LOCUS       Exported               14899 bp ds-DNA     circular SYN 20-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 20, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pLOT_File_Version 2.1
            Plasmid Name: pAAVS1-Blast-PECAM-Neo
            Project Codon Table = Standard
            Note: Source vector: pAAVS1-(Puro)-MCS-Neo digested with NotI + SpeI
            and
            Note: ligated to PECAM promoter that had been ligated to 
            pAAVS-Neo-MCS-mScarlet.
            ENZYME = BamHI
            ENZYME = NcoI
            ENZYME = PvuII
            RE_Filter:	1	1	1	15087
FEATURES             Location/Qualifiers
     source          1..14899
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..589
                     /gene="pBR322 Ori"
                     /label=pBR322 Ori
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632256"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    1..29
                     /gene="RNAII Promoter"
                     /label=RNAII Promoter
                     /label=nonstandard type:  misc
                     /note="pUC Rep Ori RNAII Promoter"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454016"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    37..589
                     /gene="pBR322 Ori RNAII transcript"
                     /label=pBR322 Ori RNAII transcript
                     /note="pBR322 Ori RNAII transcript"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    complement(40..156)
                     /gene="pBR322 Ori RNAI transcript"
                     /label=pBR322 Ori RNAI transcript
                     /note="pBR322 Ori RNAI transcript"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    43..75
                     /gene="Loop 1"
                     /label=Loop 1
                     /note="pUC Rep Ori Loop 1.  Initiates RNAI/RNAII 
                     interaction."
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=255"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    43..68
                     /gene="RNAI Terminator"
                     /label=RNAI Terminator
                     /label=nonstandard type:  misc
                     /note="pUC Rep Ori RNAI transcription terminator"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    78..106
                     /gene="Loop 2"
                     /label=Loop 2
                     /note="pUC Rep Ori Loop 2.  Initiates RNAI/RNAII 
                     interaction."
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=255"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    109..138
                     /gene="Loop 3"
                     /label=Loop 3
                     /note="pUC Rep Ori Loop 3.  Initiates RNAI/RNAII 
                     interaction."
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=255"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     promoter        complement(154..183)
                     /gene="RNAI Promoter"
                     /label=RNAI Promoter
                     /note="pUC Rep Ori RNAI Promoter"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     promoter        913..942
                     /gene="LacZ Promoter"
                     /label=LacZ Promoter
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     protein_bind    947..969
                     /gene="LacO"
                     /label=LacO
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=10280598"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    1058..1846
                     /gene="AAVS1 5' Homology Arm"
                     /label=AAVS1 5' Homology Arm
                     /note="Validated homology arm for targeted integration into
                     the AAVS1 safe harbor locus"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=16744576"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    1857..1877
                     /gene="Splice Acceptor"
                     /label=Splice Acceptor
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=65280"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_signal     1899..1955
                     /gene="T2A-Like Spacer Peptide"
                     /label=T2A-Like Spacer Peptide
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632256"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    1971..2381
                     /gene="BlastR"
                     /label=BlastR
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=33023"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_codon_table=Standard"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    2403..2626
                     /gene="BGH polyA"
                     /label=BGH polyA
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8421631"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    2711..10847
                     /gene="PECAM Promoter"
                     /label=PECAM Promoter
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454016"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    2776..2795
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    3682..3701
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    complement(3882..3901)
                     /gene="Rev"
                     /label=Rev
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    4602..4622
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    complement(4833..4852)
                     /gene="Rev"
                     /label=Rev
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    5387..5406
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    6171..6189
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    complement(6864..6883)
                     /gene="Rev"
                     /label=Rev
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    7090..7109
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    complement(7352..7371)
                     /gene="Rev"
                     /label=Rev
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    8292..8311
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    complement(8554..8573)
                     /gene="Rev"
                     /label=Rev
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    9674..9693
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    complement(9922..9941)
                     /gene="Rev"
                     /label=Rev
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    10312..10331
                     /gene="Fwd"
                     /label=Fwd
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     misc_feature    10863..11657
                     /gene="Neo/Kan"
                     /label=Neo/Kan
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=65535"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     primer_bind     complement(11405..11419)
                     /gene="Kan_dNco_R"
                     /label=Kan_dNco_R
                     /note="Kan_dNco_R (8695) "
                     /note="[Category: BglBricks]"
                     /note="Location: JM 09:72 (JM 09:72)"
                     /note="Removes the NcoI site from KanR in pBgl-NU and pCR4,
                     t2235C_F."
                     /note="Sequence: gcatcgccGtgggtcacgacgagatcctcg"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=13124425"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=7"
                     /note="/pLOT_fWidth=3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=32768"
     primer_bind     11433..11447
                     /gene="Kan_dNco_F"
                     /label=Kan_dNco_F
                     /note="Kan_dNco_F (10559) >>>>"
                     /note="[Category: BglBricks]"
                     /note="Location: JM 09:71 (JM 09:71)"
                     /note="Removes the NcoI site from KanR in pBgl-NU and pCR4,
                     t2235C_F."
                     /note="Sequence: gtgacccaCggcgatgcctgcttgccgaat"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=4463400"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=7"
                     /note="/pLOT_fWidth=3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=32768"
     primer_bind     complement(11640..11654)
                     /gene="Pme_Neo_R"
                     /label=Pme_Neo_R
                     /note="Pme_Neo_R (10766) "
                     /note="[Category: Burridge Lab Project]"
                     /note="Location: JM 13:15 (JM 13:15)"
                     /note="Amplifies Neo from (dBN) with 1/2 PmeI site to 
                     replace the sequence with the NcoI site in it in the pAAVS1
                     plasmids."
                     /note="Sequence: aaacTCAGAAGAACTCGTCAAG"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=1637908"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=7"
                     /note="/pLOT_fWidth=3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=32768"
     misc_feature    11658..11670
                     /gene="MCS"
                     /label=MCS
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=16744448"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    complement(11665..11695)
                     /gene="3' HA"
                     /label=3' HA
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=1"
                     /note="/pLOT_rOffset=1.1"
                     /note="/pLOT_tcolor=0"
     polyA_signal    11680..12183
                     /gene="rb_glob_pa_terminator"
                     /label=rb_glob_pa_terminator
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8421631"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    12263..13082
                     /gene="AAVS1 3' Homology Arm"
                     /label=AAVS1 3' Homology Arm
                     /note="Validated homology arm for targeted integration into
                     the AAVS1 safe harbor locus"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=16744576"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     promoter        complement(13099..13118)
                     /gene="T7"
                     /label=T7
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12816842"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     primer_bind     complement(13099..13113)
                     /gene="T7"
                     /label=T7
                     /note="T7 (12230) "
                     /note="[Category: Eurofins Standard Primers]"
                     /note="Location: Eurofins"
                     /note="Eurofins Standard Primers"
                     /note="Sequence: TAATACGACTCACTATAGGG"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=632607"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=7"
                     /note="/pLOT_fWidth=3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=32768"
     CDS             13213..13281
                     /codon_start=1
                     /gene="LacZ alpha"
                     /label=LacZ alpha
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=16744576"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     rep_origin      13283..13728
                     /gene="f1 Origin of Replication"
                     /label=f1 Origin of Replication
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632256"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     promoter        13799..13827
                     /gene="AmpR Promoter"
                     /label=AmpR Promoter
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     misc_feature    13869..14069
                     /gene="B-lactamase Secretion Signal"
                     /label=B-lactamase Secretion Signal
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
     CDS             14067..14726
                     /codon_start=1
                     /gene="Amp ORF"
                     /label=Amp ORF
                     /note="Confers resistance to ampicillin"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=65535"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tgtgctttct ctgaccagca ttctctcccc tgggcctgtg
     1081 ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca gatccttccc
     1141 tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc tctgctgtgt
     1201 tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc acgtgatgtc
     1261 ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc cctcacccaa
     1321 ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg cctgtgccat
     1381 ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc gcctcccctt
     1441 cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc gtctccctgg
     1501 ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc tcctgtggat
     1561 tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta cctctctagt
     1621 ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag agctcagcta
     1681 gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt tgctgcctcc
     1741 agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt taatgtggct
     1801 ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc ttctgacctc
     1861 ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga ggaagtcttc
     1921 taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagggtacc atggccaagc
     1981 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     2041 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     2101 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     2161 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     2221 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     2281 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     2341 tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtctagaggg cccgtttaaa
     2401 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     2461 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     2521 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     2581 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     2641 tggactagcg gtggcggcct cgacattgat tattgactag tggcgcgccg gatccttaat
     2701 taagtctaga gtcgagctct ctatgctaat gaggtaccta gaggcccaga gggcttagcc
     2761 actcagcttc ccttcccaga cgttcctccc tgtaaaaggt ccaccctgag aactgggcat
     2821 ggtatattat gtatctaatt tttttgccat gaaggaacaa taaactgttt agaaccacag
     2881 actgtctctt ccctcaagat ccaactcggg gaaccacaga gaagacattt gactatgaac
     2941 gcacagctta attctaccac agaaggcctc tccgtgcttc tagccacaac tgccaccaag
     3001 cccgcctcca aacaccgctg ggataagcca gtgctcccgc ccccacttcc accccaggct
     3061 agacactgtc cccagcccga gggcccctga ctcagttctc agttcctcct tcattcccct
     3121 cagcgcctgg gtgtctaaga gtcagaagac cccctcagcc cctgcctgct ccagggatgg
     3181 aaccagctcc gactttccca cattcccaca tttaagactg tgctttccca ccccagagcg
     3241 atgtctctga gcacccaccc tagcactgga atgggataag ggcagtcaaa ctctccacat
     3301 ctgcctcccc aagaggttgt gaccagtggc ggggaggaca cagacccact ttagaggtgt
     3361 cgcctgctgg ggaagacttg gaaccaattt tattttattg tattttttag gcagagtttg
     3421 atgtagccta gcacttcctc taactcatta tacataactt atatactgaa cttctggtct
     3481 tcctgcctcc acctcctaag ccagtggttc tcatccttcc taattctgcg actttgacac
     3541 agttcttcat gctgtggtaa ccctccacca ccaccataaa attactttcc ttattattta
     3601 ataactgtaa ttttgctact gttatgaatc ttaatgtaaa tatccgatat gcaaggatat
     3661 ctgatatgcg acctccgaag gggtctttct tgaccccaca agttgagaac cggtgtccta
     3721 aatgagggga tcgaggcgtg catcactagg ccaggtttac agagacacag atgaaggctt
     3781 acactcttac acataaaata aataaaccct aaaaaaacaa caacaactag agatgatttt
     3841 atttttagat aatttgaagc tgagcgtagt gactgactca cacctgtaat cccacatggg
     3901 aaactgaggc aggaggctcc cagtgagcat agagcatacg tgtacacata agaaaaccca
     3961 aagaggaagt tcctctggcc tgtttacaca agagatagca tgctacacac cacacatagg
     4021 gtgactgaca ccttcaagcc tagcagttga ccaggggaaa cccaaaatgc atgtggctgt
     4081 gagaaattca aggaccagag atggtggagg atatcaggta ttaggtgcca ggtactgcta
     4141 ggcaccatta agaggtgagg cctcgctgga gtagactgcg gggtcccaag gctacgtaca
     4201 ctgcctagac atgttagccc agagcaggga ggaagcagtc ctgctcctgg tggcctcagg
     4261 gatgagattt ttgggttttg aatgaatttt gatctcactc ttactccaga agagttcagc
     4321 aggttgcatg gctttcaggg ccccacagtg ggtgagggtt tgtttaaaaa ggtgtgtcat
     4381 tggggggggg ggggctttga ggtctccaat gctcaagcca ggcccagtgg ttcagtctca
     4441 cttcttgctg ccagtggatc cagatgtaga actctcaggt acctgtccag caccatgtct
     4501 gcctgtgtgc agccatgctc cctgccatga tgataatgga ctagacttct gaactctaat
     4561 aagccagccc caatcgaatg tttcccttca tacaagttgc cgtggtcacg gtgtctcatt
     4621 gcagtgataa actctaacaa gacaggtaca gagaagttgt gccagctaaa catagagttc
     4681 catggcttag ataaggctct ctaaaagtcc tgttcttacc tggtaagagg aaactatcca
     4741 taggccgtga ctaccatact gtgaactcaa gggcttgagc tacaaagtaa aaccctgcct
     4801 ggccggggac agagttgata gagtacttcc ttaacatccg caatgaaact cctgggtaca
     4861 atccccagca ctacatttaa aacgaaaagg gaccgtgtga gggctcacca ggcaaaggca
     4921 tctgccacta agcccgagga cctgagttca atccctggaa gccagtggtg aaaggagagg
     4981 tcccacatca tcaagttatc ccctgacctt catacgtgtg gaatagctgt cctcctccaa
     5041 atgaattttt aaaagaaagt aaagaagaaa ctaacaccct gtctacacaa acaaacacac
     5101 acacacacat acacacacac acacacacac acacacacaa acagtttaac atagtggtaa
     5161 acaaatgaac agagcccccc cctcaaacaa acaaaaccca acaatcacaa aattgtctta
     5221 gagaggagaa gagggcatgt ttctatctgc ttgccgcatg tgagccctgg gttccactcc
     5281 agcaccagga aaagagatag tgaaaataaa acctgagagc tgggattccc agcgctagac
     5341 aagagctagg ccagtgagca cagctgactc attgcgagac tccgaatcct cagaagtcac
     5401 caacggactt tggttgtctc ctgcttggat ccaaaagttc tctaatttca ctgagttact
     5461 ggtgaggtta agggaggaag actgagagtt caaggtcagc agtgggctac caagtctgag
     5521 gctagcctgg cctatatact gagaccctgc ctcaaaaaac aaaacaagcc ggcccggtgt
     5581 tggcacacgc ctttaatctc aacgcttggg aggcagaggc aagcagattt ctgagttcga
     5641 ggccagcctg gtcttcaaag tgagttccag gacagccagg gctatacaga gaaaccctgt
     5701 cttggaaaac aacaacaaca acaacaaaag aaaaaagaaa acaaaacaaa aacaccacca
     5761 ccaacaacaa cacataaagc caaggccagg gctcagtggg agaagtcgaa ggtcagattg
     5821 gctatataat gagcccccct ctcggggtgg ggttgggttt tgttgttgtg taaatagagc
     5881 atctaactgg aggttccaca tgtaccaact tctcaaagca gagatggctg ttcatgccct
     5941 gtttgcttct ttgtgaaggt ttcatgggtt tatgtcctcc aagaggttat atgaatataa
     6001 acagggaaat gatgaaatta tgctatacta ttcactgtgg attttcatac acacataaaa
     6061 tagagaaatt cagaaacgac ccagtgagac attctcaaga tttgacctct taccaggaaa
     6121 tgagcggcca caggccagct ctgctgtggg aagggccgtt agttactcaa cccacagttg
     6181 tcagcagtgg gacccactca gcccagactt gcatcctgtc tgtcgtgagg gatgaccccg
     6241 agccctttat cctcctgggt cccgggcagg caccaccaca cttggcttat gagatgctga
     6301 ggagtgaacc tacaactctg agtgtagcat acttggcaag catcacacac cctccgggag
     6361 acacagcatc gttcttagca ccaggaaaac gcctgggaaa tggtacctgg ggtctatctg
     6421 tcacagaata aatgggagtt tcaaagcttc atctcttctc tgtttctaga aatttattaa
     6481 atgcagaacg atttgtaaca acatagtctc caaactgggg atgtggatca gcagtagacc
     6541 acccacctag catgcataaa gccctcggct cggtcctcag gactgccaga aataaagaaa
     6601 ttggtaagtt ctttaggctc ccagacttgc tcatactgtg tggtctccac cttagttatg
     6661 ggctactgta cagacagcat cctgaacagt ctcctttagc tagatagggt tgagagttgt
     6721 tttattatta ttgtttgttt gtttgtttgt tcgtttgttt gtttttctga gacagggttt
     6781 ctctgtgtag cgctggctgt cctggaactc actctgtaga ccaggctggc ctcgaactca
     6841 gaaatccacc tgcctctgcc tcccaagtgc tgggattaaa ggtgtgcacc actaagcctg
     6901 gctatttgga agtagttgtt ttttttattt ttaattcctg tagtcttttt gcttctattt
     6961 gttgaaacag gaatgctagc acatctgcat ttcttggtaa ttatttttct cttgatgcta
     7021 ggaaattgac gccagtgccc ttcaaatgct aggcaagcac ttggccactg agccatacct
     7081 tacccagccc cactccccta cctccgtatt ttttggtgac tgaggcaagg tgcccgtggt
     7141 aataacaaac cctgggcctc atggataatc acttctctcc ttcacattgc aggacaacta
     7201 taggaaacac tacaggggcc tctccaggcc aggcactgtc cacagtgctg agtgggtcaa
     7261 caacacgccc tccgcccccc ccccccactt cataatagat gcaaaagaat taagatcaaa
     7321 caaaacatta catactgtta ctgttcatac tattgttcct tccgttgttc gtttttatca
     7381 tgtgcatgtg tgtattctgc atatgggttt atgtacatta acgcaatgtc caaggagact
     7441 agaaaggggt gtccattccc tgaagaggag ctagagttat gggcagttgt agctgcccaa
     7501 catggctgct gggcacccct cctctgcaaa agtagtaaga actcttaact cttgtgccat
     7561 ccctatcttc ccttccctac tgtcaatcca accagcccag ttcatcagtt ggaatcccag
     7621 gaatgcttcc ccaaagggaa ttcggttcca cctaaataaa gacatgctag aagggacttg
     7681 tctttctttg aaaggaagtc tccggcctcc aggcagctgt gagcagctat ctgaagcatc
     7741 ccaccctaca actccggcct tgtcatttcc ctccttttct atttcctctt ccccctgata
     7801 gtgatcaaag tctggaaggg gataccctga gcatcagcct ccgaggcccc catgctctct
     7861 gcctcgctgc tgcccgctac cccccattgc aaaaggcatg gcttgctctc tccttcattc
     7921 ccctgaaatt ttataatttg gctgttctgt catttttctc aaactcgaac tgtcctctag
     7981 gtttaaccaa aaacaaaaaa acctcaatta aagcaccaag tgtggctttt tgttgttttt
     8041 gagacagcaa ctcactatct tggctggctt ggaactggta aggtagatca ggctggcttc
     8101 gaatttacag aaatccacct gtttctgcct cttgagtgct gggattaaga gatgtgccac
     8161 cacacttggc atgtgtggat ttttttttaa gatttattta ttttatgtat atgagtacac
     8221 tgtagctgtc ttcagacata caagaagagg gcatcagatc ccattacaga tggttgtgag
     8281 ccaccatgcg gttgctgcga attgaactca ggacctctgg aagagcaggc agtgctctta
     8341 atctctgagc catctctcca gccaatgtgt ggcttttgta aggagggtgg tattgacacg
     8401 gctgatttct ttgacacaca tccacttgtg taacacgcac ctggatcact gggaggaacc
     8461 tcatgtcgcc actcagccag caggagtatc acaggacgga agagggggaa accttgtaga
     8521 aaactcaaga ggccaggtgc tgtggcacac acctctaatt ctagcgctcc ggaagcagag
     8581 aagaaagaac tgccaagagt ttgaggccac tctgggtcta caatgcaaat tccaggccag
     8641 gcaggattac acagtgaaac cctgcctcaa ggggagatgg gggtgcgggg agagaaatgg
     8701 ctcggtgatt aagagcactt gttcttgcag aactaggatt tcatgcactg acatggtggc
     8761 agctcacgac catctaatac acatgaagaa taaataaata aaaataaaaa tagggccgtt
     8821 gagacagctt agcaagcaaa ggtacttact gctgtgcctg agtttgtgga atcccaggac
     8881 ccacagggta gaagagaatt aactcatgca agttatcctc tgacctctaa aagcacctca
     8941 caggctgcac acaagctcag gcacacacac taaataaaca tgcattcaaa attttaaata
     9001 aagaaaattt cccaaatgag ccaggccaag ccctaacgga gaacataacc agcttcgata
     9061 acgtggcagc cgctctgccc tagacagcct ccaaaacagc cctcggccct gttacatctc
     9121 ccacatgacc ataccaaggc tatcacagga ctgactcccc caatggagcc aggacactcc
     9181 tcctgatgtt agtgtacagc ttccatctag tcaagctctc tcctccagga cctcagccct
     9241 gggtcaaaag tcacatcctg caagagcagc taagaaggcg gtgtctcaca ggatgatgaa
     9301 cggaggcctt cagtgtagcc acaaaagtca gctcaaaagc agacacccag gcttagtcag
     9361 ccttcgtgga acaactttga ccttgaccaa aagggtctat catcacaaga ttgtgagcca
     9421 gagttaccca caagactggg tgaggtgctg agtttatact acttccagcc agagtcttgg
     9481 aataattcaa gattttaaaa aagatatttg tttattttaa gataatcttg ctatgtagct
     9541 caggctagcc ttaaactctt cacaatcctg ccttggtttc ccaagtacgg ggactacagt
     9601 gtgagctggc acacccctct cccagcccct ttcattttct gttaacacaa agatactgag
     9661 tttggctggg cactccccac tctgtaacct tggcagccct agaaagtcca acctcccagg
     9721 ttcggctctc tgaggaagca ctgcactgag tgtttgtttg tttgtttgtt tgtttgtttg
     9781 cgacagtgtt tctctgtgct gtcctggaat agttctatag agcaaactgg ccatgaaccc
     9841 actatgtagc caaggattag gtaggatttt gaactttatg gttttcctgt ctctatctcc
     9901 caagtcctag tgttataggc ttgtaccacc atgtgtggct ttagggatca aaaccaggga
     9961 gccacacatg ccagatgaac tctgaggcat atccccagtc ctgttctttg gtcttcgatt
    10021 ttgcaaaatc aagcaaacag gaaaataata cagccagcag atcttgagac ttctccacgt
    10081 ctataaaaca gaattcttac tccttgctat aagaaaataa tataataata atacagccag
    10141 cagatcttga gacttctcca tgtctataaa acagaattct tactccttgc tataagaaca
    10201 tgccctccga ggagaagcac actgaaactg cacagaggtc tcaccaggat acaggactat
    10261 tcacggcaag ggggggccca aggctctagg tgtgcacagt attggaaatg tcccacagac
    10321 ctcctgtcaa tggacaatgc ccccaccccc accccatgct cctcatcaag cctcaaactg
    10381 ttcaaaaaag tcaatggcca acagggcaca gaacagccta aagagatcta agagaggagg
    10441 gtctacaaac tggagttcca ggacatccag ggcttcacag agaaaccctg tctggggggt
    10501 tggggggggg gaggaggagg ggtacaaaca atgttaaata ctaacggttt aatgaggggg
    10561 cttccttccc aaattaaggt cctcttagta tgatgagcca cccccagcca cctggctctc
    10621 aacctggaaa tgactcagag gggaagagga actacctacc attgtcagga aaattacaga
    10681 gctcttacgc gtgctagaca aagccattgg gttcagtggt ccctccggaa ggccagtcat
    10741 ttcctgagga gatatcaggc cagcccagac ctcattgttc ttggtttcca gctatggccg
    10801 ccattacctg acaagtacca gagctggtct ctgcgcggta ggttaccgac gtcgcggccg
    10861 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
    10921 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
    10981 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
    11041 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
    11101 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
    11161 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
    11221 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
    11281 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
    11341 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
    11401 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
    11461 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
    11521 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
    11581 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    11641 ttgacgagtt cttctgacct gcaggaattc actcctcagg tgcaggctgc ctatcagaag
    11701 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
    11761 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
    11821 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
    11881 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
    11941 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
    12001 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
    12061 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
    12121 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
    12181 ctcttatgaa gatccctcga cctgcagccc aagcttggat ccctcgagtt aattaaatct
    12241 agaagtcgac agtactaagc tttgacagaa aagccccatc cttaggcctc ctccttccta
    12301 gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta tctggtgaca
    12361 cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt tgcttacgat
    12421 ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga agggggggat
    12481 gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga acctgagctg
    12541 ctctgacgcg gctgtctggt gcgtttcact gatcctggtg ctgcagcttc cttacacttc
    12601 ccaagaggag aagcagtttg gaaaaacaaa atcagaataa gttggtcctg agttctaact
    12661 ttggctcttc acctttctag tccccaattt atattgttcc tccgtgcgtc agttttacct
    12721 gtgagataag gccagtagcc agccccgtcc tggcagggct gtggtgagga ggggggtgtc
    12781 cgtgtggaaa actccctttg tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg
    12841 cctctactcc ctttctcttt ctccatcctt ctttccttaa agagtcccca gtgctatctg
    12901 ggacatattc ctccgcccag agcagggtcc cgcttcccta aggccctgct ctgggcttct
    12961 gggtttgagt ccttggcaag cccaggagag gcgctcaggc ttccctgtcc cccttcctcg
    13021 tccaccatct catgcccctg gctctcctgc cccttcccta caggggttcc tggctctgct
    13081 ctagcgatcg ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
    13141 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    13201 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    13261 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    13321 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    13381 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    13441 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    13501 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    13561 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    13621 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    13681 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
    13741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    13801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    13861 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    13921 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    13981 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    14041 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    14101 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    14161 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    14221 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    14281 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    14341 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    14401 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    14461 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    14521 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    14581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    14641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    14701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    14761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    14821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    14881 aaaagatcaa aggatcttc
//
