LOCUS       pLX309_NKX2-1(S)_V5-GFP 9442 bp ds-DNA     circular     16-MAY-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1..227
                     /label="RSV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             228..408
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    455..580
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1073..1306
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        1766..1976
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1788..1984
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      1835..1970
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2016..2618
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2016..2618
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS*"
     misc_feature    2661..2778
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2836..3236
                     /label="SFFV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: SFFV"
     misc_feature    3286..3306
                     /label="Gateway recomb1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     3286..3306
                     /label="attB1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3286..3311
                     /label="5' UTR"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     gene            3312..4424
                     /label="NKX2-1 gene"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3312..5279
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPTAAMQQHAVGHHGAVTAAYHMTAAGVPQLSHSAVGGYCNGNLGNMSELPPYQDTMRNSASGPGWYGANPDPRFPAISRFMGPASGMNMSGMGGLGSLGDVSKNMAPLPSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDKAAQQQLQQDSGGGGGGGGTGCPQQQQAQQQSPRRVAVPVLVKDGKPCQAGAPAPGAASLQGHAQQQAQHQAQAAQAAAAAISVGSGGAGLGAHPGHQPGSAGQSPDLAHHAASPAALQGQVSSLSHLNSSGSDYGTMSCSTLLYGRTWLPTFLYKVVTGGKPIPNPLLGLDSTTRAEAAAKEAAAKEAAAKAGSVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITMGMDELYK*"
     misc_feature    4429..4449
                     /label="attB2 (rev)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4429..4449)
                     /label="Gateway Recomb2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_recomb     complement(4429..4449)
                     /label="attB2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4458..4499
                     /label="V5 tag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 product: epitope tag from simian virus 5 transl_table: 1 translation: GKPIPNPLLGLDST"
     CDS             4506..4556
                     /label="Rigid Linker"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 label: Rigid Linker transl_table: 1 translation: AEAAAKEAAAKEAAAKA"
     CDS             4563..5276
                     /label="GFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 transl_table: 1 translation: VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    5295..5541
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5542..5883
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5766..5777)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     LTR             5955..6188
                     /label="3' LTR (ΔU3)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    6266..6387
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      6427..6562
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(6583..6601)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(6611..6627)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     rep_origin      6769..7224
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        7250..7354
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             7355..7423
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             7424..8215
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      8386..8974
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    9262..9283
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        9298..9303
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        9304..9321
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        9322..9328
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    9336..9352
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     primer_bind     9360..9376
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     promoter        9397..9415
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 cTCGAGGCCT GCAGGAGATA GCTGCAGTAA CGCCATTTTG CAAGGCATGG AAAAATACCA
     2881 AACCAAGAAT AGAGAAGTTC AGATCAAGGG CGGGTACATG AAAATAGCTA ACGTTGGGCC
     2941 AAACAGGATA TCTGCGGTGA GCAGTTTCGG CCCCGGCCCG GGGCCAAGAA CAGATGGTCA
     3001 CCGCAGTTTC GGCCCCGGCC CGAGGCCAAG AACAGATGGT CCCCAGATAT GGCCCAACCC
     3061 TCAGCAGTTT CTTAAGACCC ATCAGATGTT TCCAGGCTCC CCCAAGGACC TGAAATGACC
     3121 CTGCGCCTTA TTTGAATTAA CCAATCAGCC TGCTTCTCGC TTCTGTTCGC GCGCTTCTGC
     3181 TTCCCGAGCT CTATAAAAGA GCTCACAACC CCTCACTCGG CGCGCCAGTC CTCCGACAGA
     3241 CTGAGTCGGC CGGTCGAATC AAGCTTGcta gcatcgattg atcaacaagt ttgtacaaaa
     3301 aagttggcac catgtcgatg agtccaaagc acacgactcc gttctcagtg tctgacatct
     3361 tgagtcccct ggaggaaagc tacaagaaag tgggcatgga gggcggcggc ctcggggctc
     3421 cgctggcggc gtacaggcag ggccaggcgg caccgccaac agcggccatg cagcagcacg
     3481 ccgtggggca ccacggcgcc gtcaccgccg cctaccacat gacggcggcg ggggtgcccc
     3541 agctctcgca ctccgccgtg gggggctact gcaacggcaa cctgggcaac atgagcgagc
     3601 tgccgccgta ccaggacacc atgaggaaca gcgcctctgg ccccggatgg tacggcgcca
     3661 acccagaccc gcgcttcccc gccatctccc gcttcatggg cccggcgagc ggcatgaaca
     3721 tgagcggcat gggcggcctg ggctcgctgg gggacgtgag caagaacatg gccccgctgc
     3781 caagcgcgcc gcgcaggaag cgccgggtgc tcttctcgca ggcgcaggtg tacgagctgg
     3841 agcgacgctt caagcaacag aagtacctgt cggcgccgga gcgcgagcac ctggccagca
     3901 tgatccacct gacgcccacg caggtcaaga tctggttcca gaaccaccgc tacaaaatga
     3961 agcgccaggc caaggacaag gcggcgcagc agcaactgca gcaggacagc ggcggcggcg
     4021 ggggcggcgg gggcaccggg tgcccgcagc agcaacaggc tcagcagcag tcgccgcgac
     4081 gcgtggcggt gccggtcctg gtgaaagacg gcaaaccgtg ccaggcgggt gcccccgcgc
     4141 cgggcgccgc cagcctacaa ggccacgcgc agcagcaggc gcagcaccag gcgcaggccg
     4201 cgcaggcggc ggcagcggcc atctccgtgg gcagcggtgg cgccggcctt ggcgcacacc
     4261 cgggccacca gccaggcagc gcaggccagt ctccggacct ggcgcaccac gccgccagcc
     4321 ccgcggcgct gcagggccag gtatccagcc tgtcccacct gaactcctcg ggctcggact
     4381 acggcaccat gtcctgctcc acctTGCTAT ACGGTCGGAC CTGGttgcca actttcttgt
     4441 acaaagtggT taccggtGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGA
     4501 CGCGTgctga agctgctgca AAGgaagctg cagctAAGga ggctgcagct AAGgctGGAT
     4561 CCGTCAGTAA AGGGGAAGAA TTGTTTACGG GTGTTGTTCC GATACTTGTT GAATTGGATG
     4621 GGGATGTGAA TGGTCATAAA TTTTCCGTTA GTGGTGAAGG GGAAGGTGAC GCTACTTATG
     4681 GTAAATTGAC ATTGAAATTT ATTTGTACTA CAGGAAAACT TCCAGTCCCA TGGCCGACTT
     4741 TGGTAACGAC TCTTACATAT GGAGTCCAAT GTTTTAGTAG GTATCCAGAT CACATGAAAC
     4801 AACATGATTT CTTTAAATCA GCAATGCCTG AGGGATATGT TCAAGAACGA ACAATATTCT
     4861 TTAAAGATGA TGGGAACTAT AAGACAAGGG CAGAAGTCAA ATTTGAAGGT GATACACTCG
     4921 TTAATCGGAT TGAACTGAAA GGGATAGATT TTAAGGAAGA TGGGAATATT CTTGGGCACA
     4981 AACTTGAATA TAATTATAAT TCCCATAATG TATACATAAT GGCTGACAAG CAGAAGAATG
     5041 GAATTAAAGT CAATTTTAAA ATAAGGCATA ATATAGAAGA TGGGAGTGTC CAATTGGCTG
     5101 ATCATTACCA ACAAAATACA CCTATTGGGG ATGGTCCTGT CCTCTTGCCT GATAATCATT
     5161 ATCTTTCTAC ACAATCTGCT CTCTCAAAGG ACCCTAATGA AAAGCGCGAC CATATGGTGT
     5221 TGTTGGAATT TGTCACTGCT GCAGGCATTA CCATGGGAAT GGATGAACTC TATAAATGAa
     5281 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5341 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5401 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5461 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5521 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5581 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5641 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5701 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5761 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5821 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5881 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5941 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6001 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6061 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6121 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6181 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6241 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6301 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6361 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6421 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6481 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6541 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     6601 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6661 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6721 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     6781 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6841 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6901 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6961 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7021 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7081 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7141 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7201 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7261 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7321 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7381 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7441 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7501 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7561 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7621 cggtcgccgc atactctatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7681 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7741 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7801 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7861 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7921 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7981 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8041 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8101 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8161 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8221 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8281 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8521 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9061 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9121 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9181 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9241 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9301 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9361 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9421 aaagctggag ctgcaagctt aa
//