LOCUS       Exported                6226 bp DNA     circular SYN 01-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   varohn
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2024 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..119
                     /label=delta11nt AAV-2 ITR
                     /label=nonstandard type:  aav-2_itr
                     /note="5'- and 3'-ITR, A' shorter than in wtAAV-2"
                     /note="/Transferred_From=delta11nt AAV-2 ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=Melanie"
     promoter        148..617
                     /label=hSyn1 promoter-470
                     /note="human Synapsin 1 promoter"
                     /note="/Transferred_From=hSyn1 promoter-470"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     primer_bind     606..626
                     /label=p433_hSyn1_SEQ_short_R
     primer_bind     611..639
                     /label=p433_hSyn1_SEQ_F
     intron          635..767
                     /label=chI
                     /note="Promega's chimeric intron"
                     /note="/Transferred_From=chI"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     primer_bind     complement(776..804)
                     /label=161_AAV_hSyn_rev
     primer_bind     777..816
                     /label=162_ADRA1Awhale_AAV_for
     primer_bind     787..819
                     /label=ADRA1a_whale_BamHI_F
     primer_bind     787..819
                     /label=Primer 2
     primer_bind     798..820
                     /label=337_nLightR_attB_for
     CDS             805..875
                     /codon_start=1
                     /label=secretory sequence
                     /translation="MKTIIALSYIFCLVFADYKDDDD"
     CDS             805..875
                     /codon_start=1
                     /label=secretory sequence
                     /translation="MKTIIALSYIFCLVFADYKDDDD"
     misc_feature    805..852
                     /label=HA secretory sequence_misc_feature
                     /note="note=""HA secretory sequence"" - 23..70"
     misc_feature    805..852
                     /label=HA secretory sequence_misc_feature
                     /note="note=""HA secretory sequence"" - 23..70"
     CDS             853..875
                     /label=FLAG_CDS
                     /note="codon_start=1 - note=""FLAG"" - 
                     translation=""DYKDDDDA"" - 71..94"
     CDS             853..875
                     /label=FLAG_CDS
                     /note="codon_start=1 - note=""FLAG"" - 
                     translation=""DYKDDDDA"" - 71..94"
     primer_bind     862..894
                     /label=158_ADRA1Awhale_smBiT_for
     CDS             877..1497
                     /codon_start=1
                     /label=ADRA1A
                     /translation="VFLSGNASDSSNCTHPPPPVNISKAILLGVILGGLILFGVLGNIL
                     VILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFEILGYWAFGRVFCNIWAAV
                     DVLCCTASIMGLCLISIDRYWAISSPERYERKMTPKAAFIALLCVWALSLVISIGPLFG
                     WRQPAPEDETICQINEEPGYVLFSALGSFYVPLTIILVMYCRVY"
     CDS             877..1497
                     /codon_start=1
                     /label=ADRA1A
                     /translation="VFLSGNASDSSNCTHPPPPVNISKAILLGVILGGLILFGVLGNIL
                     VILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFEILGYWAFGRVFCNIWAAV
                     AVLCCTASIMGLCLISIDRYWAISSPERYERKMTPKAAFIALLCVWALSLVISIGPLFG
                     WRQPAPEDETICQINEEPGYVLFSALGSFYVPLTIILVMYCRVY"
     primer_bind     877..903
                     /label=443_EGFP_fusion_pl_for
     primer_bind     complement(1162..1191)
                     /label=497_nLightR2_D129A_rev
     primer_bind     complement(1179..1200)
                     /label=499_nLightR2_C133E_rev
     primer_bind     1192..1220
                     /label=498_nLightR2_D129A_for
     primer_bind     1204..1226
                     /label=496_nLightR2_C133E_for
     primer_bind     complement(1227..1257)
                     /label=53_ADRA1Awhale_ICL2G_scan_rev
     CDS             1249..1308
                     /codon_start=1
                     /label=RdLight1
                     /translation="WAISSPERYERKMTPKAAFI"
     CDS             1249..1308
                     /codon_start=1
                     /label=RdLight1
                     /translation="WAISSPERYERKMTPKAAFI"
     primer_bind     1258..1287
                     /label=67_ADRA1Awhale_RdLG_ICL2G_R128K
     primer_bind     1258..1287
                     /label=68_ADRA1Awhale_RdLG_ICL2G_R128E
     primer_bind     1258..1284
                     /label=60_ADRA1Awhale_RdLG_ICL2G_P126A
     primer_bind     1258..1284
                     /label=61_ADRA1Awhale_RdLG_ICL2G_P126K
     primer_bind     1258..1284
                     /label=63_ADRA1Awhale_RdLG_ICL2G_E127A
     primer_bind     1258..1284
                     /label=65_ADRA1Awhale_RdLG_ICL2G_E127E
     primer_bind     1258..1284
                     /label=66_ADRA1Awhale_RdLG_ICL2G_R128A
     primer_bind     1258..1280
                     /label=64_ADRA1Awhale_RdLG_ICL2G_E127K
     primer_bind     1261..1284
                     /label=54_ADRA1Awhale_RdLG_ICL2G_S124A
     primer_bind     1261..1284
                     /label=55_ADRA1Awhale_RdLG_ICL2G_S124K
     primer_bind     1261..1284
                     /label=56_ADRA1Awhale_RdLG_ICL2G_S124E
     primer_bind     1263..1284
                     /label=59_ADRA1Awhale_RdLG_ICL2G_S125E
     primer_bind     1264..1284
                     /label=57_ADRA1Awhale_RdLG_ICL2G_S125A
     primer_bind     1264..1284
                     /label=58_ADRA1Awhale_RdLG_ICL2G_S125K
     primer_bind     1267..1284
                     /label=62_ADRA1Awhale_RdLG_ICL2G_P126E
     CDS             1267..1269
                     /codon_start=1
                     /label=Mutate to A
                     /translation="E"
     CDS             1267..1269
                     /codon_start=1
                     /label=Mutate to A
                     /translation="E"
     primer_bind     complement(1482..1506)
                     /label=203_ADRA1Awhale_TM5ins-3_rev
     primer_bind     complement(1488..1512)
                     /label=200_ADRA1Awhale_TM5ins-1_rev
     primer_bind     complement(1488..1509)
                     /label=202_ADRA1Awhale_TM5ins-2_rev
     primer_bind     complement(1492..1515)
                     /label=204_ADRA1Awhale_TM5ins+1_rev
     primer_bind     complement(1492..1515)
                     /label=205_ADRA1Awhale_TM5ins+2_rev
     primer_bind     complement(1492..1515)
                     /label=206_ADRA1Awhale_TM5ins+3_rev
     primer_bind     1495..1516
                     /label=168_RdLG+1_for
     CDS             1498..2289
                     /codon_start=1
                     /label=RdLight1
                     /translation="RIAQKKLSSFMVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVKT
                     TYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGSL
                     VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLP
                     FAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQ
                     DGVFIYKVKLRGTNFPPDGPVMQKKTMGWEHRKDDLKRETKV"
     CDS             1498..2289
                     /codon_start=1
                     /label=RdLight1
                     /translation="RIAQKKLSSFMVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVKT
                     TYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGSL
                     VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLP
                     FAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQ
                     DGVFIYKVKLRGTNFPPDGPVMQKKTMGWEHRKDDLKRETKV"
     primer_bind     1498..1518
                     /label=93_For_coRdLight_RIAQKQ
     primer_bind     1498..1518
                     /label=7_110_For_coRdLight_RIAQKQ
     primer_bind     complement(1503..1530)
                     /label=453_nLightR_GRAB3m_rev
     primer_bind     complement(1503..1522)
                     /label=215_coRdLight_rev
     primer_bind     complement(1504..1527)
                     /label=70_ADRA1Awhale_RdLGp1_F218L
     primer_bind     complement(1504..1527)
                     /label=224_Blp1_rev
     primer_bind     complement(1504..1524)
                     /label=69_ADRA1Awhale_RdLGp1_F218A
     primer_bind     complement(1504..1524)
                     /label=71_ADRA1Awhale_RdLGp1_F218S
     primer_bind     complement(1504..1524)
                     /label=72_ADRA1Awhale_RdLGp1_F218Q
     primer_bind     complement(1504..1524)
                     /label=75_ADRA1Awhale_RdLGp1_F218D
     primer_bind     complement(1504..1524)
                     /label=76_ADRA1Awhale_RdLGp1_F218E
     primer_bind     complement(1504..1524)
                     /label=77_ADRA1Awhale_RdLGp1_F218R
     primer_bind     complement(1504..1524)
                     /label=78_ADRA1Awhale_RdLGp1_F218K
     primer_bind     complement(1504..1524)
                     /label=79_ADRA1Awhale_RdLGp1_F218H
     primer_bind     complement(1505..1526)
                     /label=74_ADRA1Awhale_RdLGp1_F218F
     primer_bind     complement(1505..1525)
                     /label=73_ADRA1Awhale_RdLGp1_F218Y
     CDS             1513..1515
                     /codon_start=1
                     /label=QtoK GRABDA3m
                     /translation="K"
     CDS             1513..1515
                     /codon_start=1
                     /label=QtoK GRABDA3m
                     /translation="K"
     primer_bind     1515..1542
                     /label=456_nLightR_mutins_for
     CDS             1525..2271
                     /codon_start=1
                     /label=cpmApple
                     /translation="FMVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVKTTYKAKKPVQ
                     LPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGSLVSKGEEDNM
                     AIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQ
                     FMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGVFIYKVK
                     LRGTNFPPDGPVMQKKTMGWEHRKDDL"
     CDS             1525..2271
                     /codon_start=1
                     /label=cpmApple
                     /translation="FMVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVKTTYKAKKPVQ
                     LPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGSLVSKGEEDNM
                     AIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQ
                     FMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGVFIYKVK
                     LRGTNFPPDGPVMQKKTMGWEHRKDDL"
     CDS             1528..1530
                     /codon_start=1
                     /label=HtoM GRABDA3m
                     /translation="M"
     CDS             1528..1530
                     /codon_start=1
                     /label=HtoM GRABDA3m
                     /translation="M"
     primer_bind     1530..1555
                     /label=88_ADRA1Awhale_RdLGp1_H219R
     primer_bind     1531..1555
                     /label=80_ADRA1Awhale_RdLGp1_H219A
     primer_bind     1531..1555
                     /label=81_ADRA1Awhale_RdLGp1_H219L
     primer_bind     1531..1555
                     /label=82_ADRA1Awhale_RdLGp1_H219S
     primer_bind     1531..1555
                     /label=83_ADRA1Awhale_RdLGp1_H219Q
     primer_bind     1531..1555
                     /label=84_ADRA1Awhale_RdLGp1_H219Y
     primer_bind     1531..1555
                     /label=85_ADRA1Awhale_RdLGp1_H219F
     primer_bind     1531..1555
                     /label=86_ADRA1Awhale_RdLGp1_H219D
     primer_bind     1531..1555
                     /label=87_ADRA1Awhale_RdLGp1_H219E
     primer_bind     1531..1555
                     /label=89_ADRA1Awhale_RdLGp1_H219K
     primer_bind     1531..1552
                     /label=90_ADRA1Awhale_RdLGp1_H219H
     primer_bind     complement(1557..1581)
                     /label=92_ADRA1Awhale_RdLGp1_ICL2G_K237A
     primer_bind     1584..1603
                     /label=91_ADRA1Awhale_RdLGp1_ICL2G_K237A
     primer_bind     complement(1669..1698)
                     /label=410_cpmApple_Delta1_rev
     misc_feature    1699..1947
                     /label=Delta1
     misc_feature    1699..1947
                     /label=Delta1
     primer_bind     1948..1972
                     /label=409_cpmApple_Delta1_for
     primer_bind     complement(2048..2073)
                     /label=460_nLightR_L435S_rev
     CDS             2074..2076
                     /codon_start=1
                     /label=LtoS GRABDA3m
                     /translation="L"
     CDS             2074..2076
                     /codon_start=1
                     /label=LtoS GRABDA3m
                     /translation="L"
     primer_bind     2076..2100
                     /label=459_nLightR_L435S_for
     primer_bind     complement(2232..2262)
                     /label=455_nLightR_mutins_rev
     primer_bind     2248..2276
                     /label=454_nLightR_GRAB3m_for
     CDS             2254..2256
                     /codon_start=1
                     /label=AtoH GRABDA3m
                     /translation="H"
     CDS             2254..2256
                     /codon_start=1
                     /label=AtoH GRABDA3m
                     /translation="H"
     primer_bind     complement(2259..2289)
                     /label=8_111_Rev_coRdLight_KRETKV
     primer_bind     complement(2265..2292)
                     /label=169_RdLG+1_rev
     primer_bind     2265..2292
                     /label=216_nLightR_for
     primer_bind     complement(2268..2297)
                     /label=226_ScaI_rev
     primer_bind     2272..2298
                     /label=211_ADRA1Awhale_TM6insp1_for
     primer_bind     2272..2298
                     /label=212_ADRA1Awhale_TM6insp2_for
     primer_bind     2272..2298
                     /label=213_ADRA1Awhale_TM6insp3_for
     primer_bind     2275..2299
                     /label=208_ADRA1Awhale_TM6insm1_for
     primer_bind     2278..2302
                     /label=209_ADRA1Awhale_TM6insm2_for
     primer_bind     2281..2308
                     /label=225_ScaI_for
     primer_bind     2281..2304
                     /label=210_ADRA1Awhale_TM6insm3_for
     CDS             2290..2592
                     /codon_start=1
                     /label=ADRA1A
                     /translation="LKTLGIVVGCFVLCWLPFFLVMPIGSFFPDFRPSETVFKIAFWLG
                     YLNSCINPIIYPCSSQEFKKAFQNVLRIQCLRRKQSSKHALGYTLHPPSHALEGQ"
     CDS             2290..2592
                     /codon_start=1
                     /label=ADRA1A
                     /translation="LKTLGIVVGCFVLCWLPFFLVMPIGSFFPDFRPSETVFKIAFWLG
                     YLNSCINPIIYPCSSQEFKKAFQNVLRIQCLRRKQSSKHALGYTLHPPSHALEGQ"
     primer_bind     complement(2418..2451)
                     /label=118_ADRA1Awhale_44_CtermGraft_rev
     primer_bind     complement(2430..2463)
                     /label=105_ADRA1Awhale_TM7_scan_rev
     primer_bind     2464..2499
                     /label=112_ADRA1Awhale_RdLGp1_Q331A
     primer_bind     2464..2499
                     /label=113_ADRA1Awhale_RdLGp1_Q331K
     primer_bind     2464..2499
                     /label=114_ADRA1Awhale_RdLGp1_Q331E
     primer_bind     2465..2497
                     /label=106_ADRA1Awhale_RdLGp1_S329A
     primer_bind     2466..2497
                     /label=107_ADRA1Awhale_RdLGp1_S329K
     primer_bind     2466..2497
                     /label=108_ADRA1Awhale_RdLGp1_S329E
     primer_bind     2470..2502
                     /label=109_ADRA1Awhale_RdLGp1_S330A
     primer_bind     2470..2502
                     /label=110_ADRA1Awhale_RdLGp1_S330K
     primer_bind     2470..2502
                     /label=111_ADRA1Awhale_RdLGp1_S330E
     primer_bind     complement(2520..2544)
                     /label=119_ADRA1Awhale_44_CtermTrunc_rev
     primer_bind     complement(2567..2598)
                     /label=493_232_AAV_rev
     primer_bind     complement(2567..2590)
                     /label=ADRA1A_ERexport_rev
     primer_bind     complement(2567..2589)
                     /label=ADRA1Awhale_minus96_Cterm_trunc_rev
     primer_bind     complement(2567..2589)
                     /label=494_nLightR2_smBiT_rev
     primer_bind     complement(2570..2591)
                     /label=492_232_attB_rev
     misc_feature    2605..3193
                     /label=WPRE(mut6)
                     /label=nonstandard type:  WPRE
                     /note="contains a modified version of the WHV-X 
                     proteinpromoter and start codon (Zanta-Boussif, 2009)"
                     /note="/Transferred_From=WPRE(mut6)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=Melanie"
                     /note="/source=based on J04514.1"
     primer_bind     complement(2672..2691)
                     /label=WHV-5R
     primer_bind     complement(2734..2764)
                     /label=p433_WPRE_SEQ_R
     primer_bind     complement(2768..2788)
                     /label=p443_WPRE_SEQ_short_R
     primer_bind     complement(3046..3056)
                     /label=163_ADRA1Awhale_AAV_rev
     polyA_signal    3208..3436
                     /label=bGHp(A)-229
                     /note="bovine growth hormone poly(A) signal, 229 bp"
                     /note="/Transferred_From=bGHp(A)-229"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(3478..3607)
                     /label=AAV-2 ITR
                     /label=nonstandard type:  AAV-2_ITR
                     /note="A' 15 nucleotides shorter than wtAAV-2 ITR"
                     /note="/Transferred_From=AAV-2 3'-ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=jean-charles"
     rep_origin      3693..4148
                     /label=ori (f1)
                     /note="phage f1 origin of recplication"
                     /note="/Transferred_From=ori (f1)"
                     /note="/Transferred_Similarity=98.68%"
     misc_feature    complement(4190..4464)
                     /label=pUC18-275
                     /note="fragment of pUC18"
                     /note="Geneious type: plasmid backbone"
                     /note="/Transferred_From=pUC18 fragment-275"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=Melanie"
     primer_bind     4259..4278
                     /label=GEX3-rev
     primer_bind     4288..4306
                     /label=GEX3-rev-25
     primer_bind     complement(4337..4355)
                     /label=QE-for
     promoter        4465..4500
                     /label=AmpR promoter-36
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=AmpR promoter-36"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=AmpR promoter-29"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     misc_feature    4535..5395
                     /label=AmpR
                     /label=nonstandard type:  ORF
                     /note="beta-lactamase, confers resistance 
                     againstampicillin"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     primer_bind     complement(4580..4599)
                     /label=AmpStart
     primer_bind     5326..5345
                     /label=AmpStop
     misc_feature    complement(5410..6215)
                     /label=pBR322-806
                     /note="part of plasmid pBR322"
                     /note="Geneious type: plasmid backbone"
                     /note="/Transferred_From=pBR322-806"
                     /note="/Transferred_Similarity=100.00%"
     rep_origin      5566..6154
                     /direction=RIGHT
                     /label=ori (pBR322)
                     /note="pMB1 origin of replication (counter-clockwise)(RNAII
                     -35 to RNA/DNA switch point)"
                     /note="/Transferred_From=ori (pBR322)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
     primer_bind     5577..5588
                     /label=453_nLightR_GRAB3m_rev
     primer_bind     5579..5588
                     /label=215_coRdLight_rev
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgcacg cgtgtgtcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta
      181 ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca
      241 agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca
      301 ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc
      361 cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg
      421 gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag
      481 ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg
      541 gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc
      601 tgagagcgca gtcgagatta attaactcga gcaggtaagt atcaaggtta caagacaggt
      661 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
      721 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg ctagcgttta
      781 aacggtaccg gatcctccgc caccatgaag acgatcatcg ccctgagcta catcttctgc
      841 ctggtgttcg ccgactacaa ggacgatgat gacgcagtct ttctcagtgg gaacgctagt
      901 gactcttcaa attgtaccca cccaccacct cctgttaata tcagcaaggc tattttgctg
      961 ggtgtcatct tgggaggcct gattctgttc ggagtgcttg gtaacattct cgtaattctc
     1021 tcagtagcgt gtcaccgcca tctccacagc gttacccact actatattgt gaacctcgct
     1081 gtagcagacc tgcttctcac gagtacggta ctgccatttt ccgctatctt cgaaattttg
     1141 gggtattggg cctttggacg ggttttttgt aacatctggg ccgctgtggc cgtattgtgt
     1201 tgtacggcat caatcatggg cctgtgcctt atctcaatag atcgatactg ggcgattagt
     1261 tcacccgagc gctacgagag aaagatgact ccgaaggcgg cgttcattgc tctcctttgt
     1321 gtctgggctc tttccttggt tatatccatt gggcccttgt tcggctggcg ccagcccgcc
     1381 ccggaggacg aaacaatatg tcagatcaac gaagagcctg gatacgtcct tttcagcgcc
     1441 ctcggatctt tttatgtccc gttgacaatc atcctggtca tgtattgcag agtttatcgg
     1501 atcgcgcaga aaaagctctc cagcttcatg gtgagcgaga ggatgtatcc tgaggacggt
     1561 gcgctgaaat ccgaaataaa gaaaggcctg cgcctcaaag acgggggcca ctacgccgct
     1621 gaggttaaga caacttacaa ggctaaaaag cctgtccagc tcccgggcgc ctacatagtc
     1681 gacattaaac tggacatagt cagccataac gaggactaca ccatagtcga acagtgtgag
     1741 cgggcggaag gtcgccacag tacaggagga atggacgaat tgtataaagg gggcaccggt
     1801 ggcagcttgg ttagcaaggg agaggaagat aacatggcta ttataaagga gttcatgaga
     1861 tttaaagttc acatggaggg atctgtaaac gggcatgagt ttgagattga gggagaagga
     1921 gaagggcgac cgtacgaggc gttccaaacc gcgaaattga aggtcaccaa gggaggaccc
     1981 cttccgtttg cttgggacat tcttagccca caattcatgt atggcagcaa ggcgtacata
     2041 aagcacccgg cagacatacc cgactacttc aaacttagct ttccagaggg tttccgatgg
     2101 gaaagagtga tgaactttga ggatggtggc atcatacacg tcaatcaaga tagcagcctt
     2161 caggacggag tatttatata caaagtcaaa ctccggggta caaactttcc cccggacggg
     2221 ccagtcatgc agaagaagac tatgggttgg gagcaccgga aagacgactt gaagagggag
     2281 actaaagtcc tcaagacgct cgggatagtt gttggttgtt tcgtactctg ctggttgccc
     2341 tttttcttgg tgatgccgat aggtagcttt tttcccgact tccgaccgag tgagacagta
     2401 ttcaaaatag ctttttggct tggttatttg aactcctgta ttaatcccat tatatatccc
     2461 tgttcatctc aagagtttaa aaaggctttt caaaacgtgt tgcgcataca atgcctgcgc
     2521 agaaagcagt cttctaagca cgcactcggt tatacgctcc accccccatc ccatgctctc
     2581 gagggacagt aaaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2641 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2701 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2761 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2821 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2881 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2941 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3001 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3061 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3121 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3181 gccgcctccc cgctctcgag atacgtacga ctgtgccttc tagttgccag ccatctgttg
     3241 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3301 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3361 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3421 cggtgggctc tatggcttct gaggcggaaa gatctcacgt gcggaccgag cggccgcagg
     3481 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3541 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3601 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3661 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     3781 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3841 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3901 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3961 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4021 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4081 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4141 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4201 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4261 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4321 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4381 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4441 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4501 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4561 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4621 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4681 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4741 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4801 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4861 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4921 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4981 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5041 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5101 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5161 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5221 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5281 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5341 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5401 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5461 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5521 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5701 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5941 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6181 gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcag
//
