     9181 tcttgaagtt gggggtcagg cccaggctca gggcaatcag gtttccgaac aggccattct
     9241 tcttctcgcc gggcagctgg gcgatcagat tttccagccg tctgctcttg ctcagtctgg
     9301 cagacaggat ggccttggcg tccacgccgc tggcgttgat ggggttttcc tcgaacagct
     9361 ggttgtaggt ctgcaccagc tggatgaaca gcttgtccac gtcgctgttg tcggggttca
     9421 ggtcgccctc gatcaggaag tggccccgga acttgatcat gtgggccagg gccagataga
     9481 tcagccgcag gtcggccttg tcggtgctgt ccaccagttt ctttctcagg tggtagatgg
     9541 tggggtactt ctcgtggtag gccacctcgt ccacgatgtt gccgaagatg gggtgccgct
     9601 cgtgcttctt atcctcttcc accaggaagg actcttccag tctgtggaag aagctgtcgt
     9661 ccaccttggc catctcgttg ctgaagatct cttgcagata gcagatccgg ttcttccgtc
     9721 tggtgtatct tcttctggcg gttctcttca gccgggtggc ctcggctgtt tcgccgctgt
     9781 cgaacagcag ggctccgatc aggttcttct tgatgctgtg ccggtcggtg ttgcccagca
     9841 ccttgaattt cttgctgggc accttgtact cgtcggtgat cacggcccag cccacagagt
     9901 tggtgccgat ggccaggccg atgctgtact tcttgtcaga gccaccagaa gatccgccgc
     9961 tactctcagg tgtggcagac tcgcttgtgc caggtgtctc agatccgcta gagccgccgc
    10021 tagatcctcc gctcttaagt ctccaggtca cagaagggtg gatcaggcct gtagccagca
    10081 ggatgtgggg ggggatggtc tggtagtggc aattctgcag gtgcagtctg aagaaggcca
    10141 ggtggttctg ccatcttcta gagatcttca gacatggagg cagagacagg atgatgcagt
    10201 gcagttccag ggcgtacagc atcatccaca gaggagggta ctgaggccag tgggcctcat
    10261 cgccgggggg gtagttcacg aagttccgcc aacagtggta gtactcgctg gcccgcatga
    10321 tctggatagt cacgccgctg ttgaccagat ctctcaggcc ctgtctgttt ctctggtcca
    10381 tggcccagaa cagtctggcc acgtagatca ccagtgtcac tccggggtgt tggctcagga
    10441 actctctgat ggcctggctg cattcccagc aaggggacca gctcaggaac caggtgatgc
    10501 tgcagctgat gctgctgtgg aaccttctct cgctggtgaa cttcttgatg aaattcactt
    10561 ccacgtggtt ggtggtgttc ttgccagagc tccgccagat ctttctgctc atgccccact
    10621 tgatctcgta cagcaggcag gtttcctttc tcagttctct agggtcgtag aacacgtcga
    10681 actcccagct ctcgattctc cgccgcaggg tggggtcgcc tgtgctaggg cccttctcag
    10741 aggtcttgtc atcgtcatcc ttgtaatcga tatcatgatc tttataatca ccgtcatggt
    10801 ctttgtagtc catggtatat ctccttgttt cctgtgtgaa attgttatcc gctcacaatt
    10861 ccacacatta tacgagccga tgattaattg tcaacagctc atttcagaat atttgccaga
    10921 accgttatga tgtcggcgca aaaaacatta tccagaacgg gagtgcgcct tgagcgacac
    10981 gaattatgca gtgatttacg acctgcacag ccataccaca gcttccgatg gctgcctgac
    11041 gccagaagca ttggtgcacc gtgcagtcga taagcccgga tcagcttgca attcgcgcgc
    11101 gaaggcgaag cggcatttac gttgacacca tcgaatggtg caaaaccttt cgcggtatgg
    11161 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
    11221 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
    11281 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
    11341 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
    11401 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
    11461 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
    11521 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
    11581 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
    11641 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
    11701 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
    11761 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
    11821 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
    11881 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
    11941 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
    12001 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
    12061 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
    12121 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
    12181 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    12241 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa ccccgggctg
    12301 caggaattcc tcgagaccgg cgcgcccctg tttgacagct tatcatcgca gatccgggca
    12361 acgttgttgc cattggcggc cgcaacgagc tctagtgtac atcgggatcc tggcagttcc
    12421 ctactctcgc atggggagac cccacactac catcggcgct acggcgtttc acttctgagt
    12481 tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca
    12541 gaccgcttct gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa
    12601 aacagccaag cttatggctt cgtttcgcag cgaacttgga aaaaaaattt ttggcgattc
    12661 gccgctttcg tataacgaag taaaaaccta tctcagcgaa cacccaggcg gaaccgcaga
    12721 acgagcctgg aatcatattt gtgcgccttg aaatatccct ctccataccc cataaataaa
    12781 taatgacatt aatccttcat gaaggagggc ccttatggag aaaaaaatca ctggatatac
    12841 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
    12901 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
    12961 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
    13021 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
    13081 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
    13141 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
    13201 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
    13261 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
    13321 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
    13381 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
    13441 acagtactgc gatgagtggc agggcggggc gtaagctagc aggagacatg aacgatgaac
    13501 atcaaaaagt ttgcaaaaca agcaacagta ctaaccttta ctaccgcact gctggcagga
    13561 ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc catataagga aacatacggc
    13621 atttcccata ttacacgcca tgatatgctg caaatccctg aacagcaaaa aaatgaaaaa
    13681 tatcaagttc ctgaattcga ttcgtccaca attaaaaata tctcttctgc aaaaggcctg
    13741 gacgtttggg acagctggcc attacaaaac gctgacggca ctgtcgcaaa ctatcacggc
    13801 taccacatcg tctttgcatt agccggagat cctaaaaatg cggatgacac atcgatttac
    13861 atgttctatc aaaaagtcgg cgaaacttct attgacagct ggaaaaacgc tggccgcgtc
    13921 tttaaagaca gcgacaaatt cgatgcaaat gattctatcc taaaagacca aacacaagaa
    13981 tggtcaggtt cagccacatt tacatctgac ggaaaaatcc gtttattcta cactgatttc
    14041 tccggtaaac attacggcaa acaaacactg acaactgcac aagttaacgt atcagcatca
    14101 gacagctctt tgaacatcaa cggtgtagag gattataaat caatctttga cggtgacgga
    14161 aaaacgtatc aaaacgtaca gcagttcatc gatgaaggca actacagctc aggcgacaac
    14221 catacgctga gagatcctca ctacgtagaa gataaaggcc acaaatactt agtatttgaa
    14281 gcaaacactg gaactgaaga tggctaccaa ggcgaagaat ctttatttaa caaagcatac
    14341 tatggcaaaa gcacatcatt cttccgtcaa gaaagtcaaa aacttctgca aagcgataaa
    14401 aaacgcacgg ctgagttagc aaacggcgct ctcggtatga ttgagctaaa cgatgattac
    14461 acactgaaaa aagtgatgaa accgctgatc gcatctaaca cagtaacaga tgaaattgaa
    14521 cgcgcgaacg tctttaaaat gaacggcaaa tggtacctgt tcactgactc ccgcggatca
    14581 aaaatgacga ttgacggcat tacgtctaac gatatttaca tgcttggtta tgtttctaat
    14641 tctttaactg gcccatacaa gccgctgaac aaaactggcc ttgtgttaaa aatggatctt
    14701 gatcctaacg atgtaacctt tacttactca cacttcgctg tacctcaagc gaaaggaaac
    14761 aatgtcgtga ttacaagcta tatgacaaac agaggattct acgcagacaa acaatcaacg
    14821 tttgcgccaa gcttcctgct gaacatcaaa ggcaagaaaa catctgttgt caaagacagc
    14881 atccttgaac aaggacaatt aacagttaac aaataaacca ggtgctcggc gtggcaagac
    14941 tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc attggagaaa ttcttcaaat
    15001 tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc agcgaatgcc
    15061 gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg gctctggtaa
    15121 cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat gttccgcgtc
    15181 cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt taccctcagc
    15241 cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactcccg tactaactgt
    15301 cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat
    15361 aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa
    15421 aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc gcccttgtgg
    15481 ggcgctgctg cccttgccca atatgcccgg ccagaggccg gatagctggt ctattcgctg
    15541 cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc aaagcccgct
    15601 aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct ttagcggcct
    15661 ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat
    15721 cattcagccc ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc
    15781 gcgttcaagg tacgcatcca ttgccgccat gagccgatcc tccggccact cgctgctgtt
    15841 caccttggcc aaaatcatgg cccccaccag caccttgcgc cttgtttcgt tcttgcgctc
    15901 ttgctgctgt tcccttgccc gcacccgctg aatttcggca ttgattcgcg ctcgttgttc
    15961 ttcgagcttg gccagccgat ccgccgcctt gttgctcccc ttaaccatct tgacacccca
    16021 ttgttaatgt gctgtctcgt aggctatcat ggaggcacag cggcggcaat cccgacccta
    16081 ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga aactggccta acggccaccc
    16141 ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa gcaacagcga
    16201 ggcagcatgg cgatttatca ccttacggcg aaaaccggca gcaggtcggg cggccaatcg
    16261 gccagggcca aggccgacta catccagcgc gaaggcaagt atgcccgcga catggatgaa
    16321 gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc cgactactgg
    16381 gatgctgccg acctgtatga acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc
    16441 ctgccggtcg agctgaccct cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac
    16501 ctgaccggtg ccgagcgcct gccg
//
