LOCUS       DC646-tadA_BLM        8480 bp DNA     circular SYN 16-JUL-2023
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...as2-e7pntkb
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 14, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          393..395
                     /note="organism: synthetic DNA construct mol_type: other
                     DNA"
     source          396..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        132..335
                     /standard_name="CMV promoter"
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     286..306
                     /standard_name="CMV-F"
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     332..356
                     /standard_name="LNCX"
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    complement(347..366)
                     /direction=LEFT
                     /standard_name="Express R"
                     /label=nonstandard type: primer_bind_reverse
     CDS             390..392
                     /standard_name="AID"
                     /label=AID
     CDS             393..395
                     /standard_name="Translation 90-686"
                     /label=Translation 90-686
     misc_feature    complement(393..395)
                     /standard_name="68"
                     /label=68
     misc_feature    393..395
                     /standard_name="overlap"
                     /label=overlap
     misc_feature    393..395
                     /standard_name="61"
                     /label=61
     misc_feature    393..394
                     /standard_name="GACAGCCTCTTGATGAACCGGAGGGAGTTTCTTTACCAATTC 
                     AAAAATGTCCGCTGGGCTAAGGGTCGGCGTGAGACCTACCTGTGCTACGTAGTGAAGA 
                     GGCGTGACAGTGCTACATCCTTTTCACTGGACTTTGGTTATCTTCGCAATAAGAACGG 
                     CTGCCACGTGGAATTGCTCTTCCTCCGCTACATCTCGGACTGGGACCTAGACCCTGGC 
                     CGCTGCTACCGCGTCACCTGGTTCATCTCCTGGAGCCCCTGCTACGACTGTGCCCGAC 
                     ATGTGGCCGACTTTCTGCGAGGGAACCCCAACCTCAGTCTGAGGATCTTCACCGCGCG 
                     CCTCTACTTCTGTGAGGACCGCAAGGCTGAGCCCGAGGGGCTGCGGCGGCTGCACCGC 
                     GCCGGGGTGCAAATAGCCATCATGACCTTCAAAGATTATTTTTACTGCTGGAATACTT 
                     TTGTAGAAAACCACGGAAGAACTTTCAAAGCCTGGGAAGGGCTGCATGAAAATTCAGT 
                     TCGTCTCTCCAGACAGCTTCGGCGCATCCTTTTGCCCCTGTATGAGGTTGATGACTTA 
                     CGAGACGCATTTCGTAC"
                     /label=nonstandard type: Editing History Replacement
     misc_feature    396..893
                     /standard_name="ins1 >pDL5"
                     /label=nonstandard type: ins1
     misc_feature    396..893
                     /label=nonstandard type: Editing History Insertion
     CDS             396..893
                     /codon_start=1
                     /standard_name="TadA(ABE8e)"
                     /label=TadA(ABE8e)
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQS
                     SIN"
     primer_bind     complement(924..943)
                     /standard_name="primer 1"
                     /label=primer 1
     primer_bind     942..966
                     /standard_name="BLM_Fwd"
                     /label=BLM_Fwd
     misc_feature    942..944
                     /standard_name="T7 RNA Polymerase G645A (concatenated
                     sequence 2)"
                     /label=nonstandard type: Concatenated sequence
     misc_feature    943..5192
                     /standard_name="ATCAGACATAGAGCTCGCGGCTATTCCGTTCAACACCCTGGC 
                     TGACCACTACGGCGAGAGACTCGCTAGGGAGCAGCTGGCGTTGGAGCATGAATCCTAC 
                     GAGATGGGCGAGGCTAGGTTCCGCAAGATGTTCGAGCGACAATTGAAGGCAGGGGAGG 
                     TGGCGGACAACGCTGCCGCCAAGCCCCTGATCACAACCTTGCTGCCCAAAATGATCGC 
                     GCGGATCAACGATTGGTTTGAGGAGGTTAAGGCAAAACGGGGCAAACGCCCGACCGCA 
                     TTTCAATTCCTCCAAGAAATCAAGCCTGAGGCTGTTGCCTACATCACTATCAAGACGA 
                     CACTGGCGTGTCTCACAAGCGCCGACAACACCACCGTGCAAGCCGTCGCCAGCGCCAT 
                     CGGGCGGGCAATTGAGGATGAGGCACGGTTTGGTAGGATCCGAGACCTGGAAGCGAAG 
                     CACTTCAAGAAGAACGTGGAAGAGCAGTTGAACAAACGCGTCGGCCACGTGTATAAAA 
                     AGGCTTTCATGCAGGTGGTGGAGGCCGATATGCTCAGTAAGGGGCTGCTTGGGGGGGA 
                     GGCGTGGTCATCCTGGCACAAGGAGGATAGCATTCACGTGGGGGTCCGATGTATCGAG 
                     ATGCTGATAGAGAGCACCGGAATGGTCTCCCTCCATCGCCAGAACGCTGGGGTCGTAG 
                     GGCAGGACTCCGAGACTATTGAGCTGGCCCCCGAGTATGCCGAAGCAATCGCTACACG 
                     CGCAGGTGCACTGGCTGGGATAAGCCCTATGTTTCAGCCCTGCGTAGTGCCTCCAAAG 
                     CCATGGACCGGCATCACAGGGGGTGGCTATTGGGCCAACGGTAGGCGGCCTCTGGCCC 
                     TGGTACGCACGCACAGCAAGAAGGCGCTCATGCGCTATGAAGACGTTTACATGCCCGA 
                     GGTTTACAAGGCGATCAATATCGCGCAGAACACCGCCTGGAAAATCAATAAGAAGGTG 
                     TTGGCGGTCGCAAACGTGATTACCAAGTGGAAGCATTGCCCAGTCGAGGACATACCCG 
                     CCATAGAACGCGAAGAGCTGCCGATGAAGCCGGAAGACATTGATATGAACCCCGAGGC 
                     CCTCACCGCGTGGAAAAGAGCCGCAGCCGCCGTATACAGGAAGGATAAAGCGCGCAAG 
                     TCCCGACGCATAAGCCTCGAGTTTATGCTGGAACAGGCCAACAAGTTCGCCAACCACA 
                     AAGCTATCTGGTTCCCCTACAACATGGACTGGAGAGGGAGGGTCTACGCCGTCAGCAT 
                     GTTCAATCCCCAGGGCAACGACATGACGAAGGGCCTTCTGACATTGGCAAAGGGGAAG 
                     CCTATCGGAAAGGAGGGGTACTACTGGCTCAAGATCCACGGCGCCAACTGCGCGGGAG 
                     TGGACAAGGTTCCATTTCCCGAGCGAATTAAGTTCATCGAGGAAAACCACGAAAACAT 
                     TATGGCGTGCGCTAAATCCCCCCTCGAGAACACATGGTGGGCCGAGCAAGACTCCCCG 
                     TTCTGTTTTTTGGCATTCTGCTTTGAGTACGCCGGTGTGCAGCACCATGGCCTCTCAT 
                     ACAACTGTTCCCTGCCCCTGGCCTTCGACGGAAGTTGCAGTGGGATTCAACATTTCAG 
                     CGCAATGTTGCGGGACGAGGTCGGTGGCAGGGCCGTTAACCTGCTCCCTTCCGAAACG 
                     GTGCAGGACATCTACGGAATCGTGGCAAAAAAGGTAAACGAGATCCTGCAAGCGGATG 
                     CCATCAACGGGACGGACAATGAGGTCGTTACGGTGACAGACGAAAATACTGGGGAAAT 
                     AAGCGAAAAGGTCAAGCTGGGGACCAAAGCACTCGCGGGTCAGTGGCTCGCCTACGGG 
                     GTGACACGCTCCGTCACCAAGAGAAGCGTGATGACCCTCGCGTACGGTTCAAAAGAAT 
                     TCGCTTCCGCCAGCAAGTGCTGGAGGACACCATCCAGCCGGCGATTGACTCCGGGAAG 
                     GGTCTCATGTTTACCCAGCCGAACCAGGCCGCAGGGTACATGGCCAAACTGATCTGGG 
                     AAAGCGTTAGCGTCACAGTGGTCGCCGCGGTTGAGGCGATGAATTGGCTGAAGAGCGC 
                     GGCAAAGCTCCTCGCCGCTGAGGTGAAGGACAAAAAGACCGGCGAAATCCTGCGCAAG 
                     CGCTGCGCCGTCCACTGGGTCACGCCGGATGGATTCCCCGTCTGGCAGGAGTACAAGA 
                     AGCCCATCCAAACCCGGCTCAACTTGATGTTCCTTGGCCAGTTTCGCCTGCAGCCCAC 
                     GATAAACACCAACAAAGACAGCGAGATCGACGCCCACAAGCAGGAGAGCGGCATCGCG 
                     CCCAACTTCGTGCACAGTCAGGACGGGTCCCATCTGCGGAAAACTGTTGTGTGGGCTC 
                     ACGAGAAGTACGGCATTGAGAGCTTCGCCCTGATACACGACAGCTTCGGGACCATACC 
                     AGCGGACGCAGCGAACCTGTTCAAAGCCGTGCGGGAAACAATGGTCGACACCTACGAA 
                     AGCTGCGACGTACTGGCAGACTTCTATGACCAATTCGCCGACCAGCTTCACGAGTCAC 
                     AGCTCGACAAGATGCCCGCTCTGCCCGCGAAAGGCAACCTGAATTTGCGCGACAT"
                     /label=nonstandard type: Editing History Replacement
     CDS             945..5192
                     /codon_start=1
                     /standard_name="EGFP/BLM"
                     /label=EGFP/BLM
                     /translation="AAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNN
                     VSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSK
                     SLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSE
                     TSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEE
                     QKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHS
                     TEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDL
                     LSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNEL
                     LQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLP
                     SNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESS
                     YFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAAS
                     KSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLAS
                     RNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGK
                     SLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLS
                     KKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKR
                     MNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPK
                     KVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQ
                     QKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCL
                     LFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENG
                     FNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGR
                     FTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQA
                     IAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVC
                     KSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKY
                     SEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQR
                     SKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPK
                     PINRPFLKPSYAFS"
     misc_feature    945..966
                     /standard_name="BLM_1F"
                     /label=BLM_1F
     misc_feature    1371..1373
                     /standard_name="S144"
                     /label=S144
     misc_feature    1577..1596
                     /standard_name="BLM_3F"
                     /label=BLM_3F
     misc_feature    2309..2328
                     /standard_name="BLM_4F"
                     /label=BLM_4F
     misc_feature    2582..2601
                     /standard_name="BLM_2F"
                     /label=BLM_2F
     misc_feature    2955..2957
                     /standard_name="Q672"
                     /label=Q672
     misc_feature    3024..3026
                     /standard_name="Walker A"
                     /label=Walker A
     misc_feature    3331..3350
                     /standard_name="BLM_5F"
                     /label=BLM_5F
     misc_feature    4067..4086
                     /standard_name="BLM_6F"
                     /label=BLM_6F
     misc_feature    4340..4359
                     /standard_name="FBlm3398"
                     /label=FBlm3398
     misc_feature    4386..4406
                     /standard_name="SacI-FBlm3444"
                     /label=SacI-FBlm3444
     misc_feature    4575..4817
                     /label=HRDC
                     /note="HRDC"
     misc_feature    4838..4857
                     /standard_name="BLM_7F"
                     /label=BLM_7F
     misc_feature    4941..4988
                     /label=NLS
                     /note="NLS"
     misc_feature    5032..5054
                     /standard_name="BLM_9F"
                     /label=BLM_9F
     misc_feature    5056..5074
                     /standard_name="BLM_8F"
                     /label=BLM_8F
     primer_bind     5158..5217
                     /standard_name="AID_BLM_UGI_BB_Fwd"
                     /label=AID_BLM_UGI_BB_Fwd
     primer_bind     complement(5165..5192)
                     /standard_name="BLM_rev"
                     /label=BLM_rev
     misc_feature    5193..5453
                     /standard_name="Linker+UGI (concatenated sequence 2)"
                     /label=nonstandard type: Concatenated sequence
     primer_bind     5193..5217
                     /standard_name="with_ugi_primer_dimer"
                     /label=with_ugi_primer_dimer
     CDS             5205..5453
                     /standard_name="UGI"
                     /label=UGI
     primer_bind     5454..5474
                     /standard_name="no_ugi"
                     /label=no_ugi
     CDS             5466..5486
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5495..5512
                     /codon_start=1
                     /standard_name="6xHis"
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     5513..5534
                     /standard_name="5,565 F"
                     /label=5,565 F
     primer_bind     5513..5534
                     /standard_name="Express Fwd"
                     /label=Express Fwd
     primer_bind     complement(5535..5552)
                     /standard_name="BGH-rev"
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.
                     Also called BGH reverse"
     polyA_signal    5541..5765
                     /label=bovine growth hormone polyadenylation signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="/standard_name=bGH poly(A) signal"
     misc_feature    complement(5607..5626)
                     /direction=LEFT
                     /standard_name="5,678 R"
                     /label=nonstandard type: primer_bind_reverse
     primer_bind     complement(5797..5811)
                     /standard_name="pBluescriptKS"
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5836..5852)
                     /standard_name="M13 rev"
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5836..5852)
                     /standard_name="M13 Reverse"
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5849..5871)
                     /standard_name="M13/pUC Reverse"
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5860..5876
                     /standard_name="lac operator"
                     /label=lac operator binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5884..5914)
                     /standard_name="lac promoter"
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5929..5950
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
     primer_bind     complement(6067..6084)
                     /standard_name="L4440"
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6238..6826)
                     /direction=LEFT
     primer_bind     complement(6318..6337)
                     /standard_name="pBR322ori-F"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6997..7857)
                     /codon_start=1
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7620..7639
                     /standard_name="Amp-R"
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7858..7962)
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
     primer_bind     complement(8041..8060)
                     /standard_name="pRS-marker"
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        join(8232..8480,1..131)
                     /standard_name="CMV enhancer"
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgaga gccgccacca tgagttctga ggtggagttt tcccacgagt
      421 actggatgag acatgccctg accctggcca agagggcacg ggatgagagg gaggtgcctg
      481 tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac agagccatcg
      541 gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc ggcctggtca
      601 tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct tgcgtgatgt
      661 gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg aggaactcaa
      721 aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg aatcaccgcg
      781 tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc gatttctatc
      841 ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc aacagcggca
      901 gcgagactcc cgggacctca gagtccgcca cacccgaaag tatggctgct gttcctcaaa
      961 ataatctaca ggagcaacta gaacgtcact cagccagaac acttaataat aaattaagtc
     1021 tttcaaaacc aaaattttca ggtttcactt ttaaaaagaa aacatcttca gataacaatg
     1081 tatctgtaac taatgtgtca gtagcaaaaa cacctgtatt aagaaataaa gatgttaatg
     1141 ttaccgaaga cttttccttc agtgaacctc tacccaacac cacaaatcag caaagggtca
     1201 aggacttctt taaaaatgct ccagcaggac aggaaacaca gagaggtgga tcaaaatcat
     1261 tattgccaga tttcttgcag actccgaagg aagttgtatg cactacccaa aacacaccaa
     1321 ctgtaaagaa atcccgggat actgctctca agaaattaga atttagttct tcaccagatt
     1381 ctttaagtac catcaatgat tgggatgata tggatgactt tgatacttct gagacttcaa
     1441 aatcatttgt tacaccaccc caaagtcact ttgtaagagt aagcactgct cagaaatcaa
     1501 aaaagggtaa gagaaacttt tttaaagcac agctttatac aacaaacaca gtaaagactg
     1561 acttgcctcc accctcctct gaaagcgagc aaatagattt gactgaggaa cagaaggatg
     1621 actcagaatg gttaagcagc gatgtgattt gcatcgatga tggccccatt gctgaagtgc
     1681 atataaatga agatgctcag gaaagtgact ctctgaaaac tcatttggaa gatgaaagag
     1741 ataatagcga aaagaagaag aatttggaag aagctgaatt acattcaact gagaaagttc
     1801 catgtattga atttgatgat gatgattatg atacggattt tgttccacct tctccagaag
     1861 aaattatttc tgcttcttct tcctcttcaa aatgccttag tacgttaaag gaccttgaca
     1921 cctctgacag aaaagaggat gttcttagca catcaaaaga tcttttgtca aaacctgaga
     1981 aaatgagtat gcaggagctg aatccagaaa ccagcacaga ctgtgacgct agacagataa
     2041 gtttacagca gcagcttatt catgtgatgg agcacatctg taaattaatt gatactattc
     2101 ctgatgataa actgaaactt ttggattgtg ggaacgaact gcttcagcag cggaacataa
     2161 gaaggaaact tctaacggaa gtagatttta ataaaagtga tgccagtctt cttggctcat
     2221 tgtggagata caggcctgat tcacttgatg gccctatgga gggtgattcc tgccctacag
     2281 ggaattctat gaaggagtta aatttttcac accttccctc aaattctgtt tctcctgggg
     2341 actgtttact gactaccacc ctaggaaaga caggattctc tgccaccagg aagaatcttt
     2401 ttgaaaggcc tttattcaat acccatttac agaagtcctt tgtaagtagc aactgggctg
     2461 aaacaccaag actaggaaaa aaaaatgaaa gctcttattt cccaggaaat gttctcacaa
     2521 gcactgctgt gaaagatcag aataaacata ctgcttcaat aaatgactta gaaagagaaa
     2581 cccaaccttc ctatgatatt gataattttg acatagatga ctttgatgat gatgatgact
     2641 gggaagacat aatgcataat ttagcagcca gcaaatcttc cacagctgcc tatcaaccca
     2701 tcaaggaagg tcggccaatt aaatcagtat cagaaagact ttcctcagcc aagacagact
     2761 gtcttccagt gtcatctact gctcaaaata taaacttctc agagtcaatt cagaattata
     2821 ctgacaagtc agcacaaaat ttagcatcca gaaatctgaa acatgagcgt ttccaaagtc
     2881 ttagttttcc tcatacaaag gaaatgatga agatttttca taaaaaattt ggcctgcata
     2941 attttagaac taatcagcta gaggcgatca atgctgcact gcttggtgaa gactgtttta
     3001 tcctgatgcc gactggaggt ggtaagagtt tgtgttacca gctccctgcc tgtgtttctc
     3061 ctggggtcac tgttgtcatt tctcccttga gatcacttat cgtagatcaa gtccaaaagc
     3121 tgacttcctt ggatattcca gctacatatc tgacaggtga taagactgac tcagaagcta
     3181 caaatattta cctccagtta tcaaaaaaag acccaatcat aaaactccta tatgtcactc
     3241 cagaaaagat ctgtgcaagt aacagactca tttctactct ggagaatctc tatgagagga
     3301 agctcttggc acgttttgtt attgatgaag cacattgtgt cagtcagtgg ggacatgatt
     3361 ttcgtcaaga ttacaaaaga atgaatatgc ttcgccagaa gtttccttct gttccggtga
     3421 tggctcttac ggccacagct aatcccaggg tacagaagga catcctgact cagctgaaga
     3481 ttctcagacc tcaggtgttt agcatgagct ttaacagaca taatctgaaa tactatgtat
     3541 taccgaaaaa gcctaaaaag gtggcatttg attgcctaga atggatcaga aagcaccacc
     3601 catatgattc agggataatt tactgcctct ccaggcgaga atgtgacacc atggctgaca
     3661 cgttacagag agatgggctc gctgctcttg cttaccatgc tggcctcagt gattctgcca
     3721 gagatgaagt gcagcagaag tggattaatc aggatggctg tcaggttatc tgtgctacaa
     3781 ttgcatttgg aatggggatt gacaaaccgg acgtgcgatt tgtgattcat gcatctctcc
     3841 ctaaatctgt ggagggttac taccaagaat ctggcagagc tggaagagat ggggaaatat
     3901 ctcactgcct gcttttctat acctatcatg atgtgaccag actgaaaaga cttataatga
     3961 tggaaaaaga tggaaaccat catacaagag aaactcactt caataatttg tatagcatgg
     4021 tacattactg tgaaaatata acagaatgca ggagaataca gcttttggcc tactttggtg
     4081 aaaatggatt taatcctgat ttttgtaaga aacacccaga tgtttcttgt gataattgct
     4141 gtaaaacaaa ggattataaa acaagagatg tgactgacga tgtgaaaagt attgtaagat
     4201 ttgttcaaga acatagttca tcacaaggaa tgagaaatat aaaacatgta ggtccttctg
     4261 gaagatttac tatgaatatg ctggtcgaca ttttcttggg gagtaagagt gcaaaaatcc
     4321 agtcaggtat atttggaaaa ggatctgctt attcacgaca caatgccgaa agacttttta
     4381 aaaagctgat acttgacaag attttggatg aagacttata tatcaatgcc aatgaccagg
     4441 cgatcgctta tgtgatgctc ggaaataaag cacaaactgt actaaatggc aatttaaagg
     4501 tagactttat ggaaacagaa aattccagca gtgtgaaaaa acaaaaagcg ttagtagcaa
     4561 aagtgtctca gagggaagag atggttaaaa aatgtcttgg agaacttaca gaagtctgca
     4621 aatctctggg gaaagttttt ggtgtccatt acttcaatat ttttaatacc gtcactctca
     4681 agaagcttgc agaatcttta tcttctgatc ctgaggtttt gcttcaaatt gatggtgtta
     4741 ctgaagacaa actggaaaaa tatggtgcgg aagtgatttc agtattacag aaatactctg
     4801 aatggacatc gccagctgaa gacagttccc cagggataag cctgtccagc agcagaggcc
     4861 ccggaagaag tgccgctgag gagcttgacg aggaaatacc cgtatcttcc cactactttg
     4921 caagtaaaac cagaaatgaa aggaagagga aaaagatgcc agcctcccaa aggtctaaga
     4981 ggagaaaaac tgcttccagt ggttccaagg caaagggggg gtctgccaca tgtagaaaga
     5041 tatcttccaa aacgaaatcc tccagcatca ttggatccag ttcagcctca catacttctc
     5101 aagcgacatc aggagccaat agcaaattgg ggattatggc tccaccgaag cctataaata
     5161 gaccgtttct taagccttca tatgcattct catctggtgg ttctactaat ctgtcagata
     5221 ttattgaaaa ggagaccggt aagcaactgg ttatccagga atccatcctc atgctcccag
     5281 aggaggtgga agaagtcatt gggaacaagc cggaaagcga tatactcgtg cacaccgcct
     5341 acgacgagag caccgacgag aatgtcatgc ttctgactag cgacgcccct gaatacaagc
     5401 cttgggctct ggtcatacag gatagcaacg gtgagaacaa gattaagatg ctctctggtg
     5461 gttctcccaa gaagaagagg aaagtctaac cggtcatcat caccatcacc attgagttta
     5521 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5581 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5641 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5701 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     5761 tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag
     5821 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5881 acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta
     5941 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6001 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6061 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6121 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6181 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6241 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6301 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6361 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6421 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6481 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6541 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6601 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6661 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6721 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6781 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6841 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6901 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6961 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7021 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7081 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7141 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7201 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7261 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7321 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7381 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7441 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7501 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7561 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7621 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7681 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7741 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7801 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7861 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7921 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7981 gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt cgactctcag
     8041 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     8101 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     8161 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     8221 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     8281 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     8341 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     8401 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     8461 ggcagtacat caagtgtatc
//
