LOCUS       AAV_hSyn_ZipACR_        6022 bp ds-DNA     circular     16-MAY-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    570..1041
                     /locus_tag="hSyn"
                     /ApEinfo_fwdcolor="#a3ff98"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2001..2693
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4344..5203
                     /locus_tag="Amp"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    378..509
                     /locus_tag="5'ITR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1046..1051
                     /locus_tag="HindIII"
                     /ApEinfo_fwdcolor="#fff974"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3451..3581
                     /locus_tag="3' ITR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3197..3382
                     /locus_tag="SV40 polyA "
                     /ApEinfo_fwdcolor="#996633"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2892..2897
                     /locus_tag="XbaI"
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3763..4080
                     /locus_tag="f1 origin"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2898..2924
                     /locus_tag="WPRE-start"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2694..2888
                     /locus_tag="Kv2.1_trafficking"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2898..3190
                     /locus_tag="WPRE"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1728..1730
                     /locus_tag="F224"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1053..1058
                     /locus_tag="BamHI"
                     /ApEinfo_fwdcolor="#7cff9f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2001..2693
                     /codon_start="1"
                     /product="bright monomeric red fluorescent protein evolved
                     from a synthetic template (Bindels et al., 2016)"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDG
                     GAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGD
                     IKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSE
                     GRHSTGGMDELYK"
                     /locus_tag="mScarlet"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2399..2418
                     /note="mCherry, forward primer"
                     /locus_tag="mCherry-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1725..1727
                     /locus_tag="S223"
                     /ApEinfo_fwdcolor="#21fe06"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1059..1975
                     /locus_tag="ZipACR"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1976..1991
                     /locus_tag="ZipACR(1)"
                     /ApEinfo_label="ZipACR"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1707..1709
                     /locus_tag="F217"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1743..1745
                     /locus_tag="V229"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1509..1511
                     /locus_tag="I151V"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaAGATCTc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcAAGCT TGGGATCCAT GGCCGCCCTC CATCAGGCTC
     1081 TGGAGGAGCT GGGACAGGGA GCCAGCAGGC ACCTCCTGTA CAGAGCCCCC GAGGAGATGG
     1141 TGTATGGCAA GTGGGTGGAG GCCAACCCTA GGTGCATCGA ACTGCACGAC CACTCCGACT
     1201 ACGTGTACGT GTTCCAGCTG TGCTTCGCCG TGGTGTGCGC CtGCCAGGTG ATCTTCATGT
     1261 TCACCAGGGC CCCCAACGTG GGCTGGGAAG CCATCTATCT GCCCCTGGCC GAGGTCGTGA
     1321 CCTACAGCAT TGCCGCCAAC GGAGAGGGCG TGCTGAGGAT GGCCGACGGC AGGTACTTCA
     1381 ATTTCGCCAA ACTGGCCGGA TGGGCCGTGT GCTGCCCCAT TATGCTCATC CAGATCGGAG
     1441 GCATGGCCCA GATCAAGTAC AGGACCATCC CCCTGAACAA CGTGGTGCTG GCCGCTAGCC
     1501 TGAATAGGgT CATCTTCGGC ATGGCCAGCG CCATCACAGC CTCCGACCCT GCTAGATGGG
     1561 GCTTTTACTT CTGCGCCTGG ATCTGTTACC TGACCGAGGT CGGCATCACC CTGACCATCA
     1621 TGGCCGTGGC CATCAGCGAC TTCAGCAAGA TCAAGACAGA GCTCGGCCAG TGGGTGGTGG
     1681 GCCGTATTCA AACCATGAGG ATCATCTTTC TGGTCGCCTG GACCAGCTTC CCCGTCGTGT
     1741 GGGTGCTGGG CTACACCGGA TTCTGCGTGA TCCACGAGGA CTACATCGCC CTGCTGTATC
     1801 TGTTCGCCGA CCTGCTCTCC AAGAACACCT GGGGAGTGAT GATGTGGCAT ACCACCTGGG
     1861 TGAAACTCAA CGGCAAATGG GACAGGGAAT TTGCCGCCGC CGGAGGACAC GAGGCTCTGA
     1921 AGAAGGCCCT GGAGCAGGAC GTGGAAATCG GCGCCGGCGA GAAGAACCAG AACCAGCTGA
     1981 CCGCCAGGCA GCTCGAGATG gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt
     2041 tcaaggtgca catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
     2101 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     2161 tgcccttctc ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcacca
     2221 agcaccccgc cgacatcccc gactactata agcagtcctt ccccgagggc ttcaagtggg
     2281 agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt gacccaggac acctccctgg
     2341 aggacggcac cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc
     2401 ccgtaatgca gaagaagaca atgggctggg aagcgtccac cgagcggttg taccccgagg
     2461 acggcgtgct gaagggcgac attaagatgg ccctgcgcct gaaggacggc gggcgctacc
     2521 tggcggactt caagaccacc tacaaggcca agaagcccgt gcagatgccc ggcgcctaca
     2581 acgtggaccg caagttggac atcacctccc acaacgagga ctacaccgtg gtggaacagt
     2641 acgaacgctc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagCAGTCCC
     2701 AACCCATACT GAACACGAAG GAAATGGCTC CTCAAAGTAA ACCGCCCGAA GAACTCGAaA
     2761 TGAGTTCAAT GCCATCACCT GTGGCTCCGC TTCCCGCCCG CACGGAAGGT GTCATCGACA
     2821 TGCGAAGCAT GTCTAGTATT GACTCATTTA TTAGCTGCGC CACCGATTTC CCTGAAGCTA
     2881 CTCGGTTCTA aTCTAGAata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2941 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3001 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt
     3061 tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3121 gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt
     3181 tgtaaccatc ACTAGTtaag atacattgat gagtttggac aaaccacaac tagaatgcag
     3241 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3301 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3361 gagatgtggg aggtttttta aaatcgataa ggatcttcct agagcatggc tacgtagata
     3421 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc
     3481 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     3541 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac
     3601 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     3661 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     3721 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     3781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3901 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3961 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4021 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4081 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4141 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4201 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4261 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4321 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4381 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4441 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4501 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4561 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4621 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4681 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4741 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4801 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4861 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4921 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4981 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5041 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5101 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5161 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5221 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5281 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5341 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5401 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5461 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5521 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5581 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5641 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5701 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5761 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5821 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5881 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5941 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6001 ttgctggcct tttgctcaca tg   
//
