LOCUS       sequence-477226-        7594 bp    DNA     circular SYN 26-JUN-2025
DEFINITION  A recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR,
            CIBN and mCherry as expression marker. hSynapsin promoter for 
            panneuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7594)
  TITLE     PiGM-Iq system plasmids
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-hSyn-PiGM-Iq(mCherry)
FEATURES             Location/Qualifiers
     source          1..7594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   381..510
                     /label=AAV2 ITR
     promoter        592..1039
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3119..3172
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3734..4441
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3879..3897)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4147..4166
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4572..4592)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   5019..5148
                     /label=AAV2 ITR
     rep_origin      5329..5784
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5416..5435)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5626..5647
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5810..5914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5915..5983,5984..6775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6133..6152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6946..7534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7435..7454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgc
      181 gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg acctttggtc
      241 gcccgctcag ygggcacgck rggckwamca ccacacccgc cgcgcttaat gcgccgctac
      301 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      361 cctcttcgct attacgccag ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc
      421 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      481 gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact
      541 tatctacgta gccatgctct aggaagatct ctgcagaggg ccctgcgtat gagtgcaagt
      601 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
      661 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
      721 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
      781 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
      841 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
      901 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
      961 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     1021 gtcgtgcctg agagcgcagt cgaattcaag cttgggatcc atgactcctg ggaagcccaa
     1081 aactggcaag aaaagcaaac agcaaacttt tatcaagcct tctcctgagg aagcgctgct
     1141 ctgggcagaa gcatttgatg aactgctggc cagcaaatat gggcttgctg cattcagagc
     1201 gtttttaaag tccgagttct gtgaagaaaa tattgaattc tggttggctt gcgaagactt
     1261 caagaaaacc aaatcaccac agaaactgtc ctcaaaagca aggaaaatat acaccgactt
     1321 catcgagaag gaagctccca aagagataaa catagacttt caaacaaaaa ctctgattgc
     1381 ccaaaatatc caagaggcta caagtggctg cttcaccaca gcgcagaaga gggtgtacag
     1441 cctgatggag aacaactctt atcctcgttt cttggaatca gaattttatc aggacttgtg
     1501 taaaaagcca cagatcacca cggagcccca tgctacaacc ggtggcggca gtggtggcgg
     1561 cagcatgaag atggacaaga agacaattgt ctggtttcgc agggacctga gaatcgaaga
     1621 taaccccgca ttggcagcag cagcccatga gggttctgtg ttccctgtct tcatttggtg
     1681 tccagaggag gagggacagt tctaccctgg ccgggccagc agatggtgga tgaagcagtc
     1741 cttggctcac ctgtcacaga gtctcaaggc actgggctcc gatctgacgt tgattaagac
     1801 ccacaacacg attagcgcaa ttctggactg catccgggtg accggcgcaa caaaagtggt
     1861 tttcaaccac ctctacgacc cagttagcct tgtgcgggac cataccgtca aggagaaatt
     1921 ggtggagcgg ggcatcagtg tgcagtctta caatggcgac ctgttgtatg aaccatggga
     1981 aatttattgt gagaaaggca agcctttcac tagcttcaat tcttactgga aaaaatgtct
     2041 ggacatgagt atcgaaagcg tgatgcttcc ccccccctgg cggctgatgc caattactgc
     2101 cgccgctgag gcaatctggg catgtagcat cgaagagttg ggtctggaga atgaggctga
     2161 gaagccaagt aacgccctgc tgactcgcgc atggtcccca ggatggtcaa acgcagacaa
     2221 acttctgaac gagttcattg aaaagcagct gattgactac gctaagaact ccaaaaaagt
     2281 cgttggaaac tcaacaagcc tcttgagccc ttacctgcac tttggcgaga tctctgttcg
     2341 gcacgtgttc cagtgcgccc ggatgaagca gataatctgg gcaagagaca aaaacagtga
     2401 gggagaggag tctgccgatt tgttcctgag aggcatcgga ctccgggagt acagccgcta
     2461 catttgtttt aacttccctt tcacccacga acagagcctc ttgtctcacc tgcgcttctt
     2521 cccatgggac gcagatgttg ataaattcaa ggcttggcga caggggcgca ccgggtatcc
     2581 acttgtcgac gctggcatga gggagctgtg ggccacgggg tggatgcata ataggatcag
     2641 agtgattgtg tcttctttcg ccgtcaagtt cttgttgctc ccatggaaat ggggcatgaa
     2701 gtacttctgg gacacgctgc tggacgctga ccttgagtgc gacattcttg ggtggcagta
     2761 catttccgga tctattcctg atgggcacga actcgatagg ctcgacaacc ccgctttgca
     2821 gggggctaaa tacgacccag aaggggaata catccgccag tggctccccg agctggcaag
     2881 gctgcctacc gagtggattc atcatccctg ggacgcaccg ttgactgttt tgaaggcaag
     2941 cggcgtagag ttgggtacca attacgcaaa gcctatcgtc gatattgaca ccgcaaggga
     3001 gctgctggca aaggctatct ctaggacacg ggaggcccaa attatgatcg gcgccgcctc
     3061 aggaggaggc tcaggaggag gaggcgcggg agctagcgga ggtggatctg gtggcggtga
     3121 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctcgtacggg
     3181 cggcagtggt ggcggcagca tgaatggcgc catcggtggc gaccttctcc tgaactttcc
     3241 agacatgagc gttcttgagc gacagagggc tcatctcaaa tacctgaacc cgacgttcga
     3301 tagtcccttg gctggattct ttgctgattc ttctatgatt accggcggcg aaatggattc
     3361 ttatctgagt actgctggtt tgaacctgcc tatgatgtac ggagagacca cagtggaggg
     3421 agatagcaga ctgtccatct ccccagagac cacgctgggc acaggaaact tcaaagcagc
     3481 caagtttgat acagagacca aagactgtaa tgaagccgct aaaaagatga ccatgaaccg
     3541 ggacgatctt gtggaagagg gcgaggaaga gaagtcaaag attacagaac agaacaatgg
     3601 ttccaccaag tcaatcaaaa aaatgaagca caaggccaaa aaagaggaaa ataatttttc
     3661 caacgattcc agcaaagtga caaaggaact ggagaagacc gactacattg gaggaggagg
     3721 cgcgggaatc gatatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     3781 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3841 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3901 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     3961 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     4021 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     4081 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     4141 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     4201 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     4261 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     4321 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     4381 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     4441 ggatagtgct ggtagtgaat tcggtaaaaa gaagaaaaag aagtcaaaga caaagtgtgt
     4501 aattatgtaa tctagaataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4561 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4621 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt
     4681 cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4741 ttgggcactg acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt
     4801 gtaaccatca ctagttaggc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4861 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     4921 ttcaggggga gatgtgggag gttttttaaa atcgataagg atcttcctag agcatggcta
     4981 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt
     5041 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     5101 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcattaatga
     5161 atcggccaac gcgcggggag aggcggkttg cgtattgggm gctcttccgc ctcccggkcw
     5221 ytgcccgggc ggcctcagtg agcgagcgag cgcgcagctg gcgtaatagc gaagaggccc
     5281 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     5341 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5401 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5461 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5521 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5581 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5641 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5701 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5761 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5881 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5941 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6001 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6061 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6121 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6181 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6241 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6301 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6361 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6421 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6481 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6541 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6601 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6661 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6721 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6781 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6841 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7081 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7321 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7561 gttcctggcc ttttgctggc cttttgctca catg
//
