LOCUS       pTE5417_[p3WJdB_Promoter-Dropout]        3139 bp    DNA     circular UNA 06-MAY-2024
DEFINITION  Promoter dropout for 3WJdB reporter plasmid, 5' connector: 5C1SI,
            Promoter+RBS Dropout (sfGFP Expression cassette), Reporter: 3WJdB,
            Terminator: T7, 3' connector: 3C1RSI, AmpR, ColE1, Lvl1 plasmid.
ACCESSION   urn.local.barthels.2h-hoqmyp3
VERSION     urn.local.barthels.2h-hoqmyp3
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     unsure          1..4
                     /note="/created_by=Daniel"
                     /note="/modified_by=Daniel"
                     /label="Prom overhang"
     misc_feature    12..141
                     /label="BBa_J72163 GlpT Promoter"
     misc_feature    142..177
                     /label="sfGSP_RBS"
     CDS             178..891
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/16369541""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/16369541"">Péde
                     lacq et al., 2006</a>)"
                     /transl_table=1
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
                     /label="superfolder GFP"
     misc_feature    895..1023
                     /label="BBa_B0015 Terminator"
     terminator      903..974
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /label="rrnB T1 terminator"
     terminator      990..1017
                     /note="phage T7 early transcription terminator"
                     /label="T7Te terminator"
     misc_feature    1031..1034
                     /modified_by="BarthelS"
                     /label="CDS overhang"
     misc_feature    1035..1186
                     /label="3WJdB"
     terminator      1193..1239
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /label="T7 terminator"
     unsure          1240..1243
                     /note="/created_by=Daniel"
                     /label="3`Connector Overhang"
     terminator      complement(1267..1363)
                     /label="AmpR Terminator"
     CDS             complement(1364..2221)
                     /label="AmpR"
     primer_bind     complement(1369..1391)
                     /Sequence="GATAGGTGCCTCACTGATTAAGC"
                     /Tm="58.9"
                     /%GC="47.8"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /created_by="BarthelS"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oSB0022_lvl1_seq_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:sbarthel:15u-e1a0lzy"">oSB0022_lvl1_seq_r
                     ev</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:sbarthel:15u-e1a0lzy"">oSB0022_lvl1_seq_r
                     ev</a>"
                     /label="oSB0022_lvl1_seq_rev"
     promoter        complement(2222..2352)
                     /label="AmpR Promoter"
     rep_origin      complement(2353..3116)
                     /snapgene_direction="LEFT"
                     /label="ColE1"
     primer_bind     3042..3059
                     /Sequence="CGGTTCCTGGCCTTTTGC"
                     /Tm="59.3"
                     /%GC="61.1"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="3.3"
                     /created_by="BarthelS"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oSB0021_lvl1_seq_fwd"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:sbarthel:15n-e1a0lzy"">oSB0021_lvl1_seq_f
                     wd</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:sbarthel:15n-e1a0lzy"">oSB0021_lvl1_seq_f
                     wd</a>"
                     /label="oSB0021_lvl1_seq_fwd"
     unsure          3125..3128
                     /note="/created_by=Daniel"
                     /note="/modified_by=Daniel"
                     /label="5`Connector Überhang"
ORIGIN      
        1 ggagtgagac cgaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt
       61 atttaataat gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc
      121 aactcaagaa acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg
      181 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
      241 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
      301 ctgacgctga agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta
      361 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
      421 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
      481 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
      541 cgcattgagc tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg
      601 gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt
      661 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
      721 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
      781 agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
      841 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc
      901 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      961 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1021 ataggtctct aatgcccaca tactctgatg atccgagacg gtcgggtcca gatattcgta
     1081 tctgtcgagt agagtgtggg ctcggatcat tcatggcaag agacggtcgg gtccagatat
     1141 tcgtatctgt cgagtagagt gtgggctctt gccatgtgta tgtggggctt aatagcataa
     1201 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc gcttgttgga gacgagctgc
     1261 ggccgcgatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1321 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1381 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1441 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1501 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1561 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1621 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1681 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1741 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1801 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1861 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1921 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1981 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2041 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2101 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2161 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2221 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2281 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2341 aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg
     3121 ccgcaacacg tctcgagta
//
