LOCUS       AAV2_hSyn_IvfChr        6025 bp    DNA        circular     08-OCT-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    570..1041
                     /locus_tag="hSyn"
                     /label="hSyn"
                     /ApEinfo_label="hSyn"
                     /ApEinfo_fwdcolor="#a3ff98"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1839..1859
                     /locus_tag="Chrimson"
                     /label="Chrimson"
                     /ApEinfo_label="Chrimson"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1046..1051
                     /locus_tag="HindIII"
                     /label="HindIII"
                     /ApEinfo_label="HindIII"
                     /ApEinfo_fwdcolor="#fffba2"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(1302..1583,1587..1838)
                     /locus_tag="Chrimson(1)"
                     /label="Chrimson(1)"
                     /ApEinfo_label="Chrimson"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4347..5206
                     /locus_tag="Amp"
                     /label="Amp"
                     /ApEinfo_label="Amp"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2115..2174
                     /locus_tag="TS"
                     /label="TS"
                     /ApEinfo_label="TS"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    378..509
                     /locus_tag="5'ITR"
                     /label="5'ITR"
                     /ApEinfo_label="5'ITR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1839..1841
                     /locus_tag="Y260F"
                     /label="Y260F"
                     /ApEinfo_label="Y260F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3454..3584
                     /locus_tag="3' ITR"
                     /label="3' ITR"
                     /ApEinfo_label="3' ITR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2175..2891
                     /locus_tag="Citrine"
                     /label="Citrine"
                     /ApEinfo_label="Citrine"
                     /ApEinfo_fwdcolor="#efff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3766..4083
                     /locus_tag="f1 origin"
                     /label="f1 origin"
                     /ApEinfo_label="f1 origin"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1857..1859
                     /locus_tag="S266M"
                     /label="S266M"
                     /ApEinfo_label="S266M"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2901..2927
                     /locus_tag="WPRE-start"
                     /label="WPRE-start"
                     /ApEinfo_label="WPRE-start"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1059..1067
                     /locus_tag="Kozak seq"
                     /label="Kozak seq"
                     /ApEinfo_label="Kozak seq"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1659..1661
                     /locus_tag="M199C"
                     /label="M199C"
                     /ApEinfo_label="M199C"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2901..3193
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1062..1298
                     /locus_tag="ChIEF"
                     /label="ChIEF"
                     /ApEinfo_label="ChIEF"
                     /ApEinfo_fwdcolor="#9dff6c"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1052..1057
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#7cff9f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1566..1568
                     /locus_tag="C170"
                     /label="C170"
                     /ApEinfo_label="C170"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2895..2900
                     /locus_tag="XbaI"
                     /label="XbaI"
                     /ApEinfo_label="XbaI"
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1860..2114
                     /locus_tag="Chrimson(2)"
                     /label="Chrimson(2)"
                     /ApEinfo_label="Chrimson"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3200..3385
                     /locus_tag="SV40 polyA "
                     /label="SV40 polyA "
                     /ApEinfo_label="SV40 polyA "
                     /ApEinfo_fwdcolor="#996633"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3194..3199
                     /locus_tag="Spe1"
                     /label="Spe1"
                     /ApEinfo_label="Spe1"
                     /ApEinfo_fwdcolor="#2dbbff"
                     /ApEinfo_revcolor="#2dbbff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaAGATCTc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcAAGCT TGGATCCGAC CATGGTGAGC AGAAGACCCT
     1081 GGCTGCTGGC CCTGGCCCTG GCCGTGGCCC TGGCCGCCGG CAGCGCCGGC GCCAGCACCG
     1141 GCAGCGACGC CACCGTGCCC GTGGCCACCC AGGACGGCCC CGACTACGTG TTCCACAGAG
     1201 CCCACGAGAG AATGCTGTTC CAGACCAGCT ACACCCTGGA GAACAACGGC AGCGTGATCT
     1261 GCATCCCCAA CAACGGCCAG TGCTTCTGCC TGGCCTGGCT Gctgcacagc agaggcacac
     1321 caggagaaaa gatcggcgcc caggtctgcc agtggattgc tttcagcatc gccatcgTcc
     1381 tgctgacatt ctacggcttc agcgcctgga aggccacttg cggttgggag gaggtctacg
     1441 tctgttgcgt cgaggtgctg ttcgtgaccc tggagatctt caaggagttc agcagccccg
     1501 ccacagtgta cctgtctacc ggcaaccacg cctattgcct gcgctacttc gagtggctgc
     1561 tgtcttgccc cgtgatcctg atcCGCctga gcaacctgag cggcctgaag aacgactaca
     1621 gcaagcggac catgggcctg atcgtgtctt gcgtgggaat gatcgtgttc ggcatggccg
     1681 caggactggc taccgattgg ctcaagtggc tgctgtatat cgtgtcttgc atctacggcg
     1741 gctacatgta cttccaggcc gccaagtgct acgtggaagc caaccacagc gtgcctaaag
     1801 gccattgccg catggtcgtg aagctgatgg cctacgcttt cttcgcctct tggggcatgt
     1861 acccaatcct ctgggcagtg ggaccagaag gactgctgaa gctgagccct tacgccaaca
     1921 gcatcggcca cagcatctgc gacatcatcg ccaaggagtt ttggaccttc ctggcccacc
     1981 acctgaggat caagatccac gagcacatcc tgatccacgg cgacatccgg aagaccacca
     2041 agatggagat cggaggcgag gaggtggaag tggaagagtt cgtggaggag gaggacgagg
     2101 acacagtgct cgagAAGAGC AGGATCACCA GCGAGGGCGA GTACATCCCC CTGGACCAGA
     2161 TCGACATCAA CGTGatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2221 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2281 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2341 tgccctggcc caccctcgtg accaccttcg gctacggcct gatgtgcttc gcccgctacc
     2401 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2461 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2521 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2581 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2641 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2701 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2761 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     2821 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2881 agctgtacaa gTAaTCTAGA ataatcaacc tctggattac aaaatttgtg aaagattgac
     2941 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3001 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3061 agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3121 gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt
     3181 atttgtaacc atcACTAGTt aagatacatt gatgagtttg gacaaaccac aactagaatg
     3241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     3301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     3361 ggggagatgt gggaggtttt ttaaaatcga taaggatctt cctagagcat ggctacgtag
     3421 ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca
     3481 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     3541 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat taacctaatt
     3601 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     3661 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3721 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     3781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3961 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4021 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4081 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4141 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4201 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4261 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4321 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4381 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4441 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4501 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4561 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4621 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4681 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4741 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4801 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4861 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4921 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4981 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5041 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5101 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5161 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5221 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5281 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5521 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6001 cttttgctgg ccttttgctc acatg   
//
