LOCUS       ____Agg-mScarlet        7761 bp DNA     circular SYN 06-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     LOCUS                               7761 bp  DNA circular GCC  

            DEFINITION  CUP1-&acirc;&circ;&dagger;ss-CPYstar-mScarlet I 

            ACCESSION   

            SOURCE      

              ORGANISM  

            COMMENT     This file was created using Genome Compiler.
FEATURES             Location/Qualifiers
     source          1..7761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    190..410
                     /label=URA3 promoter
     misc_feature    411..1214
                     /label=URA3 ORF
                     /note="note=""URA3 ORF"" - 411..1214"
     promoter        2011..2421
                     /label=CUP1 promoter
                     /note="note=""CUP1 promoter"" - 2011..2421"
     primer_bind     2327..2356
                     /label=Primer 1
     misc_feature    2423..3958
                     /label=∆ssCPY*
                     /note="dnas_title=""CPY*"" - note="" - 2423..3958"
     misc_feature    3959..3964
                     /label=Linker2
                     /note="note=""A LINKER"" - 3959..3964"
     misc_feature    3965..4663
                     /label=mScarlet-I
     misc_feature    4670..4684
                     /label=Linker1
                     /note="note=""CHRIS LINKER"" - 4688..4702"
     misc_feature    4691..4965
                     /label=CYC terminator
                     /note="note=""CYC terminator"" - 4709..4983"
     primer_bind     complement(4769..4798)
                     /label=Primer 2
     misc_feature    5428..6016
                     /label=ORI
     misc_feature    6187..7047
                     /label=AmpR
     misc_feature    7048..7152
                     /label=AmpR promoter
     misc_feature    7189..7692
                     /label=CEN/ARS
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     1981 tagggcgaat tggagctcca ccgcggtggc ggccgcatgt tcatgtatgt atctgtattt
     2041 aaaacacttt tgtattattt ttcctcatat atgtgtatag gtttatacgg atgatttaat
     2101 tattacttca ccacccttta tttcaggctg atatcttagc cttgttacta gttagaaaaa
     2161 gacatttttg ctgtcagtca ctgtcaagag attcttttgc tggcatttct tctagaagca
     2221 aaaagagcga tgcgtctttt ccgctgaacc gttccagcaa aaaagactac caacgcaata
     2281 tggattgtca gaatcatata aaagagaagc aaataactcc ttgtcttgta tcaattgcat
     2341 tataatatct tcttgttagt gcaatatcat atagaagtca tcgaaataga tattaagaaa
     2401 aacaaactgt aacgaattca tgatctcatt gcaaagaccg ttgggtctag ataaggacgt
     2461 tttgctgcaa gctgcggaaa aatttggttt ggacctcgac ctggatcatc tcttgaagga
     2521 gttggactcc aatgtattgg acgcttgggc ccaaatagag catttgtacc caaaccaggt
     2581 tatgagcctt gaaacttcca ctaagccaaa attccctgaa gcaatcaaaa cgaagaaaga
     2641 ctgggacttt gtggtcaaga atgacgcaat tgaaaactat cagcttcgtg tcaacaagat
     2701 taaggaccct aaaatcctgg gcattgaccc aaatgtcaca cagtacacgg gttacttgga
     2761 tgtggaagac gaggacaagc atttcttctt ttggactttt gaaagtagaa acgatcctgc
     2821 aaaggatccg gtcatccttt ggttgaacgg gggtccaggt tgttcttcac taaccgggct
     2881 gttctttgaa ttaggaccct catccattgg acctgatttg aaacccatcg ggaaccctta
     2941 ctcttggaac agcaatgcca ccgtgatctt ccttgaccag cctgtcaacg ttgggttctc
     3001 gtattccggg tcctcaggtg tttccaacac tgtcgccgct ggtaaggatg tctataactt
     3061 cttggagttg ttcttcgatc agttccctga atacgtcaac aagggccaag atttccacat
     3121 cgctagggaa tcctacgccg gccattacat ccctgttttt gcctctgaaa ttttgtctca
     3181 caaggacaga aacttcaact taacctccgt cttgatcgga aatggcctca ctgacccatt
     3241 gactcagtat aactattacg aaccaatggc ctgtggtgaa ggtggcgaac catctgtttt
     3301 gccctcggag gaatgctctg ctatggaaga ctctttggaa cgttgtttgg gcttgatcga
     3361 gtcgtgctat gactcgcaat ccgtctggtc ctgtgttcca gctaccattt attgtaataa
     3421 cgcccaattg gctccttacc aacgtaccgg cagaaacgtt tacgatatca ggaaggattg
     3481 tgaaggtggc aatttgtgct acccaacgtt acaagatatc gacgactact taaaccagga
     3541 ctacgtcaaa gaagctgtcg gtgcggaggt tgaccactac gaatcctgta acttcgatat
     3601 caacagaaat ttcctgtttg cgggtgattg gatgaagcct taccacaccg ccgtaacaga
     3661 tcttttgaat caagacctac ccattctggt atatgcaggc gataaagatt tcatctgtaa
     3721 ctggttgggt aataaggcgt ggacggatgt cttgccatgg aagtacgacg aagaatttgc
     3781 aagccaaaaa gtacgtaact ggactgcttc tatcaccgac gaggtcgctg gtgaagtcaa
     3841 atcctacaag cacttcacct atttgagagt cttcaatggt ggccacatgg ttccatttga
     3901 cgtccctgaa aacgccttaa gtatggttaa cgaatggatc cacggtggtt tctccttact
     3961 cgagatggtc tccaaaggag aggccgtaat caaagagttc atgagattca aagttcacat
     4021 ggaggggtct atgaacggcc atgagttcga gatagaagga gagggcgaag ggcggcctta
     4081 cgaaggtacg cagactgcta aattgaaagt taccaaaggt ggcccacttc catttagttg
     4141 ggatatttta agcccacaat tcatgtatgg gtccagagct ttcatcaaac accctgctga
     4201 tatacctgac tattacaagc agagcttccc agaagggttc aagtgggaaa gagtaatgaa
     4261 ctttgaagac ggaggagctg tcaccgtgac gcaggatacc agtcttgaag acggcacgct
     4321 gatttataag gtcaagctca gagggacaaa ttttcctcct gacgggcctg ttatgcagaa
     4381 aaaaacgatg ggttgggagg catcaacgga gcgtctctat ccagaggacg gtgttttaaa
     4441 gggagatatc aaaatggcac tccgtctcaa ggatggaggc agatacctcg ctgactttaa
     4501 gaccacatac aaagcgaaaa aacctgttca aatgccaggg gcgtataacg tggatcgtaa
     4561 gctggacatt accagccata acgaagatta cacagtggtg gagcagtatg agagaagtga
     4621 aggccgccat tccacgggtg gtatggatga gctgtataaa taaaagcttt gccatagaat
     4681 atctggatcc ggtaccgggt tgaagaagga aaaagacaga aacgacttaa ttacctactt
     4741 gaaaaaagcc tgtgagtaaa caggcccctt ttcctttgtc gatatcatgt aattagttat
     4801 gtcacgctta cattcacgcc ctcctcccac atccgctcta accgaaaagg aaggagttag
     4861 acaacctgaa gtctaggtcc ctatttattt tttttaatag ttatgttagt attaagaacg
     4921 ttatttatat ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgg tacccagctt
     4981 ttgttccctt tagtgagggt taattccgag cttggcgtaa tcatggtcat agctgtttcc
     5041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa gcataaagtg
     5101 taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc
     5161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc ggcccccctg acgagcatca
     5461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5521 gttcccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     5641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     5881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5941 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6001 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6121 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6181 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6241 atccatagtt gcctgactgc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6301 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6361 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6421 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6481 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6541 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgaaa
     6601 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6661 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6721 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6781 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6841 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6901 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6961 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7021 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7081 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7141 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat
     7201 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa
     7261 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga
     7321 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt
     7381 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt
     7441 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta
     7501 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta
     7561 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa
     7621 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac
     7681 aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     7741 gtatcacgag gccctttcgt c
//
