LOCUS       11Feb2025_AAV_hS        7079 bp ds-DNA     linear       12-FEB-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    5073..5126
                     /vntifkey="21"
                     /locus_tag="T2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
                     /locus_tag="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5052..5072
                     /vntifkey="21"
                     /locus_tag="GSG\linker"
                     /ApEinfo_fwdcolor="#ffbe7d"
                     /ApEinfo_revcolor="#ffbe7d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2946..2951
                     /locus_tag="HindIII"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2..590)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2952..3398
                     /locus_tag="RGS2(149) (delta 1-63)"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#0080c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5127..5132
                     /locus_tag="BsiWI"
                     /ApEinfo_fwdcolor="#ff0514"
                     /ApEinfo_revcolor="#ff0514"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(82..101)
                     /note="pBR322 origin, forward primer"
                     /locus_tag="pBR322ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6289..6309)
                     /note="WPRE, reverse primer"
                     /locus_tag="WPRE-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3405..3413
                     /locus_tag="N-terminal linker"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(761..1621)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     I
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     S
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     W
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     A
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     S LIKHW"
                     /locus_tag="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_region   6736..6865
                     /locus_tag="AAV2 ITR"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5418..6134
                     /locus_tag="eGFP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1384..1403
                     /note="Ampicillin resistance gene, reverse primer"
                     /locus_tag="Amp-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          6180..7079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
                     /locus_tag="source:synthetic DNA construct(1)"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5397..5411
                     /locus_tag="C-terminal linker"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6135..6179
                     /locus_tag="polyK-CaaX-membrane anchor"
                     /ApEinfo_fwdcolor="#4444ff"
                     /ApEinfo_revcolor="#4444ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(1622..1726)
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5133..5153
                     /locus_tag="N-terminal linker(1)"
                     /ApEinfo_label="N-terminal linker"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1752..2207)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /locus_tag="f1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5154..5396
                     /locus_tag="oCIBN"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1889..1910)
                     /note="F1 origin, forward primer"
                     /locus_tag="F1ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3399..3404
                     /locus_tag="Age1"
                     /ApEinfo_fwdcolor="#a8ffa8"
                     /ApEinfo_revcolor="#a8ffa8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2101..2120
                     /note="F1 origin, reverse primer"
                     /locus_tag="F1ori-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5046..5051
                     /locus_tag="Nhe1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_region   2279..2408
                     /locus_tag="AAV2 ITR(1)"
                     /ApEinfo_label="AAV2 ITR"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5412..5417
                     /locus_tag="Cla1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3414..5018
                     /locus_tag="CRY2(535)"
                     /ApEinfo_fwdcolor="#e8e800"
                     /ApEinfo_revcolor="#e8e800"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2469..2941
                     /locus_tag="hSyn"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="#80ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2940..2945
                     /locus_tag="EcoR1"
                     /ApEinfo_fwdcolor="#ffb5b5"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5019..5045
                     /locus_tag="NES (nuclear export signal)"
                     /ApEinfo_fwdcolor="#ccffcc"
                     /ApEinfo_revcolor="#ccffcc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6688..6688
                     /locus_tag="SNP; no effect"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 atttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
       61 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      121 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      181 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      241 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      301 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      361 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      421 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      481 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      541 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      601 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      661 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      721 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      781 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      841 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      901 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
      961 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1021 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1081 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1141 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1201 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1261 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1321 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1381 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1441 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1501 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1561 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1621 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1681 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1741 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1801 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     1861 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     1921 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     1981 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2041 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2101 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2161 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2221 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2281 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2341 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2401 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2461 gaagatctct gcagagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg
     2521 cggggtgggg gtgcctacct gacgaccgac cccGacccac tggacaagca cccaaccccc
     2581 attccccaaa ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc
     2641 gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg
     2701 cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact
     2761 ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac
     2821 gcgaggcgcg agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca
     2881 gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg
     2941 aATTCAAGCT Tatgttcacc cgcgccgtga gccgactgag caggaagcgg ccgccgtctg
     3001 atatccacga cggcgatggg agctcaagca gcGgccacca gagcctcaag agcacagcca
     3061 agtgggcgtc ctccctggag aatcttctgg aagatccaga gggcgtgaag agattcaggg
     3121 aatttctgaa aaaggaattc agtgaggaaa acgtcttgtt ttggctagcg tgtgaagatt
     3181 tcaagaaaac ggaggacaag aagcagatgc aggaaaaggc caagaagatc tacatgacct
     3241 tcctgtccaa taaggcctct tcacaagtca atgtggaggg gcagtctcgg ctcactgaaa
     3301 agattctgga agaaccacac cctctgatgt tccaaaagct ccaggaccag atcttcaatc
     3361 tcatgaagta tgacagctac agccgcttct tgaagtctac cggtGGCGGC AGCATGAAGA
     3421 TGGACAAGAA GACAATTGTC TGGTTTCGCA GGGACCTGAG AATCGAAGAT AACCCCGCAT
     3481 TGGCAGCAGC AGCCCATGAG GGTTCTGTGT TCCCTGTCTT CATTTGGTGT CCAGAGGAGG
     3541 AGGGACAGTT CTACCCTGGC CGGGCCAGCA GATGGTGGAT GAAGCAGTCC TTGGCTCACC
     3601 TGTCACAGAG TCTCAAGGCA CTGGGCTCCG ATCTGACGTT GATTAAGACC CACAACACGA
     3661 TTAGCGCAAT TCTGGACTGC ATCCGGGTGA CCGGcGCAAC AAAAGTGGTT TTCAACCACC
     3721 TCTACGACCC AGTTAGCCTT GTGCGGGACC ATACCGTCAA GGAGAAATTG GTGGAGCGGG
     3781 GCATCAGTGT GCAGTCTTAC AATGGCGACC TGTTGTATGA ACCATGGGAA ATTTATTGTG
     3841 AGAAAGGCAA GCCTTTCACT AGCTTCAATT CTTACTGGAA AAAATGTCTG GACATGAGTA
     3901 TCGAAAGCGT GATGCTTCCC CCCCCCTGGC GGCTGATGCC AATTACTGCC GCCGCTGAGG
     3961 CAATCTGGGC ATGTAGCATC GAAGAGTTGG GTCTGGAGAA TGAGGCTGAG AAGCCAAGTA
     4021 ACGCCCTGCT GACTCGCGCA TGGTCCCCAG GATGGTCAAA CGCAGACAAA CTTCTGAACG
     4081 AGTTCATTGA AAAGCAGCTG ATTGACTACG CTAAGAACTC CAAAAAAGTC GTTGGAAACT
     4141 CAACAAGCCT CTTGAGCCCT TACCTGCACT TTGGCGAGAT CTCTGTTCGG CACGTGTTCC
     4201 AGTGCGCCCG GATGAAGCAG ATAATCTGGG CAAGAGACAA AAACAGTGAG GGAGAGGAGT
     4261 CTGCCGATTT GTTCCTGAGA GGCATCGGAC TCCGGGAGTA CAGCCGCTAC ATTTGTTTTA
     4321 ACTTCCCTTT CACCCACGAA CAGAGCCTCT TGTCTCACCT GCGCTTCTTC CCATGGGACG
     4381 CAGATGTTGA TAAATTCAAG GCTTGGCGAC AGGGGCGCAC CGGGTATCCA CTTGTCGACG
     4441 CTGGCATGAG GGAGCTGTGG GCCACGGGGT GGATGCATAA TAGGATCAGA GTGATTGTGT
     4501 CTTCTTTCGC CGTCAAGTTC TTGTTGCTCC CATGGAAATG GGGCATGAAG TACTTCTGGG
     4561 ACACGCTGCT GGACGCTGAC CTTGAGTGCG ACATTCTTGG GTGGCAGTAC ATTTCCGGAT
     4621 CTATTCCTGA TGGGCACGAA CTCGATAGGC TCGACAACCC CGCTTTGCAG GGGGCTAAAT
     4681 ACGACCCAGA AGGGGAATAC ATCCGCCAGT GGCTCCCCGA GCTGGCAAGG CTGCCTACCG
     4741 AGTGGATTCA TCATCCCTGG GACGCACCGT TGACTGTTTT GAAGGCaAGC GGCGTAGAGT
     4801 TGGGTACCAA TTACGCAAAG CCTATCGTCG ATATTGACAC CGCAAGGGAG CTGCTGGCAA
     4861 AGGCTATCTC TAGGACACGG GAGGCCCAAA TTATGATCGG CGCCGCCcct gatgagattg
     4921 tagcagatag cttcgaggcc ttaggggcta ataccattaa agaacctggt ctttgcccat
     4981 ctgtgtcttc taatgaccaa caagtacctt cggctgtcgA TctTAAGTTG GCTGGACTTG
     5041 ATATTGCTAG Cggaggtgga tctggtggcg gtgagggcag aggaagtctt ctaacatgcg
     5101 gtgacgtgga ggagaatccc ggccctCGTA CGGGCGGCAG TGGTGGCGGC AGCATGAATG
     5161 GCGCCATCGG TGGCGACCTT CTCCTGAACT TTCCAGACAT GAGCGTTCTT GAGCGACAGA
     5221 GGGCTCATCT CAAATACCTG AACCCGACGT TCGATAGTCC CTTGGCTGGA TTCTTTGCTG
     5281 ATTCTTCTAT GATTACCGGC GGCGAAATGG ATTCTTATCT GAGTACTGCT GGTTTGAACC
     5341 TGCCTATGAT GTACGGAGAG ACCACAGTGG AGGGAGATAG CAGACTGTCC ATCTCCGGAG
     5401 GAggCGCGgg AATCGATATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
     5461 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
     5521 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
     5581 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT
     5641 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
     5701 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
     5761 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
     5821 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG
     5881 CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
     5941 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
     6001 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA
     6061 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
     6121 ACGAGctgta taagggtaaa aagaagaaaa agaagtcaaa gacaaagtgt gtaattatgt
     6181 aagaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agaataatca
     6241 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6301 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6361 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
     6421 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6481 gtttatttgt gaaatttgtg atgctattgc tttatttgta accatcacta gttaggcttt
     6541 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     6601 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     6661 ttttaaaatc gataaggatc ttcctagggc atggctacgt agataagtag catggcgggt
     6721 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     6781 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     6841 cctcagtgag cgagcgagcg cgcagctgca ttaatgaatc ggccaacgcg cggggagagg
     6901 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6961 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7021 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgta
//
