LOCUS       ___Agg-mNeonGree        7773 bp DNA     circular SYN 07-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     LOCUS                               7773 bp  DNA circular GCC  

            DEFINITION  CUP1-&acirc;&circ;&dagger;ss-CPYstar-mNeonGreen 

            ACCESSION   

            SOURCE      

              ORGANISM  

            COMMENT     This file was created using Genome Compiler.
COMMENT     Sequence Label: pCUP1-∆ssCPYstar-mNeonGreen pRS316
FEATURES             Location/Qualifiers
     source          1..7773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    190..410
                     /label=URA3 promoter
     misc_feature    411..1214
                     /label=URA3 ORF
                     /note="note=""URA3 ORF"" - 411..1214"
     misc_feature    2011..2421
                     /label=CUP1 promoter
                     /note="note=""CUP1 promoter"" - 2011..2421"
     primer_bind     2327..2356
                     /label=Primer 1
     misc_feature    2423..3958
                     /label=∆ssCPY*
                     /note="dnas_title=""CPY*"" - note="" - 2423..3958"
     misc_feature    3959..3964
                     /label=Linker2
                     /note="note=""A LINKER"" - 3959..3964"
     misc_feature    3965..4675
                     /label=mNeonGreen
     misc_feature    4682..4696
                     /label=Linker1
     misc_feature    4703..4977
                     /label=CYC terminator
                     /note="note=""CYC terminator"" - 4709..4983"
     primer_bind     complement(4781..4810)
                     /label=Primer 2
     misc_feature    5440..6028
                     /label=ori
     misc_feature    6199..7059
                     /label=AmpR
     misc_feature    7060..7164
                     /label=AmpR promoter
     misc_feature    7201..7704
                     /label=CEN/ARS
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     1981 tagggcgaat tggagctcca ccgcggtggc ggccgcatgt tcatgtatgt atctgtattt
     2041 aaaacacttt tgtattattt ttcctcatat atgtgtatag gtttatacgg atgatttaat
     2101 tattacttca ccacccttta tttcaggctg atatcttagc cttgttacta gttagaaaaa
     2161 gacatttttg ctgtcagtca ctgtcaagag attcttttgc tggcatttct tctagaagca
     2221 aaaagagcga tgcgtctttt ccgctgaacc gttccagcaa aaaagactac caacgcaata
     2281 tggattgtca gaatcatata aaagagaagc aaataactcc ttgtcttgta tcaattgcat
     2341 tataatatct tcttgttagt gcaatatcat atagaagtca tcgaaataga tattaagaaa
     2401 aacaaactgt aacgaattca tgatctcatt gcaaagaccg ttgggtctag ataaggacgt
     2461 tttgctgcaa gctgcggaaa aatttggttt ggacctcgac ctggatcatc tcttgaagga
     2521 gttggactcc aatgtattgg acgcttgggc ccaaatagag catttgtacc caaaccaggt
     2581 tatgagcctt gaaacttcca ctaagccaaa attccctgaa gcaatcaaaa cgaagaaaga
     2641 ctgggacttt gtggtcaaga atgacgcaat tgaaaactat cagcttcgtg tcaacaagat
     2701 taaggaccct aaaatcctgg gcattgaccc aaatgtcaca cagtacacgg gttacttgga
     2761 tgtggaagac gaggacaagc atttcttctt ttggactttt gaaagtagaa acgatcctgc
     2821 aaaggatccg gtcatccttt ggttgaacgg gggtccaggt tgttcttcac taaccgggct
     2881 gttctttgaa ttaggaccct catccattgg acctgatttg aaacccatcg ggaaccctta
     2941 ctcttggaac agcaatgcca ccgtgatctt ccttgaccag cctgtcaacg ttgggttctc
     3001 gtattccggg tcctcaggtg tttccaacac tgtcgccgct ggtaaggatg tctataactt
     3061 cttggagttg ttcttcgatc agttccctga atacgtcaac aagggccaag atttccacat
     3121 cgctagggaa tcctacgccg gccattacat ccctgttttt gcctctgaaa ttttgtctca
     3181 caaggacaga aacttcaact taacctccgt cttgatcgga aatggcctca ctgacccatt
     3241 gactcagtat aactattacg aaccaatggc ctgtggtgaa ggtggcgaac catctgtttt
     3301 gccctcggag gaatgctctg ctatggaaga ctctttggaa cgttgtttgg gcttgatcga
     3361 gtcgtgctat gactcgcaat ccgtctggtc ctgtgttcca gctaccattt attgtaataa
     3421 cgcccaattg gctccttacc aacgtaccgg cagaaacgtt tacgatatca ggaaggattg
     3481 tgaaggtggc aatttgtgct acccaacgtt acaagatatc gacgactact taaaccagga
     3541 ctacgtcaaa gaagctgtcg gtgcggaggt tgaccactac gaatcctgta acttcgatat
     3601 caacagaaat ttcctgtttg cgggtgattg gatgaagcct taccacaccg ccgtaacaga
     3661 tcttttgaat caagacctac ccattctggt atatgcaggc gataaagatt tcatctgtaa
     3721 ctggttgggt aataaggcgt ggacggatgt cttgccatgg aagtacgacg aagaatttgc
     3781 aagccaaaaa gtacgtaact ggactgcttc tatcaccgac gaggtcgctg gtgaagtcaa
     3841 atcctacaag cacttcacct atttgagagt cttcaatggt ggccacatgg ttccatttga
     3901 cgtccctgaa aacgccttaa gtatggttaa cgaatggatc cacggtggtt tctccttact
     3961 cgagatggtg agcaagggcg aggaggataa catggcctct ctcccagcga cacatgagtt
     4021 atacatcttt ggctccatca acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa
     4081 tccaaatgat ggttatgagg agttaaacct gaagtccacc aagggtgacc tccagttctc
     4141 cccctggatt ctggtccctc atatcgggta tggcttccat cagtacctgc cctaccctga
     4201 cgggatgtcg cctttccagg ccgccatggt agatggctcc ggctaccaag tccatcgcac
     4261 aatgcagttt gaagatggtg cctcccttac tgttaactac cgctacacct acgagggaag
     4321 ccacatcaaa ggagaggccc aggtgaaggg gactggtttc cctgctgacg gtcctgtgat
     4381 gaccaactcg ctgaccgctg cggactggtg caggtcgaag aagacttacc ccaacgacaa
     4441 aaccatcatc agtaccttta agtggagtta caccactgga aatggcaagc gctaccggag
     4501 cactgcgcgg accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca
     4561 gccgatgtac gtgttccgta agacggagct caagcactcc aagaccgagc tcaacttcaa
     4621 ggagtggcaa aaggccttta ccgatgtgat gggcatggac gagctgtaca agtagaagct
     4681 ttgccataga atatctggat ccggtaccgg gttgaagaag gaaaaagaca gaaacgactt
     4741 aattacctac ttgaaaaaag cctgtgagta aacaggcccc ttttcctttg tcgatatcat
     4801 gtaattagtt atgtcacgct tacattcacg ccctcctccc acatccgctc taaccgaaaa
     4861 ggaaggagtt agacaacctg aagtctaggt ccctatttat tttttttaat agttatgtta
     4921 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacaa acgcgtgtac
     4981 ggtacccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc
     5041 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg
     5101 aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt
     5161 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5221 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5281 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5341 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5401 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tcggcccccc
     5461 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5521 aagataccag gcgttccccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5581 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     5641 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5701 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5761 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5821 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5881 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5941 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6001 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6061 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6121 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6181 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6241 tctatttcgt tcatccatag ttgcctgact gcccgtcgtg tagataacta cgatacggga
     6301 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6361 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6421 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6481 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6541 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6601 catgttgtga aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6661 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6721 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6781 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6841 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6901 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6961 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7021 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7081 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7141 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtccttttc
     7201 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
     7261 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
     7321 actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat
     7381 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
     7441 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
     7501 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
     7561 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
     7621 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
     7681 cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat tattatcatg acattaacct
     7741 ataaaaatag gcgtatcacg aggccctttc gtc
//
