LOCUS       pTet_O_RUNX1T1_p       10794 bp DNA     circular SYN 16-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   TBB
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2024 from SnapGene Viewer 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10794
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        84..660
                     /label=CMV_immearly_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    163..450
                     /label=CAG_enhancer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    617..637
                     /label=CMV_fwd_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    680..860
                     /label=HIV-1_5_LTR
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    680..860
                     /label=truncHIV-1_3_LTR
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    971..1015
                     /label=HIV-1_psi_pack
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    1525..1758
                     /label=RRE
                     /note="/translation=MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAV
                     GIGAL 
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL 
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE 
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL 
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN 
                     GSALRAPILQTNGSIHPQF*"
     misc_feature    2289..2304
                     /label=cPPT
                     /note="/translation=MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAV
                     GIGAL 
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL 
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE 
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL 
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN 
                     GSALRAPILQTNGSIHPQF*"
     misc_feature    2442..2759
                     /label=TRE
                     /note="/translation=MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAV
                     GIGAL 
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL 
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE 
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL 
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN 
                     GSALRAPILQTNGSIHPQF*"
     promoter        2760..2879
                     /label=CMV2_promoter
                     /note="/translation=MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAV
                     GIGAL 
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL 
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE 
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL 
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN 
                     GSALRAPILQTNGSIHPQF*"
     primer_bind     2793..2817
                     /label=LNCX_F
     promoter        2896..2904
                     /label=Kozak
     CDS             2902..4626
                     /codon_start=1
                     /product="From addgene 12431"
                     /label=RUNX1T1
                     /translation="MRTEKHSTMPDSPVDVKTQSRLTPPTMPPPPTTQGAPRTSSFTPT
                     TLTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTL
                     QQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLL
                     QRELLHCARLAKQNPAQYLAQHEQLLLDASTTSPVDSSELLLDVNENGKRRTPDRTKEN
                     GFDREPLHSEHPSKRPCTISPGQRYSPNNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHH
                     YRDSYRHPSHRDLRDRNRPMGLHGTRQEEMIDHRLTDREWAEEWKHLDHLLNCIMDMVE
                     KTRRSLTVLRRCQEADREELNYWIRRYSDAEDLKKGGGSSSSHSRQQSPVNPDPVALDA
                     HREFLHRPASGYVPEEIWKKAEEAVNEVKRQAMTELQKAVSEAERKAHDMITTERAKME
                     RTVAEAKRQAAEDALAVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEK
                     HHHICGQTLQAQQQGDTPAVSSSVTPNSGAGSPMDTPPAATPRSTTPGTPSTIETTPR"
     CDS             4656..5300
                     /codon_start=1
                     /label=Puro
                     /translation="MRGRGGKSRPTRDDRVQAHGAPRHPRRRPQGRTHPRRRVRRLPRH
                     APHRRSGPPHRAGHRAARTLPHARRARHRQGVGRGRRRRGGGLDHAGERRSGGGVRRDR
                     PAHGRVERFPAGRAATDGRPPGAAPAQGARVVPGHRRRLARPPGQGSGQRRRAPRSGGG
                     RARRGARLPGDLRAPQPPLLRAARLHRHRRRRGARRTAHLVHDPQARCLIN"
     misc_feature    5333..5920
                     /label=WPRE
                     /note="/translation=MRGRGGKSRPTRDDRVQAHGAPRHPRRRPQGRTHPRRRV
                     RRLPR 
                     HAPHRRSGPPHRAGHRAARTLPHARRARHRQGVGRGRRRRGGGLDHAGERRSGGGVRR 
                     DRPAHGRVERFPAGRAATDGRPPGAAPAQGARVVPGHRRRLARPPGQGSGQRRRAPRS 
                     GGGRARRGARLPGDLRAPQPPLLRAARLHRHRRRRGARRTAHLVHDPQARCLIN*"
     primer_bind     complement(5384..5404)
                     /label=WPRE_R
     misc_feature    6107..6128
                     /label=U3PPT
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    6107..6122
                     /label=cPPT
                     /label=cPPT(1)
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    6444..6624
                     /label=truncHIV-1_3_LTR
                     /label=truncHIV-1_3_LTR(1)
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    6444..6624
                     /label=HIV-1_5_LTR
                     /label=HIV-1_5_LTR(1)
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    complement(6650..6667)
                     /label=BGH_rev_primer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     terminator      6653..6880
                     /label=bGH_PA_terminator
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     rep_origin      6943..7249
                     /label=f1_origin
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    complement(7363..7383)
                     /label=pBABE_3_primer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    complement(7369..7584)
                     /label=SV40_enhancer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     promoter        7381..7649
                     /label=SV40_promoter
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     rep_origin      7548..7625
                     /label=SV40_origin
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    7610..7629
                     /label=SV40pro_F_primer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     promoter        7743..7810
                     /label=EM7_promoter
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    7811..8185
                     /label=sh_ble
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     gene            7811..8182
                     /gene="bleo"
                     /label=bleo
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     terminator      8318..8437
                     /label=SV40_PA_terminator
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    8406..8425
                     /label=EBV_rev_primer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     promoter        complement(8481..8499)
                     /label=M13_reverse_primer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    complement(8498..8520)
                     /label=M13_pUC_rev_primer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     promoter        complement(8534..8563)
                     /label=lac_promoter
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     CDS             complement(9646..10506)
                     /codon_start=1
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10548..10576)
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
       61 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
      721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      841 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
      901 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
      961 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     1021 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     1081 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     1141 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1201 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1261 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1321 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     1381 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1441 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1501 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1561 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1621 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1681 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1741 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1801 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1861 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1921 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1981 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2041 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2101 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2161 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2221 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     2281 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2341 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2401 ttcgggttta ttacagggac agcagagatc cagtttggtt actcgagttt accactccct
     2461 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2521 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2581 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2641 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     2701 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagt
     2761 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2821 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2881 cgcggccccg aattcgccac catgcgtact gagaagcact ccacaatgcc agactcacct
     2941 gtggatgtga agacgcaatc taggctgact cctccaacaa tgccacctcc cccaactact
     3001 caaggagctc caagaaccag ttcatttaca ccgacaacgt taactaatgg cacgagccat
     3061 tctcctacag ccttgaatgg cgccccctca ccacccaatg gcttcagcaa tgggccttcc
     3121 tcttcttcct cctcctctct ggctaatcaa cagctgcccc cagcctgtgg tgccaggcaa
     3181 ctcagcaagc tgaaaaggtt ccttactacc ctgcagcagt ttggcaatga catttcaccc
     3241 gagataggag aaagagttcg caccctcgtt ctgggactag tgaactccac tttgacaatt
     3301 gaagaatttc attccaaact gcaagaagct actaacttcc cactgagacc ttttgtcatc
     3361 ccatttttga aggccaactt gcccctgctg cagcgtgagc tcctccactg cgcaagactg
     3421 gccaaacaga accctgccca gtacctcgcc cagcatgaac agctgcttct ggatgccagc
     3481 accacctcac ctgttgactc ctcagagctg cttctcgatg tgaacgaaaa cgggaagagg
     3541 cgaactccag acagaaccaa agaaaatggc tttgacagag agcctttgca ctcagaacat
     3601 ccaagcaagc gaccatgcac tattagccca ggccagcggt acagtccaaa taacggctta
     3661 tcctaccagc ccaatggcct gcctcaccct accccacctc cacctcagca ttaccgtttg
     3721 gatgatatgg ccattgccca ccactacagg gactcctatc gacaccccag ccacagggac
     3781 ctcagggaca gaaacagacc tatggggttg catggcacac gtcaagaaga aatgattgat
     3841 cacagactaa cagacagaga atgggcagaa gagtggaaac atcttgacca tctgttaaac
     3901 tgcataatgg acatggtaga aaaaacaagg cgatctctca ccgtactaag gcggtgtcaa
     3961 gaagcagacc gggaagaatt gaattactgg atccggcggt acagtgacgc cgaggactta
     4021 aaaaaaggtg gcggcagtag cagcagccac tctaggcagc agagtcccgt caacccagac
     4081 ccagttgcac tagacgcgca tcgggaattc cttcacaggc ctgcgtctgg atacgtgcca
     4141 gaggagatct ggaagaaagc tgaggaggcc gtcaatgagg tgaagcgcca ggcgatgacg
     4201 gagctgcaga aggccgtgtc tgaggcggag cggaaagccc acgacatgat cacaacagag
     4261 agggccaaga tggagcgcac ggtcgccgag gccaaacggc aggcggcgga ggacgcactg
     4321 gcagttatca atcagcagga ggattcaagc gagagttgct ggaattgtgg ccgtaaagcg
     4381 agtgaaacct gcagtggctg taacacagcc cgatactgtg gctcattttg ccagcacaaa
     4441 gactgggaga agcaccatca catctgtgga cagaccctgc aggcccagca gcagggagac
     4501 acacctgcag tcagctcctc tgtcacgccc aacagcgggg ctgggagccc gatggacaca
     4561 ccaccagcag ccactccgag gtcaaccacc ccgggaaccc cttccaccat agagacaacc
     4621 cctcgctcta gagagggcag gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc
     4681 ggcccactcg agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     4741 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     4801 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     4861 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     4921 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     4981 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5041 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5101 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5161 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5221 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5281 cccggtgcct gattaattaa gctagctaat tcgatatcaa gcttatcgat aatcaacctc
     5341 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5401 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5461 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5521 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5581 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5641 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5701 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5761 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5821 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5881 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     5941 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6001 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6061 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6121 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6181 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     6241 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     6301 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     6361 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     6421 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6481 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6541 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6601 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     6661 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6721 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6781 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6841 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6901 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6961 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7021 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7081 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7141 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7201 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7261 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7321 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     7381 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7441 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7501 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7561 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7621 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7681 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     7741 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     7801 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     7861 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     7921 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     7981 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     8041 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     8101 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     8161 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     8221 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8281 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8341 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8401 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     8461 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8521 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8581 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8641 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8821 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9061 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9301 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9541 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9601 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9661 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9721 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9781 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9841 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9901 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9961 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10021 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10081 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10141 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10201 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10261 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10321 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10381 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10441 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10501 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10561 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10621 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
    10681 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    10741 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggc
//
