LOCUS       pDONR223_NKX2-1_HD(160- 2988 bp ds-DNA     circular     16-MAY-2024
DEFINITION  .
COMMENT     pDONR223
FEATURES             Location/Qualifiers
     misc_feature    1..100
                     /label="attL1 (fwd)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    95..102
                     /label="5' UTR"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     gene            103..297
                     /label="NKX2-1 gene"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             103..297
                     /label="Translation 103-297"
                     /translation="MPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDK"
     misc_feature    299..398
                     /label="attL2 (rev)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    439..455
                     /label="pDONR223 M13 Rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2942..2964
                     /label="pDONR223 M13/pUC Fwd"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccATGccgcg caggaagcgc
      121 cgggtgctct tctcgcaggc gcaggtgtac gagctggagc gacgcttcaa gcaacagaag
      181 tacctgtcgg cgccggagcg cgagcacctg gccagcatga tccacctgac gcccacgcag
      241 gtcaagatct ggttccagaa ccaccgctac aaaatgaagc gccaggccaa ggacaagttg
      301 ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
      361 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
      421 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
      481 ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag
      541 gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa
      601 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
      661 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      721 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
      781 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      841 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
      901 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
      961 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     1021 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     1081 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     1141 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     1201 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     1261 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     1321 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     1381 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     1441 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     1501 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     1561 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     1621 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc
     1681 tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     1741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     1921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2161 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2461 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2521 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2581 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     2641 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     2701 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     2761 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     2821 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     2881 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     2941 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//