LOCUS       pBBR15.2-2H12.D11opt-scarRef        6270 bp ds-DNA     circular SYN 14-MAY-2024
DEFINITION  Broad-host-range plasmid with cdGreen2.1 with a mScarlet-I operon
            fusion as reference FP. UJ11241.
ACCESSION
VERSION     .
KEYWORDS    pAK206.
SOURCE      
  ORGANISM  .
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 15, 2017 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
COMMENT     Vector molecule: Fragment 2 Fragment ends: BglII and SpeI Fragment
            size: 3747 Insert molecule: Fragment 2 Fragment ends: SpeI and
            BamHI Fragment size: 712.
FEATURES             Location/Qualifiers
     source          complement(1..6270)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     -35             303..308
                     /created_by="klotza"
                     /modified_by="kaczmarc"
                     /label="-35_1"
     promoter        303..331
                     /created_by="kaczmarc"
                     /label="Prom_hybrid"
     misc_feature    307..2426
                     /Original_Bases="CACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAG
                     CGGATAACAATTTCACACAGGAAACAGCTATGACATGATTACGAATTCGAGCTCGGTA
                     CCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCACTGGCCGTCGTTT
                     TACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACA
                     TCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAA
                     CAGTTGCGCAGCCTGAATGGCGAATGGCGATAAGCT"
                     /label="CACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACA
                     ATTTCACACAGGAAACAGCTATGACATGATTACGAATTCGAGCTCGGTACCCGGGGAT
                     CCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCACTGGCCGTCGTTTTACAACGTC
                     GTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTT
                     CGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC
                     AGCCTGAATGGCGAATGGCGATAAGCT"
                     /note="Geneious type: Editing History Replacement"
     -35             315..320
                     /created_by="kaczmarc"
                     /modified_by="kaczmarc"
                     /label="-35_2"
     promoter        315..343
                     /created_by="kaczmarc"
                     /label="Ptet_const"
     -10             326..331
                     /created_by="kaczmarc"
                     /label="-10_1"
     -10             338..343
                     /created_by="kaczmarc"
                     /modified_by="kaczmarc"
                     /label="-10_2"
     RBS             390..398
                     /created_by="kaczmarc"
     CDS             405..1700
                     /created_by="kaczmarc"
                     /modified_by="kaczmarc"
                     /label="2H12.D11opt (cdGreen2.1)"
     RBS             1689..1696
                     /created_by="kaczmarc"
                     /label="RBS-like"
     CDS             1707..2408
                     /created_by="kaczmarc"
                     /label="mScarlet-I"
     source          <1707..>2413
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /db_xref="taxon:511145"
     source          complement(<2427..>2431)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2432..2464
                     /created_by="kaczmarc"
                     /label="overhang"
     primer_bind     complement(2767..2786)
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="12759996912493003a04862e-55ca-486b-9de2
                     -a8a4c87a005b"
                     /Sequence="AACAAGCCATGAAAACCGCC"
                     /created_by="primer3"
                     /label="11458_pUC18T-Gm seq fw"
     CDS             2880..3413
                     /note="aacC1"
                     /note="gentamycin acetyl transferase"
                     /modified_by="kaczmarc"
     misc_feature    3461
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(3816..4478)
                     /note="REP"
ORIGIN      
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgacgtct aagaacatgt tgcagctggc acgacaggtt tcccgactgg
      241 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      301 gctttatagc tagcttgaca tcccatagag tgatagagat actgagcaca tcagcatatg
      361 aaagtgggtc ttaataataa taattccgaa ggaggaggtg atccatgaat tcggaaccgc
      421 cgccgaagct ggtcctggac ctggaacgcc tggcgcacgt cccgcaggaa aaggcgggcc
      481 cgctgcagcg ctatgccgcc accatccagt cgcagcgggg cgactacaac ggcaaggtcc
      541 tgtcgatccg gccggcggac ctgcgcaccc tggccgtcat ctatgaccag tcgccgtcgg
      601 tcctgaccga gcagctgatc tcgtggggcg tcctggatgc cgatgcgcgc cgcgccgtcg
      661 cgcatgaaga gaacccgaag aacatgccgg tgccgtacaa cgtgttcatc atggccgaca
      721 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gatggcggcg
      781 tccagctggc ctaccactac cagcagaaca ccccgatcgg cgacggcccg gtcctgctgc
      841 cggacaacca ttacctgggc gtccagtcga agctgtcgaa ggacccgaac gagaagcgcg
      901 accacatggt gctgctggag ttcgtcaccg ccgcgggcat caccctgggc atggacgagc
      961 tgtacaaggg cggcaccggc ggctcgggcg tcagcaaggg cgaagaactg ttcaccggcg
     1021 tcgtcccgat cctggtcgag ctggacggcg acgtcaacgg ccacaagttc tcggtcagcg
     1081 gcgaaggcga gggcgacgcc acctatggca agctgaccct gaagttcatc tgcaccaccg
     1141 gcaagctgcc cgtgccgtgg ccgacgctgg tcaccacgct gacctatggc gtgcagtgct
     1201 tctcgcgcta cccggaccac atgaagcagc acgatttctt caagagcgcc atgccggaag
     1261 gctacatcca ggaacggacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1321 aggtcaagtt cgaaggcgac accctggtca accgcatcga gctgaagggc atcgacttca
     1381 aggaagatgg caacatcctg ggccacaagc tggaatacaa catgctgtcg gagcccgaac
     1441 cgccgccgaa gctggtgctg gatctggaac ggctggccca tgtgccgcag gaaaaggcgg
     1501 gcccgctgca gcggtatgcg gcgacgatcc agagccagcg cggcgattat aacggcaagg
     1561 tgctgagcat ccggccggcg gatctgcgga cgctggcggt gatctacgat cagagcccga
     1621 gcgtgctgac ggaacagctg atctcctggg gcgtgctgga cgcggatgcc cggcgcgcgg
     1681 tcgcccacga ggaaaactaa actagtatga gcaagaaata tggtgaagcc gtgatcaaag
     1741 aatttatgcg ctttaaagtt cacatggaag gtagcatgaa tggccacgaa tttgaaattg
     1801 aaggtgaagg cgaaggtcgt ccgtatgaag gcacccagac cgcaaaactg aaagttacca
     1861 aaggtggtcc gctgccgttt agctgggata ttctgagtcc gcagtttatg tatggtagcc
     1921 gtgcatttat caaacatccg gcagatattc cggattacta taaacagagc tttccggaag
     1981 gttttaaatg ggaacgtgtg atgaattttg aagatggtgg tgcagttacc gttacacagg
     2041 ataccagcct ggaagatggc accctgatct ataaagttaa actgcgtggc accaattttc
     2101 cgcctgatgg tccggttatg cagaaaaaaa ccatgggttg ggaagcaagc accgaacgtc
     2161 tgtatcctga agatggcgtt ctgaaaggtg atatcaaaat ggcactgcgt ctgaaagatg
     2221 gcggtcgtta tctggcagat ttcaaaacca cctacaaagc caaaaaaccg gttcagatgc
     2281 ctggtgcata taatgttgat cgcaaactgg atattaccag ccacaatgaa gattataccg
     2341 tggtggaaca gtatgaacgt agcgaaggcc gtcatagtac cggtggtatg gatgaactgt
     2401 acaagtgagc tagtaaaaaa ggatccagct tcacgctgcc gcaagcactc agggcgcaag
     2461 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     2521 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     2581 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     2641 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     2701 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc ctggtaacgg
     2761 cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc
     2821 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2881 tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg
     2941 gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc
     3001 gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc
     3061 agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg
     3121 ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc
     3181 agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac cggaggcagg
     3241 gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg
     3301 tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg
     3361 gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg
     3421 ttcaagccga gatcggcttc ccggccgcgg agttgttact tgttcggttc agggcagggt
     3481 cgttaaatag ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt
     3541 gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta
     3601 ataattgacg atatgatcat ttattctgcc tcccagagcc tgataaaaac ggtgaatccg
     3661 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
     3721 gcaacgcggg gaggcagaca aggtataggg cggcgaggcg gctacagccg atagtctgga
     3781 acagcgcact tacgggttgc tgcgcaaccc aagtgctacc ggcgcggcag cgtgacccgt
     3841 gtcggcggct ccaacggctc gccatcgtcc agaaaacacg gctcatcggg catcggcagg
     3901 cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca aggctggcag gtctggttcc
     3961 atgcccggaa tgccgggctg gctgggcggc tcctcgccgg ggccggtcgg tagttgctgc
     4021 tcgcccggat acagggtcgg gatgcggcgc aggtcgccat gccccaacag cgattcgtcc
     4081 tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg acaggcgcaa ctggtcgcgg
     4141 ggctggcccc acgccacgcg gtcattgacc acgtaggccg acacggtgcc ggggccgttg
     4201 agcttcacga cggagatcca gcgctcggcc accaagtcct tgactgcgta ttggaccgtc
     4261 cgcaaagaac gtccgatgag cttggaaagt gtcttctggc tgaccaccac ggcgttctgg
     4321 tggcccatct gcgccacgag gtgatgcagc agcattgccg ccgtgggttt cctcgcaata
     4381 agcccggccc acgcctcatg cgctttgcgt tccgtttgca cccagtgacc gggcttgttc
     4441 ttggcttgaa tgccgatttc tctggactgc gtggccatgc ttatctccat gcggtagggt
     4501 gccgcacggt tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat
     4561 tatgcgttgc gtaaaagtgg cagtcaatta cagattttct ttaacctacg caatgagcta
     4621 ttgcgggggg tgccgcaatg agctgttgcg tacccccctt ttttaagttg ttgattttta
     4681 agtctttcgc atttcgccct atatctagtt ctttggtgcc caaagaaggg cacccctgcg
     4741 gggttccccc acgccttcgg cgcggctccc cctccggcaa aaagtggccc ctccggggct
     4801 tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc ttggaacccc
     4861 cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg cttcgccttc gactgccccc
     4921 actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc
     4981 gcgagccttg acccgccttc cacttggtgt ccaaccggca agcgaagcgc gcaggccgca
     5041 ggccggaggc ttttccccag agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc
     5101 gcagttggag ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg
     5161 tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt gtccacgggc
     5221 cgagcgaagc gagccagccg gtggccgctc gcggccatcg tccacatatc cacgggctgg
     5281 caagggagcg cagcgaccgc gcagggcgaa gcccggagag caagcccgta gggcgccgca
     5341 gccgccgtag gcggtcacga ctttgcgaag caaagtctag tgagtatact caagcattga
     5401 gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga gccggttgga caccaaaagg
     5461 gaggggcagg catggcggca tacgcgatca tgcgatgcaa gaagctggcg aaaatgggca
     5521 acgtggcggc cagtctcaag cacgcctacc gcgagcgcga gacgcccaac gctgacgcca
     5581 gcaggacgcc agagaacgag cactgggcgg ccagcagcac cgatgaagcg atgggccgac
     5641 tgcgcgagtt gctgccagag aagcggcgca aggacgctgt gttggcggtc gagtacgtca
     5701 tgacggccag cccggaatgg tggaagtcgg ccagccaaga acagcaggcg gcgttcttcg
     5761 agaaggcgca caagtggctg gcggacaagt acggggcgga tcgcatcgtg acggccagca
     5821 tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt ggtgccgctg acgcaggacg
     5881 gcaggctgtc ggccaaggag ttcatcggca acaaagcgca gatgacccgc gaccagacca
     5941 cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg catcgagggc agcaaggcac
     6001 gtcacacgcg cattcaggcg ttctacgagg ccctggagcg gccaccagtg ggccacgtca
     6061 ccatcagccc gcaagcggtc gagccacgcg cctatgcacc gcagggattg gccgaaaagc
     6121 tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc cgaccggctg acaaaagcgg
     6181 ttcggcaggg gtatgagcct gccctacagg ccgccgcagg agcgcgtgag atgcgcaaga
     6241 aggccgatca agcccaagag acggcccgag
//
