LOCUS       Exported                8829 bp ds-DNA     circular SYN 21-JAN-2016
DEFINITION  Vector for fusing ECFP to the N-terminus of a partner protein.
ACCESSION   .
VERSION     .
KEYWORDS    pECFP-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Clontech
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 15, 2024 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     .
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             613..1329
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1331..1350
                     /label=Vectorpart of FP Gibson
     CDS             1351..5445
                     /codon_start=1
                     /label=NSD2 Fulllenght
                     /translation="MEFSIKQSPLSVQSVVKCIKMKQAPEILGSANGKTPSCEVNRECS
                     VFLSKAQLSSSLQEGVMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQE
                     MKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFESSICGDSAADVSQSEENGQKPEN
                     KARRNRKRSIKYDSLLEQGLVEAALVSKISSPSDKKIPAKKESCPNTGRDKDHLLKYNV
                     GDLVWSKVSGYPWWPCMVSADPLLHSYTKLKGQKKSARQYHVQFFGDAPERAWIFEKSL
                     VAFEGEGQFEKLCQESAKQAPTKAEKIKLLKPISGKLRAQWEMGIVQAEEAASMSVEER
                     KAKFTFLYVGDQLHLNPQVAKEAGIAAESLGEMAESSGVSEEAAENPKSVREECIPMKR
                     RRRAKLCSSAETLESHPDIGKSTPQKTAEADPRRGVGSPPGRKKTTVSMPRSRKGDAAS
                     QFLVFCQKHRDEVVAEHPDASGEEIEELLRSQWSLLSEKQRARYNTKFALVAPVQAEED
                     SGNVNGKKRNHTKRIQDPTEDAEAEDTPRKRLRTDKHSLRKRDTITDKTARTSSYKAME
                     AASSLKSQAATKNLSDACKPLKKRNRASTAASSALGFSKSSSPSASLTENEVSDSPGDE
                     PSESPYESADETQTEVSVSSKKSERGVTAKKEYVCQLCEKPGSLLLCEGPCCGAFHLAC
                     LGLSRRPEGRFTCSECASGIHSCFVCKESKTDVKRCVVTQCGKFYHEACVKKYPLTVFE
                     SRGFRCPLHSCVSCHASNPSNPRPSKGKMMRCVRCPVAYHSGDACLAAGCSVIASNSII
                     CTAHFTARKGKRHHAHVNVSWCFVCSKGGSLLCCESCPAAFHPDCLNIEMPDGSWFCND
                     CRAGKKLHFQDIIWVKLGNYRWWPAEVCHPKNVPPNIQKMKHEIGEFPVFFFGSKDYYW
                     THQARVFPYMEGDRGSRYQGVRGIGRVFKNALQEAEARFREIKLQREARETQESERKPP
                     PYKHIKVNKPYGKVQIYTADISEIPKCNCKPTDENPCGFDSECLNRMLMFECHPQVCPA
                     GEFCQNQCFTKRQYPETKIIKTDGKGWGLVAKRDIRKGEFVNEYVGELIDEEECMARIK
                     HAHENDITHFYMLTIDKDRIIDAGPKGNYSRFMNHSCQPNCETLKWTVNGDTRVGLFAV
                     CDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSGFLGDRPKTSTTLSSEEKGKKTKKKT
                     RRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCTKAYHLSCLGLGKRPFGKWECPWHH
                     CDVCGKPSTSFCHLCPNSFCKEHQDGTAFSCTPDGRSYCCEHDLGAASVRSTKTEKPPP
                     EPGKPKGKRRRRRGWRRVTEGK"
     misc_feature    1351..1373
                     /label=FP for Gibson
     CDS             3262..5070
                     /codon_start=1
                     /label=PHD1 - pSET
                     /translation="ESPYESADETQTEVSVSSKKSERGVTAKKEYVCQLCEKPGSLLLC
                     EGPCCGAFHLACLGLSRRPEGRFTCSECASGIHSCFVCKESKTDVKRCVVTQCGKFYHE
                     ACVKKYPLTVFESRGFRCPLHSCVSCHASNPSNPRPSKGKMMRCVRCPVAYHSGDACLA
                     AGCSVIASNSIICTAHFTARKGKRHHAHVNVSWCFVCSKGGSLLCCESCPAAFHPDCLN
                     IEMPDGSWFCNDCRAGKKLHFQDIIWVKLGNYRWWPAEVCHPKNVPPNIQKMKHEIGEF
                     PVFFFGSKDYYWTHQARVFPYMEGDRGSRYQGVRGIGRVFKNALQEAEARFREIKLQRE
                     ARETQESERKPPPYKHIKVNKPYGKVQIYTADISEIPKCNCKPTDENPCGFDSECLNRM
                     LMFECHPQVCPAGEFCQNQCFTKRQYPETKIIKTDGKGWGLVAKRDIRKGEFVNEYVGE
                     LIDEEECMARIKHAHENDITHFYMLTIDKDRIIDAGPKGNYSRFMNHSCQPNCETLKWT
                     VNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSGFLGDRPKTSTTLS
                     SEEKGKKTKKKTRRRRAKGEGKRQSED"
     CDS             3358..3480
                     /codon_start=1
                     /label=PHD1
                     /translation="CQLCEKPGSLLLCEGPCCGAFHLACLGLSRRPEGRFTCSEC"
     CDS             3499..3636
                     /codon_start=1
                     /label=PHD2
                     /translation="CFVCKESKTDVKRCVVTQCGKFYHEACVKKYPLTVFESRGFRCPL
                     H"
     CDS             3640..3798
                     /codon_start=1
                     /label=PHD3
                     /translation="CVSCHASNPSNPRPSKGKMMRCVRCPVAYHSGDACLAAGCSVIAS
                     NSIICTAH"
     CDS             3850..3966
                     /codon_start=1
                     /label=PHD4
                     /translation="CFVCSKGGSLLCCESCPAAFHPDCLNIEMPDGSWFCNDC"
     CDS             3982..4170
                     /codon_start=1
                     /label=PWWP 2
                     /translation="LHFQDIIWVKLGNYRWWPAEVCHPKNVPPNIQKMKHEIGEFPVFF
                     FGSKDYYWTHQARVFPYM"
     misc_feature    4645..4647
                     /label=E1099
     misc_feature    4723..4725
                     /label=D1125
     misc_feature    5426..5445
                     /label=RP for Gibson
     misc_feature    5446..5473
                     /label=Vectorpart of RP Gibson
     misc_feature    5446..5448
                     /label=Synthetic STOP CODON
     misc_feature    5502..5512
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     polyA_signal    5617..5738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5745..6200)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6227..6331
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6333..6690
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6541..6676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6725..7519
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    7751..7798
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      8127..8715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct atggaattta gcatcaagca gagtcccctt
     1381 tctgttcaga gtgttgtaaa gtgcataaag atgaagcagg caccagaaat cctcggcagt
     1441 gccaacggga agactccgag ctgcgaggtg aaccgcgagt gttctgtgtt cctcagcaaa
     1501 gcccagctct ccagtagcct gcaggagggg gtcatgcaga agtttaacgg ccacgacgcc
     1561 ctgcccttta ttccagccga caagctgaaa gatcttactt cccgggtgtt taatggagaa
     1621 cccggcgcac acgatgccaa actgcgtttt gagtcccagg aaatgaaagg gattgggaca
     1681 ccccctaaca ctacccctat caaaaatggc tctccagaaa ttaagctgaa aatcaccaaa
     1741 acatacatga atgggaagcc tctctttgaa tcttccattt gtggtgacag tgctgctgat
     1801 gtgtctcagt cagaagaaaa tggacaaaaa ccagaaaaca aggcgagaag gaacaggaag
     1861 aggagcataa aatatgactc cttgctggag cagggccttg tcgaagcagc tcttgtgtct
     1921 aagatctcaa gtccttcaga taaaaagatt ccagctaaga aagagtcttg tccaaacact
     1981 ggaagagaca aagaccacct gttgaaatac aacgttggtg atttggtgtg gtccaaagtg
     2041 tcgggttacc cttggtggcc ttgcatggtt tctgcagatc cactccttca cagctatacc
     2101 aaacttaaag gtcagaaaaa gagtgcacgc cagtatcacg tacagttctt tggtgacgcc
     2161 ccagaaagag cttggatatt tgagaagagc ctcgtagctt ttgaaggaga aggacagttt
     2221 gaaaaattat gccaggaaag tgccaagcag gcacccacga aagctgagaa aattaagcta
     2281 ttgaaaccaa tttcagggaa attgagggcc cagtgggaaa tgggcattgt tcaagcagaa
     2341 gaagctgcaa gcatgtcagt ggaggagcgg aaagccaagt tcacctttct ctatgtgggg
     2401 gaccagcttc atctcaaccc tcaagtagcc aaggaggctg gcattgctgc agagtctttg
     2461 ggagaaatgg cagaatcctc aggagtcagt gaagaagctg ctgaaaaccc caagtctgtg
     2521 agagaagagt gcattcccat gaagagaagg cggagggcca aactgtgtag ctctgcagag
     2581 accctggaga gtcaccccga catagggaag agtactcctc aaaagacggc agaggctgac
     2641 cccagaagag gagtagggtc tcctcctggg aggaagaaga ccacagtctc catgccacga
     2701 agcaggaagg gagatgcagc atcccagttt ttggtcttct gtcaaaaaca cagggatgag
     2761 gtggtagctg agcacccaga tgcttcaggt gaggagattg aagagctgct caggtcacag
     2821 tggagtctgc tgagtgagaa gcagagagca cgctacaaca ccaagtttgc cctggtggcc
     2881 cctgtccagg ctgaagaaga ctctggtaat gtaaatggga aaaaaagaaa ccacacaaag
     2941 aggatacagg accctacaga agatgctgaa gctgaggaca cacccaggaa aagactcagg
     3001 acggacaagc acagtcttcg gaagagagac acaatcactg acaaaacggc cagaacaagc
     3061 tcttacaagg ccatggaggc agcctcctcg ctcaagagcc aggcagcaac gaaaaatctg
     3121 tctgatgcat gtaaaccact gaagaagcga aatcgggctt ccacggcagc atcttcagct
     3181 cttgggttta gcaaaagttc atctccttct gcatccttaa ctgagaatga ggtctcggac
     3241 agcccgggag acgagccctc ggagtcccca tacgaaagtg cagacgaaac acaaactgaa
     3301 gtatctgtct catccaaaaa gtctgagcga ggagtgactg ccaaaaagga gtatgtgtgc
     3361 cagctgtgtg agaagccggg cagcctcctg ctctgtgaag gaccctgctg cggagctttc
     3421 cacctcgcct gccttgggct ttcccggagg ccagaaggga ggttcacctg cagcgagtgt
     3481 gcctcaggga ttcactcatg tttcgtgtgt aaagagagca agacagatgt taagcgctgt
     3541 gtggtaactc agtgtggaaa attttaccat gaggcttgtg tgaaaaaata ccctctgact
     3601 gtatttgaga gccgaggttt ccgctgcccc ctccacagct gtgtgagctg ccatgcttcc
     3661 aacccttcaa acccaaggcc gtcaaaaggt aaaatgatgc ggtgtgtccg ctgccccgtt
     3721 gcctatcaca gcggggatgc ttgtctggca gcaggatgct cagtgatcgc ctccaacagc
     3781 atcatctgca ctgcccactt cactgctcgg aaggggaagc gacaccacgc ccacgtcaac
     3841 gtgagctggt gcttcgtgtg ctccaaaggg gggagccttc tgtgctgtga gtcctgccca
     3901 gcggccttcc accctgactg cctgaacatc gagatgcctg acggcagctg gttctgcaat
     3961 gactgcaggg ctgggaagaa gctgcacttc caggatatca tttgggtgaa acttgggaac
     4021 tacagatggt ggccggcaga agtttgccat cccaaaaatg ttcccccaaa tattcagaaa
     4081 atgaagcacg agattggaga attccctgtg tttttctttg ggtctaaaga ttattactgg
     4141 acgcatcagg cgcgagtgtt cccgtacatg gagggggacc ggggcagccg ctaccagggg
     4201 gtcagaggga tcggaagagt cttcaaaaac gcactgcaag aagctgaagc tcgttttcgt
     4261 gaaattaagc ttcagaggga agcccgagaa acacaggaga gcgagcgcaa gcccccacca
     4321 tacaagcaca tcaaggtgaa taagccttac gggaaagtcc agatctacac agcggatatt
     4381 tcagaaatcc ctaagtgcaa ctgcaagccc acagatgaga atccttgtgg ctttgattcg
     4441 gagtgtctga acaggatgct gatgtttgag tgccacccgc aggtgtgtcc cgcgggcgag
     4501 ttctgccaga accagtgctt caccaagcgc cagtacccag agaccaagat catcaagaca
     4561 gatggcaaag ggtggggcct ggtcgccaag agggacatca gaaagggaga atttgttaac
     4621 gagtacgttg gggagctgat cgacgaggag gagtgcatgg cgagaatcaa gcacgcacac
     4681 gagaacgaca tcacccactt ctacatgctc actatagaca aggaccgtat aatagacgct
     4741 ggccccaaag gaaactactc tcgatttatg aatcacagct gccagcccaa ctgtgagacc
     4801 ctcaagtgga cagtgaatgg ggacactcgt gtgggcctgt ttgccgtctg tgacattcct
     4861 gcagggacgg agctgacttt taactacaac ctcgattgtc tgggcaatga aaaaacggtc
     4921 tgccggtgtg gagcctccaa ttgcagtgga ttcctcgggg atagaccaaa gacctcgacg
     4981 accctttcat cagaggaaaa gggcaaaaag accaagaaga aaacgaggcg gcgcagagca
     5041 aaaggggaag ggaagaggca gtcagaggac gagtgcttcc gctgcggtga tggcgggcag
     5101 ctggtgctgt gtgaccgcaa gttctgcacc aaggcctacc acctgtcctg cctgggcctt
     5161 ggcaagcggc ccttcgggaa gtgggaatgt ccttggcatc attgtgacgt gtgtggcaaa
     5221 ccttcgactt cattttgcca cctctgcccc aattcgttct gtaaggagca ccaggacggg
     5281 acagccttca gctgcacccc ggacgggcgg tcctactgct gtgagcatga cttaggggcg
     5341 gcatcggtca gaagcaccaa gactgagaag ccccccccag agccagggaa gccgaagggg
     5401 aagaggcggc ggcggagggg ctggcggaga gtcacagagg gcaaataaca agcttcgaat
     5461 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     5521 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     5581 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     5641 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5701 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     5761 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     5821 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     5881 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5941 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     6001 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     6061 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     6121 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     6181 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6301 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     6361 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6421 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6481 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6541 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6601 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     6661 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     6721 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     6781 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6841 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6901 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     6961 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7021 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7081 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7141 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7201 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     7261 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     7321 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     7381 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     7441 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     7501 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     7561 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     7621 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     7681 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     7741 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     7801 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     7861 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     7921 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     7981 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8041 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8101 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8161 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8221 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     8281 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8341 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8401 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8461 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8521 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8581 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8641 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8701 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8761 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8821 gccatgcat
//
