LOCUS       pDN-Cherry-sgRNA        6683 bp    DNA     circular SYN 24-Oct-2022
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
FEATURES             Location/Qualifiers
     misc_feature    1..28
                     /label="pJR962-Psmyc-F"
                     /note="; /ApEinfo_revcolor=""#b1ff67"";
                     /ApEinfo_fwdcolor=""#b1ff67"""
     regulatory      174..237
                     /regulatory_class="promoter"
                     /label="Psmyc"
                     /mv_symbol_type=SingleArrow
                     /mv_fill_color="1.000,0.400,0.400,1.000"
                     /mv_pen_color="0.500,0.200,0.200,1.000"
     misc_feature    complement(324..349)
                     /label="pJR962-Psmyc-F"
                     /note="; /ApEinfo_revcolor=""#b1ff67"";
                     /ApEinfo_fwdcolor=""#b1ff67"""
     CDS             350..1060
                     /label="mCherry"
                     /note=""
                     /note="; /ApEinfo_revcolor=""#ff9ccd"";
                     /ApEinfo_fwdcolor=""#ff9ccd"""
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="1.000,0.400,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_fontsize=12.000000
                     /mv_show_residues_if_room=ShowAsGraphics
                     /mv_level=0
     misc_feature    complement(719..738)
                     /label="pDN-Cherry-Psmyc-Seq"
                     /note="; /ApEinfo_revcolor=""#d6b295"";
                     /ApEinfo_fwdcolor=""#d6b295"""
     rep_origin      1129..3288
                     /label="oriM"
                     /note="/bound_moiety=oriM"
                     /mv_symbol_type=ThinHollowBox
                     /mv_fill=1
                     /mv_fill_color="0.664,0.664,0.664,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="oriM"
                     /mv_fontsize=11.000000
                     /mv_label_position=AboveLeft
                     /mv_level=0
     CDS             1863..2789
                     /label="repA"
                     /mv_fill_color="0.837,0.837,0.837,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_show_residues_if_room=ShowAsGraphics
                     /mv_level=0
     CDS             2786..3133
                     /label="repB"
                     /mv_fill_color="0.837,0.837,0.837,1.000"
                     /mv_level=0
     misc_feature    3202..3221
                     /label="pDN-Cherry-sgRNA-Seq"
                     /note="; /ApEinfo_revcolor=""#ff9ccd"";
                     /ApEinfo_fwdcolor=""#ff9ccd"""
     misc_feature    3282..3306
                     /label="pJR962-sgRNA-F"
                     /note="; /ApEinfo_revcolor=""#c7b0e3"";
                     /ApEinfo_fwdcolor=""#c7b0e3"""
     misc_feature    3283..3789
                     /label="sgRNA from pJR962"
                     /note="; /ApEinfo_revcolor=""#ff9ccd"";
                     /ApEinfo_fwdcolor=""#ff9ccd"""
     misc_feature    3307..3359
                     /label="rnBT1"
                     /note="; /ApEinfo_revcolor=""#faac61"";
                     /ApEinfo_fwdcolor=""#faac61"""
     misc_feature    3360..3397
                     /label="TsynB"
                     /note="; /ApEinfo_revcolor=""#75c6a9"";
                     /ApEinfo_fwdcolor=""#75c6a9"""
     misc_feature    3399..3421
                     /label="Golden-F1"
                     /note="; /ApEinfo_revcolor=""#f8d3a9"";
                     /ApEinfo_fwdcolor=""#f8d3a9"""
     regulatory      3404..3474
                     /regulatory_class="promoter"
                     /label="P17 derivative"
                     /note=""
                     /note="; /ApEinfo_revcolor=""#ff9ccd"";
                     /ApEinfo_fwdcolor=""#ff9ccd"""
                     /mv_symbol_type=SingleArrow
                     /mv_fill_color="1.000,0.400,0.400,1.000"
                     /mv_pen_color="0.500,0.200,0.200,1.000"
     protein_bind    3445..3461
                     /bound_moiety="TetR"
                     /label="tetO"
                     /note=""
                     /note=""
                     /note="; /ApEinfo_revcolor=""#85dae9"";
                     /ApEinfo_fwdcolor=""#85dae9"""
                     /mv_symbol_type=ThinHollowBox
                     /mv_fill_color="0.000,0.667,0.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_visible=1
                     /mv_level=0
     misc_feature    3474
                     /label="TSS"
                     /note="; /ApEinfo_revcolor=""#d59687"";
                     /ApEinfo_fwdcolor=""#d59687"""
     misc_feature    3475..3480
                     /label="BsmBI"
                     /note="; /ApEinfo_revcolor=""#faac61"";
                     /ApEinfo_fwdcolor=""#faac61"""
     misc_feature    3488..3493
                     /label="BsmBI"
                     /note="; /ApEinfo_revcolor=""#c7b0e3"";
                     /ApEinfo_fwdcolor=""#c7b0e3"""
     misc_feature    3495..3574
                     /label="Sth1 sgRNA scaffold"
                     /note=""
                     /note="; /ApEinfo_revcolor=""#ff9ccd"";
                     /ApEinfo_fwdcolor=""#ff9ccd"""
                     /mv_symbol_type=ThinHollowArrow
                     /mv_fill_color="1.000,1.000,0.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_visible=1
                     /mv_level=0
     regulatory      3545..3571
                     /regulatory_class="terminator"
                     /label="Sth1 tracrRNA term"
                     /note=""
                     /note=""
                     /note=""
                     /note="; /ApEinfo_revcolor=""#c7b0e3"";
                     /ApEinfo_fwdcolor=""#c7b0e3"""
                     /mv_symbol_type=HollowCircle
                     /mv_fill_color="1.000,0.400,0.400,1.000"
                     /mv_pen_color="0.500,0.200,0.200,1.000"
                     /mv_visible=0 
                     /mv_level=1
     misc_feature    3587..3618
                     /label="T4_32 terminator"
                     /note="; /ApEinfo_revcolor=""#ffef86"";
                     /ApEinfo_fwdcolor=""#ffef86"""
     misc_feature    complement(3601..3623)
                     /label="Golden-R1"
                     /note="; /ApEinfo_revcolor=""#85dae9"";
                     /ApEinfo_fwdcolor=""#85dae9"""
     misc_feature    3621..3654
                     /label="SapI-Golden Gate handle"
                     /note="; /ApEinfo_revcolor=""#85dae9"";
                     /ApEinfo_fwdcolor=""#85dae9"""
     misc_feature    complement(3632..3656)
                     /label="Primer-1834"
                     /note="; /ApEinfo_revcolor=""#85dae9"";
                     /ApEinfo_fwdcolor=""#85dae9"""
     misc_feature    3661..3698
                     /label="TsynA"
                     /note="; /ApEinfo_revcolor=""#75c6a9"";
                     /ApEinfo_fwdcolor=""#75c6a9"""
     misc_feature    complement(3767..3792)
                     /label="pJR962-sgRNA-R"
                     /note="; /ApEinfo_revcolor=""#faac61"";
                     /ApEinfo_fwdcolor=""#faac61"""
     misc_feature    complement(3793..3812)
                     /label="Golden-Seq"
                     /note="; /ApEinfo_revcolor=""#75c6a9"";
                     /ApEinfo_fwdcolor=""#75c6a9"""
     CDS             complement(4099..5646)
                     /label="HygR"
                     /note=""
                     /note="; /ApEinfo_revcolor=""#d6b295"";
                     /ApEinfo_fwdcolor=""#d6b295"""
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="0.000,0.600,1.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_fontsize=12.000000
                     /mv_show_residues_if_room=ShowAsGraphics
                     /mv_level=0
     rep_origin      5654..6242
                     /label="oriE"
                     /mv_symbol_type=ThinHollowBox
                     /mv_fill=1
                     /mv_fill_color="1.000,1.000,1.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_fontsize=11.000000
                     /mv_level=0
BASE COUNT  1350 A  2012 C  2093 G  1228 T  0 OTHER
ORIGIN
        1 TCTAGAAGAA ATATTGGATC GTCGGCACCG TCACGGCCGT GGGAGGCGGC ACGATCCGCG
       61 ACGTGATGAT CGGCCGCATC CCCACGGTGC TGCGCAGTGA GCTCTACGCC ATCCCGGCGT
      121 TGATCTGTGC GTTCGCACGC ACAGGCCCGG TGTGAGAAGG GTCTCTGACG AGCGGGAGAA
      181 CCCACCCGGG GTGGGCGAGT TTGTCCTGCG TGTGCTCGGT CGAGTAGGCT CTGGGAGTAC
      241 CCGTGTGTAC GACCAGCACG GCATACATCA TTTCGACGCC GAGAGATTCG CCGCCCGAAA
      301 TGAGCACGAT CCGCATGGTA CCATCAGGAG GAATCACTTC GCAGGATCCA TGGTCTCGAA
      361 GGGCGAGGAG GACAACATGG CGATCATCAA GGAGTTCATG CGCTTCAAGG TCCACATGGA
      421 GGGCTCGGTC AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCGTACGA
      481 GGGCACCCAG ACCGCCAAGC TGAAGGTCAC CAAGGGCGGC CCGCTGCCGT TCGCCTGGGA
      541 CATCCTGTCG CCGCAGTTCA TGTACGGCAG CAAGGCCTAC GTCAAGCACC CGGCCGACAT
      601 CCCGGACTAC CTGAAGCTGT CGTTCCCGGA GGGCTTCAAG TGGGAGCGCG TCATGAACTT
      661 CGAGGACGGC GGCGTCGTCA CCGTCACCCA GGACTCGTCG CTGCAGGACG GCGAGTTCAT
      721 CTACAAGGTC AAGCTGCGGG GCACCAACTT CCCGTCGGAC GGCCCGGTCA TGCAGAAGAA
      781 GACCATGGGC TGGGAGGCCT CGTCGGAGCG CATGTACCCG GAGGACGGCG CCCTGAAGGG
      841 CGAGATCAAG CAGCGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCCG AGGTCAAGAC
      901 CACCTACAAG GCCAAGAAGC CGGTCCAGCT GCCGGGCGCC TACAACGTGA ACATCAAGCT
      961 GGACATCACC AGCCACAACG AGGACTACAC CATCGTCGAG CAGTACGAGC GCGCCGAGGG
     1021 CCGCCACAGC ACCGGCGGCA TGGACGAGCT GTACAAGTGA AAGCTTATCG ATACCGTCGA
     1081 CCTCGAGGGG GGGCCCCCGG GGATCATCGA GCCGAGAACG TTATCGAAGT TGGTCATGTG
     1141 TAATCCCCTC GTTTGAACTT TGGATTAAGC GTAGATACAC CCTTGGACAA GCCAGTTGGA
     1201 TTCGGAGACA AGCAAATTCA GCCTTAAAAA GGGCGAGGCC CTGCGGTGGT GGAACACCGC
     1261 AGGGCCTCTA ACCGCTCGAC GCGCTGCACC AACCAGCCCG CGAACGGCTG GCAGCCAGCG
     1321 TAAGGCGCGG CTCATCGGGC GGCGTTCGCC ACGATGTCCT GCACTTCGAG CCAAGCCTCG
     1381 AACACCTGCT GGTGTGCACG ACTCACCCGG TTGTTGACAC CGCGCGCGGC CGTGCGGGCT
     1441 CGGTGGGGCG GCTCTGTCGC CCTTGCCAGC GTGAGTAGCG CGTACCTCAC CTCGCCCAAC
     1501 AGGTCGCACA CAGCCGATTC GTACGCCATA AAGCCAGGTG AGCCCACCAG CTCCGTAAGT
     1561 TCGGGCGCTG TGTGGCTCGT ACCCGCGCAT TCAGGCGGCA GGGGGTCTAA CGGGTCTAAG
     1621 GCGGCGTGTA CGGCCGCCAC AGCGGCTCTC AGCGGCCCGG AAACGTCCTC GAAACGACGC
     1681 ATGTGTTCCT CCTGGTTGGT ACAGGTGGTT GGGGGTGCTC GGCTGTCGCT GGTGTTCCAC
     1741 CACCAGGGCT CGACGGGAGA GCGGGGGAGT GTGCAGTTGT GGGGTGGCCC CTCAGCGAAA
     1801 TATCTGACTT GGAGCTCGTG TCGGACCATA CACCGGTGAT TAATCGTGGT CTACTACCAA
     1861 GCGTGAGCCA CGTCGCCGAC GAATTTGAGC AGCTCTGGCT GCCGTACTGG CCGCTGGCAA
     1921 GCGACGATCT GCTCGAGGGG ATCTACCGCC AAAGCCGCGC GTCGGCCCTA GGCCGCCGGT
     1981 ACATCGAGGC GAACCCAACA GCGCTGGCAA ACCTGCTGGT CGTGGACGTA GACCATCCAG
     2041 ACGCAGCGCT CCGAGCGCTC AGCGCCCGGG GGTCCCATCC GCTGCCCAAC GCGATCGTGG
     2101 GCAATCGCGC CAACGGCCAC GCACACGCAG TGTGGGCACT CAACGCCCCT GTTCCACGCA
     2161 CCGAATACGC GCGGCGTAAG CCGCTCGCAT ACATGGCGGC GTGCGCCGAA GGCCTTCGGC
     2221 GCGCCGTCGA CGGCGACCGC AGTTACTCAG GCCTCATGAC CAAAAACCCC GGCCACATCG
     2281 CCTGGGAAAC GGAATGGCTC CACTCAGATC TCTACACACT CAGCCACATC GAGGCCGAGC
     2341 TCGGCGCGAA CATGCCACCG CCGCGCTGGC GTCAGCAGAC CACGTACAAA GCGGCTCCGA
     2401 CGCCGCTAGG GCGGAATTGC GCACTGTTCG ATTCCGTCAG GTTGTGGGCC TATCGTCCCG
     2461 CCCTCATGCG GATCTACCTG CCGACCCGGA ACGTGGACGG ACTCGGCCGC GCGATCTATG
     2521 CCGAGTGCCA CGCGCGAAAC GCCGAATTCC CGTGCAACGA CGTGTGTCCC GGACCGCTAC
     2581 CGGACAGCGA GGTCCGCGCC ATCGCCAACA GCATTTGGCG TTGGATCACA ACCAAGTCGC
     2641 GCATTTGGGC GGACGGGATC GTGGTCTACG AGGCCACACT CAGTGCGCGC CAGTCGGCCA
     2701 TCTCGCGGAA GGGCGCAGCA GCGCGCACGG CGGCGAGCAC AGTTGCGCGG CGCGCAAAGT
     2761 CCGCGTCAGC CATGGAGGCA TTGCTATGAG CGACGGCTAC AGCGACGGCT ACAACCGGCA
     2821 GCCGACTGTC CGCAAAAAGC GGCGCGTGAC CGCCGCCGAA GGCGCTCGAA TCACCGGACT
     2881 ATCCGAACGC CACGTCGTCC GGCTCGTGGC GCAGGAACGC AGCGAGTGGC TCGCCGAGCA
     2941 GGCTGCACGC CGCGAACGCA TCCGCGCCTA TCACGACGAC GAGGGCCACT CTTGGCCGCA
     3001 AACGGCCAAA CATTTCGGGC TGCATCTGGA CACCGTTAAG CGACTCGGCT ATCGGGCGAG
     3061 GAAAGAGCGT GCGGCAGAAC AGGAAGCGGC TCAAAAGGCC CACAACGAAG CCGACAATCC
     3121 ACCGCTGTTC TAACGCAATT GGGGAGCGGG TGTCGCGGGG GTTCCGTGGG GGGTTCCGTT
     3181 GCAACGGGTC GGACAGGTAA AAGTCCTGGT AGACGCTAGT TTTCTGGTTT GGGCCATGCC
     3241 TGTCTCGTTG CGTGTTTCGT TGCGCCCGTT TTGAATACCA GCCAGACGTG GCTCCCTCAC
     3301 GATATCAATA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTATC TGTTGTTTGA
     3361 AAAAAAAAAG CGCCGCAACT GCGGCGCTTT TTTTTTTGAA TTCTCTGACC AGGGAAAATA
     3421 GCCCTCTGAC CTGGGGATTT GCGATCCCTA TCAGTGATAG ATATAATCTG GGAGGAGACG
     3481 ATTAATGCGT CTCGGTTTTT GTACTCGAAA GAAGCTACAA AGATAAGGCT TCATGCCGAA
     3541 ATCAACACCC TGTCATTTTA TGGCAGGGTG TTTTTTTTTT GTCGACTTGG GGACCCTAGA
     3601 GGTCCCCTTT TTTATTTTTT TGGATGAAGA GCATTATCAA TGGCTCTTCA CAGGAAGCTT
     3661 AAAAAAAAAA GCCCCGCGAT TGCGGGGCTT TTTTTTTTTA ATACGGTTAT CCACAGAATC
     3721 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
     3781 AAAGGCTGGC CACTCTCGTT GCACGGTTCG TTGTGCCGTT ACAGGCCCCG TTGACAGCTC
     3841 ACCGAACGTA GTTAAAACAT GCTGGTCAAA CTAGGTTTAC CAACGATACG AGTCAGCTCA
     3901 TCTAGGGCCA GTTCTAGGCG TTGTTCGTTG CGCGGTTCGT TGCGCATGTT TCGTGTGGTT
     3961 GCTAGATGGC TCCGCAACCA CACGCTTCGA GGTTGAGTGC TTCCAGCACG GGCGCGATCC
     4021 AGAAGAACTT CGTCGTGCGA CTGTCCTCGT TGATCCTTGC CGAGCTGGGA TGGAAGCCCG
     4081 GCCGACCCAC CCTGGAGGAG ATGATCGAGG ATGCCAGGGC CTTTCACGCC CGCCGCTGCT
     4141 GAGCGTCCGC CGCCGGGCCC GCACCGCCGT CGGCCGGCCC GCTCCGGGCT CGCAGCAGCG
     4201 GGCTTCGGCG CGGGCCCGGG GCTCCCGGGC CGCCGGGCGG GGCTCCGCCC GGCGGCCGCC
     4261 GGGGGCCGGG GGCGGCGCCG GGCGGCCCGG GGCGTCAGGC GCCGGGGGCG GTGTCCGGCG
     4321 GCCCCCAGAG GAACTGCGCC AGTTCCTCCG GATCGGTGAA GCCGGAGAGA TCCAGCGGGG
     4381 TCTCCTCGAA CACCTCGAAG TCGTGCAGGA AGGTGAAGGC GAGCAGTTCG CGGGCGAAGT
     4441 CCTCGGTCCG CTTCCACTGC GCCCCGTCGA GCAGCGCGGC CAGGATCTCG CGGTCGCCCC
     4501 GGAAGGCGTT GAGATGCAGT TGCACCAGGC TGTAGCGGGA GTCTCCCGCA TAGACGTCGG
     4561 TGAAGTCGAC GATCCCGGTG ACCTCGGTCG CGGCCAGGTC CACGAAGATG TTGGTCCCGT
     4621 GCAGGTCGCC GTGGACGAAC CGGGGTTCGC GGCCGGCCAG CAGCGTGTCC ACGTCCGGCA
     4681 GCCAGTCCTC CAGGCGGTCC AGCAGCCGGG GCGAGAGGTA GCCCCACCCG CGGTGGTCCT
     4741 CGACGGTCGC CGCGCGGCGT TCCCGCAGCA GTTCCGGGAA GACCTCGGAA TGGGGGGTGA
     4801 GCACGGTGTT CCCGGTCAGC GGCACCCTGT GCAGCCGGCC GAGCACCCGG CCGAGTTCGC
     4861 GGGCCAGGGC GAGCAGCGCG TTCCGGTCGG TCGTGCCGTC CATCGCGGAC CGCCAGGTGG
     4921 TGCCGGTCAT CCGGCTCATC ACCAGGTAGG GCCACGGCCA GGCTCCGGTG CCGGGCCGCA
     4981 GCTCGCCGCG GCCGAGGAGG CGGGGCACCG GCACCGGGGC GTCCGCCAGG ACCGCGTACG
     5041 CCTCCGACTC CGACGCGAGG CTCTCCGGAC CGCACCAGTG CTCGCCGAAC AGCTTGATCA
     5101 CCGGGCCGGG CTCGCCGACC AGTACGGGGT TGGTGCTCTC GCCGGGCACC CGCAGCACCG
     5161 GCGGCACCGG CAGCCCGAGC TCCTCCAGGG CTCGGCGGGC CAGCGGCTCC CAGAATTCCT
     5221 GGTCGTTCCG CAGGCTCGCG TAGGAATCAT CCGAATCAAT ACGGTCGAGA AGTAACAGGG
     5281 ATTCTTGTGT CACAGCGGAC CTCTATTCAC AGGGTACGGG CCGGCTTAAT TCCGCACGGC
     5341 CGGTCGCGAC ACGGCCTGTC CGCACCGCGG ATCAGGCGTT GACGATGACG GGCTGGTCGG
     5401 CCACGTCGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG
     5461 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG
     5521 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
     5581 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
     5641 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
     5701 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
     5761 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
     5821 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
     5881 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
     5941 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
     6001 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
     6061 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
     6121 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
     6181 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
     6241 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
     6301 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG
     6361 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG
     6421 AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT
     6481 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT
     6541 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG
     6601 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACATGATT ACCAGATCTG
     6661 GCTCGCACCG CGGTGGCGGC CGC
//
