LOCUS       pDONR223_NKX2-4         3855 bp ds-DNA     circular     16-MAY-2024
DEFINITION  .
COMMENT     pDONR223
FEATURES             Location/Qualifiers
     misc_feature    1..100
                     /label="attL1 (fwd)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    95..102
                     /label="5' UTR"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     gene            103..1164
                     /label="NKX2-4"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             103..1164
                     /label="Translation 103-1164"
                     /translation="MSLSPKHTTPFSVSDILSPIEETYKKFSGAMDGAPPGLGAPLGAAAAYRAPPPGPSSQAATVAGMQPSHAMAGHNAAAAAAAAAAAAAAAATYHMPPGVSQFPHGAMGSYCNGGLGNMGELPAYTDGMRGGAATGWYGANPDPRYSSISRFMGPSAGVNVAGMGSLTGIADAAKSLGPLHAAAAAAAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDKAAQQLQQEGGLGPPPPPPPSPRRVAVPVLVKDGKPCQNGASTPTPGQAGPQPPAPTPAPELEELSPSPPALHGPGGGLAALDAAAGEYSGGVLGANLLYGRTW"
     misc_feature    1166..1265
                     /label="attL2 (rev)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1306..1322
                     /label="pDONR223 M13 Rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1753..2544
                     /label="SmR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3809..3831
                     /label="pDONR223 M13/pUC Fwd"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccATGTCGTT GAGCCCAAAG
      121 CACACGACGC CCTTCTCCGT GTCCGACATC CTGAGCCCCA TCGAGGAGAC CTACAAGAAG
      181 TTCAGCGGCG CCATGGACGG CGCGCCACCC GGCCTGGGGG CGCCCCTGGG GGCCGCGGCC
      241 GCCTACCGCG CGCCGCCACC TGGGCCCTCC TCGCAGGCGG CGACCGTGGC GGGCATGCAG
      301 CCTTCTCACG CCATGGCGGG TCACAACgcg gcggccgcgg cggcggcggc agcagctgcg
      361 gcggcggcgg cCGCCACCTA CCACATGCCG CCCGGCGTCT CGCAGTTCCC GCACGGCGCC
      421 ATGGGCAGCT ACTGCAACGG CGGCCTGGGC AACATGGGCG AGCTGCCCGC CTACACGGAC
      481 GGCATGCGGG GCGGCGCGGC CACCGGCTGG TACGGCGCCA ACCCGGACCC ACGCTACTCG
      541 TCAATCTCCA GGTTCATGGG GCCGTCGGCG GGCGTGAATG TGGCCGGCAT GGGGTCGCTG
      601 ACCGGCATCG CGGACGCCGC CAAGTCGCTG GGCCCGCTGC ACGCGGCGGC GGCGGCAGCC
      661 GCTCCGCGAA GGAAGCGCCG CGTGCTCTTC TCGCAGGCGC AGGTCTACGA GCTGGAGCGG
      721 CGCTTCAAGC AGCAGAAGTA CCTGTCGGCG CCCGAGCGCG AGCACCTGGC CAGCATGATC
      781 CACCTGACGC CCACGCAGGT CAAGATCTGG TTCCAGAACC ACCGGTACAA GATGAAACGG
      841 CAGGCCAAGG ACAAGGCGGC GCAGCAGCTG CAGCAGGAGG GCGGCCTGGg cccgccgccg
      901 cctccgccgc cgtccccgcg ccgcgtGGCG GTGCCTGTGC TGGTCAAGGA CGGCAAGCCG
      961 TGCCAGAACG GCGCCAGCAC GCCCACCCCC GGCCAGGCCG GTCCGCAGCC GCCGGCCCCG
     1021 ACGCCAGCAC CTGAGCTGGA GGAGCTGTCG CCCAGCCCAC CCGCGCTGCA CGGCCCGGGG
     1081 GGCGGCCTGG CGGCCCTGGA CGCGGCCGCC GGGGAGTACA GCGGCGGCGT CCTGGGCGCC
     1141 AACCTGCTCT ATGGCAGGAC GTGGttgcca actttcttgt acaaagttgg cattataaga
     1201 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1261 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1321 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1381 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctgcccaag
     1441 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     1501 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     1561 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     1621 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1681 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1741 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1801 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1861 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     1921 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1981 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2041 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2101 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     2161 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2221 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     2281 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     2341 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     2401 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     2461 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     2521 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     2581 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2641 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2701 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2761 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     2821 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2881 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2941 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3001 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     3061 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3121 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3181 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3241 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3301 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3361 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3421 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3481 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     3541 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     3601 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     3661 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     3721 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     3781 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     3841 cttaagctcg ggccc
//