LOCUS       Authentication_Report_ 12265 bp ds-DNA     circular     18-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     35..55
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    92..97
                     /label="MluI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    98..5594
                     /label="Sox17"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3454..3454
                     /label="c>t SNP"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4096..4096
                     /label="This bp is missing in pNU3407 Sanger and nanopore sequence"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5594..5599
                     /label="SalI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5600..5608
                     /label="Kozac (IVB)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    5609..6412
                     /label="EMTB"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             5609..8689
                     /label="EMTB-3xEGFP"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /translation="MAPVRSETAPDSYKVQDKKNASSRPASAISGQNNNHSGNKPDPPPVLRVDDRQRLARERREEREKQLAAREIVWLEREERARQHYEKHLEERKKRLEEQRQKEERRRAAVEEKRRQRLEEDKERHEAVVRRTMERSQKPKQKHNRWSWGGSLHGSPSIHSADPDRRSVSTMNLSKYVDPVISKRLSSSSATLLNSPDRARRLQLSPWESSVVNRLLTPTHSFLARSKSTAALSGEAASCSPIIMPYKAAHSRNSMDRPKLFVTPPEGSSDPPVATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGPRDIPEGSSDPPVATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKESGHGTPRARDPPVATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     regulatory      6428..6437
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6434..8688
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      7199..7208
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7964..7973
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8715..8720
                     /label="BamHI site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    8720..8911
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     polyA_signal    8977..9168
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    9229..9271
                     /label="three"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     misc_feature    complement(9229..9563)
                     /label=">Tol2_exon1,_bp1-335_(of_461)"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     misc_feature    9229..9745
                     /label="Tol2 L"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    9707..9745
                     /label="four"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     misc_feature    complement(9727..9745)
                     /label=">5'_TIR"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(9946..10806)
                     /label="AmpR "
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      10901..11566
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      11567..11583
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    11753..23
                     /label="Tol2 R"
                     /ApEinfo_revcolor="#a2d39c"
                     /ApEinfo_fwdcolor="#a2d39c"
     misc_feature    11753..11769
                     /label=">3'_TIR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    11753..11798
                     /label="one"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     misc_feature    complement(11942..23)
                     /label=">Tol2_exon4_136-482"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     misc_feature    12249..23
                     /label="two"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
ORIGIN
        1 GGTGTCTGAA ACACAGGCCA GATGGGCCCT CGAGCAGGAA ACAGCTATGA CCATGATTAC
       61 GCCAAGCTAT CAACTTTGTA TAGAAAAGTT GACGCGTatg tggttgagaa acactgctct
      121 agagcatttt agactggttt ttaacctgtc ttctcttttt ggcaatatga tagatgagtt
      181 tgggtatgga ggttgccagg agaatgggac ttgcctgact gcattgtgcc aattggtatg
      241 tttggtggtg gataatggtg taagattgtt tttcaggtgt tggtccctta gttctgacag
      301 aacgacttag gaggcagata aacatcttat tatgaattgt tttggcaaat tcaagtatgt
      361 caactatgat tcaggtcttg gcatgagcct aaatgatgaa tgcagggcag agacgacccc
      421 caggtcaacc tacactctct gacaaaaggt acacggtagg acaaaaaatg ttctataagg
      481 aacagttttg tacctttgta aaagttgtac cttgtttgaa aaaagacctc ttatgatact
      541 tttctgtact ttttatggta caactgaaat aaaggtacac aattgttttc ataaaacagt
      601 aaataagtgt tggacaactc cttctttgtt acaatatcca tgaaaataaa tattactgga
      661 aaggcaaagt gatttatgaa taagtgaagt ttatttagaa actaatttca agaggatcac
      721 atgcttatga ttgcttggtc ccacaatatc caatttatga tttaccattc agacgattcc
      781 taagccacta taattacaat aagttccata tcacagtcat cttcattttg aagaatccct
      841 ccttccaccc ctactactcc tcctttccta gatgggtggc acagtcgccc agtggtttgc
      901 actgttgcct cacagcaaga acgtcactgg ttctagacct taccaagcca gatgagtttc
      961 tgtgcaagtt tacacattct ccttatgtgc tcatgtgggt ttcccacagg ttcccccgtt
     1021 tcctcccacc gtccaaaaac atgcaactta atttaataga ctaatccaaa taggcacctt
     1081 agacattctc ctaacatgct ccgaacttat ctcttaagag taatcactat ttgttcatta
     1141 gctacagcag gggagttctc gagatctacc tgagctcaga cttgcctctc accttgcaaa
     1201 cgggagggag ccccaggctt gaggatcgga tgagctcagg gctctctccc gggacagcat
     1261 gccaaacacg ctttataatc aatcatcagc taagtgtgaa cacctgaact tccgcattat
     1321 tacatgaatc atcatttaaa atcatatgtt taaagaaact cataaacatt aattattaat
     1381 ttctataata caaaaccact ggaaaaacat ataactatag gtattgtaaa ctaaacattt
     1441 agggcatttt taatatacac ttttattaaa cattttctga tagatccctt ttcattattg
     1501 atatccacta tcaatttgct ttccactaag caagtgagct ggcaaaagtc ctgtacatgc
     1561 aaagtgttca tgcagacatt ttagtactgt aaaactaatc aaacattgtg caaatctttt
     1621 tacaatactt ttgcgtaatt ctatttctat ttcaaaatta agtaaattaa attaacttta
     1681 aagtgacttt aaaggtatta tgtgaaaatt agagtttttt ttattgtaca tgggagaatg
     1741 tttagcaaaa aacagatttt gatttatgtt aaagtttttt ttaaattctt tattgtaaat
     1801 ggaagaggtg catttacaca aaaataaact ttttttaaaa cattgtttaa taaatgaatt
     1861 agctgtttta tatttactga aaataaatta acccatttta gatatagcaa aaaggcttta
     1921 aatagttctt gaaggaacac atttgacact gttaaggtac aaagtgtctt gttactgtag
     1981 tggtaccttc atggatcgga tttgtacctt tgatgaaggt acaatattgt atttacaggt
     2041 tttaaaaagc caaaggtaca ttttgatttc ctatggtaca aatcatgtcc ttaaatgtac
     2101 aaactgcaat agtacaaaat tgttttttgt cttacggtac aactctgttc cataaaaaag
     2161 tgttgcccca gtgacaaggg tttgtacatt ctttagtact acattctacc atttttttct
     2221 gagagtgtat ggacagagaa atcgattgtt taaatatatg tatatatagc gtgctttgtt
     2281 gtcatatagg ctgaagggat aattattatg aataactaaa ttagattaga ttctttgcat
     2341 tagattcttc ctcgtagacc tttcgttgta gctttaactt gcactgaaag gaactattta
     2401 cccttggatg atcttagaat tctttatttt ctgtttgttc tatgggtgaa aaatgacccg
     2461 tattcactaa acaccttcac taaagcaaca caattttatt taaattctca gatccaattt
     2521 gcacaaagaa acgacctcat cattcatcag ctaccctttt aatgactggg tttttccatg
     2581 ttggggtgta gtttgtcatc aatgttcaat caactggcac cacaattttt ttcttcattc
     2641 attatccgac ggctttgtcc ctttatttat cagggatcac cacagcagaa tgaaccgcca
     2701 acttatttaa catatgtttt acacagcaga tgcccttcca gatgcaacct agtacagaga
     2761 aacgcccatg cactcacatt cccactcata cactatggcc aatttagttt attcaattca
     2821 cctatagcgc atgtgtttgg actgtggggg aaaccagagc acgcggagga aactcgcgca
     2881 aacatgggga aaacatgcaa actctataca gaaatgtcaa ctgacccagc cgggactcga
     2941 actagtgacc ttcttgctgt aaggcaacag tgctaaccac tgagccactg tgttgcccca
     3001 ccactcggtt ttgactgaag taaaggttta aaaagtatac atttctaaat ctaaatctgt
     3061 acagccttca ttaaaataga gaattgttct ttattttata atgctgcttc attttagaaa
     3121 aaaaatcata atacttcata atatatattt taaataaaat aataatataa tatatatttt
     3181 aaaatggtta tggctcacta ttatttgctg ttttaagtac ttgagtacag tcaacccgcc
     3241 tctgaatgcc ttcagcctgc cttaccctaa agcggattcc ctgatttctc catccatcat
     3301 ccacctcctc tgtttggagt tttatctgcc tgccatttga tctgcccatt caattgtttc
     3361 tgtttgttct actgactctg accctcgctg acttcagcac aagcctgtcc tttctggttc
     3421 tgcctgcctt tcgtgcctaa aacttttttg acctggctga caaatattta cagtctgacc
     3481 ttaccaagtg gtacagtctt ctttgttttt ggttaataaa tcagatgtta tttaacctgt
     3541 acatgagctc actctctccc cactgccctc aagactacca tatcccgtca ggtatttata
     3601 actttttttg aatgctaaca tgtgaggcac cactaaggcc taggggagtc tgggatggag
     3661 tcaggggtgg gtgggtgggt gtacctactt aaccatattt tgtaagcata tttgcacctc
     3721 cctacaggga tgtctgtatt tgtaaaaaca aaatagaata aaaatagcac agctcttatt
     3781 attgaaaatg cccaaaatgc ctaaaatggg acttttgggg ggttaaactt tagtgtttat
     3841 aattagctga gtaaaagcat ttaaaaacgt cccgtttaga gaagaaaacg tgtgtgtgtt
     3901 ttgtatatgg gagaggtctt ccacccttat ctacgctata gtgcttcagc ctgcatttcc
     3961 tgtcccccac aaactgcttc ctgtccttgg cgttggagca catactgtcc gccttcagac
     4021 aaacacaccc tctccctcac agtcaaatct gcacatagac tcacactcat gaaaatacac
     4081 acatactgtc gccagtgcaa gcctttgtgg ggaattacac aatagcattg gctcctgagt
     4141 gccaggggac tttacatgag tcagacggaa gttgtatatg cgttcacact caccacttac
     4201 acacgcatgg tccattgtgg tccatttgtt cttttggagt gaaatagatg atgtgcgagc
     4261 tatatgtcag aactcagtga tttggatgaa tctagaatct gcattacagt gactccaaac
     4321 caacactaaa aaataacttc tattaaaaag tttctaaaga agtttctcta aaaacttcta
     4381 ttaagcatac actcctgtgt aaaaattcaa atcaagataa gcagaaaata ggatgtggtt
     4441 ttcgtagttc ccaataattc ctgtaaccct gcacaggagc ttttagttct caaaacattt
     4501 acaacttacc tgcatataca gccttcatga taacaagcca ctgcaaactt tatgttagtg
     4561 attctgtcac atggattaca acagtttgac aacagcagaa atgtcagtct acagtattca
     4621 ataagtgttt ggtttatttt tttatttaaa tcataattca gtaaacctat taaactcctt
     4681 caaagcttat tttactttag aataaagatc agactcaaaa ctgcttttta ttatgacctc
     4741 ctcaaagtaa tttagtagag aaactggcat tgtagcttct actaaataca tcatatacaa
     4801 agttaacacc ttcttttcac acgcatttgg cacgccgtta tcaacgtgtg acaatctttc
     4861 acctactagc ctactactgt ttgcaaattc aggctgtatc tactggcagg atattaatcc
     4921 taaaccaaac gttgtatttg tcgtatccgt gtggcaaata tttaaacctt cacttgctat
     4981 gataaacgtt accaacattt aaatcgcttt agtttcaaac gaaaatagat aaacctttaa
     5041 actcgtcctt taatgccaaa aaagtagaat tataaagttt ggagaagttc atgaatgctt
     5101 ccgtatatag attggacgct gttcttgcaa tgtgtttttg acaataaata aagaaagaaa
     5161 gccgtcttaa cgaaactgtg tgcttcttaa agatcaatga cagatgggca aaacatgcca
     5221 atggttgcga acgcttatca ctttacttgt tataggcaac gcagcagatt ttgtagctta
     5281 aatgttttaa ataagagaac cttcctggaa agcagaattt ctaatattgt cgtccagatg
     5341 atgataggct ctacacaaga actgattctt tcacaccttt taactgcgcg gtccgcccac
     5401 attagcgcta ttggtggctg ctgagtttaa agctgcctga aactttcact gtggattttc
     5461 tgacctttca tgttgaatga actgtatgca ctcagcaaag gacagttgag cagaggaaaa
     5521 tttaaaagat cagcttttag gccattcagg tttcatcggt gatcaataag gatacgcgcc
     5581 cggtgcgtgt ttggtcgacg ccgccaccAT GGCGCCAGTG CGAAGCGAAA CAGCACCCGA
     5641 CAGCTACAAA GTGCAAGATA AGAAAAATGC CTCCAGCCGC CCTGCCTCTG CAATTTCAGG
     5701 ACAAAATAAC AACCACTCAG GAAATAAACC AGACCCTCCG CCTGTGTTAC GTGTTGATGA
     5761 CCGGCAGCGG CTGGCCCGGG AGCGACGTGA GGAACGGGAG AAACAGCTAG CTGCAAGAGA
     5821 AATAGTGTGG TTAGAAAGAG AAGAGCGAGC CAGGCAGCAC TACGAGAAGC ACCTGGAAGA
     5881 GCGGAAGAAG AGGTTGGAGG AGCAGAGGCA GAAGGAGGAG CGGAGGAGGG CTGCTGTGGA
     5941 GGAGAAGCGG AGGCAGAGAC TTGAGGAGGA CAAAGAACGC CACGAAGCTG TTGTACGGCG
     6001 CACAATGGAA AGGAGCCAGA AGCCAAAACA GAAGCATAAC CGTTGGTCGT GGGGAGGCTC
     6061 TCTCCATGGG AGCCCTAGCA TCCACAGTGC AGATCCAGAC AGGCGGTCAG TTTCCACCAT
     6121 GAATCTTTCG AAATATGTTG ATCCCGTCAT TAGCAAGCGG CTCTCCTCTT CATCTGCAAC
     6181 TTTACTAAAT TCTCCAGATA GAGCTCGCCG CCTGCAGCTC AGCCCATGGG AGAGCAGCGT
     6241 TGTTAACAGA CTCCTGACGC CCACACATTC GTTCCTGGCC AGAAGTAAAA GCACAGCTGC
     6301 CTTGTCTGGA GAAGCAGCAT CTTGCAGCCC CATCATCATG CCCTACAAAG CTGCACACTC
     6361 TAGAAATTCG ATGGATCGAC CAAAACTCTT TGTAACACCA CCTGAGGGCT CTTCGGATCC
     6421 ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT
     6481 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG
     6541 CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT
     6601 GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC
     6661 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA
     6721 GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA
     6781 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA
     6841 CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA
     6901 CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG
     6961 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
     7021 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
     7081 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
     7141 GCTGTACAAG GGAAGTGGAC CGCGGGATAT CCCTGAGGGC TCTTCGGATC CACCGGTCGC
     7201 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
     7261 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
     7321 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
     7381 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT
     7441 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
     7501 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
     7561 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
     7621 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA
     7681 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT
     7741 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
     7801 CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT
     7861 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA
     7921 GGAAAGCGGC CACGGTACCC CGCGGGCCCG GGATCCACCG GTCGCCACCA TGGTGAGCAA
     7981 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
     8041 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
     8101 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
     8161 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
     8221 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
     8281 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
     8341 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
     8401 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
     8461 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA
     8521 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC
     8581 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
     8641 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAA CCCAGCTTTC
     8701 TTGTACAAAG TGGGGGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA
     8761 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT
     8821 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT
     8881 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TCCAACTTTA TTATACATAG TTGATAATTC
     8941 ACTGGCCGTC GTTTTACGGT ACCATCGATG ATGATCCAGA CATGATAAGA TACATTGATG
     9001 AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG
     9061 ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT
     9121 GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA
     9181 ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGA TCAGATCTGC GAAGATACGG
     9241 CCACGGGTGC TCTTGATCCT GTGGCTGATT TTGGACTGTG CTGCTCGCAG CTGCTGATGA
     9301 ATCACATACT TCCTCCATTT TCTTCCACTG ATTGACTGTT ATAATTTCCC TAATTTCCAG
     9361 GTCAAGGTGC TGTGCATTGT GGTAATAGAT GTGACATGAC GTCACTTCCA AAGGACCAAT
     9421 GAACATGTCT GACCAATTTC ATATAATGTG AAAACGATTT TCATAGGCAG AATAAATAAC
     9481 ATTTAAATTA AACTGGGCAT CAGCGCAATT CAATTGGTTT GGTAATAGCA AGGGAAAATA
     9541 GAATGAAGTG ATCTCCAAAA AATAAGTACT TTTTGACTGT AAATAAAATT GTAAGGAGTA
     9601 AAAAGTACTT TTTTTTCTAA AAAAATGTAA TTAAGTAAAA GTAAAAGTAT TGATTTTTAA
     9661 TTGTACTCAA GTAAAGTAAA AATCCCCAAA AATAATACTT AAGTACAGTA ATCAAGTAAA
     9721 ATTACTCAAG TACTTTACAC CTCTGGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG
     9781 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG
     9841 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA
     9901 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT
     9961 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA
    10021 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC
    10081 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA
    10141 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG
    10201 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA
    10261 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA
    10321 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG
    10381 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG
    10441 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA
    10501 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA
    10561 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT
    10621 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GATCTCGCGG TATCATTGCA
    10681 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG
    10741 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT
    10801 TGGTAActgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcTTGA
    10981 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
    11041 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
    11101 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
    11161 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
    11221 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
    11281 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
    11341 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
    11401 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
    11461 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
    11521 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAaacgc cagcaacgcg
    11581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg ccCAGAGGTG
    11761 TAAAAAGTAC TCAAAAATTT TACTCAAGTG AAAGTACAAG TACTTAGGGA AAATTTTACT
    11821 CAATTAAAAG TAAAAGTATC TGGCTAGAAT CTTACTTGAG TAAAAGTAAA AAAGTACTCC
    11881 ATTAAAATTG TACTTGAGTA TTAAGGAAGT AAAAGTAAAA GCAAGAAAGA AAACTAGAGA
    11941 TTCTTGTTTA AGCTTTTAAT CTCAAAAAAC ATTAAATGAA ATGCATACAA GGTTTTATCC
    12001 TGCTTTAGAA CTGTTTGTAT TTAATTATCA AACTATAAGA CAGACAATCT AATGCCAGTA
    12061 CACGCTACTC AAAGTTGTAA AACCTCAGAT TTAACTTCAG TAGAAGCTGA TTCTCAAAAT
    12121 TGTTAGTGTC AAGCCTAGCT CTTTTGGGGC TGAAAAGCAA TCCTGCAGTG CTGAAAAGCC
    12181 TCTCACAGGC AGCCGATGCG GGAAGAGGTG TATTAGTCTT GATAGAGAGG CTGCAAATAG
    12241 CAGGAAACGT GAGCAGAGAC TCCCT
//