LOCUS       pDONR223_NKX2-1_Short_V 3906 bp ds-DNA     circular     16-MAY-2024
DEFINITION  .
COMMENT     pDONR223
FEATURES             Location/Qualifiers
     misc_feature    1..100
                     /label="attL1 (fwd)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    95..102
                     /label="5' UTR"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     gene            103..1215
                     /label="NKX2-1 gene"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             103..1215
                     /label="Translation 103-1215"
                     /translation="MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPTAAMQQHAVGHHGAVTAAYHMTAAGVPQLSHSAVGGYCNGNLGNMSELPPYQDTMRNSASGPGWYGANPDPRFPAISRFMGPASGMNMSGMGGLGSLGDVSKNMAPLPSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQFKIWFQNHRYKMKRQAKDKAAQQQLQQDSGGGGGGGGTGCPQQQQAQQQSPRRVAVPVLVKDGKPCQAGAPAPGAASLQGHAQQQAQHQAQAAQAAAAAISVGSGGAGLGAHPGHQPGSAGQSPDLAHHAASPAALQGQVSSLSHLNSSGSDYGTMSCSTLLYGRTW"
     misc_feature    715..717
                     /label="V205F mutation"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1217..1316
                     /label="attL2 (rev)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1357..1373
                     /label="pDONR223 M13 Rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1804..2595
                     /label="SmR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3860..3882
                     /label="pDONR223 M13/pUC Fwd"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccATGTCGAT GAGTCCAAAG
      121 CAcacgactc cgttctcagt gtctgacatc ttgagtcccc tggaggaaag ctacaagaaa
      181 gtgggcatgg agggcggcgg cctcggggct ccgctggcgg cgtacaggca gggccaggcg
      241 gcaccgccaa cagcggccat gcagcagcac gccgtggggc accacggcgc cgtcaccgcc
      301 gcctaccaca tgacggcggc gggggtgccc cagctctcgc actccgccgt ggggggctac
      361 tgcaacggca acctgggcaa catgagcgag ctgccgccgt accaggacac catgaggaac
      421 agcgcctctg gccccggatg gtacggcgcc aacccagacc cgcgcttccc cgccatctcc
      481 cgcttcatgg gcccggcgag cggcatgaac atgagcggca tgggcggcct gggctcgctg
      541 ggggacgtga gcaagaacat ggccccgctg ccaagcgcgc cgcgcaggaa gcgccgggtg
      601 ctcttctcgc aggcgcaggt gtacgagctg gagcgacgct tcaagcaaca gaagtacctg
      661 tcggcgccgg agcgcgagca cctggccagc atgatccacc tgacgcccac gcagttcaag
      721 atctggttcc agaaccaccg ctacaaaatg aagcgccagg ccaaggacaa ggcggcgcag
      781 cagcaactgc agcaggacag cggcggcggc gggggcggcg ggggcaccgg gtgcccgcag
      841 cagcaacagg ctcagcagca gtcgccgcga cgcgtggcgg tgccggtcct ggtgaaagac
      901 ggcaaaccgt gccaggcggg tgcccccgcg ccgggcgccg ccagcctaca aggccacgcg
      961 cagcagcagg cgcagcacca ggcgcaggcc gcgcaggcgg cggcagcggc catctccgtg
     1021 ggcagcggtg gcgccggcct tggcgcacac ccgggccacc agccaggcag cgcaggccag
     1081 tctccggacc tggcgcacca cgccgccagc cccgcggcgc tgcagggcca ggtatccagc
     1141 ctgtcccacc tgaactcctc gggctcggac tacggcacca tgtcctgctc cacctTGCTA
     1201 TACGGTCGGA CCTGGttgcc aactttcttg tacaaagttg gcattataag aaagcattgc
     1261 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1321 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1381 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc
     1441 ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg
     1501 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
     1561 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     1621 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     1681 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     1741 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     1801 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1861 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1921 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1981 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     2041 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     2101 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     2161 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     2221 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     2281 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     2341 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     2401 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     2461 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     2521 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     2581 gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
     2641 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2701 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2761 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2821 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2881 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2941 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3001 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3061 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3121 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3181 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3241 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3301 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3361 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3421 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3481 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3541 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3601 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3661 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3721 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3781 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3841 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3901 gggccc
//