LOCUS       pCUP1-∆ssCPYstar        8895 bp DNA     circular SYN 08-JUN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     LOCUS                               7776 bp  DNA circular GCC 
            DEFINITION  CUP1-&acirc;&circ;&dagger;ss-CPYstar-mGFP (p695) 
            ACCESSION
            SOURCE
              ORGANISM
            COMMENT     This file was created using Genome Compiler.
COMMENT     Sequence Label: pCUP1-Delta-ssCPY*-mGFP pRS315
FEATURES             Location/Qualifiers
     source          1..8895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3554..5089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    663..1757
                     /label=LEU2 ORF
                     /note="LEU2 ORF"
     promoter        1770..2174
                     /label=LEU2 promoter
                     /note="LEU2 promoter"
     promoter        3142..3552
                     /label=CUP1 promoter
                     /note="note=""CUP1 promoter"" - 2011..2421"
     primer_bind     3458..3487
                     /label=Primer 1
     primer_bind     3458..3487
                     /label=Primer 1
     misc_feature    3554..5089
                     /label=∆ssCPY*
                     /note="dnas_title=""CPY*"" - note="" - 2423..3958"
     misc_feature    5090..5095
                     /label=Linker2
                     /note="note=""A LINKER"" - 3959..3964"
     misc_feature    5096..5809
                     /label=mGFP
                     /note="note=""eGFP F64L / G65T / A72S / Q80R / A206V"" - 
                     3965..4678"
     misc_feature    5816..5830
                     /label=Linker1
                     /note="note=""CHRIS LINKER"" - 4688..4702"
     misc_feature    5837..6111
                     /label=CYC terminator
                     /note="note=""CYC terminator"" - 4709..4983"
     primer_bind     complement(5915..5944)
                     /label=Primer 2
     primer_bind     complement(5915..5944)
                     /label=Primer 2
     promoter        6133..6151
                     /label=T7 promoter
     misc_feature    6574..7162
                     /label=ori
     misc_feature    7333..8193
                     /label=AmpR
     promoter        8194..8286
                     /label=AmpR promoter
                     /label=Amp promoter
     misc_feature    8323..8826
                     /label=CEN/ARS
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
     2401 gaagttctcc tcgagggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3001 cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3061 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     3121 ttggagctcc accgcggtgg cggccgcatg ttcatgtatg tatctgtatt taaaacactt
     3181 ttgtattatt tttcctcata tatgtgtata ggtttatacg gatgatttaa ttattacttc
     3241 accacccttt atttcaggct gatatcttag ccttgttact agttagaaaa agacattttt
     3301 gctgtcagtc actgtcaaga gattcttttg ctggcatttc ttctagaagc aaaaagagcg
     3361 atgcgtcttt tccgctgaac cgttccagca aaaaagacta ccaacgcaat atggattgtc
     3421 agaatcatat aaaagagaag caaataactc cttgtcttgt atcaattgca ttataatatc
     3481 ttcttgttag tgcaatatca tatagaagtc atcgaaatag atattaagaa aaacaaactg
     3541 taacgaattc atgatctcat tgcaaagacc gttgggtcta gataaggacg ttttgctgca
     3601 agctgcggaa aaatttggtt tggacctcga cctggatcat ctcttgaagg agttggactc
     3661 caatgtattg gacgcttggg cccaaataga gcatttgtac ccaaaccagg ttatgagcct
     3721 tgaaacttcc actaagccaa aattccctga agcaatcaaa acgaagaaag actgggactt
     3781 tgtggtcaag aatgacgcaa ttgaaaacta tcagcttcgt gtcaacaaga ttaaggaccc
     3841 taaaatcctg ggcattgacc caaatgtcac acagtacacg ggttacttgg atgtggaaga
     3901 cgaggacaag catttcttct tttggacttt tgaaagtaga aacgatcctg caaaggatcc
     3961 ggtcatcctt tggttgaacg ggggtccagg ttgttcttca ctaaccgggc tgttctttga
     4021 attaggaccc tcatccattg gacctgattt gaaacccatc gggaaccctt actcttggaa
     4081 cagcaatgcc accgtgatct tccttgacca gcctgtcaac gttgggttct cgtattccgg
     4141 gtcctcaggt gtttccaaca ctgtcgccgc tggtaaggat gtctataact tcttggagtt
     4201 gttcttcgat cagttccctg aatacgtcaa caagggccaa gatttccaca tcgctaggga
     4261 atcctacgcc ggccattaca tccctgtttt tgcctctgaa attttgtctc acaaggacag
     4321 aaacttcaac ttaacctccg tcttgatcgg aaatggcctc actgacccat tgactcagta
     4381 taactattac gaaccaatgg cctgtggtga aggtggcgaa ccatctgttt tgccctcgga
     4441 ggaatgctct gctatggaag actctttgga acgttgtttg ggcttgatcg agtcgtgcta
     4501 tgactcgcaa tccgtctggt cctgtgttcc agctaccatt tattgtaata acgcccaatt
     4561 ggctccttac caacgtaccg gcagaaacgt ttacgatatc aggaaggatt gtgaaggtgg
     4621 caatttgtgc tacccaacgt tacaagatat cgacgactac ttaaaccagg actacgtcaa
     4681 agaagctgtc ggtgcggagg ttgaccacta cgaatcctgt aacttcgata tcaacagaaa
     4741 tttcctgttt gcgggtgatt ggatgaagcc ttaccacacc gccgtaacag atcttttgaa
     4801 tcaagaccta cccattctgg tatatgcagg cgataaagat ttcatctgta actggttggg
     4861 taataaggcg tggacggatg tcttgccatg gaagtacgac gaagaatttg caagccaaaa
     4921 agtacgtaac tggactgctt ctatcaccga cgaggtcgct ggtgaagtca aatcctacaa
     4981 gcacttcacc tatttgagag tcttcaatgg tggccacatg gttccatttg acgtccctga
     5041 aaacgcctta agtatggtta acgaatggat ccacggtggt ttctccttac tcgagagtaa
     5101 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     5161 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     5221 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     5281 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
     5341 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     5401 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     5461 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     5521 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     5581 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     5641 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     5701 acaatctaaa ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     5761 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataaa agctttgcca
     5821 tagaatatct ggatccggta ccgggttgaa gaaggaaaaa gacagaaacg acttaattac
     5881 ctacttgaaa aaagcctgtg agtaaacagg ccccttttcc tttgtcgata tcatgtaatt
     5941 agttatgtca cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg
     6001 agttagacaa cctgaagtct aggtccctat ttattttttt taatagttat gttagtatta
     6061 agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacggtacc
     6121 cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct
     6181 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat
     6241 aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc
     6301 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctcggcc cccctgacga
     6601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6661 ccaggcgttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     6781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7381 tcgttcatcc atagttgcct gactgcccgt cgtgtagata actacgatac gggagggctt
     7441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7621 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7741 gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7921 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgagg
     8221 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8281 tccgcgcaca tttccccgaa aagtgccacc tgggtccttt tcatcacgtg ctataaaaat
     8341 aattataatt taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga
     8401 aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg gggatcgcca
     8461 acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg aattgtttca
     8521 tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt acttatcgtt
     8581 aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata tgtaaagtac
     8641 gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc cgtaactctt
     8701 ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa ataaacacag
     8761 agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag ttacaggcaa
     8821 gcgatccgtc ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8881 cgaggccctt tcgtc
//
