LOCUS       pHIV-NAT-T2A-hCD       10427 bp DNA     circular SYN 22-MAY-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2024 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV Enhancer
                     /note="color: #c7b0e3"
     promoter        619..817
                     /label=CMV promoter
                     /note="color: #75c6a9"
     LTR             835..1015
                     /label=5%82%C4%F4 LTR truncated
                     /note="color: #84b0dc"
     misc_feature    1062..1187
                     /label=HIV
                     /note="color: #85dae9"
     misc_feature    1686..1919
                     /label=RRE
                     /note="color: #faac61"
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS (central polypurine tract/centraltermi
                     /label=cPPT/CTS (central polypurine 
                     tract/centraltermination sequence)
                     /note="color: #d6b295"
     promoter        2627..3805
                     /label=EF-1a promoter
                     /note="color: #b7e6d7"
     promoter        2858..3796
                     /label=EF-1a promoter intron A
                     /note="color: #9eafd2"
     intron          2858..3796
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     regulatory      3826..3835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    3826..3831
                     /label=Kozak sequence
                     /note="color: #85dae9"
     CDS             3832..6459
                     /codon_start=1
                     /label=eGFP-NFATC2IP-hGEM-GGGS-hSLBP ORF
                     /note="color: #c7b0e3"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKGAPAEPVGKRGRWSGGSGAGRGGRGGWGGRGRRPRAQRSPSR
                     GTLDVVSVDLVTDSDEEILEVATARGAADEVEVEPPEPPGPVASRDNSNSDSEGEDRRP
                     AGPPREPVRRRRRLVLDPGEAPLVPVYSGKVKSSLRLIPDDLSLLKLYPPGDEEEAELA
                     DSSGLYHEGSPSPGSPWKTKLRTKDKEEKKKTEFLDLDNSPLSPPSPRTKSRTHTRALK
                     KLSEVNKRLQDLRSCLSPKPPQGQEQQGQEDEVVLVEGPTLPETPRLFPLKIRCRADLV
                     RLPLRMSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKLGVADIIDCVVL
                     TSSPEATETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEAMGLSGRKLSFFFD
                     GTKLSGRELPADLGMESGDLIEVWGGYPSHWRPLEMNPSMKQKQEEIKENIKNSSVPRR
                     TLKMIQPSASGSLVGRENELSAGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQ
                     ESFDLMIKENPSSQYWKEVAEKRRKALGGGSASPPSPARWSLGRKRRADGRRWRPEDAE
                     EAEHRGAERRPESFTTPEGPKPRSRCSDWASAVEEDEMRTRVNKEMARYKRKLLINDFG
                     RERK"
     misc_feature    3832..4548
                     /label=eGFP
                     /note="color: #75c6a9"
     CDS             3832..4548
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3832 .. 3834 / #05fd14
                        2: 3835 .. 3837 / #05fd14 / 1a
                        3: 3838 .. 4548 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3832..4548
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="This feature has 3 segments:
                        1: 3832 .. 3834 / #05fc14
                        2: 3835 .. 3837 / #05fc14 / 1a
                        3: 3838 .. 4548 / #05fc14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4558..5811
                     /label=NFATC2IP ORF
                     /note="color: #ffef86"
     misc_feature    5812..5841
                     /label=Linker
                     /note="color: #85dae9"
     misc_feature    5842..6171
                     /label=hGEM(1-110)
                     /note="color: #faac61"
     CDS             5842..6171
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /note="color: #993366"
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    6172..6183
                     /label=Linker GGGS
                     /note="color: #ff9ccd"
     misc_feature    6184..6456
                     /label=SLBP(18-108)
                     /note="color: #c7b0e3"
     misc_feature    6196..6198
                     /label=S23
                     /note="note=""direct strand translation""translation=""S"""
                     /note="color: #d6b295"
     misc_feature    6214..6240
                     /label=NLS
                     /note="note=""direct strand 
                     translation""translation=""RKRR"""
                     /note="color: #b7e6d7"
     misc_feature    6283..6285
                     /label=G51
                     /note="note=""direct strand translation""translation=""G"""
                     /note="color: #b1ff67"
     misc_feature    6310..6315
                     /label=T60 T61
                     /note="note=""direct strand 
                     translation""translation=""TT"""
                     /note="color: #d6b295"
     misc_feature    6409..6435
                     /label=NLS
                     /note="note=""direct strand 
                     translation""translation=""KRKL"""
                     /note="color: #c6c9d1"
     misc_feature    6485..7071
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 6485 .. 7059 / #a6acb3
                        2: 7060 .. 7062 / #a6acb3 / ATG
                        3: 7063 .. 7071 / #a6acb3"
     misc_feature    6485..7059
                     /label=IRES2
                     /note="color: #ffef86"
     gene            7072..7650
                     /label=NatMX
                     /note="/translation=MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVT
                     ATGDGFTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVS
                     YSGWNRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAY
                     RRMGFTLCGLDTALYDGTASDGEQALYMSMPCPGSG"
                     /note="color: #b1ff67; direction: RIGHT"
     gene            7651..7704
                     /label=T2A
                     /note="/translation=EGRGSLLTCGDVEENPGP"
                     /note="color: #c6c9d1; direction: RIGHT"
     CDS             7651..7704
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 7701"
                     /translation="EGRGSLLTCGDVEENPGP"
     gene            7705..7890
                     /label=hCD52
                     /note="/translation=MKRFLFLLLTISLLVMVQIQTGLSGQNDTSQTSSPSASS
                     NISGGIFLFFVANAIIHLFCFS"
                     /note="color: #b1ff67; direction: RIGHT"
     primer_bind     complement(7907..7923)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             8433..8613
                     /label=5%82%C4%F4 LTR truncated
                     /note="color: #b7e6d7"
     rep_origin      8675..9263
                     /label=ori
                     /note="color: #b4abac"
     CDS             complement(9434..10294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9434 .. 10225 / #ccffcc
                        2: 10226 .. 10294 / #ccffcc / signal sequence
                     Cleavage site after base 10225"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            9434..10225
                     /label=AmpR
                     /note="color: #75c6a9; direction: RIGHT"
     promoter        10226..10399
                     /label=AmpR promoter
                     /note="color: #d59687"
     promoter        complement(10295..10399)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag ccgtgaggct ccggtgcccg
     2641 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2701 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2761 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2821 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2881 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2941 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3001 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3061 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3121 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3181 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3241 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3301 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3361 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3421 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3481 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3541 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3601 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3661 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3721 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3781 ttttcttcca tttcaggtgt cgtgagcggc cgctgaactg aattcgccac catggtgagc
     3841 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     3901 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     3961 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4021 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     4081 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4141 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4201 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4261 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     4321 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     4381 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     4441 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     4501 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cgcgccagcg
     4561 gagcctgtgg ggaagcgggg ccgctggtcc ggaggtagcg gtgccggccg agggggtcgg
     4621 ggcggctggg gcggtcgggg ccggcgtcct cgggcccagc ggtctccatc ccggggcacg
     4681 ctggacgtag tgtctgtgga cttggtcacc gacagcgatg aggaaattct ggaggtcgcc
     4741 accgctcgcg gtgccgcgga cgaggttgag gtggagcccc cggagccccc ggggccggtc
     4801 gcgtcccggg ataacagcaa cagtgacagc gaaggggagg acaggcggcc cgcaggaccc
     4861 ccgcgggagc cggtcaggcg gcggcggcgg ctggtgctgg atccggggga ggcgccgctg
     4921 gttccggtgt actcggggaa ggttaaaagc agccttcgcc ttatcccaga tgatctatcc
     4981 ctcctgaaac tctaccctcc aggggatgag gaagaggcag agctggcaga ttcgagtggt
     5041 ctctaccatg agggctcccc atcaccaggc tctccctgga agacaaagct gaggactaag
     5101 gataaagaag agaagaaaaa gacagagttt ctggatctgg acaactctcc tctgtcccca
     5161 ccttcaccaa ggaccaaaag cagaacgcat actcgggcac tcaagaagtt aagtgaggtg
     5221 aacaagcgcc tccaggatct ccgttcctgt ctgagcccca agccacctca gggtcaagag
     5281 caacagggcc aagaggatga agtggtcttg gtggaagggc ccaccctccc agagaccccc
     5341 cgactcttcc cactcaaaat ccgttgccgg gctgacctgg tcagattgcc cctcaggatg
     5401 tcggagcccc tgcagagtgt ggtggaccac atggccaccc accttggggt gtccccaagc
     5461 aggatccttt tgctttttgg agagacagag ctatcaccta ctgccactcc caggacccta
     5521 aagctcggag tggctgacat cattgactgt gtggtactaa caagttctcc agaggccaca
     5581 gagacgtccc aacagctcca gctccgggtg cagggaaagg agaaacacca gacactggaa
     5641 gtctcactgt ctcgagattc ccctctaaag accctcatgt cccactatga ggaggccatg
     5701 ggactgtcgg gacggaagct ctccttcttc tttgatggga caaagctttc aggcagggag
     5761 ctgccagctg acctgggcat ggaatctggg gacctcattg aggtctgggg cggatatcca
     5821 tcacactggc ggccgctcga gatgaatccc agtatgaagc agaaacaaga agaaatcaaa
     5881 gagaatataa agaatagttc tgtcccaaga agaactctga agatgattca gccttctgca
     5941 tctggatctc ttgttggaag agaaaatgag ctgtccgcag gcttgtccaa aaggaaacat
     6001 cggaatgacc acttaacatc tacaacttcc agccctgggg ttattgtccc agaatctagt
     6061 gaaaataaaa atcttggagg agtcacccag gagtcatttg atcttatgat taaagaaaat
     6121 ccatcctctc agtattggaa ggaagtggca gaaaaacgga gaaaggcgct gggcggggga
     6181 tccgccagcc cgccgtcccc cgcgcgatgg agcctgggac ggaagcgcag agccgacggc
     6241 aggcgctgga ggcccgaaga cgccgaggag gcagagcacc gcggcgccga gcgcagaccc
     6301 gagagcttta ccactcctga aggccctaaa ccccgttcca gatgctctga ctgggcaagt
     6361 gcagttgaag aagatgaaat gaggaccaga gttaacaaag aaatggcaag atataaaagg
     6421 aaactcctca tcaatgactt tggaagagag agaaaatgat ctagagtacc cgggctagga
     6481 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     6541 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     6601 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     6661 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     6721 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     6781 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     6841 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     6901 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     6961 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     7021 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catgggcacc
     7081 accctggacg acacggctta ccgataccgc accagtgtcc ctggggacgc cgaggccatc
     7141 gaggcactgg atgggtcctt caccaccgac accgtcttcc gcgtcaccgc caccggggac
     7201 ggcttcaccc tgcgggaggt gccggtggac ccgcccctga ccaaggtgtt ccccgacgac
     7261 gaatcggacg acgaatcgga cgacggggag gacggcgacc cggactcccg gacgttcgtc
     7321 gcgtacgggg acgacggcga cctggcgggc ttcgtggtcg tctcgtactc cggctggaac
     7381 cgccggctga ccgtcgagga cattgaggta gcacccgagc acaggggaca cggggtggga
     7441 cgggcattga tgggactggc taccgagttc gccagagaac ggggagctgg tcatctttgg
     7501 ctcgaagtca ccaacgtcaa cgcaccggcg atccacgctt accggcggat ggggttcacc
     7561 ctctgcggcc tggacaccgc cctgtacgac ggcaccgcct cggacggcga gcaggcgctc
     7621 tacatgtcca tgccctgccc cggcagtggc gagggcagag gaagtctgct aacatgcggt
     7681 gacgtcgagg agaatcctgg cccaatgaag cgcttcctct tcctcctact caccatcagc
     7741 ctcctggtta tggtacagat acaaactgga ctctcaggac aaaacgacac cagccaaacc
     7801 agcagcccct cagcatccag caacataagc ggaggcattt tccttttctt cgtggctaat
     7861 gccataatcc acctcttctg cttcagttga ggcgcgccaa atttatcgat accgtcgacc
     7921 tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     7981 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     8041 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8101 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     8161 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     8221 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     8281 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     8341 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     8401 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     8461 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8521 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8581 gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg
     8641 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8701 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8761 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8821 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8881 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8941 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9001 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9061 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9241 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9301 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9361 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9421 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9481 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9541 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9601 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9661 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9721 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9781 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9841 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9901 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9961 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10021 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10081 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10141 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10201 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10261 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
