LOCUS       pTET_O_FOXP1_VH2       11187 bp DNA     circular SYN 27-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2024 from SnapGene Viewer 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11187
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        100..479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        481..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     634..654
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             697..877
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    924..1049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1542..1775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1960..2004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2302..2419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2476..2494,2495..2517,2518..2536,2537..2559,2560..2578,
                     2579..2601,2602..2620,2621..2643,2644..2662,2663..2685,
                     2686..2704,2705..2727,2728..2746)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2779..2817
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2779..2788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2814..2838
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2919..5036
                     /codon_start=1
                     /product="From addgene 16362"
                     /label=FOXP1A
                     /translation="MQESGSETKSNGSAIQNGSSGGNHLLECGALRDTRSNGEAPAVDL
                     GAADLAHVQQQQQQALQVARQLLLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ
                     QQVSGLKSPKRNDKQPALQVPVSVAMMTPQVITPQQMQQILQQQVLSPQQLQVLLQQQQ
                     ALMLQQQQLQEFYKKQQEQLQLQLLQQQHAGKQPKEQQQVATQQLAFQQQLLQMQQLQQ
                     QHLLSLQRQGLLTIQPGQPALPLQPLAQGMIPTELQQLWKEVTSAHTAEETTSSNHSSL
                     DLTSTCVSSSAPSKSSLIMNPHASTNGQLSVHTPKRESLSHEEHPHSHPLYGHGVCKWP
                     GCEAVCDDFPAFLKHLNSEHALDDRSTAQCRVQMQVVQQLELQLAKDKERLQAMMTHLH
                     VKSTEPKAAPQPLNLVSSVTLSKSASEASPQSLPHTPTTPTAPLTPVTQGPSVITTTSM
                     HTVGPIRRRYSDKYNVPISSADIAQNQEFYKNAEVRPPFTYASLIRQAILESPEKQLTL
                     NEIYNWFTRMFAYFRRNAATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEVEFQKRRPQ
                     KISGNPSLIKNMQSSHAYCTPLNAALQASMAENSIPLYTTASMGNPTLGSLASAIREEL
                     NGAMEHTNSNESDSSPGRSPMQAVHPIHVKEEPLDPEEAEGPLSLVTTANHSPDFDHDR
                     DYEDEPVNEDME"
     CDS             5043..5096
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5103..5702
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5103..5122)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5599..5619
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5741..6329
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5794..5814)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6212..6223)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6332..6348)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6333..6349)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6854..7034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7060..7077)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7066..7290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7336..7764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7423..7442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7633..7654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7773..7793)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7778..8107
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7958..8093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8020..8039
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8155..8202
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8221..8595
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8725..8846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8762..8781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8816..8835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8895..8911)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8895..8911)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8908..8930)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8919..8935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8943..8949,8950..8967,8968..8973))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8988..9009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9126..9143)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9297..9885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9377..9396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10056..10847,10848..10916))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10679..10698
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10917..11021)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11096..11115)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
       61 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      661 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      721 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      781 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      841 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      901 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      961 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1021 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1081 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1141 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1201 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1261 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1321 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1381 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2221 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     2281 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2341 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2401 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaga
     2461 tctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2521 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2581 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2641 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2701 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2761 gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
     2821 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2881 caccgggacc gatccagcct ccgcggcccc gcgccaccat gcaagaatct gggtctgaga
     2941 caaaaagtaa cggatcggcc atccagaacg ggtccagcgg tggcaaccac ttactagagt
     3001 gcggggcact tcgtgacact cggtccaacg gagaggcacc agcggtggac ctgggggcag
     3061 ccgaccttgc ccacgtccag cagcagcaac agcaggccct gcaggtggca aggcagctcc
     3121 tccttcagca gcagcaacag cagcagcagc aacagcagca gcagcagcag cagcaacagc
     3181 agcaacagca gcagcaacaa cagcagcagc agcaacagca gcagcagcag cagcaagtta
     3241 gtggattaaa gtctcccaag aggaatgaca agcaaccagc tcttcaggtt cccgtgtcag
     3301 tggctatgat gacacctcag gttatcactc ctcaacaaat gcagcagatc ctccagcagc
     3361 aggtgctgag cccccagcag ctccaggttc tcctccagca gcagcaggcc ctcatgcttc
     3421 aacagcagca gcttcaagaa ttttataaaa aacaacagga acagttgcag cttcaacttc
     3481 tccaacagca acatgctgga aaacagccga aagagcagca gcaggtggct acccagcagt
     3541 tggctttcca gcagcagctt ctccagatgc agcagctgca gcagcagcac ctcttgtctc
     3601 tccagcgcca aggcctccta acaattcagc cggggcagcc tgcccttccc ctccaacccc
     3661 tcgctcaagg catgattcca acggaactgc agcagctctg gaaagaagtg acaagtgccc
     3721 acactgcaga ggaaaccaca agcagcaacc acagcagcct agacctgacc agcacatgtg
     3781 tctcgtcctc ggcaccttcc aagtcctccc taatcatgaa cccgcatgcc tctaccaatg
     3841 gacagctctc ggtccacact cccaaaaggg aaagcttgtc ccacgaggag cacccccaca
     3901 gccaccctct ctatggacat ggcgtatgca agtggccagg ctgtgaggcg gtttgtgacg
     3961 acttcccagc ctttctaaaa catctcaaca gtgagcatgc gctggacgat agaagcacag
     4021 ctcaatgtag agtacaaatg caggttgtac agcagttaga gctacagctt gcaaaagaca
     4081 aagagcgcct gcaagccatg atgacccacc tgcatgtgaa gtctacagaa cccaaagctg
     4141 cccctcagcc cctgaatctg gtatcaagtg tcaccctctc caagtctgcc tcagaggctt
     4201 ctccacagag cttacctcat actccaacaa cccccaccgc ccccctgact cctgtcaccc
     4261 aaggcccctc cgtcatcacc accaccagca tgcacacggt gggacctatc cgcaggcggt
     4321 actcagacaa atacaacgtg cccatttctt cagcagatat tgcgcagaac caagaatttt
     4381 ataagaacgc ggaagttaga ccaccattta catatgcatc tttaatcagg caggccattc
     4441 tcgaatctcc agaaaagcag ctaacactaa acgaaatcta taactggttc acacgaatgt
     4501 ttgcttactt ccgacgcaat gcagccacgt ggaagaatgc agtgcgtcat aatcttagtc
     4561 tccacaagtg ttttgtgcga gtagagaacg ttaaaggggc agtatggaca gtggatgaag
     4621 tagagttcca aaaacggagg ccacaaaaga tcagtggtaa cccttccctt attaaaaaca
     4681 tgcagagcag ccacgcctac tgcacacctc tcaatgcagc tttacaggct tccatggctg
     4741 agaatagtat acctctgtac actaccgctt ccatgggaaa tcccactctg ggcagcctgg
     4801 ccagtgccat ccgggaggag ctgaacgggg ccatggagca caccaacagc aacgagagtg
     4861 acagcagtcc aggcagatcc cctatgcaag ctgtgcaccc catacacgtc aaagaagaac
     4921 ccctcgaccc cgaggaagct gaaggccctc tgtccttagt gacaacagcc aaccacagtc
     4981 cagattttga ccatgacaga gattacgaag acgaaccagt aaatgaggac atggagtcta
     5041 gagagggcag gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc ggcccactcg
     5101 agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5161 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5221 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5281 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5341 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5401 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5461 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5521 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5581 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5641 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5701 gattaattaa gctagctaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     5761 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5821 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5881 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5941 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6001 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6061 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6121 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6181 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6241 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6301 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     6361 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     6421 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     6481 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6541 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     6601 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     6661 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     6721 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     6781 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     6841 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7021 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     7081 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     7141 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     7201 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     7261 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     7321 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7381 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7441 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7501 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7561 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7621 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7681 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7741 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7801 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7861 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7921 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7981 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8041 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8101 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     8161 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     8221 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     8281 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     8341 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     8401 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     8461 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     8521 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     8581 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8641 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8701 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8821 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8881 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8941 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9001 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9061 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9121 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9181 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9721 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11041 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    11101 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
    11161 ctgagtagtg cgcgagcaaa atttaag
//
