LOCUS       pDONR223_NKX2-5         3765 bp ds-DNA     circular     16-MAY-2024
DEFINITION  .
COMMENT     pDONR223
FEATURES             Location/Qualifiers
     misc_feature    1..100
                     /label="attL1 (fwd)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    95..102
                     /label="5' UTR"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     gene            103..1074
                     /label="NKX2-5"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             103..1074
                     /label="Translation 103-1074"
                     /translation="MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKELCALQKAVELEKTEADNAERPRARRRRKPRVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELVGLPPPPPPPARRIAVPVLVRDGKPCLGDSAPYAPAYGVGLNPYGYNAYPAYPGYGGAACSPGYSCTAAYPAGPSPAQPATAAANNNFVNFGVGDLNAVQSPGIPQSNSGVSTLHGIRAW"
     misc_feature    1076..1175
                     /label="attL2 (rev)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1216..1232
                     /label="pDONR223 M13 Rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1663..2454
                     /label="SmR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3719..3741
                     /label="pDONR223 M13/pUC Fwd"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccATGTTCCC CAGCCCTGCT
      121 CTCACGCCCA CGCCCTTCTC AGTCAAAGAC ATCCTAAACC TGGAACAGCA GCAGCGCAGC
      181 CTGGCTGCCG CCGGAGAGCT CTCTGCCCGC CTGGAGGCGA CCCTGGCGCC CTCCTCCTGC
      241 ATGCTGGCCG CCTTCAAGCC AGAGGCCTAC GCTGGGCCCG AGGCGGCTGC GCCGGGCCTC
      301 CCAGAGCTGC GCGCAGAGCT GGGCCGCGCG CCTTCACCGG CCAAGTGTGC GTCTGCCTTT
      361 CCCGCCGCCC CCGCCTTCTA TCCACGTGCC TACAGCGACC CCGACCCAGC CAAGGACCCT
      421 AGAGCCGAAA AGAAAGAGCT GTGCGCGCTG CAGAAGGCGG TGGAGCTGGA GAAGACAGAG
      481 GCGGACAACG CGGAGCGGCC CCGGGCGCGA CGGCGGAGGA AGCCGCGCGT GCTCTTCTCG
      541 CAGGCGCAGG TCTATGAGCT GGAGCGGCGC TTCAAGCAGC AGCGGTACCT GTCGGCCCCC
      601 GAACGCGACC AGCTGGCCAG CGTGCTGAAA CTCACGTCCA CGCAGGTCAA GATCTGGTTC
      661 CAGAACCGGC GCTACAAGTG CAAGCGGCAG CGGCAGGACC AGACTCTGGA GCTGGTGGGG
      721 CTgcccccgc cgccgccgcc gcctgcccgc AGGATCGCGG TGCCAGTGCT GGTGCGCGAT
      781 GGCAAGCCAT GCCTAGGGGA CTCGGCGCCC TACGCGCCTG CCTACGGCGT GGGCCTCAAT
      841 CCCTACGGTT ATAACGCCTA CCCCGCCTAT CCGGGTTACG GCGGCGCGGC CTGCAGCCCT
      901 GGCTACAGCT GCACTGCCGC TTACCCCGCC GGGCCTTCCC CAGCGCAGCC GGCCACTGCC
      961 GCCGCCAACA ACAACTTCGT GAACTTCGGC GTCGGGGACT TGAATGCGGT TCAGAGCCCC
     1021 GGGATTCCGC AGAGCAACTC GGGAGTGTCC ACGCTGCATG GTATCCGAGC CTGGttgcca
     1081 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1141 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1201 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1261 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     1321 agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg
     1381 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     1441 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1501 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     1561 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1621 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     1681 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1741 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     1801 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     1861 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     1921 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     1981 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2041 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2101 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2161 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2221 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2281 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2341 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2401 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     2461 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     2941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3241 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3301 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3361 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3421 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3481 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3541 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3601 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3661 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3721 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//